Restriction map for
pFN21AE0508
(Circular) (Six-base) MAPSORT of: pFN21AE0508.seq Check: 1059 from: 1 to: 5209
pFN21AE0508 5209 bp
With 182 enzymes: SgfI *
October 20, 2009 10:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3019 278
Size: 53 83 186 936 1483 2468
Fragments arranged by size:
2468 1483 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2552 1937
Size: 615 4594
Acc65I G'GTAC_C
Cuts at: 2530 2530
Size: 5209
AclI AA'CG_TT
Cuts at: 1563 3336 3709 1563
Size: 1773 373 3063
Fragments arranged by size:
3063 1773 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2320 2424 3272 4656 4924 1632
Size: 688 104 848 1384 268 1917
Fragments arranged by size:
1917 1384 848 688 268 104
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5012
AflIII A'CryG_T
Cuts at: 1203 2040 4108 5085 1203
Size: 837 2068 977 1327
Fragments arranged by size:
2068 1327 977 837
AgeI A'CCGG_T
Cuts at: 4040 4040
Size: 5209
AhdI GACnn_n'nnGTC
Cuts at: 3938 3938
Size: 5209
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5209
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5209
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2273 2305 2273
Size: 32 5177
AlwNI CAG_nnn'CTG
Cuts at: 2213 2359 2952 4524 2213
Size: 146 593 1572 2898
Fragments arranged by size:
2898 1572 593 146
ApaLI G'TGCA_C
Cuts at: 2132 3266 4422 2132
Size: 1134 1156 2919
Fragments arranged by size:
2919 1156 1134
ApoI r'AATT_y
Cuts at: 1493 2518 2744 2896 5005 1493
Size: 1025 226 152 2109 1697
Fragments arranged by size:
2109 1697 1025 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3763 160
Size: 872 2731 1606
Fragments arranged by size:
2731 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5209
AvaI C'yCGr_G
Cuts at: 1870 1943 2182 2534 1870
Size: 73 239 352 4545
Fragments arranged by size:
4545 352 239 73
BamHI G'GATC_C
Cuts at: 2539 2906 2539
Size: 367 4842
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2530 3985 618
Size: 325 217 627 743 1455 1842
Fragments arranged by size:
1842 1455 743 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2483 2528 1391
Size: 1092 45 4072
Fragments arranged by size:
4072 1092 45
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4238
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3400 3434 1903
Size: 34 1463 34 3678
Fragments arranged by size:
3678 1463 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3101 4311 3101
Size: 1210 3999
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5209
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3820 136
Size: 107 122 71 631 605 2148 1525
Fragments arranged by size:
2148 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5204 5204
Size: 5209
BlpI GC'TnA_GC
Cuts at: 2075 2626 2075
Size: 551 4658
Bme1580I G_kGCm'C
Cuts at: 1790 2136 3270 4426 1790
Size: 346 1134 1156 2573
Fragments arranged by size:
2573 1156 1134 346
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5209
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2373 2940 3898
Size: 416 53 83 399 24 956 567 958
Cuts at: 3898 442
Size: 1753
Fragments arranged by size:
1753 958 956 567 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5209
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2284 2284
Size: 5209
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2155 2190 2279 2454 3869 1239
Size: 916 35 89 175 1415 2579
Fragments arranged by size:
2579 1415 916 175 89 35
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3334 4199 4497 3334
Size: 865 298 4046
Fragments arranged by size:
4046 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3872 882
Size: 617 2373 2219
Fragments arranged by size:
2373 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2041 3000 493
Size: 711 837 959 2702
Fragments arranged by size:
2702 959 837 711
BsaBI GATnn'nnATC
Cuts at: 5203 5203
Size: 5209
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3016 3398 4033 275
Size: 53 83 186 936 1483 382 635 1451
Fragments arranged by size:
1483 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2026 3642 4040 4314 4461 4872 2026
Size: 1616 398 274 147 411 2363
Fragments arranged by size:
2363 1616 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2255 2273 2285 2303 1178
Size: 30 165 30 852 18 12 18 4084
Fragments arranged by size:
4084 852 165 30 30 18 18 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2260 1186
Size: 1074 4135
BseYI C'CCAG_C
Cuts at: 2089 4412 4908 2089
Size: 2323 496 2390
Fragments arranged by size:
2390 2323 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4636
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2659 3420 4448 1540
Size: 275 186 658 761 1028 2301
Fragments arranged by size:
2301 1028 761 658 275 186
BsiHKAI G_wGCw'C
Cuts at: 2136 2483 3270 3355 4426 2136
Size: 347 787 85 1071 2919
Fragments arranged by size:
2919 1071 787 347 85
BsmI GAATG_Cn'
Cuts at: 2720 2813 2720
Size: 93 5116
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2136 2483 2528 3270 3355 4426 1391
Size: 399 346 347 45 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 347 346 85 45
BspEI T'CCGG_A
Cuts at: 4872 4872
Size: 5209
BspHI T'CATG_A
Cuts at: 3098 4828 3098
Size: 1730 3479
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2403 2564 844
Size: 1559 161 3489
Fragments arranged by size:
3489 1559 161
BsrBI CCG'CTC
Cuts at: 1394 1823 3096 1394
Size: 429 1273 3507
Fragments arranged by size:
3507 1273 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2192 3704 3878 65
Size: 1183 944 1512 174 1396
Fragments arranged by size:
1512 1396 1183 944 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3853 4040 1700
Size: 110 2043 187 2869
Fragments arranged by size:
2869 2043 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5209
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5209
BssSI C'ACGA_G
Cuts at: 3269 4281 3269
Size: 1012 4197
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5209
BstBI TT'CG_AA
Cuts at: 4013 4013
Size: 5209
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5209
BstXI CCAn_nnnn'nTGG
Cuts at: 2062 2089 2062
Size: 27 5182
BstYI r'GATC_y
Cuts at: 1972 2460 2539 2906 3291 3308 4760 4846 4858
Size: 488 79 367 385 17 1452 86 12
Cuts at: 4858 5204 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 488 385 367 346 86 79
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2484 513
Size: 552 939 480 3238
Fragments arranged by size:
3238 939 552 480
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4470
Fragments arranged by size:
4470 683 56
BtsI GCAGTG_nn'
Cuts at: 2164 2725 3519 3539 2164
Size: 561 794 20 3834
Fragments arranged by size:
3834 794 561 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5209
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2404 2439 529
Size: 35 1840 35 3299
Fragments arranged by size:
3299 1840 35 35
DraI TTT'AAA
Cuts at: 2513 2513
Size: 5209
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4216 809
Size: 507 2900 1802
Fragments arranged by size:
2900 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2500 2656 3545
Size: 54 1425 50 275 18 670 156 889
Cuts at: 3545 5174 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 670 275 156 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2656 1537
Size: 275 844 4090
Fragments arranged by size:
4090 844 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3139 1353
Size: 1786 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2383 3796 4170 4316 387
Size: 1064 138 794 1413 374 146 1280
Fragments arranged by size:
1413 1280 1064 794 374 146 138
EcoICRI GAG'CTC
Cuts at: 2481 2481
Size: 5209
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2155 2190 2279 2320 2424 2454 3272
Size: 393 523 35 89 41 104 30 818
Cuts at: 3272 3869 4656 4924 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 818 787 597 523 393 268 104
89 41 35 30
EcoNI CCTnn'n_nnAGG
Cuts at: 2472 2472
Size: 5209
EcoRI G'AATT_C
Cuts at: 2518 2896 2518
Size: 378 4831
FspI TGC'GCA
Cuts at: 3715 3715
Size: 5209
HaeII r_GCGC'y
Cuts at: 1678 4356 1678
Size: 2678 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2384 2416 2452 2484 3850
Size: 32 135 32 812 32 36 32 1366
Cuts at: 3850 3882 3924 3956 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1366 812 135 42 36 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2553 2799 669
Size: 988 281 615 246 3079
Fragments arranged by size:
3079 988 615 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5209
HpaI GTT'AAC
Cuts at: 2799 2799
Size: 5209
KpnI G_GTAC'C
Cuts at: 2534 2534
Size: 5209
MfeI C'AATT_G
Cuts at: 2323 2808 2323
Size: 485 4724
MluI A'CGCG_T
Cuts at: 5085 5085
Size: 5209
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4323 4507 1305
Size: 3018 184 2007
Fragments arranged by size:
3018 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3387
Fragments arranged by size:
3387 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2129 2139 3167 3526 3685 518
Size: 648 963 10 1028 359 159 2042
Fragments arranged by size:
2042 1028 963 648 359 159 10
MspA1I CmG'CkG
Cuts at: 1606 2175 2625 3302 4450 4695 1606
Size: 569 450 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 569 450 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3718
Fragments arranged by size:
3718 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5209
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5209
NotI GC'GGCC_GC
Cuts at: 2656 2656
Size: 5209
NspI r_CATG'y
Cuts at: 1132 1229 2567 4112 1132
Size: 97 1338 1545 2229
Fragments arranged by size:
2229 1545 1338 97
PasI CC'CwG_GG
Cuts at: 1348 2275 1348
Size: 927 4282
PciI A'CATG_T
Cuts at: 4108 4108
Size: 5209
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5209
PfoI T'CCnGG_A
Cuts at: 2445 2470 2445
Size: 25 5184
PmeI GTTT'AAAC
Cuts at: 2513 2513
Size: 5209
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2273 2305 3278 3310 4791 4823 2273
Size: 32 973 32 1481 32 2659
Fragments arranged by size:
2659 1481 973 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5209
PsiI TTA'TAA
Cuts at: 2779 2779
Size: 5209
PstI C_TGCA'G
Cuts at: 830 2561 830
Size: 1731 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5209
PvuII CAG'CTG
Cuts at: 2175 2175
Size: 5209
SacI G_AGCT'C
Cuts at: 2483 2483
Size: 5209
SalI G'TCGA_C
Cuts at: 1936 2551 1936
Size: 615 4594
SbfI CC_TGCA'GG
Cuts at: 2561 2561
Size: 5209
ScaI AGT'ACT
Cuts at: 3457 5169 3457
Size: 1712 3497
SfcI C'TryA_G
Cuts at: 826 1045 2557 3692 4373 4564 826
Size: 219 1512 1135 681 191 1471
Fragments arranged by size:
1512 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5209
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5209
SmaI CCC'GGG
Cuts at: 1872 2184 2536 1872
Size: 312 352 4545
Fragments arranged by size:
4545 352 312
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3313 4214 4476 820
Size: 197 926 1370 901 262 1553
Fragments arranged by size:
1553 1370 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3000 493
Size: 2507 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5209
SphI G_CATG'C
Cuts at: 1132 2567 1132
Size: 1435 3774
SspI AAT'ATT
Cuts at: 5 52 3133 5
Size: 47 3081 2081
Fragments arranged by size:
3081 2081 47
StuI AGG'CCT
Cuts at: 1517 2061 2303 1517
Size: 544 242 4423
Fragments arranged by size:
4423 544 242
StyI C'CwwG_G
Cuts at: 513 1065 2004 2648 513
Size: 552 939 644 3074
Fragments arranged by size:
3074 939 644 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3236 3253 3406 3591 4036 325
Size: 1255 1656 17 153 185 445 1498
Fragments arranged by size:
1656 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2231 3455 5167
Size: 274 80 33 51 1443 254 1224 1712
Cuts at: 5167 96
Size: 138
Fragments arranged by size:
1712 1443 1224 274 254 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5169 5201 5169
Size: 32 5177
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5209
XbaI T'CTAG_A
Cuts at: 2545 2545
Size: 5209
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5209
XmaI C'CCGG_G
Cuts at: 1870 2182 2534 1870
Size: 312 352 4545
Fragments arranged by size:
4545 352 312
XmnI GAAnn'nnTTC
Cuts at: 2253 3338 2253
Size: 1085 4124
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3017 276
Size: 53 83 186 936 1483 2468
Fragments arranged by size:
2468 1483 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI