Restriction map for
pFN21AE0513
(Circular) (Six-base) MAPSORT of: pFN21AE0513.seq Check: 7917 from: 1 to: 5698
pFN21AE0513 5698 bp
With 182 enzymes: SgfI *
October 20, 2009 10:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2595 3508 278
Size: 53 83 186 936 1059 913 2468
Fragments arranged by size:
2468 1059 936 913 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2417 3041 1937
Size: 480 624 4594
Fragments arranged by size:
4594 624 480
Acc65I G'GTAC_C
Cuts at: 3019 3019
Size: 5698
AclI AA'CG_TT
Cuts at: 1563 2481 3825 4198 1563
Size: 918 1344 373 3063
Fragments arranged by size:
3063 1344 918 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2546 2870 2907 3761 5145 5413 1632
Size: 914 324 37 854 1384 268 1917
Fragments arranged by size:
1917 1384 914 854 324 268 37
AfeI AGC'GCT
Cuts at: 2450 2450
Size: 5698
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5501
AflIII A'CryG_T
Cuts at: 1203 2217 4597 5574 1203
Size: 1014 2380 977 1327
Fragments arranged by size:
2380 1327 1014 977
AgeI A'CCGG_T
Cuts at: 4529 4529
Size: 5698
AhdI GACnn_n'nnGTC
Cuts at: 2413 4427 2413
Size: 2014 3684
AleI CACnn'nnGTG
Cuts at: 1166 2327 1166
Size: 1161 4537
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5698
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2000 2032 2204 2236 2000
Size: 32 172 32 5462
Fragments arranged by size:
5462 172 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2257 2674 2704 3441 5013 2257
Size: 417 30 737 1572 2942
Fragments arranged by size:
2942 1572 737 417 30
ApaLI G'TGCA_C
Cuts at: 3755 4911 3755
Size: 1156 4542
ApoI r'AATT_y
Cuts at: 1493 2129 2783 3007 3233 3385 5494 1493
Size: 636 654 224 226 152 2109 1697
Fragments arranged by size:
2109 1697 654 636 226 224 152
AseI AT'TA_AT
Cuts at: 160 1032 4252 160
Size: 872 3220 1606
Fragments arranged by size:
3220 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5698
AvaI C'yCGr_G
Cuts at: 1870 1943 2183 3023 1870
Size: 73 240 840 4545
Fragments arranged by size:
4545 840 240 73
BamHI G'GATC_C
Cuts at: 3028 3395 3028
Size: 367 5331
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3019 4474 618
Size: 325 217 627 1232 1455 1842
Fragments arranged by size:
1842 1455 1232 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2058 2076 2232 2992 3017 1391
Size: 667 18 156 760 25 4072
Fragments arranged by size:
4072 760 667 156 25 18
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4727
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3889 3923 1903
Size: 34 1952 34 3678
Fragments arranged by size:
3678 1952 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3590 4800 3590
Size: 1210 4488
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5698
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4309 136
Size: 107 122 71 631 605 2637 1525
Fragments arranged by size:
2637 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5693 5693
Size: 5698
BlpI GC'TnA_GC
Cuts at: 2867 3115 2867
Size: 248 5450
Bme1580I G_kGCm'C
Cuts at: 1790 3759 4915 1790
Size: 1969 1156 2573
Fragments arranged by size:
2573 1969 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5698
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2707 3429 4387
Size: 416 53 83 399 24 1290 722 958
Cuts at: 4387 442
Size: 1753
Fragments arranged by size:
1753 1290 958 722 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5698
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2092 2092
Size: 5698
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2228 2354 2381 2861 4358 1239
Size: 989 126 27 480 1497 2579
Fragments arranged by size:
2579 1497 989 480 126 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2252 3823 4688 4986 2252
Size: 1571 865 298 2964
Fragments arranged by size:
2964 1571 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4361 882
Size: 617 2862 2219
Fragments arranged by size:
2862 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3489 493
Size: 711 2285 2702
Fragments arranged by size:
2702 2285 711
BsaBI GATnn'nnATC
Cuts at: 5692 5692
Size: 5698
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2592 2978 3505 3887
Size: 53 83 186 936 1059 386 527 382
Cuts at: 3887 4522 275
Size: 635 1451
Fragments arranged by size:
1451 1059 936 635 527 386 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2346 2620 4131 4529 4803 4950 5361 2346
Size: 274 1511 398 274 147 411 2683
Fragments arranged by size:
2683 1511 411 398 274 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2000 2006 2030 2036 2204
Size: 30 165 30 597 6 24 6 168
Cuts at: 2204 2234 1178
Size: 30 4642
Fragments arranged by size:
4642 597 168 165 30 30 30 24
6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2081 2084 2225 2378 1186
Size: 895 3 141 153 4506
Fragments arranged by size:
4506 895 153 141 3
BseYI C'CCAG_C
Cuts at: 2059 4901 5397 2059
Size: 2842 496 2360
Fragments arranged by size:
2842 2360 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2408 2717 2756 2804 1163
Size: 573 672 309 39 48 4057
Fragments arranged by size:
4057 672 573 309 48 39
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3148 3909 4937 1540
Size: 275 186 1147 761 1028 2301
Fragments arranged by size:
2301 1147 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2076 2190 2513 2918 2931 3759 3844 4915 2076
Size: 114 323 405 13 828 85 1071 2859
Fragments arranged by size:
2859 1071 828 405 323 114 85 13
BsmI GAATG_Cn'
Cuts at: 3209 3302 3209
Size: 93 5605
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2058 2076 2190 2232 2513 2918 2931
Size: 399 268 18 114 42 281 405 13
Cuts at: 2931 2992 3017 3759 3844 4915 1391
Size: 61 25 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 405 399 281 268 114
85 61 42 25 18 13
BspEI T'CCGG_A
Cuts at: 2620 5361 2620
Size: 2741 2957
BspHI T'CATG_A
Cuts at: 3587 5317 3587
Size: 1730 3968
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2037 2109 3053 844
Size: 1193 72 944 3489
Fragments arranged by size:
3489 1193 944 72
BsrBI CCG'CTC
Cuts at: 1394 1823 3585 1394
Size: 429 1762 3507
Fragments arranged by size:
3507 1762 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4193 4367 65
Size: 1183 2945 174 1396
Fragments arranged by size:
2945 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4342 4529 1700
Size: 110 2532 187 2869
Fragments arranged by size:
2869 2532 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5698
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5698
BssSI C'ACGA_G
Cuts at: 3758 4770 3758
Size: 1012 4686
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5698
BstBI TT'CG_AA
Cuts at: 4502 4502
Size: 5698
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5698
BstXI CCAn_nnnn'nTGG
Cuts at: 2050 2050
Size: 5698
BstYI r'GATC_y
Cuts at: 1972 3028 3395 3780 3797 5249 5335 5347 5693
Size: 1056 367 385 17 1452 86 12 346
Cuts at: 5693 1972
Size: 1977
Fragments arranged by size:
1977 1452 1056 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2983 513
Size: 552 939 979 3228
Fragments arranged by size:
3228 979 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2272 487
Size: 683 56 1046 3913
Fragments arranged by size:
3913 1046 683 56
BtsI GCAGTG_nn'
Cuts at: 2643 3214 4008 4028 2643
Size: 571 794 20 4313
Fragments arranged by size:
4313 794 571 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5698
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5663
DraI TTT'AAA
Cuts at: 3002 3002
Size: 5698
DraIII CAC_nnn'GTG
Cuts at: 2194 2194
Size: 5698
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4705 809
Size: 507 3389 1802
Fragments arranged by size:
3389 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2442 3145 4034
Size: 54 1425 50 275 18 612 703 889
Cuts at: 4034 5663 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 703 612 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3145 1537
Size: 275 1333 4090
Fragments arranged by size:
4090 1333 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2181 3628 1353
Size: 828 1447 3423
Fragments arranged by size:
3423 1447 828
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4285 4659 4805 387
Size: 1064 138 2696 374 146 1280
Fragments arranged by size:
2696 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2074 2074
Size: 5698
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2228 2354 2381 2546 2861 2870 2907
Size: 393 596 126 27 165 315 9 37
Cuts at: 2907 3761 4358 5145 5413 1239
Size: 854 597 787 268 1524
Fragments arranged by size:
1524 854 787 597 596 393 315 268
165 126 37 27 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2210 2210
Size: 5698
EcoO109I rG'GnC_Cy
Cuts at: 2122 2407 2860 2122
Size: 285 453 4960
Fragments arranged by size:
4960 453 285
EcoRI G'AATT_C
Cuts at: 2783 3007 3385 2783
Size: 224 378 5096
Fragments arranged by size:
5096 378 224
EcoRV GAT'ATC
Cuts at: 2893 2893
Size: 5698
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2611 2611
Size: 5698
FspI TGC'GCA
Cuts at: 4204 4204
Size: 5698
HaeII r_GCGC'y
Cuts at: 1678 2267 2452 2840 4845 1678
Size: 589 185 388 2005 2531
Fragments arranged by size:
2531 2005 589 388 185
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2399 2431 4339 4371 4413
Size: 32 135 32 827 32 1908 32 42
Cuts at: 4413 4445 1373
Size: 32 2626
Fragments arranged by size:
2626 1908 827 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2665 3042 3288 669
Size: 988 281 727 377 246 3079
Fragments arranged by size:
3079 988 727 377 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5698
HpaI GTT'AAC
Cuts at: 3288 3288
Size: 5698
KpnI G_GTAC'C
Cuts at: 3023 3023
Size: 5698
MfeI C'AATT_G
Cuts at: 3297 3297
Size: 5698
MluI A'CGCG_T
Cuts at: 5574 5574
Size: 5698
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4812 4996 1305
Size: 3507 184 2007
Fragments arranged by size:
3507 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2444 10
Size: 54 1425 343 612 3264
Fragments arranged by size:
3264 1425 612 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2327 3656 4015 4174 518
Size: 648 1161 1329 359 159 2042
Fragments arranged by size:
2042 1329 1161 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2063 2359 2871 3114 3791 4939 5184 1606
Size: 457 296 512 243 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 512 457 296 245 243
NcoI C'CATG_G
Cuts at: 513 1065 2004 2983 513
Size: 552 939 979 3228
Fragments arranged by size:
3228 979 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5698
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5698
NotI GC'GGCC_GC
Cuts at: 3145 3145
Size: 5698
NspI r_CATG'y
Cuts at: 1132 1229 2221 3056 4601 1132
Size: 97 992 835 1545 2229
Fragments arranged by size:
2229 1545 992 835 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5698
PciI A'CATG_T
Cuts at: 2217 4597 2217
Size: 2380 3318
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2257 2674 1108
Size: 1149 417 4132
Fragments arranged by size:
4132 1149 417
PfoI T'CCnGG_A
Cuts at: 2114 2114
Size: 5698
PmeI GTTT'AAAC
Cuts at: 3002 3002
Size: 5698
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2000 2032 2204 2236 3767 3799 5280 5312 2000
Size: 32 172 32 1531 32 1481 32 2386
Fragments arranged by size:
2386 1531 1481 172 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2122 2407 2122
Size: 285 5413
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5698
PsiI TTA'TAA
Cuts at: 3268 3268
Size: 5698
PstI C_TGCA'G
Cuts at: 830 2037 2145 2358 2812 3050 830
Size: 1207 108 213 454 238 3478
Fragments arranged by size:
3478 1207 454 238 213 108
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5698
PvuII CAG'CTG
Cuts at: 2359 2871 2359
Size: 512 5186
SacI G_AGCT'C
Cuts at: 2076 2076
Size: 5698
SalI G'TCGA_C
Cuts at: 1936 3040 1936
Size: 1104 4594
SbfI CC_TGCA'GG
Cuts at: 3050 3050
Size: 5698
ScaI AGT'ACT
Cuts at: 3946 5658 3946
Size: 1712 3986
SfcI C'TryA_G
Cuts at: 826 1045 2033 2141 2354 2808 3046 4181 4862
Size: 219 988 108 213 454 238 1135 681
Cuts at: 4862 5053 826
Size: 191 1471
Fragments arranged by size:
1471 1135 988 681 454 238 219 213
191 108
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5698
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5698
SmaI CCC'GGG
Cuts at: 1872 3025 1872
Size: 1153 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2267 3802 4703 4965 820
Size: 197 926 324 1535 901 262 1553
Fragments arranged by size:
1553 1535 926 901 324 262 197
SnaBI TAC'GTA
Cuts at: 493 3489 493
Size: 2996 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5698
SphI G_CATG'C
Cuts at: 1132 3056 1132
Size: 1924 3774
SspI AAT'ATT
Cuts at: 5 52 3622 5
Size: 47 3570 2081
Fragments arranged by size:
3570 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5698
StyI C'CwwG_G
Cuts at: 513 1065 2004 2043 2983 3137 513
Size: 552 939 39 940 154 3074
Fragments arranged by size:
3074 940 939 552 154 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2122 2726 3725 3742 3895 4080 4525
Size: 1255 542 604 999 17 153 185 445
Cuts at: 4525 325
Size: 1498
Fragments arranged by size:
1498 1255 999 604 542 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3944 5656 96
Size: 274 80 33 51 1443 1967 1712 138
Fragments arranged by size:
1967 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5658 5690 5658
Size: 32 5666
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5698
XbaI T'CTAG_A
Cuts at: 3034 3034
Size: 5698
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2383 2485 2720 2383
Size: 102 235 5361
Fragments arranged by size:
5361 235 102
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5698
XmaI C'CCGG_G
Cuts at: 1870 3023 1870
Size: 1153 4545
XmnI GAAnn'nnTTC
Cuts at: 3827 3827
Size: 5698
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2593 3506 276
Size: 53 83 186 936 1059 913 2468
Fragments arranged by size:
2468 1059 936 913 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI
PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI