Restriction map for
pFN21AE0524
(Circular) (Six-base) MAPSORT of: pFN21AE0524.seq Check: 8711 from: 1 to: 5422
pFN21AE0524 5422 bp
With 182 enzymes: SgfI *
October 20, 2009 10:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2029 2029
Size: 5422
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2568 3232 278
Size: 53 83 186 936 1032 664 2468
Fragments arranged by size:
2468 1032 936 664 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2765 1937
Size: 828 4594
Acc65I G'GTAC_C
Cuts at: 2743 2743
Size: 5422
AclI AA'CG_TT
Cuts at: 1563 3549 3922 1563
Size: 1986 373 3063
Fragments arranged by size:
3063 1986 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2609 3485 4869 5137 1632
Size: 977 876 1384 268 1917
Fragments arranged by size:
1917 1384 977 876 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5225
AflIII A'CryG_T
Cuts at: 1203 2394 4321 5298 1203
Size: 1191 1927 977 1327
Fragments arranged by size:
1927 1327 1191 977
AgeI A'CCGG_T
Cuts at: 4253 4253
Size: 5422
AhdI GACnn_n'nnGTC
Cuts at: 4151 4151
Size: 5422
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5422
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5422
AlwNI CAG_nnn'CTG
Cuts at: 3165 4737 3165
Size: 1572 3850
ApaI G_GGCC'C
Cuts at: 2202 2202
Size: 5422
ApaLI G'TGCA_C
Cuts at: 3479 4635 3479
Size: 1156 4266
ApoI r'AATT_y
Cuts at: 1493 2731 2957 3109 5218 1493
Size: 1238 226 152 2109 1697
Fragments arranged by size:
2109 1697 1238 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3976 160
Size: 872 2944 1606
Fragments arranged by size:
2944 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5422
AvaI C'yCGr_G
Cuts at: 1870 1943 2415 2747 1870
Size: 73 472 332 4545
Fragments arranged by size:
4545 472 332 73
BamHI G'GATC_C
Cuts at: 2752 3119 2752
Size: 367 5055
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2041 2455 2713 2743 4198
Size: 325 217 627 254 414 258 30 1455
Cuts at: 4198 618
Size: 1842
Fragments arranged by size:
1842 1455 627 414 325 258 254 217
30
BanII G_rGCy'C
Cuts at: 1391 2109 2202 2270 2709 2741 1391
Size: 718 93 68 439 32 4072
Fragments arranged by size:
4072 718 439 93 68 32
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2290 2560 928
Size: 971 391 270 3790
Fragments arranged by size:
3790 971 391 270
BbvCI CC'TCA_GC
Cuts at: 2111 2111
Size: 5422
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3613 3647 1903
Size: 34 1676 34 3678
Fragments arranged by size:
3678 1676 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3314 4524 3314
Size: 1210 4212
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5422
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2378 2685 4033
Size: 107 122 71 631 605 706 307 1348
Cuts at: 4033 136
Size: 1525
Fragments arranged by size:
1525 1348 706 631 605 307 122 107
71
BglII A'GATC_T
Cuts at: 5417 5417
Size: 5422
BlpI GC'TnA_GC
Cuts at: 2839 2839
Size: 5422
Bme1580I G_kGCm'C
Cuts at: 1790 2202 3483 4639 1790
Size: 412 1281 1156 2573
Fragments arranged by size:
2573 1281 1156 412
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5422
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3153 4111 442
Size: 416 53 83 399 24 1736 958 1753
Fragments arranged by size:
1753 1736 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5422
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2123 2188 2579 2604 4082 1239
Size: 884 65 391 25 1478 2579
Fragments arranged by size:
2579 1478 884 391 65 25
Bpu10I CC'TnA_GC
Cuts at: 2111 2111
Size: 5422
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3547 4412 4710 3547
Size: 865 298 4259
Fragments arranged by size:
4259 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4085 882
Size: 617 2586 2219
Fragments arranged by size:
2586 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2395 3213 493
Size: 711 1191 818 2702
Fragments arranged by size:
2702 1191 818 711
BsaBI GATnn'nnATC
Cuts at: 5416 5416
Size: 5422
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2319 2565 3229 3611
Size: 53 83 186 936 786 246 664 382
Cuts at: 3611 4246 275
Size: 635 1451
Fragments arranged by size:
1451 936 786 664 635 382 246 186
83 53
BsaWI w'CCGG_w
Cuts at: 2151 3855 4253 4527 4674 5085 2151
Size: 1704 398 274 147 411 2488
Fragments arranged by size:
2488 1704 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5197
Fragments arranged by size:
5197 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2099 1186
Size: 913 4509
BseYI C'CCAG_C
Cuts at: 2335 2497 2524 2641 4625 5121 2335
Size: 162 27 117 1984 496 2636
Fragments arranged by size:
2636 1984 496 162 117 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2117 1163
Size: 573 381 4468
Fragments arranged by size:
4468 573 381
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2028 2281 2289 2872 3633 4661
Size: 275 186 27 253 8 583 761 1028
Cuts at: 4661 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 583 275 253 186 27
8
BsiHKAI G_wGCw'C
Cuts at: 2109 2709 3483 3568 4639 2109
Size: 600 774 85 1071 2892
Fragments arranged by size:
2892 1071 774 600 85
BsmI GAATG_Cn'
Cuts at: 2933 3026 2933
Size: 93 5329
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2109 2202 2270 2709 2741 3483 3568
Size: 399 319 93 68 439 32 742 85
Cuts at: 3568 4639 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 439 399 319 93 85
68 32
BspEI T'CCGG_A
Cuts at: 2151 5085 2151
Size: 2934 2488
BspHI T'CATG_A
Cuts at: 3311 5041 3311
Size: 1730 3692
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2029 2529 2777 844
Size: 1185 500 248 3489
Fragments arranged by size:
3489 1185 500 248
BsrBI CCG'CTC
Cuts at: 1394 1823 3309 1394
Size: 429 1486 3507
Fragments arranged by size:
3507 1486 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3917 4091 65
Size: 1183 2669 174 1396
Fragments arranged by size:
2669 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2044 4066 4253 1700
Size: 110 234 2022 187 2869
Fragments arranged by size:
2869 2022 234 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5422
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5422
BssSI C'ACGA_G
Cuts at: 2513 3482 4494 2513
Size: 969 1012 3441
Fragments arranged by size:
3441 1012 969
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5422
BstBI TT'CG_AA
Cuts at: 4226 4226
Size: 5422
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5422
BstXI CCAn_nnnn'nTGG
Cuts at: 2515 2515
Size: 5422
BstYI r'GATC_y
Cuts at: 1972 2752 3119 3504 3521 4973 5059 5071 5417
Size: 780 367 385 17 1452 86 12 346
Cuts at: 5417 1972
Size: 1977
Fragments arranged by size:
1977 1452 780 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2091 2275 513
Size: 552 939 87 184 3660
Fragments arranged by size:
3660 939 552 184 87
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4683
Fragments arranged by size:
4683 683 56
BtsI GCAGTG_nn'
Cuts at: 2938 3732 3752 2938
Size: 794 20 4608
Fragments arranged by size:
4608 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5422
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5387
DraI TTT'AAA
Cuts at: 2726 2726
Size: 5422
DraIII CAC_nnn'GTG
Cuts at: 2146 2146
Size: 5422
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4429 809
Size: 507 3113 1802
Fragments arranged by size:
3113 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 2278 2286
Size: 54 1425 50 275 18 195 253 8
Cuts at: 2286 2379 2869 3758 5387 8
Size: 93 490 889 1629 43
Fragments arranged by size:
1629 1425 889 490 275 253 195 93
54 50 43 18 8
EagI C'GGCC_G
Cuts at: 1537 1812 2025 2278 2286 2869 1537
Size: 275 213 253 8 583 4090
Fragments arranged by size:
4090 583 275 253 213 8
EarI CTCTTCn'nnn_
Cuts at: 1353 2195 2237 2256 2315 3352 1353
Size: 842 42 19 59 1037 3423
Fragments arranged by size:
3423 1037 842 59 42 19
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4009 4383 4529 387
Size: 1064 138 2420 374 146 1280
Fragments arranged by size:
2420 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2107 2707 2107
Size: 600 4822
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2123 2188 2579 2604 2609 3485 4082
Size: 393 491 65 391 25 5 876 597
Cuts at: 4082 4869 5137 1239
Size: 787 268 1524
Fragments arranged by size:
1524 876 787 597 491 393 391 268
65 25 5
EcoNI CCTnn'n_nnAGG
Cuts at: 2366 2366
Size: 5422
EcoO109I rG'GnC_Cy
Cuts at: 2199 2371 2638 2199
Size: 172 267 4983
Fragments arranged by size:
4983 267 172
EcoRI G'AATT_C
Cuts at: 2731 3109 2731
Size: 378 5044
FspI TGC'GCA
Cuts at: 3928 3928
Size: 5422
HaeII r_GCGC'y
Cuts at: 1678 2477 4569 1678
Size: 799 2092 2531
Fragments arranged by size:
2531 2092 799
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4063 4095 4137 4169 1373
Size: 32 135 32 2491 32 42 32 2626
Fragments arranged by size:
2626 2491 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2428 2766 3012 669
Size: 988 281 490 338 246 3079
Fragments arranged by size:
3079 988 490 338 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5422
HpaI GTT'AAC
Cuts at: 3012 3012
Size: 5422
KpnI G_GTAC'C
Cuts at: 2747 2747
Size: 5422
MfeI C'AATT_G
Cuts at: 3021 3021
Size: 5422
MluI A'CGCG_T
Cuts at: 5298 5298
Size: 5422
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4536 4720 1305
Size: 3231 184 2007
Fragments arranged by size:
3231 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2381 10
Size: 54 1425 343 549 3051
Fragments arranged by size:
3051 1425 549 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2381 2387 2513 3380 3739 3898 518
Size: 648 1215 6 126 867 359 159 2042
Fragments arranged by size:
2042 1215 867 648 359 159 126 6
MspA1I CmG'CkG
Cuts at: 1606 2093 2101 2277 2645 2838 3515 4663 4908
Size: 487 8 176 368 193 677 1148 245
Cuts at: 4908 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 487 368 245 193 176
8
NaeI GCC'GGC
Cuts at: 2046 2046
Size: 5422
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3931
Fragments arranged by size:
3931 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5422
NgoMIV G'CCGG_C
Cuts at: 2044 2044
Size: 5422
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5422
NotI GC'GGCC_GC
Cuts at: 2278 2869 2278
Size: 591 4831
NspI r_CATG'y
Cuts at: 1132 1229 2230 2257 2780 4325 1132
Size: 97 1001 27 523 1545 2229
Fragments arranged by size:
2229 1545 1001 523 97 27
PasI CC'CwG_GG
Cuts at: 1348 2203 2690 1348
Size: 855 487 4080
Fragments arranged by size:
4080 855 487
PciI A'CATG_T
Cuts at: 4321 4321
Size: 5422
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 4520
PfoI T'CCnGG_A
Cuts at: 2556 2556
Size: 5422
PmeI GTTT'AAAC
Cuts at: 2726 2726
Size: 5422
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3491 3523 5004 5036 3491
Size: 32 1481 32 3877
Fragments arranged by size:
3877 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5422
PsiI TTA'TAA
Cuts at: 2992 2992
Size: 5422
PspOMI G'GGCC_C
Cuts at: 2198 2198
Size: 5422
PstI C_TGCA'G
Cuts at: 830 2040 2604 2774 830
Size: 1210 564 170 3478
Fragments arranged by size:
3478 1210 564 170
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5422
PvuII CAG'CTG
Cuts at: 2101 2645 2101
Size: 544 4878
SacI G_AGCT'C
Cuts at: 2109 2709 2109
Size: 600 4822
SacII CC_GC'GG
Cuts at: 2094 2278 2094
Size: 184 5238
SalI G'TCGA_C
Cuts at: 1936 2764 1936
Size: 828 4594
SapI GCTCTTCn'nnn_
Cuts at: 2237 2237
Size: 5422
SbfI CC_TGCA'GG
Cuts at: 2040 2774 2040
Size: 734 4688
ScaI AGT'ACT
Cuts at: 3670 5382 3670
Size: 1712 3710
SfcI C'TryA_G
Cuts at: 826 1045 2036 2600 2770 3905 4586 4777 826
Size: 219 991 564 170 1135 681 191 1471
Fragments arranged by size:
1471 1135 991 681 564 219 191 170
SfiI GGCCn_nnn'nGGCC
Cuts at: 2378 2378
Size: 5422
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5422
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5422
SmaI CCC'GGG
Cuts at: 1872 2749 1872
Size: 877 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3526 4427 4689 820
Size: 197 926 1583 901 262 1553
Fragments arranged by size:
1583 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3213 493
Size: 2720 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5422
SphI G_CATG'C
Cuts at: 1132 2257 2780 1132
Size: 1125 523 3774
Fragments arranged by size:
3774 1125 523
SspI AAT'ATT
Cuts at: 5 52 3346 5
Size: 47 3294 2081
Fragments arranged by size:
3294 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5422
StyI C'CwwG_G
Cuts at: 513 1065 2004 2468 2861 513
Size: 552 939 464 393 3074
Fragments arranged by size:
3074 939 552 464 393
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3449 3466 3619 3804 4249 325
Size: 1255 1869 17 153 185 445 1498
Fragments arranged by size:
1869 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3668 5380 96
Size: 274 80 33 51 1443 1691 1712 138
Fragments arranged by size:
1712 1691 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5382 5414 5382
Size: 32 5390
Tth111I GACn'n_nGTC
Cuts at: 1316 2294 1316
Size: 978 4444
XbaI T'CTAG_A
Cuts at: 2758 2758
Size: 5422
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5422
XmaI C'CCGG_G
Cuts at: 1870 2747 1870
Size: 877 4545
XmnI GAAnn'nnTTC
Cuts at: 3551 3551
Size: 5422
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2566 3230 276
Size: 53 83 186 936 1032 664 2468
Fragments arranged by size:
2468 1032 936 664 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SapI SbfI ScaI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI
KasI NarI NruI NsiI PacI PmlI PpuMI PspXI
PsrI RsrII SanDI SexAI SfoI SrfI SwaI XcmI