Restriction map for
pFN21AE0507
(Circular) (Six-base) MAPSORT of: pFN21AE0507.seq Check: 9297 from: 1 to: 5482
pFN21AE0507 5482 bp
With 182 enzymes: SgfI *
October 20, 2009 10:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2310 2310
Size: 5482
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3292 278
Size: 53 83 186 936 1756 2468
Fragments arranged by size:
2468 1756 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2825 1937
Size: 888 4594
Acc65I G'GTAC_C
Cuts at: 2803 2803
Size: 5482
AclI AA'CG_TT
Cuts at: 1563 3609 3982 1563
Size: 2046 373 3063
Fragments arranged by size:
3063 2046 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3545 4929 5197 1632
Size: 1913 1384 268 1917
Fragments arranged by size:
1917 1913 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5285
AflIII A'CryG_T
Cuts at: 1203 4381 5358 1203
Size: 3178 977 1327
Fragments arranged by size:
3178 1327 977
AgeI A'CCGG_T
Cuts at: 4313 4313
Size: 5482
AhdI GACnn_n'nnGTC
Cuts at: 4211 4211
Size: 5482
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5482
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2634 1184
Size: 1450 4032
AlwNI CAG_nnn'CTG
Cuts at: 2428 2472 3225 4797 2428
Size: 44 753 1572 3113
Fragments arranged by size:
3113 1572 753 44
ApaI G_GGCC'C
Cuts at: 2093 2191 2618 2743 2093
Size: 98 427 125 4832
Fragments arranged by size:
4832 427 125 98
ApaLI G'TGCA_C
Cuts at: 3539 4695 3539
Size: 1156 4326
ApoI r'AATT_y
Cuts at: 1493 2791 3017 3169 5278 1493
Size: 1298 226 152 2109 1697
Fragments arranged by size:
2109 1697 1298 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4036 160
Size: 872 3004 1606
Fragments arranged by size:
3004 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5482
AvaI C'yCGr_G
Cuts at: 1870 1943 2249 2355 2807 1870
Size: 73 306 106 452 4545
Fragments arranged by size:
4545 452 306 106 73
AvrII C'CTAG_G
Cuts at: 2632 2632
Size: 5482
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2378 2411 2378
Size: 33 5449
BamHI G'GATC_C
Cuts at: 2812 3179 2812
Size: 367 5115
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2803 4258 618
Size: 325 217 627 1016 1455 1842
Fragments arranged by size:
1842 1455 1016 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2093 2191 2618 2651 2743 2801 1391
Size: 702 98 427 33 92 58 4072
Fragments arranged by size:
4072 702 427 98 92 58 33
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4511
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2240 2274 3673 3707 1903
Size: 34 303 34 1399 34 3678
Fragments arranged by size:
3678 1399 303 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3374 4584 3374
Size: 1210 4272
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5482
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2333 2550 4093
Size: 107 122 71 631 605 661 217 1543
Cuts at: 4093 136
Size: 1525
Fragments arranged by size:
1543 1525 661 631 605 217 122 107
71
BglII A'GATC_T
Cuts at: 5477 5477
Size: 5482
BlpI GC'TnA_GC
Cuts at: 2899 2899
Size: 5482
Bme1580I G_kGCm'C
Cuts at: 1790 2093 2191 2250 2618 2743 3543 4699 1790
Size: 303 98 59 368 125 800 1156 2573
Fragments arranged by size:
2573 1156 800 368 303 125 98 59
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5482
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2106 3213 4171
Size: 416 53 83 399 24 689 1107 958
Cuts at: 4171 442
Size: 1753
Fragments arranged by size:
1753 1107 958 689 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5482
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2307 2531 4142 1239
Size: 1068 224 1611 2579
Fragments arranged by size:
2579 1611 1068 224
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2129 3607 4472 4770 2129
Size: 1478 865 298 2841
Fragments arranged by size:
2841 1478 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2657 4145 882
Size: 617 1158 1488 2219
Fragments arranged by size:
2219 1488 1158 617
BsaAI yAC'GTr
Cuts at: 493 1204 3273 493
Size: 711 2069 2702
Fragments arranged by size:
2702 2069 711
BsaBI GATnn'nnATC
Cuts at: 5476 5476
Size: 5482
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3289 3671 4306 275
Size: 53 83 186 936 1756 382 635 1451
Fragments arranged by size:
1756 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3915 4313 4587 4734 5145 3915
Size: 398 274 147 411 4252
Fragments arranged by size:
4252 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2390 2420 2513 2543 1178
Size: 30 165 30 987 30 93 30 4117
Fragments arranged by size:
4117 987 165 93 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2035 2267 2618 1186
Size: 849 232 351 4050
Fragments arranged by size:
4050 849 351 232
BseYI C'CCAG_C
Cuts at: 2053 4685 5181 2053
Size: 2632 496 2354
Fragments arranged by size:
2632 2354 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2203 1163
Size: 573 467 4442
Fragments arranged by size:
4442 573 467
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2932 3693 4721 1540
Size: 275 186 931 761 1028 2301
Fragments arranged by size:
2301 1028 931 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3543 3628 4699 3543
Size: 85 1071 4326
Fragments arranged by size:
4326 1071 85
BsmI GAATG_Cn'
Cuts at: 2993 3086 2993
Size: 93 5389
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2093 2191 2250 2618 2651 2743 2801
Size: 399 303 98 59 368 33 92 58
Cuts at: 2801 3543 3628 4699 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 368 303 98 92
85 59 58 33
BspEI T'CCGG_A
Cuts at: 5145 5145
Size: 5482
BspHI T'CATG_A
Cuts at: 3371 5101 3371
Size: 1730 3752
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2163 2310 2565 2837 844
Size: 1319 147 255 272 3489
Fragments arranged by size:
3489 1319 272 255 147
BsrBI CCG'CTC
Cuts at: 1394 1823 2445 3369 1394
Size: 429 622 924 3507
Fragments arranged by size:
3507 924 622 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3977 4151 65
Size: 1183 2729 174 1396
Fragments arranged by size:
2729 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2320 4126 4313 1700
Size: 110 510 1806 187 2869
Fragments arranged by size:
2869 1806 510 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5482
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5482
BssSI C'ACGA_G
Cuts at: 3542 4554 3542
Size: 1012 4470
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5482
BstBI TT'CG_AA
Cuts at: 4286 4286
Size: 5482
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5482
BstYI r'GATC_y
Cuts at: 1972 2812 3179 3564 3581 5033 5119 5131 5477
Size: 840 367 385 17 1452 86 12 346
Cuts at: 5477 1972
Size: 1977
Fragments arranged by size:
1977 1452 840 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4930
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4743
Fragments arranged by size:
4743 683 56
BtsI GCAGTG_nn'
Cuts at: 2521 2729 2998 3792 3812 2521
Size: 208 269 794 20 4191
Fragments arranged by size:
4191 794 269 208 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5482
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5447
DraI TTT'AAA
Cuts at: 2786 2786
Size: 5482
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4489 809
Size: 507 3173 1802
Fragments arranged by size:
3173 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2122 2232 2518
Size: 54 1425 50 275 18 292 110 286
Cuts at: 2518 2698 2929 3818 5447 8
Size: 180 231 889 1629 43
Fragments arranged by size:
1629 1425 889 292 286 275 231 180
110 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2929 1537
Size: 275 1117 4090
Fragments arranged by size:
4090 1117 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2729 3412 1353
Size: 1376 683 3423
Fragments arranged by size:
3423 1376 683
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4069 4443 4589 387
Size: 1064 138 2480 374 146 1280
Fragments arranged by size:
2480 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2307 2531 3545 4142 4929 5197 1239
Size: 393 675 224 1014 597 787 268 1524
Fragments arranged by size:
1524 1014 787 675 597 393 268 224
EcoNI CCTnn'n_nnAGG
Cuts at: 2531 2636 2531
Size: 105 5377
EcoO109I rG'GnC_Cy
Cuts at: 2089 2097 2188 2614 2615 2739 2089
Size: 8 91 426 1 124 4832
Fragments arranged by size:
4832 426 124 91 8 1
EcoRI G'AATT_C
Cuts at: 2791 3169 2791
Size: 378 5104
FspI TGC'GCA
Cuts at: 2078 3988 2078
Size: 1910 3572
HaeII r_GCGC'y
Cuts at: 1678 4629 1678
Size: 2951 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4123 4155 4197 4229 1373
Size: 32 135 32 2551 32 42 32 2626
Fragments arranged by size:
2626 2551 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2826 3072 669
Size: 988 281 888 246 3079
Fragments arranged by size:
3079 988 888 281 246
HindIII A'AGCT_T
Cuts at: 748 2362 748
Size: 1614 3868
HpaI GTT'AAC
Cuts at: 3072 3072
Size: 5482
KpnI G_GTAC'C
Cuts at: 2807 2807
Size: 5482
MfeI C'AATT_G
Cuts at: 3081 3081
Size: 5482
MluI A'CGCG_T
Cuts at: 5358 5358
Size: 5482
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2376 4596 4780 1305
Size: 1071 2220 184 2007
Fragments arranged by size:
2220 2007 1071 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2520 2700 10
Size: 54 1425 343 688 180 2792
Fragments arranged by size:
2792 1425 688 343 180 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3440 3799 3958 518
Size: 648 2274 359 159 2042
Fragments arranged by size:
2274 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2057 2472 2898 3575 4723 4968 1606
Size: 451 415 426 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 451 426 415 245
NaeI GCC'GGC
Cuts at: 2322 2322
Size: 5482
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4930
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5482
NgoMIV G'CCGG_C
Cuts at: 2320 2320
Size: 5482
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5482
NotI GC'GGCC_GC
Cuts at: 2929 2929
Size: 5482
NruI TCG'CGA
Cuts at: 2178 2178
Size: 5482
NspI r_CATG'y
Cuts at: 1132 1229 2377 2840 4385 1132
Size: 97 1148 463 1545 2229
Fragments arranged by size:
2229 1545 1148 463 97
PasI CC'CwG_GG
Cuts at: 1348 2093 2743 1348
Size: 745 650 4087
Fragments arranged by size:
4087 745 650
PciI A'CATG_T
Cuts at: 4381 4381
Size: 5482
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2428 1108
Size: 1320 4162
PfoI T'CCnGG_A
Cuts at: 2450 2450
Size: 5482
PmeI GTTT'AAAC
Cuts at: 2786 2786
Size: 5482
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3551 3583 5064 5096 3551
Size: 32 1481 32 3937
Fragments arranged by size:
3937 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2097 2097
Size: 5482
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5482
PsiI TTA'TAA
Cuts at: 3052 3052
Size: 5482
PspOMI G'GGCC_C
Cuts at: 2089 2187 2614 2739 2089
Size: 98 427 125 4832
Fragments arranged by size:
4832 427 125 98
PstI C_TGCA'G
Cuts at: 830 2314 2584 2613 2834 830
Size: 1484 270 29 221 3478
Fragments arranged by size:
3478 1484 270 221 29
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5482
SalI G'TCGA_C
Cuts at: 1936 2824 1936
Size: 888 4594
SanDI GG'GwC_CC
Cuts at: 2097 2097
Size: 5482
SbfI CC_TGCA'GG
Cuts at: 2834 2834
Size: 5482
ScaI AGT'ACT
Cuts at: 3730 5442 3730
Size: 1712 3770
SfcI C'TryA_G
Cuts at: 826 1045 2310 2580 2609 2830 3965 4646 4837
Size: 219 1265 270 29 221 1135 681 191
Cuts at: 4837 826
Size: 1471
Fragments arranged by size:
1471 1265 1135 681 270 221 219 191
29
SfiI GGCCn_nnn'nGGCC
Cuts at: 2333 2333
Size: 5482
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5482
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5482
SmaI CCC'GGG
Cuts at: 1872 2357 2809 1872
Size: 485 452 4545
Fragments arranged by size:
4545 485 452
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2144 3586 4487 4749 820
Size: 197 926 201 1442 901 262 1553
Fragments arranged by size:
1553 1442 926 901 262 201 197
SnaBI TAC'GTA
Cuts at: 493 3273 493
Size: 2780 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5482
SphI G_CATG'C
Cuts at: 1132 2840 1132
Size: 1708 3774
SspI AAT'ATT
Cuts at: 5 52 3406 5
Size: 47 3354 2081
Fragments arranged by size:
3354 2081 47
StuI AGG'CCT
Cuts at: 1517 2336 1517
Size: 819 4663
StyI C'CwwG_G
Cuts at: 513 1065 2532 2632 2921 513
Size: 552 1467 100 289 3074
Fragments arranged by size:
3074 1467 552 289 100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2329 3509 3526 3679 3864 4309 325
Size: 1255 749 1180 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1180 749 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3728 5440 96
Size: 274 80 33 51 1443 1751 1712 138
Fragments arranged by size:
1751 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2513 2545 5442 5474 2513
Size: 32 2897 32 2521
Fragments arranged by size:
2897 2521 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5482
XbaI T'CTAG_A
Cuts at: 2818 2818
Size: 5482
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5482
XmaI C'CCGG_G
Cuts at: 1870 2355 2807 1870
Size: 485 452 4545
Fragments arranged by size:
4545 485 452
XmnI GAAnn'nnTTC
Cuts at: 3611 3611
Size: 5482
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3290 276
Size: 53 83 186 936 1756 2468
Fragments arranged by size:
2468 1756 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NruI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI
SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BbeI BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NarI NsiI PacI PmlI
PspXI PsrI PvuII RsrII SacI SacII SapI SexAI
SfoI SrfI SwaI XcmI