Restriction map for
pFN21AE0517
(Circular) (Six-base) MAPSORT of: pFN21AE0517.seq Check: 7324 from: 1 to: 5632
pFN21AE0517 5632 bp
With 182 enzymes: SgfI *
October 20, 2009 10:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2437 2450 2479 2437
Size: 13 29 5590
Fragments arranged by size:
5590 29 13
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3442 278
Size: 53 83 186 936 1906 2468
Fragments arranged by size:
2468 1906 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2975 1937
Size: 1038 4594
Acc65I G'GTAC_C
Cuts at: 2953 2953
Size: 5632
AclI AA'CG_TT
Cuts at: 1563 3759 4132 1563
Size: 2196 373 3063
Fragments arranged by size:
3063 2196 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2370 2903 3695 5079 5347 1632
Size: 738 533 792 1384 268 1917
Fragments arranged by size:
1917 1384 792 738 533 268
AfeI AGC'GCT
Cuts at: 2381 2381
Size: 5632
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5435
AflIII A'CryG_T
Cuts at: 1203 4531 5508 1203
Size: 3328 977 1327
Fragments arranged by size:
3328 1327 977
AgeI A'CCGG_T
Cuts at: 4463 4463
Size: 5632
AhdI GACnn_n'nnGTC
Cuts at: 4361 4361
Size: 5632
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5632
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2919 1184
Size: 1735 3897
AlwNI CAG_nnn'CTG
Cuts at: 2140 2161 2471 2806 2881 3375 4947 2140
Size: 21 310 335 75 494 1572 2825
Fragments arranged by size:
2825 1572 494 335 310 75 21
ApaI G_GGCC'C
Cuts at: 2403 2546 2403
Size: 143 5489
ApaLI G'TGCA_C
Cuts at: 3689 4845 3689
Size: 1156 4476
ApoI r'AATT_y
Cuts at: 1493 2941 3167 3319 5428 1493
Size: 1448 226 152 2109 1697
Fragments arranged by size:
2109 1697 1448 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4186 160
Size: 872 3154 1606
Fragments arranged by size:
3154 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5632
AvaI C'yCGr_G
Cuts at: 1870 1943 2104 2957 1870
Size: 73 161 853 4545
Fragments arranged by size:
4545 853 161 73
BamHI G'GATC_C
Cuts at: 2962 3329 2962
Size: 367 5265
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2552 2953 4408 618
Size: 325 217 627 765 401 1455 1842
Fragments arranged by size:
1842 1455 765 627 401 325 217
BanII G_rGCy'C
Cuts at: 1391 2403 2546 2951 1391
Size: 1012 143 405 4072
Fragments arranged by size:
4072 1012 405 143
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4661
BbvCI CC'TCA_GC
Cuts at: 2464 2638 2464
Size: 174 5458
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3823 3857 1903
Size: 34 50 34 1802 34 3678
Fragments arranged by size:
3678 1802 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3524 4734 3524
Size: 1210 4422
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5632
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4243 136
Size: 107 122 71 631 605 2571 1525
Fragments arranged by size:
2571 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5627 5627
Size: 5632
BlpI GC'TnA_GC
Cuts at: 2915 3049 2915
Size: 134 5498
Bme1580I G_kGCm'C
Cuts at: 1790 2403 2546 2557 2845 2860 3693 4849 1790
Size: 613 143 11 288 15 833 1156 2573
Fragments arranged by size:
2573 1156 833 613 288 143 15 11
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5632
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2549 3363 4321
Size: 416 53 83 399 24 1132 814 958
Cuts at: 4321 442
Size: 1753
Fragments arranged by size:
1753 1132 958 814 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5632
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2308 2308
Size: 5632
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2525 4292 1239
Size: 1286 1767 2579
Fragments arranged by size:
2579 1767 1286
Bpu10I CC'TnA_GC
Cuts at: 2464 2516 2638 2464
Size: 52 122 5458
Fragments arranged by size:
5458 122 52
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3757 4622 4920 3757
Size: 865 298 4469
Fragments arranged by size:
4469 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4295 882
Size: 617 2796 2219
Fragments arranged by size:
2796 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3423 493
Size: 711 2219 2702
Fragments arranged by size:
2702 2219 711
BsaBI GATnn'nnATC
Cuts at: 5626 5626
Size: 5632
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3439 3821 4456 275
Size: 53 83 186 936 1906 382 635 1451
Fragments arranged by size:
1906 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4065 4463 4737 4884 5295 4065
Size: 398 274 147 411 4402
Fragments arranged by size:
4402 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2180 2210 1178
Size: 30 165 30 777 30 4600
Fragments arranged by size:
4600 777 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2225 1186
Size: 1039 4593
BseYI C'CCAG_C
Cuts at: 2147 2491 2495 2583 2766 4835 5331 2147
Size: 344 4 88 183 2069 496 2448
Fragments arranged by size:
2448 2069 496 344 183 88 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2020 2915 1163
Size: 573 284 895 3880
Fragments arranged by size:
3880 895 573 284
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3082 3843 4871 1540
Size: 275 186 1081 761 1028 2301
Fragments arranged by size:
2301 1081 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3693 3778 4849 3693
Size: 85 1071 4476
Fragments arranged by size:
4476 1071 85
BsmI GAATG_Cn'
Cuts at: 3143 3236 3143
Size: 93 5539
BsmBI CGTCTCn'nnnn_
Cuts at: 2048 2048
Size: 5632
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2403 2546 2557 2845 2860 2951 3693
Size: 399 613 143 11 288 15 91 742
Cuts at: 3693 3778 4849 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 613 399 288 143 91
85 15 11
BspEI T'CCGG_A
Cuts at: 5295 5295
Size: 5632
BspHI T'CATG_A
Cuts at: 3521 5251 3521
Size: 1730 3902
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2437 2450 2479 2987 844
Size: 1593 13 29 508 3489
Fragments arranged by size:
3489 1593 508 29 13
BsrBI CCG'CTC
Cuts at: 1394 1823 2599 2839 3519 1394
Size: 429 776 240 680 3507
Fragments arranged by size:
3507 776 680 429 240
BsrDI GCAATG_nn'
Cuts at: 65 1248 4127 4301 65
Size: 1183 2879 174 1396
Fragments arranged by size:
2879 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2027 2358 2482 4276 4463 1700
Size: 110 217 331 124 1794 187 2869
Fragments arranged by size:
2869 1794 331 217 187 124 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5632
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5632
BssSI C'ACGA_G
Cuts at: 3692 4704 3692
Size: 1012 4620
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2806 794
Size: 2012 3620
BstBI TT'CG_AA
Cuts at: 4436 4436
Size: 5632
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5632
BstYI r'GATC_y
Cuts at: 1972 2962 3329 3714 3731 5183 5269 5281 5627
Size: 990 367 385 17 1452 86 12 346
Cuts at: 5627 1972
Size: 1977
Fragments arranged by size:
1977 1452 990 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2065 2621 513
Size: 552 1000 556 3524
Fragments arranged by size:
3524 1000 556 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4893
Fragments arranged by size:
4893 683 56
BtsI GCAGTG_nn'
Cuts at: 2014 3148 3942 3962 2014
Size: 1134 794 20 3684
Fragments arranged by size:
3684 1134 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5632
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2263 2298 529
Size: 35 1699 35 3863
Fragments arranged by size:
3863 1699 35 35
DraI TTT'AAA
Cuts at: 2936 2936
Size: 5632
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4639 809
Size: 507 3323 1802
Fragments arranged by size:
3323 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2124 2152 2624
Size: 54 1425 50 275 18 294 28 472
Cuts at: 2624 2928 3079 3968 5597 8
Size: 304 151 889 1629 43
Fragments arranged by size:
1629 1425 889 472 304 294 275 151
54 50 43 28 18
EagI C'GGCC_G
Cuts at: 1537 1812 3079 1537
Size: 275 1267 4090
Fragments arranged by size:
4090 1267 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2451 3562 1353
Size: 1098 1111 3423
Fragments arranged by size:
3423 1111 1098
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2011 4219 4593 4739 387
Size: 1064 138 422 2208 374 146 1280
Fragments arranged by size:
2208 1280 1064 422 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2370 2525 2903 3695 4292 5079 5347
Size: 393 738 155 378 792 597 787 268
Cuts at: 5347 1239
Size: 1524
Fragments arranged by size:
1524 792 787 738 597 393 378 268
155
EcoNI CCTnn'n_nnAGG
Cuts at: 2759 2759
Size: 5632
EcoO109I rG'GnC_Cy
Cuts at: 2158 2167 2399 2543 2158
Size: 9 232 144 5247
Fragments arranged by size:
5247 232 144 9
EcoRI G'AATT_C
Cuts at: 2941 3319 2941
Size: 378 5254
EcoRV GAT'ATC
Cuts at: 2246 2246
Size: 5632
FspI TGC'GCA
Cuts at: 4138 4138
Size: 5632
HaeII r_GCGC'y
Cuts at: 1678 2383 2583 2715 2773 4779 1678
Size: 705 200 132 58 2006 2531
Fragments arranged by size:
2531 2006 705 200 132 58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2159 2191 2664 2696 4273
Size: 32 135 32 587 32 473 32 1577
Cuts at: 4273 4305 4347 4379 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1577 587 473 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2976 3222 669
Size: 988 281 1038 246 3079
Fragments arranged by size:
3079 1038 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5632
HpaI GTT'AAC
Cuts at: 3222 3222
Size: 5632
KpnI G_GTAC'C
Cuts at: 2957 2957
Size: 5632
MfeI C'AATT_G
Cuts at: 3231 3231
Size: 5632
MluI A'CGCG_T
Cuts at: 5508 5508
Size: 5632
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4746 4930 1305
Size: 3441 184 2007
Fragments arranged by size:
3441 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2126 10
Size: 54 1425 343 294 3516
Fragments arranged by size:
3516 1425 343 294 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3590 3949 4108 518
Size: 648 2424 359 159 2042
Fragments arranged by size:
2424 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2067 2137 2151 2495 2776 2803 2914 3048
Size: 461 70 14 344 281 27 111 134
Cuts at: 3048 3725 4873 5118 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 461 344 281 245 134
111 70 27 14
NaeI GCC'GGC
Cuts at: 2029 2360 2484 2029
Size: 331 124 5177
Fragments arranged by size:
5177 331 124
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5080
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5632
NgoMIV G'CCGG_C
Cuts at: 2027 2358 2482 2027
Size: 331 124 5177
Fragments arranged by size:
5177 331 124
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5632
NotI GC'GGCC_GC
Cuts at: 3079 3079
Size: 5632
NspI r_CATG'y
Cuts at: 1132 1229 2894 2990 4535 1132
Size: 97 1665 96 1545 2229
Fragments arranged by size:
2229 1665 1545 97 96
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5632
PciI A'CATG_T
Cuts at: 4531 4531
Size: 5632
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2140 1108
Size: 1032 4600
PmeI GTTT'AAAC
Cuts at: 2936 2936
Size: 5632
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3701 3733 5214 5246 3701
Size: 32 1481 32 4087
Fragments arranged by size:
4087 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2167 2167
Size: 5632
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5632
PsiI TTA'TAA
Cuts at: 3202 3202
Size: 5632
PspOMI G'GGCC_C
Cuts at: 2399 2542 2399
Size: 143 5489
PstI C_TGCA'G
Cuts at: 830 2579 2781 2832 2984 830
Size: 1749 202 51 152 3478
Fragments arranged by size:
3478 1749 202 152 51
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5632
PvuII CAG'CTG
Cuts at: 2495 2776 2914 2495
Size: 281 138 5213
Fragments arranged by size:
5213 281 138
SacII CC_GC'GG
Cuts at: 2068 2068
Size: 5632
SalI G'TCGA_C
Cuts at: 1936 2974 1936
Size: 1038 4594
SanDI GG'GwC_CC
Cuts at: 2167 2167
Size: 5632
SbfI CC_TGCA'GG
Cuts at: 2984 2984
Size: 5632
ScaI AGT'ACT
Cuts at: 3880 5592 3880
Size: 1712 3920
SexAI A'CCwGG_T
Cuts at: 2700 2700
Size: 5632
SfcI C'TryA_G
Cuts at: 826 1045 2575 2777 2828 2980 4115 4796 4987
Size: 219 1530 202 51 152 1135 681 191
Cuts at: 4987 826
Size: 1471
Fragments arranged by size:
1530 1471 1135 681 219 202 191 152
51
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5632
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5632
SmaI CCC'GGG
Cuts at: 1872 2959 1872
Size: 1087 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3736 4637 4899 820
Size: 197 926 1793 901 262 1553
Fragments arranged by size:
1793 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3423 493
Size: 2930 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5632
SphI G_CATG'C
Cuts at: 1132 2990 1132
Size: 1858 3774
SspI AAT'ATT
Cuts at: 5 52 3556 5
Size: 47 3504 2081
Fragments arranged by size:
3504 2081 47
StuI AGG'CCT
Cuts at: 1517 2462 2783 1517
Size: 945 321 4366
Fragments arranged by size:
4366 945 321
StyI C'CwwG_G
Cuts at: 513 1065 2547 3071 513
Size: 552 1482 524 3074
Fragments arranged by size:
3074 1482 552 524
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2540 3659 3676 3829 4014 4459 325
Size: 1255 960 1119 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1119 960 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 3878 5590
Size: 274 80 33 51 1443 204 1697 1712
Cuts at: 5590 96
Size: 138
Fragments arranged by size:
1712 1697 1443 274 204 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2431 2463 5592 5624 2431
Size: 32 3129 32 2439
Fragments arranged by size:
3129 2439 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5632
XbaI T'CTAG_A
Cuts at: 2968 2968
Size: 5632
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5632
XmaI C'CCGG_G
Cuts at: 1870 2957 1870
Size: 1087 4545
XmnI GAAnn'nnTTC
Cuts at: 3761 3761
Size: 5632
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3440 276
Size: 53 83 186 936 1906 2468
Fragments arranged by size:
2468 1906 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacII SalI SanDI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI
BstZ17I Bsu36I DraIII EcoICRI FalI FseI FspAI KasI
NarI NruI NsiI PacI PfoI PmlI PspXI PsrI
RsrII SacI SapI SfiI SfoI SrfI SwaI XcmI