Restriction map for
pFN21AE0521
(Circular) (Six-base) MAPSORT of: pFN21AE0521.seq Check: 9612 from: 1 to: 5533
pFN21AE0521 5533 bp
With 182 enzymes: SgfI *
October 20, 2009 10:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2403 3343 278
Size: 53 83 186 936 867 940 2468
Fragments arranged by size:
2468 940 936 867 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2876 1937
Size: 939 4594
Acc65I G'GTAC_C
Cuts at: 2854 2854
Size: 5533
AclI AA'CG_TT
Cuts at: 1563 3660 4033 1563
Size: 2097 373 3063
Fragments arranged by size:
3063 2097 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2342 3596 4980 5248 1632
Size: 710 1254 1384 268 1917
Fragments arranged by size:
1917 1384 1254 710 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5336
AflIII A'CryG_T
Cuts at: 1203 2052 2201 4432 5409 1203
Size: 849 149 2231 977 1327
Fragments arranged by size:
2231 1327 977 849 149
AgeI A'CCGG_T
Cuts at: 4364 4364
Size: 5533
AhdI GACnn_n'nnGTC
Cuts at: 2311 4262 2311
Size: 1951 3582
AleI CACnn'nnGTG
Cuts at: 1166 2300 1166
Size: 1134 4399
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2495 1184
Size: 1311 4222
AlwNI CAG_nnn'CTG
Cuts at: 2554 3276 4848 2554
Size: 722 1572 3239
Fragments arranged by size:
3239 1572 722
ApaLI G'TGCA_C
Cuts at: 3590 4746 3590
Size: 1156 4377
ApoI r'AATT_y
Cuts at: 1493 2087 2842 3068 3220 5329 1493
Size: 594 755 226 152 2109 1697
Fragments arranged by size:
2109 1697 755 594 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4087 160
Size: 872 3055 1606
Fragments arranged by size:
3055 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5533
AvaI C'yCGr_G
Cuts at: 1870 1943 2259 2858 1870
Size: 73 316 599 4545
Fragments arranged by size:
4545 599 316 73
BamHI G'GATC_C
Cuts at: 2863 3230 2863
Size: 367 5166
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2456 2708 2854 4309 618
Size: 325 217 627 669 252 146 1455 1842
Fragments arranged by size:
1842 1455 669 627 325 252 217 146
BanII G_rGCy'C
Cuts at: 1391 2852 1391
Size: 1461 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4562
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3724 3758 1903
Size: 34 1787 34 3678
Fragments arranged by size:
3678 1787 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3425 4635 3425
Size: 1210 4323
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5533
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4144 136
Size: 107 122 71 631 605 2472 1525
Fragments arranged by size:
2472 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5528 5528
Size: 5533
BlpI GC'TnA_GC
Cuts at: 2468 2666 2950 2468
Size: 198 284 5051
Fragments arranged by size:
5051 284 198
Bme1580I G_kGCm'C
Cuts at: 1790 3594 4750 1790
Size: 1804 1156 2573
Fragments arranged by size:
2573 1804 1156
BmgBI CAC'GTC
Cuts at: 1318 2302 1318
Size: 984 4549
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2806 3264 4222
Size: 416 53 83 399 24 1389 458 958
Cuts at: 4222 442
Size: 1753
Fragments arranged by size:
1753 1389 958 458 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5533
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2504 2515 2549 4193 1239
Size: 1265 11 34 1644 2579
Fragments arranged by size:
2579 1644 1265 34 11
Bpu10I CC'TnA_GC
Cuts at: 2381 2381
Size: 5533
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3658 4523 4821 3658
Size: 865 298 4370
Fragments arranged by size:
4370 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2335 2795 4196 882
Size: 617 836 460 1401 2219
Fragments arranged by size:
2219 1401 836 617 460
BsaAI yAC'GTr
Cuts at: 493 1204 2204 2653 2752 3324 493
Size: 711 1000 449 99 572 2702
Fragments arranged by size:
2702 1000 711 572 449 99
BsaBI GATnn'nnATC
Cuts at: 5527 5527
Size: 5533
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2400 2450 3340 3722
Size: 53 83 186 936 867 50 890 382
Cuts at: 3722 4357 275
Size: 635 1451
Fragments arranged by size:
1451 936 890 867 635 382 186 83
53 50
BsaWI w'CCGG_w
Cuts at: 3966 4364 4638 4785 5196 3966
Size: 398 274 147 411 4303
Fragments arranged by size:
4303 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5308
Fragments arranged by size:
5308 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5533
BseYI C'CCAG_C
Cuts at: 2188 4736 5232 2188
Size: 2548 496 2489
Fragments arranged by size:
2548 2489 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2675 1163
Size: 573 939 4021
Fragments arranged by size:
4021 939 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2983 3744 4772 1540
Size: 275 186 982 761 1028 2301
Fragments arranged by size:
2301 1028 982 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2387 2571 2595 3594 3679 4750 2387
Size: 184 24 999 85 1071 3170
Fragments arranged by size:
3170 1071 999 184 85 24
BsmI GAATG_Cn'
Cuts at: 3044 3137 3044
Size: 93 5440
BsmBI CGTCTCn'nnnn_
Cuts at: 2391 2391
Size: 5533
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2387 2571 2595 2852 3594 3679 4750
Size: 399 597 184 24 257 742 85 1071
Cuts at: 4750 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 597 399 257 184 85
24
BspEI T'CCGG_A
Cuts at: 5196 5196
Size: 5533
BspHI T'CATG_A
Cuts at: 3422 5152 3422
Size: 1730 3803
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2241 2888 844
Size: 1397 647 3489
Fragments arranged by size:
3489 1397 647
BsrBI CCG'CTC
Cuts at: 1394 1823 3420 1394
Size: 429 1597 3507
Fragments arranged by size:
3507 1597 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4028 4202 65
Size: 1183 2780 174 1396
Fragments arranged by size:
2780 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4177 4364 1700
Size: 110 2367 187 2869
Fragments arranged by size:
2869 2367 187 110
BsrGI T'GTAC_A
Cuts at: 96 2349 96
Size: 2253 3280
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5533
BssSI C'ACGA_G
Cuts at: 3593 4605 3593
Size: 1012 4521
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2662 794
Size: 1868 3665
BstBI TT'CG_AA
Cuts at: 4337 4337
Size: 5533
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5533
BstXI CCAn_nnnn'nTGG
Cuts at: 2302 2584 2302
Size: 282 5251
BstYI r'GATC_y
Cuts at: 1972 2139 2863 3230 3615 3632 5084 5170 5182
Size: 167 724 367 385 17 1452 86 12
Cuts at: 5182 5528 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 724 385 367 346 167 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4981
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2557 487
Size: 683 56 1331 3463
Fragments arranged by size:
3463 1331 683 56
BtsI GCAGTG_nn'
Cuts at: 2822 3049 3843 3863 2822
Size: 227 794 20 4492
Fragments arranged by size:
4492 794 227 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5533
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5498
DraI TTT'AAA
Cuts at: 2837 2837
Size: 5533
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4540 809
Size: 507 3224 1802
Fragments arranged by size:
3224 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2625 2746 2980
Size: 54 1425 50 275 18 795 121 234
Cuts at: 2980 3869 5498 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 795 275 234 121 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2980 1537
Size: 275 1168 4090
Fragments arranged by size:
4090 1168 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2528 2598 3463 1353
Size: 1175 70 865 3423
Fragments arranged by size:
3423 1175 865 70
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4120 4494 4640 387
Size: 1064 138 2531 374 146 1280
Fragments arranged by size:
2531 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2342 2504 2515 2549 3596 4193 4980
Size: 393 710 162 11 34 1047 597 787
Cuts at: 4980 5248 1239
Size: 268 1524
Fragments arranged by size:
1524 1047 787 710 597 393 268 162
34 11
EcoNI CCTnn'n_nnAGG
Cuts at: 2324 2324
Size: 5533
EcoO109I rG'GnC_Cy
Cuts at: 2785 2785
Size: 5533
EcoRI G'AATT_C
Cuts at: 2087 2842 3220 2087
Size: 755 378 4400
Fragments arranged by size:
4400 755 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2524 2539 2524
Size: 15 5518
FspI TGC'GCA
Cuts at: 4039 4039
Size: 5533
HaeII r_GCGC'y
Cuts at: 1678 2555 4680 1678
Size: 877 2125 2531
Fragments arranged by size:
2531 2125 877
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2297 2329 4174 4206 4248
Size: 32 135 32 725 32 1845 32 42
Cuts at: 4248 4280 1373
Size: 32 2626
Fragments arranged by size:
2626 1845 725 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2877 3123 669
Size: 988 281 939 246 3079
Fragments arranged by size:
3079 988 939 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5533
HpaI GTT'AAC
Cuts at: 3123 3123
Size: 5533
KpnI G_GTAC'C
Cuts at: 2858 2858
Size: 5533
MfeI C'AATT_G
Cuts at: 3132 3132
Size: 5533
MluI A'CGCG_T
Cuts at: 2052 5409 2052
Size: 3357 2176
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4647 4831 1305
Size: 3342 184 2007
Fragments arranged by size:
3342 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3711
Fragments arranged by size:
3711 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2300 3491 3850 4009 518
Size: 648 1134 1191 359 159 2042
Fragments arranged by size:
2042 1191 1134 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2245 2614 2949 3626 4774 5019 1606
Size: 639 369 335 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 639 369 335 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4981
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5533
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5533
NotI GC'GGCC_GC
Cuts at: 2980 2980
Size: 5533
NspI r_CATG'y
Cuts at: 1132 1229 2891 4436 1132
Size: 97 1662 1545 2229
Fragments arranged by size:
2229 1662 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5533
PciI A'CATG_T
Cuts at: 4432 4432
Size: 5533
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5533
PfoI T'CCnGG_A
Cuts at: 2526 2583 2526
Size: 57 5476
PmeI GTTT'AAAC
Cuts at: 2837 2837
Size: 5533
PmlI CAC'GTG
Cuts at: 2204 2752 2204
Size: 548 4985
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3602 3634 5115 5147 3602
Size: 32 1481 32 3988
Fragments arranged by size:
3988 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2785 2785
Size: 5533
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5533
PsiI TTA'TAA
Cuts at: 3103 3103
Size: 5533
PstI C_TGCA'G
Cuts at: 830 2238 2829 2885 830
Size: 1408 591 56 3478
Fragments arranged by size:
3478 1408 591 56
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5533
PvuII CAG'CTG
Cuts at: 2245 2614 2245
Size: 369 5164
SalI G'TCGA_C
Cuts at: 1936 2875 1936
Size: 939 4594
SapI GCTCTTCn'nnn_
Cuts at: 2528 2528
Size: 5533
SbfI CC_TGCA'GG
Cuts at: 2885 2885
Size: 5533
ScaI AGT'ACT
Cuts at: 3781 5493 3781
Size: 1712 3821
SfcI C'TryA_G
Cuts at: 826 1045 2092 2234 2825 2881 4016 4697 4888
Size: 219 1047 142 591 56 1135 681 191
Cuts at: 4888 826
Size: 1471
Fragments arranged by size:
1471 1135 1047 681 591 219 191 142
56
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5533
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5533
SmaI CCC'GGG
Cuts at: 1872 2860 1872
Size: 988 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3637 4538 4800 820
Size: 197 926 1694 901 262 1553
Fragments arranged by size:
1694 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3324 493
Size: 2831 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5533
SphI G_CATG'C
Cuts at: 1132 2891 1132
Size: 1759 3774
SspI AAT'ATT
Cuts at: 5 52 3457 5
Size: 47 3405 2081
Fragments arranged by size:
3405 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5533
StyI C'CwwG_G
Cuts at: 513 1065 2559 2972 513
Size: 552 1494 413 3074
Fragments arranged by size:
3074 1494 552 413
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2297 3560 3577 3730 3915 4360 325
Size: 1255 717 1263 17 153 185 445 1498
Fragments arranged by size:
1498 1263 1255 717 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2349 3779 5491
Size: 274 80 33 51 1443 372 1430 1712
Cuts at: 5491 96
Size: 138
Fragments arranged by size:
1712 1443 1430 372 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5493 5525 5493
Size: 32 5501
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5533
XbaI T'CTAG_A
Cuts at: 2869 2869
Size: 5533
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2351 2351
Size: 5533
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5533
XmaI C'CCGG_G
Cuts at: 1870 2858 1870
Size: 988 4545
XmnI GAAnn'nnTTC
Cuts at: 3662 3662
Size: 5533
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2401 3341 276
Size: 53 83 186 936 867 940 2468
Fragments arranged by size:
2468 940 936 867 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SexAI SfiI SfoI SrfI SwaI