Restriction map for
pFN21AE0514
(Circular) (Six-base) MAPSORT of: pFN21AE0514.seq Check: 5430 from: 1 to: 5392
pFN21AE0514 5392 bp
With 182 enzymes: SgfI *
October 20, 2009 10:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3202 278
Size: 53 83 186 936 1666 2468
Fragments arranged by size:
2468 1666 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2735 1937
Size: 798 4594
Acc65I G'GTAC_C
Cuts at: 2713 2713
Size: 5392
AclI AA'CG_TT
Cuts at: 1563 3519 3892 1563
Size: 1956 373 3063
Fragments arranged by size:
3063 1956 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2174 2327 3455 4839 5107 1632
Size: 542 153 1128 1384 268 1917
Fragments arranged by size:
1917 1384 1128 542 268 153
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5195
AflIII A'CryG_T
Cuts at: 1203 4291 5268 1203
Size: 3088 977 1327
Fragments arranged by size:
3088 1327 977
AgeI A'CCGG_T
Cuts at: 4223 4223
Size: 5392
AhdI GACnn_n'nnGTC
Cuts at: 4121 4121
Size: 5392
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5392
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5392
AlwNI CAG_nnn'CTG
Cuts at: 2442 2509 3135 4707 2442
Size: 67 626 1572 3127
Fragments arranged by size:
3127 1572 626 67
ApaI G_GGCC'C
Cuts at: 2286 2475 2286
Size: 189 5203
ApaLI G'TGCA_C
Cuts at: 2381 3449 4605 2381
Size: 1068 1156 3168
Fragments arranged by size:
3168 1156 1068
ApoI r'AATT_y
Cuts at: 1493 2701 2927 3079 5188 1493
Size: 1208 226 152 2109 1697
Fragments arranged by size:
2109 1697 1208 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3946 160
Size: 872 2914 1606
Fragments arranged by size:
2914 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5392
AvaI C'yCGr_G
Cuts at: 1870 1943 2371 2717 1870
Size: 73 428 346 4545
Fragments arranged by size:
4545 428 346 73
BamHI G'GATC_C
Cuts at: 2722 3089 2722
Size: 367 5025
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2069 2713 4168 618
Size: 325 217 627 282 644 1455 1842
Fragments arranged by size:
1842 1455 644 627 325 282 217
BanII G_rGCy'C
Cuts at: 1391 2223 2286 2475 2711 1391
Size: 832 63 189 236 4072
Fragments arranged by size:
4072 832 236 189 63
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2452 928
Size: 971 553 3868
Fragments arranged by size:
3868 971 553
BbvCI CC'TCA_GC
Cuts at: 2489 2489
Size: 5392
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2362 2396 3583 3617 1903
Size: 34 425 34 1187 34 3678
Fragments arranged by size:
3678 1187 425 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3284 4494 3284
Size: 1210 4182
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5392
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4003 136
Size: 107 122 71 631 605 2331 1525
Fragments arranged by size:
2331 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2103 5387 2103
Size: 3284 2108
BlpI GC'TnA_GC
Cuts at: 2809 2809
Size: 5392
Bme1580I G_kGCm'C
Cuts at: 1790 2286 2385 2475 3453 4609 1790
Size: 496 99 90 978 1156 2573
Fragments arranged by size:
2573 1156 978 496 99 90
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5392
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2674 3123 4081
Size: 416 53 83 399 24 1257 449 958
Cuts at: 4081 442
Size: 1753
Fragments arranged by size:
1753 1257 958 449 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5392
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2545 2545
Size: 5392
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2120 2255 2656 4052 1239
Size: 881 135 401 1396 2579
Fragments arranged by size:
2579 1396 881 401 135
Bpu10I CC'TnA_GC
Cuts at: 2489 2489
Size: 5392
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2095 2198 3517 4382 4680 2095
Size: 103 1319 865 298 2807
Fragments arranged by size:
2807 1319 865 298 103
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4055 882
Size: 617 2556 2219
Fragments arranged by size:
2556 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2326 3183 493
Size: 711 1122 857 2702
Fragments arranged by size:
2702 1122 857 711
BsaBI GATnn'nnATC
Cuts at: 5386 5386
Size: 5392
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2457 3199 3581 4216
Size: 53 83 186 936 924 742 382 635
Cuts at: 4216 275
Size: 1451
Fragments arranged by size:
1451 936 924 742 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3825 4223 4497 4644 5055 3825
Size: 398 274 147 411 4162
Fragments arranged by size:
4162 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2129 2159 2387 2417 1178
Size: 30 165 30 726 30 228 30 4153
Fragments arranged by size:
4153 726 228 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2408 2648 1186
Size: 1222 240 3930
Fragments arranged by size:
3930 1222 240
BseYI C'CCAG_C
Cuts at: 2427 2509 2576 4595 5091 2427
Size: 82 67 2019 496 2728
Fragments arranged by size:
2728 2019 496 82 67
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4819
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2560 2842 3603 4631 1540
Size: 275 186 559 282 761 1028 2301
Fragments arranged by size:
2301 1028 761 559 282 275 186
BsiHKAI G_wGCw'C
Cuts at: 2256 2274 2385 3453 3538 4609 2256
Size: 18 111 1068 85 1071 3039
Fragments arranged by size:
3039 1071 1068 111 85 18
BsmI GAATG_Cn'
Cuts at: 2903 2996 2903
Size: 93 5299
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2223 2256 2274 2286 2385 2475 2711
Size: 399 433 33 18 12 99 90 236
Cuts at: 2711 3453 3538 4609 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 433 399 236 99 90
85 33 18 12
BspEI T'CCGG_A
Cuts at: 5055 5055
Size: 5392
BspHI T'CATG_A
Cuts at: 3281 5011 3281
Size: 1730 3662
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2747 844
Size: 1903 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3279 1394
Size: 429 1456 3507
Fragments arranged by size:
3507 1456 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3887 4061 65
Size: 1183 2639 174 1396
Fragments arranged by size:
2639 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4036 4223 1700
Size: 110 2226 187 2869
Fragments arranged by size:
2869 2226 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5392
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5392
BssSI C'ACGA_G
Cuts at: 3452 4464 3452
Size: 1012 4380
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5392
BstBI TT'CG_AA
Cuts at: 4196 4196
Size: 5392
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5392
BstYI r'GATC_y
Cuts at: 1972 2103 2196 2649 2667 2675 2722 3089 3474
Size: 131 93 453 18 8 47 367 385
Cuts at: 3474 3491 4943 5029 5041 5387 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 453 385 367 346 131 93
86 47 18 17 12 8
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3901
Fragments arranged by size:
3901 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2367 487
Size: 683 56 1141 3512
Fragments arranged by size:
3512 1141 683 56
BtsI GCAGTG_nn'
Cuts at: 2908 3702 3722 2908
Size: 794 20 4578
Fragments arranged by size:
4578 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5392
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5357
DraI TTT'AAA
Cuts at: 2551 2696 2551
Size: 145 5247
DraIII CAC_nnn'GTG
Cuts at: 2329 2329
Size: 5392
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4399 809
Size: 507 3083 1802
Fragments arranged by size:
3083 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2839 3728 5357
Size: 54 1425 50 275 18 1009 889 1629
Cuts at: 5357 8
Size: 43
Fragments arranged by size:
1629 1425 1009 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2839 1537
Size: 275 1027 4090
Fragments arranged by size:
4090 1027 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2607 3322 1353
Size: 1254 715 3423
Fragments arranged by size:
3423 1254 715
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3979 4353 4499 387
Size: 1064 138 2390 374 146 1280
Fragments arranged by size:
2390 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2120 2174 2255 2327 2656 3455 4052
Size: 393 488 54 81 72 329 799 597
Cuts at: 4052 4839 5107 1239
Size: 787 268 1524
Fragments arranged by size:
1524 799 787 597 488 393 329 268
81 72 54
EcoO109I rG'GnC_Cy
Cuts at: 2079 2283 2471 2472 2599 2079
Size: 204 188 1 127 4872
Fragments arranged by size:
4872 204 188 127 1
EcoRI G'AATT_C
Cuts at: 2701 3079 2701
Size: 378 5014
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2239 2239
Size: 5392
FspI TGC'GCA
Cuts at: 3898 3898
Size: 5392
HaeII r_GCGC'y
Cuts at: 1678 4539 1678
Size: 2861 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2303 2335 2387 2419 4033
Size: 32 135 32 731 32 52 32 1614
Cuts at: 4033 4065 4107 4139 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1614 731 135 52 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2736 2982 669
Size: 988 281 798 246 3079
Fragments arranged by size:
3079 988 798 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5392
HpaI GTT'AAC
Cuts at: 2982 2982
Size: 5392
KpnI G_GTAC'C
Cuts at: 2717 2717
Size: 5392
MfeI C'AATT_G
Cuts at: 2991 2991
Size: 5392
MluI A'CGCG_T
Cuts at: 5268 5268
Size: 5392
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2430 2556 4506 4690 1305
Size: 1125 126 1950 184 2007
Fragments arranged by size:
2007 1950 1125 184 126
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3570
Fragments arranged by size:
3570 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3350 3709 3868 518
Size: 648 2184 359 159 2042
Fragments arranged by size:
2184 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2143 2170 2261 2427 2808 3485 4633 4878
Size: 537 27 91 166 381 677 1148 245
Cuts at: 4878 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 537 381 245 166 91
27
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3901
Fragments arranged by size:
3901 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5392
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5392
NotI GC'GGCC_GC
Cuts at: 2839 2839
Size: 5392
NspI r_CATG'y
Cuts at: 1132 1229 2750 4295 1132
Size: 97 1521 1545 2229
Fragments arranged by size:
2229 1545 1521 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5392
PciI A'CATG_T
Cuts at: 4291 4291
Size: 5392
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2329 2509 1108
Size: 1221 180 3991
Fragments arranged by size:
3991 1221 180
PfoI T'CCnGG_A
Cuts at: 2243 2243
Size: 5392
PmeI GTTT'AAAC
Cuts at: 2696 2696
Size: 5392
PmlI CAC'GTG
Cuts at: 2326 2326
Size: 5392
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3461 3493 4974 5006 3461
Size: 32 1481 32 3847
Fragments arranged by size:
3847 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2079 2079
Size: 5392
PshAI GACnn'nnGTC
Cuts at: 1613 2456 1613
Size: 843 4549
PsiI TTA'TAA
Cuts at: 2962 2962
Size: 5392
PspOMI G'GGCC_C
Cuts at: 2282 2471 2282
Size: 189 5203
PstI C_TGCA'G
Cuts at: 830 2744 830
Size: 1914 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5392
PvuII CAG'CTG
Cuts at: 2143 2170 2143
Size: 27 5365
SalI G'TCGA_C
Cuts at: 1936 2734 1936
Size: 798 4594
SbfI CC_TGCA'GG
Cuts at: 2744 2744
Size: 5392
ScaI AGT'ACT
Cuts at: 3640 5352 3640
Size: 1712 3680
SfcI C'TryA_G
Cuts at: 826 1045 2740 3875 4556 4747 826
Size: 219 1695 1135 681 191 1471
Fragments arranged by size:
1695 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5392
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5392
SmaI CCC'GGG
Cuts at: 1872 2719 1872
Size: 847 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2074 2213 3496 4397 4659 820
Size: 197 926 131 139 1283 901 262 1553
Fragments arranged by size:
1553 1283 926 901 262 197 139 131
SnaBI TAC'GTA
Cuts at: 493 3183 493
Size: 2690 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5392
SphI G_CATG'C
Cuts at: 1132 2750 1132
Size: 1618 3774
SspI AAT'ATT
Cuts at: 5 52 3316 5
Size: 47 3264 2081
Fragments arranged by size:
3264 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5392
StyI C'CwwG_G
Cuts at: 513 1065 2004 2663 2831 513
Size: 552 939 659 168 3074
Fragments arranged by size:
3074 939 659 552 168
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3419 3436 3589 3774 4219 325
Size: 1255 1839 17 153 185 445 1498
Fragments arranged by size:
1839 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2190 3638 5350
Size: 274 80 33 51 1443 213 1448 1712
Cuts at: 5350 96
Size: 138
Fragments arranged by size:
1712 1448 1443 274 213 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5352 5384 5352
Size: 32 5360
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5392
XbaI T'CTAG_A
Cuts at: 2728 2728
Size: 5392
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5392
XmaI C'CCGG_G
Cuts at: 1870 2717 1870
Size: 847 4545
XmnI GAAnn'nnTTC
Cuts at: 3521 3521
Size: 5392
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3200 276
Size: 53 83 186 936 1666 2468
Fragments arranged by size:
2468 1666 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI