Restriction map for
pFN21AE0512
(Circular) (Six-base) MAPSORT of: pFN21AE0512.seq Check: 407 from: 1 to: 5560
pFN21AE0512 5560 bp
With 182 enzymes: SgfI *
October 20, 2009 10:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3370 278
Size: 53 83 186 936 1834 2468
Fragments arranged by size:
2468 1834 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2040 2903 1937
Size: 103 863 4594
Fragments arranged by size:
4594 863 103
Acc65I G'GTAC_C
Cuts at: 2881 2881
Size: 5560
AclI AA'CG_TT
Cuts at: 1563 3687 4060 1563
Size: 2124 373 3063
Fragments arranged by size:
3063 2124 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3623 5007 5275 1632
Size: 1991 1384 268 1917
Fragments arranged by size:
1991 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5363
AflIII A'CryG_T
Cuts at: 1203 2097 2595 4459 5436 1203
Size: 894 498 1864 977 1327
Fragments arranged by size:
1864 1327 977 894 498
AgeI A'CCGG_T
Cuts at: 4391 4391
Size: 5560
AhdI GACnn_n'nnGTC
Cuts at: 4289 4289
Size: 5560
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5560
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5560
AlwNI CAG_nnn'CTG
Cuts at: 2704 2722 3303 4875 2704
Size: 18 581 1572 3389
Fragments arranged by size:
3389 1572 581 18
ApaI G_GGCC'C
Cuts at: 2024 2024
Size: 5560
ApaLI G'TGCA_C
Cuts at: 3617 4773 3617
Size: 1156 4404
ApoI r'AATT_y
Cuts at: 1493 2869 3095 3247 5356 1493
Size: 1376 226 152 2109 1697
Fragments arranged by size:
2109 1697 1376 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4114 160
Size: 872 3082 1606
Fragments arranged by size:
3082 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5560
AvaI C'yCGr_G
Cuts at: 1870 1943 2531 2885 1870
Size: 73 588 354 4545
Fragments arranged by size:
4545 588 354 73
BamHI G'GATC_C
Cuts at: 2509 2890 3257 2509
Size: 381 367 4812
Fragments arranged by size:
4812 381 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2881 4336 618
Size: 325 217 627 1094 1455 1842
Fragments arranged by size:
1842 1455 1094 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2024 2367 2409 2532 2559 2826 2879 1391
Size: 633 343 42 123 27 267 53 4072
Fragments arranged by size:
4072 633 343 267 123 53 42 27
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2116 928
Size: 971 217 4372
Fragments arranged by size:
4372 971 217
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2512 2546 3751 3785 1903
Size: 34 575 34 1205 34 3678
Fragments arranged by size:
3678 1205 575 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3452 4662 3452
Size: 1210 4350
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5560
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4171 136
Size: 107 122 71 631 605 2499 1525
Fragments arranged by size:
2499 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5555 5555
Size: 5560
BlpI GC'TnA_GC
Cuts at: 2977 2977
Size: 5560
Bme1580I G_kGCm'C
Cuts at: 1790 2024 2780 3621 4777 1790
Size: 234 756 841 1156 2573
Fragments arranged by size:
2573 1156 841 756 234
BmgBI CAC'GTC
Cuts at: 1318 2100 2794 1318
Size: 782 694 4084
Fragments arranged by size:
4084 782 694
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2182 2404 3291
Size: 416 53 83 399 24 765 222 887
Cuts at: 3291 4249 442
Size: 958 1753
Fragments arranged by size:
1753 958 887 765 416 399 222 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5560
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2489 4220 1239
Size: 1250 1731 2579
Fragments arranged by size:
2579 1731 1250
Bpu10I CC'TnA_GC
Cuts at: 2852 2852
Size: 5560
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3685 4550 4848 3685
Size: 865 298 4397
Fragments arranged by size:
4397 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4223 882
Size: 617 2724 2219
Fragments arranged by size:
2724 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3351 493
Size: 711 2147 2702
Fragments arranged by size:
2702 2147 711
BsaBI GATnn'nnATC
Cuts at: 5554 5554
Size: 5560
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3367 3749 4384 275
Size: 53 83 186 936 1834 382 635 1451
Fragments arranged by size:
1834 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2088 2322 2655 3993 4391 4665 4812 5223 2088
Size: 234 333 1338 398 274 147 411 2425
Fragments arranged by size:
2425 1338 411 398 333 274 234 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2396 2426 1178
Size: 30 165 30 993 30 4312
Fragments arranged by size:
4312 993 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5560
BseYI C'CCAG_C
Cuts at: 2856 4763 5259 2856
Size: 1907 496 3157
Fragments arranged by size:
3157 1907 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2354 2728 1163
Size: 573 618 374 3995
Fragments arranged by size:
3995 618 573 374
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3010 3771 4799 1540
Size: 275 186 1009 761 1028 2301
Fragments arranged by size:
2301 1028 1009 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2532 3621 3706 4777 2532
Size: 1089 85 1071 3315
Fragments arranged by size:
3315 1089 1071 85
BsmI GAATG_Cn'
Cuts at: 3071 3164 3071
Size: 93 5467
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2024 2367 2409 2532 2559 2780 2826
Size: 399 234 343 42 123 27 221 46
Cuts at: 2826 2879 3621 3706 4777 1391
Size: 53 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 343 234 221 123
85 53 46 42 27
BspEI T'CCGG_A
Cuts at: 2088 2322 5223 2088
Size: 234 2901 2425
Fragments arranged by size:
2901 2425 234
BspHI T'CATG_A
Cuts at: 3449 5179 3449
Size: 1730 3830
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2247 2793 2915 844
Size: 1403 546 122 3489
Fragments arranged by size:
3489 1403 546 122
BsrBI CCG'CTC
Cuts at: 1394 1823 3447 1394
Size: 429 1624 3507
Fragments arranged by size:
3507 1624 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4055 4229 65
Size: 1183 2807 174 1396
Fragments arranged by size:
2807 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4204 4391 1700
Size: 110 2394 187 2869
Fragments arranged by size:
2869 2394 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5560
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5560
BssSI C'ACGA_G
Cuts at: 3620 4632 3620
Size: 1012 4548
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5560
BstBI TT'CG_AA
Cuts at: 4364 4364
Size: 5560
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5560
BstYI r'GATC_y
Cuts at: 1972 2509 2890 3257 3642 3659 5111 5197 5209
Size: 537 381 367 385 17 1452 86 12
Cuts at: 5209 5555 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 537 385 381 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2637 2637
Size: 5560
BtgI C'CryG_G
Cuts at: 513 1065 2232 2463 513
Size: 552 1167 231 3610
Fragments arranged by size:
3610 1167 552 231
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4821
Fragments arranged by size:
4821 683 56
BtsI GCAGTG_nn'
Cuts at: 2627 3076 3870 3890 2627
Size: 449 794 20 4297
Fragments arranged by size:
4297 794 449 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5560
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5525
DraI TTT'AAA
Cuts at: 2864 2864
Size: 5560
DraIII CAC_nnn'GTG
Cuts at: 2775 2775
Size: 5560
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4567 809
Size: 507 3251 1802
Fragments arranged by size:
3251 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2067 2724 3007
Size: 54 1425 50 275 18 237 657 283
Cuts at: 3007 3896 5525 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 657 283 275 237 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3007 1537
Size: 275 1195 4090
Fragments arranged by size:
4090 1195 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3490 1353
Size: 2137 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4147 4521 4667 387
Size: 1064 138 2558 374 146 1280
Fragments arranged by size:
2558 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2530 2530
Size: 5560
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2489 3623 4220 5007 5275 1239
Size: 393 857 1134 597 787 268 1524
Fragments arranged by size:
1524 1134 857 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2381 2381
Size: 5560
EcoO109I rG'GnC_Cy
Cuts at: 2105 2386 2566 2719 2105
Size: 281 180 153 4946
Fragments arranged by size:
4946 281 180 153
EcoRI G'AATT_C
Cuts at: 2869 3247 2869
Size: 378 5182
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2101 2101
Size: 5560
FspI TGC'GCA
Cuts at: 4066 4066
Size: 5560
HaeII r_GCGC'y
Cuts at: 1678 4707 1678
Size: 3029 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4201 4233 4275 4307 1373
Size: 32 135 32 2629 32 42 32 2626
Fragments arranged by size:
2629 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2904 3150 669
Size: 988 281 966 246 3079
Fragments arranged by size:
3079 988 966 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5560
HpaI GTT'AAC
Cuts at: 3150 3150
Size: 5560
KpnI G_GTAC'C
Cuts at: 2885 2885
Size: 5560
MfeI C'AATT_G
Cuts at: 3159 3159
Size: 5560
MluI A'CGCG_T
Cuts at: 5436 5436
Size: 5560
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2244 2422 4674 4858 1305
Size: 939 178 2252 184 2007
Fragments arranged by size:
2252 2007 939 184 178
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2069 2726 10
Size: 54 1425 343 237 657 2844
Fragments arranged by size:
2844 1425 657 343 237 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3518 3877 4036 518
Size: 648 843 1509 359 159 2042
Fragments arranged by size:
2042 1509 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2312 2704 2976 3653 4801 5046 1606
Size: 706 392 272 677 1148 245 2120
Fragments arranged by size:
2120 1148 706 677 392 272 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5008
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5560
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5560
NotI GC'GGCC_GC
Cuts at: 3007 3007
Size: 5560
NspI r_CATG'y
Cuts at: 1132 1229 2186 2599 2918 4463 1132
Size: 97 957 413 319 1545 2229
Fragments arranged by size:
2229 1545 957 413 319 97
PasI CC'CwG_GG
Cuts at: 1348 2736 1348
Size: 1388 4172
PciI A'CATG_T
Cuts at: 2595 4459 2595
Size: 1864 3696
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2161 1108
Size: 1053 4507
PfoI T'CCnGG_A
Cuts at: 2112 2487 2505 2112
Size: 375 18 5167
Fragments arranged by size:
5167 375 18
PmeI GTTT'AAAC
Cuts at: 2864 2864
Size: 5560
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3629 3661 5142 5174 3629
Size: 32 1481 32 4015
Fragments arranged by size:
4015 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2105 2386 2566 2719 2105
Size: 281 180 153 4946
Fragments arranged by size:
4946 281 180 153
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5560
PsiI TTA'TAA
Cuts at: 3130 3130
Size: 5560
PspOMI G'GGCC_C
Cuts at: 2020 2020
Size: 5560
PstI C_TGCA'G
Cuts at: 830 2912 830
Size: 2082 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5560
PvuII CAG'CTG
Cuts at: 2704 2704
Size: 5560
SacI G_AGCT'C
Cuts at: 2532 2532
Size: 5560
SalI G'TCGA_C
Cuts at: 1936 2902 1936
Size: 966 4594
SbfI CC_TGCA'GG
Cuts at: 2912 2912
Size: 5560
ScaI AGT'ACT
Cuts at: 2837 3808 5520 2837
Size: 971 1712 2877
Fragments arranged by size:
2877 1712 971
SexAI A'CCwGG_T
Cuts at: 2601 2601
Size: 5560
SfcI C'TryA_G
Cuts at: 826 1045 2908 4043 4724 4915 826
Size: 219 1863 1135 681 191 1471
Fragments arranged by size:
1863 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5560
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5560
SmaI CCC'GGG
Cuts at: 1872 2887 1872
Size: 1015 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3664 4565 4827 820
Size: 197 926 1721 901 262 1553
Fragments arranged by size:
1721 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3351 493
Size: 2858 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5560
SphI G_CATG'C
Cuts at: 1132 2186 2918 1132
Size: 1054 732 3774
Fragments arranged by size:
3774 1054 732
SspI AAT'ATT
Cuts at: 5 52 3484 5
Size: 47 3432 2081
Fragments arranged by size:
3432 2081 47
StuI AGG'CCT
Cuts at: 1517 2459 1517
Size: 942 4618
StyI C'CwwG_G
Cuts at: 513 1065 2999 513
Size: 552 1934 3074
Fragments arranged by size:
3074 1934 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2349 2728 3587 3604 3757 3942 4387
Size: 1255 769 379 859 17 153 185 445
Cuts at: 4387 325
Size: 1498
Fragments arranged by size:
1498 1255 859 769 445 379 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2592 2835 3806
Size: 274 80 33 51 1443 615 243 971
Cuts at: 3806 5518 96
Size: 1712 138
Fragments arranged by size:
1712 1443 971 615 274 243 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2089 2121 2396 2428 5520 5552 2089
Size: 32 275 32 3092 32 2097
Fragments arranged by size:
3092 2097 275 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2120 2168 2564 1316
Size: 804 48 396 4312
Fragments arranged by size:
4312 804 396 48
XbaI T'CTAG_A
Cuts at: 2896 2896
Size: 5560
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2343 2343
Size: 5560
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5560
XmaI C'CCGG_G
Cuts at: 1870 2885 1870
Size: 1015 4545
XmnI GAAnn'nnTTC
Cuts at: 3689 3689
Size: 5560
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3368 276
Size: 53 83 186 936 1834 2468
Fragments arranged by size:
2468 1834 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI RsrII SacII SanDI SapI SfiI
SfoI SrfI SwaI