Restriction map for
pFN21AE0502
(Circular) (Six-base) MAPSORT of: pFN21AE0502.seq Check: 177 from: 1 to: 5338
pFN21AE0502 5338 bp
With 182 enzymes: SgfI *
October 20, 2009 10:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3148 278
Size: 53 83 186 936 1612 2468
Fragments arranged by size:
2468 1612 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2681 1937
Size: 744 4594
Acc65I G'GTAC_C
Cuts at: 2659 2659
Size: 5338
AclI AA'CG_TT
Cuts at: 1563 3465 3838 1563
Size: 1902 373 3063
Fragments arranged by size:
3063 1902 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3401 4785 5053 1632
Size: 1769 1384 268 1917
Fragments arranged by size:
1917 1769 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5141
AflIII A'CryG_T
Cuts at: 1203 2517 4237 5214 1203
Size: 1314 1720 977 1327
Fragments arranged by size:
1720 1327 1314 977
AgeI A'CCGG_T
Cuts at: 4169 4169
Size: 5338
AhdI GACnn_n'nnGTC
Cuts at: 4067 4067
Size: 5338
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5338
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5338
AlwNI CAG_nnn'CTG
Cuts at: 3081 4653 3081
Size: 1572 3766
ApaLI G'TGCA_C
Cuts at: 3395 4551 3395
Size: 1156 4182
ApoI r'AATT_y
Cuts at: 1493 2647 2873 3025 5134 1493
Size: 1154 226 152 2109 1697
Fragments arranged by size:
2109 1697 1154 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3892 160
Size: 872 2860 1606
Fragments arranged by size:
2860 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5338
AvaI C'yCGr_G
Cuts at: 1870 1943 2663 1870
Size: 73 720 4545
Fragments arranged by size:
4545 720 73
BamHI G'GATC_C
Cuts at: 2668 3035 2668
Size: 367 4971
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2508 2659 4114 618
Size: 325 217 627 721 151 1455 1842
Fragments arranged by size:
1842 1455 721 627 325 217 151
BanII G_rGCy'C
Cuts at: 1391 2127 2177 2428 2657 1391
Size: 736 50 251 229 4072
Fragments arranged by size:
4072 736 251 229 50
BbeI G_GCGC'C
Cuts at: 2512 2512
Size: 5338
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4367
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2018 2052 3529 3563 1903
Size: 34 81 34 1477 34 3678
Fragments arranged by size:
3678 1477 81 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3230 4440 3230
Size: 1210 4128
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5338
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3949 136
Size: 107 122 71 631 605 2277 1525
Fragments arranged by size:
2277 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5333 5333
Size: 5338
BlpI GC'TnA_GC
Cuts at: 2755 2755
Size: 5338
Bme1580I G_kGCm'C
Cuts at: 1790 2141 2415 3399 4555 1790
Size: 351 274 984 1156 2573
Fragments arranged by size:
2573 1156 984 351 274
BmgBI CAC'GTC
Cuts at: 1318 2410 2475 1318
Size: 1092 65 4181
Fragments arranged by size:
4181 1092 65
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3069 4027 442
Size: 416 53 83 399 24 1652 958 1753
Fragments arranged by size:
1753 1652 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5338
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2183 2267 3998 1239
Size: 944 84 1731 2579
Fragments arranged by size:
2579 1731 944 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3463 4328 4626 3463
Size: 865 298 4175
Fragments arranged by size:
4175 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2052 4001 882
Size: 617 553 1949 2219
Fragments arranged by size:
2219 1949 617 553
BsaAI yAC'GTr
Cuts at: 493 1204 2518 3129 493
Size: 711 1314 611 2702
Fragments arranged by size:
2702 1314 711 611
BsaBI GATnn'nnATC
Cuts at: 5332 5332
Size: 5338
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2509 2559 3145 3527
Size: 53 83 186 936 976 50 586 382
Cuts at: 3527 4162 275
Size: 635 1451
Fragments arranged by size:
1451 976 936 635 586 382 186 83
53 50
BsaWI w'CCGG_w
Cuts at: 3771 4169 4443 4590 5001 3771
Size: 398 274 147 411 4108
Fragments arranged by size:
4108 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5113
Fragments arranged by size:
5113 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5338
BseYI C'CCAG_C
Cuts at: 2141 2268 2368 2414 2630 4541 5037 2141
Size: 127 100 46 216 1911 496 2442
Fragments arranged by size:
2442 1911 496 216 127 100 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4765
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2133 2788 3549 4577 1540
Size: 275 186 132 655 761 1028 2301
Fragments arranged by size:
2301 1028 761 655 275 186 132
BsiHKAI G_wGCw'C
Cuts at: 2026 2127 3399 3484 4555 2026
Size: 101 1272 85 1071 2809
Fragments arranged by size:
2809 1272 1071 101 85
BsmI GAATG_Cn'
Cuts at: 2849 2942 2849
Size: 93 5245
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2026 2127 2141 2177 2415 2428 2657
Size: 399 236 101 14 36 238 13 229
Cuts at: 2657 3399 3484 4555 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 238 236 229 101
85 36 14 13
BspEI T'CCGG_A
Cuts at: 5001 5001
Size: 5338
BspHI T'CATG_A
Cuts at: 3227 4957 3227
Size: 1730 3608
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2693 844
Size: 1849 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2527 3225 1394
Size: 429 704 698 3507
Fragments arranged by size:
3507 704 698 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3833 4007 65
Size: 1183 2585 174 1396
Fragments arranged by size:
2585 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3982 4169 1700
Size: 110 2172 187 2869
Fragments arranged by size:
2869 2172 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5338
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5338
BssSI C'ACGA_G
Cuts at: 3398 4410 3398
Size: 1012 4326
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2118 2584 794
Size: 1324 466 3548
Fragments arranged by size:
3548 1324 466
BstBI TT'CG_AA
Cuts at: 4142 4142
Size: 5338
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5338
BstXI CCAn_nnnn'nTGG
Cuts at: 2185 2185
Size: 5338
BstYI r'GATC_y
Cuts at: 1972 2668 3035 3420 3437 4889 4975 4987 5333
Size: 696 367 385 17 1452 86 12 346
Cuts at: 5333 1972
Size: 1977
Fragments arranged by size:
1977 1452 696 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2053 2198 2227 2503 513
Size: 552 939 49 145 29 276 3348
Fragments arranged by size:
3348 939 552 276 145 49 29
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4599
Fragments arranged by size:
4599 683 56
BtsI GCAGTG_nn'
Cuts at: 2854 3648 3668 2854
Size: 794 20 4524
Fragments arranged by size:
4524 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5338
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5303
DraI TTT'AAA
Cuts at: 2642 2642
Size: 5338
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4345 809
Size: 507 3029 1802
Fragments arranged by size:
3029 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2130 2380 2785
Size: 54 1425 50 275 18 300 250 405
Cuts at: 2785 3674 5303 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 405 300 275 250 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2130 2785 1537
Size: 275 318 655 4090
Fragments arranged by size:
4090 655 318 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3268 1353
Size: 1915 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 3925 4299 4445 387
Size: 1064 138 435 1901 374 146 1280
Fragments arranged by size:
1901 1280 1064 435 374 146 138
EcoICRI GAG'CTC
Cuts at: 2125 2125
Size: 5338
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2183 2267 3401 3998 4785 5053 1239
Size: 393 551 84 1134 597 787 268 1524
Fragments arranged by size:
1524 1134 787 597 551 393 268 84
EcoO109I rG'GnC_Cy
Cuts at: 2220 2220
Size: 5338
EcoRI G'AATT_C
Cuts at: 2647 3025 2647
Size: 378 4960
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2101 2101
Size: 5338
FspI TGC'GCA
Cuts at: 3844 3844
Size: 5338
HaeII r_GCGC'y
Cuts at: 1678 2512 4485 1678
Size: 834 1973 2531
Fragments arranged by size:
2531 1973 834
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2264 2296 3979 4011 4053
Size: 32 135 32 692 32 1683 32 42
Cuts at: 4053 4085 1373
Size: 32 2626
Fragments arranged by size:
2626 1683 692 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2682 2928 669
Size: 988 281 744 246 3079
Fragments arranged by size:
3079 988 744 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5338
HpaI GTT'AAC
Cuts at: 2928 2928
Size: 5338
KasI G'GCGC_C
Cuts at: 2508 2508
Size: 5338
KpnI G_GTAC'C
Cuts at: 2663 2663
Size: 5338
MfeI C'AATT_G
Cuts at: 2937 2937
Size: 5338
MluI A'CGCG_T
Cuts at: 5214 5214
Size: 5338
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2307 4452 4636 1305
Size: 1002 2145 184 2007
Fragments arranged by size:
2145 2007 1002 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3516
Fragments arranged by size:
3516 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2183 2547 3296 3655 3814 518
Size: 648 1017 364 749 359 159 2042
Fragments arranged by size:
2042 1017 749 648 364 359 159
MspA1I CmG'CkG
Cuts at: 1606 2042 2229 2575 2754 3431 4579 4824 1606
Size: 436 187 346 179 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 436 346 245 187 179
NarI GG'CG_CC
Cuts at: 2509 2509
Size: 5338
NcoI C'CATG_G
Cuts at: 513 1065 2004 2053 513
Size: 552 939 49 3798
Fragments arranged by size:
3798 939 552 49
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5338
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5338
NotI GC'GGCC_GC
Cuts at: 2785 2785
Size: 5338
NspI r_CATG'y
Cuts at: 1132 1229 2696 4241 1132
Size: 97 1467 1545 2229
Fragments arranged by size:
2229 1545 1467 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5338
PciI A'CATG_T
Cuts at: 4237 4237
Size: 5338
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2421 1108
Size: 1313 4025
PfoI T'CCnGG_A
Cuts at: 2436 2436
Size: 5338
PmeI GTTT'AAAC
Cuts at: 2642 2642
Size: 5338
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3407 3439 4920 4952 3407
Size: 32 1481 32 3793
Fragments arranged by size:
3793 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5338
PsiI TTA'TAA
Cuts at: 2908 2908
Size: 5338
PstI C_TGCA'G
Cuts at: 830 2041 2580 2690 830
Size: 1211 539 110 3478
Fragments arranged by size:
3478 1211 539 110
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5338
PvuII CAG'CTG
Cuts at: 2042 2575 2042
Size: 533 4805
SacI G_AGCT'C
Cuts at: 2127 2127
Size: 5338
SacII CC_GC'GG
Cuts at: 2230 2230
Size: 5338
SalI G'TCGA_C
Cuts at: 1936 2680 1936
Size: 744 4594
SbfI CC_TGCA'GG
Cuts at: 2690 2690
Size: 5338
ScaI AGT'ACT
Cuts at: 3586 5298 3586
Size: 1712 3626
SfcI C'TryA_G
Cuts at: 826 1045 2037 2576 2686 3821 4502 4693 826
Size: 219 992 539 110 1135 681 191 1471
Fragments arranged by size:
1471 1135 992 681 539 219 191 110
SfoI GGC'GCC
Cuts at: 2510 2510
Size: 5338
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5338
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5338
SmaI CCC'GGG
Cuts at: 1872 2665 1872
Size: 793 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3442 4343 4605 820
Size: 197 926 1499 901 262 1553
Fragments arranged by size:
1553 1499 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3129 493
Size: 2636 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5338
SphI G_CATG'C
Cuts at: 1132 2696 1132
Size: 1564 3774
SspI AAT'ATT
Cuts at: 5 52 2329 3262 5
Size: 47 2277 933 2081
Fragments arranged by size:
2277 2081 933 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5338
StyI C'CwwG_G
Cuts at: 513 1065 2004 2053 2062 2107 2777 513
Size: 552 939 49 9 45 670 3074
Fragments arranged by size:
3074 939 670 552 49 45 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2165 3365 3382 3535 3720 4165 325
Size: 1255 585 1200 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1200 585 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3584 5296 96
Size: 274 80 33 51 1443 1607 1712 138
Fragments arranged by size:
1712 1607 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5298 5330 5298
Size: 32 5306
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5338
XbaI T'CTAG_A
Cuts at: 2674 2674
Size: 5338
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2060 2060
Size: 5338
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5338
XmaI C'CCGG_G
Cuts at: 1870 2663 1870
Size: 793 4545
XmnI GAAnn'nnTTC
Cuts at: 3467 3467
Size: 5338
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3146 276
Size: 53 83 186 936 1612 2468
Fragments arranged by size:
2468 1612 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SacI SacII SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI
PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SanDI
SapI SexAI SfiI SrfI SwaI