Restriction map for
pFN21AE0499
(Circular) (Six-base) MAPSORT of: pFN21AE0499.seq Check: 8977 from: 1 to: 6742
pFN21AE0499 6742 bp
With 182 enzymes: SgfI *
February 26, 2010 09:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4552 278
Size: 53 83 186 936 3016 2468
Fragments arranged by size:
3016 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4085 1937
Size: 2148 4594
Acc65I G'GTAC_C
Cuts at: 2724 4063 2724
Size: 1339 5403
AclI AA'CG_TT
Cuts at: 1563 4869 5242 1563
Size: 3306 373 3063
Fragments arranged by size:
3306 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3891 4805 6189 6457 1632
Size: 2259 914 1384 268 1917
Fragments arranged by size:
2259 1917 1384 914 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6545
AflIII A'CryG_T
Cuts at: 1203 5641 6618 1203
Size: 4438 977 1327
Fragments arranged by size:
4438 1327 977
AgeI A'CCGG_T
Cuts at: 5573 5573
Size: 6742
AhdI GACnn_n'nnGTC
Cuts at: 5471 5471
Size: 6742
AleI CACnn'nnGTG
Cuts at: 1166 3455 1166
Size: 2289 4453
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6742
AlwNI CAG_nnn'CTG
Cuts at: 4485 6057 4485
Size: 1572 5170
ApaLI G'TGCA_C
Cuts at: 2274 4799 5955 2274
Size: 2525 1156 3061
Fragments arranged by size:
3061 2525 1156
ApoI r'AATT_y
Cuts at: 1493 2366 2934 3027 3278 4051 4277 4429 6538
Size: 873 568 93 251 773 226 152 2109
Cuts at: 6538 1493
Size: 1697
Fragments arranged by size:
2109 1697 873 773 568 251 226 152
93
AseI AT'TA_AT
Cuts at: 160 1032 3366 5296 160
Size: 872 2334 1930 1606
Fragments arranged by size:
2334 1930 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6742
AvaI C'yCGr_G
Cuts at: 1870 1943 2952 4067 1870
Size: 73 1009 1115 4545
Fragments arranged by size:
4545 1115 1009 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3475 3508 3475
Size: 33 6709
BamHI G'GATC_C
Cuts at: 4072 4439 4072
Size: 367 6375
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2724 4063 5518 618
Size: 325 217 627 937 1339 1455 1842
Fragments arranged by size:
1842 1455 1339 937 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2479 4061 1391
Size: 1088 1582 4072
Fragments arranged by size:
4072 1582 1088
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2295 928
Size: 971 396 5375
Fragments arranged by size:
5375 971 396
BbvCI CC'TCA_GC
Cuts at: 2176 2176
Size: 6742
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3881 3915 4933 4967 1903
Size: 34 1944 34 1018 34 3678
Fragments arranged by size:
3678 1944 1018 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4634 5844 4634
Size: 1210 5532
BclI T'GATC_A
Cuts at: 1548 2523 1548
Size: 975 5767
BfrBI ATG'CAT
Cuts at: 2942 2942
Size: 6742
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5353 136
Size: 107 122 71 631 605 3681 1525
Fragments arranged by size:
3681 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2647 6737 2647
Size: 4090 2652
BlpI GC'TnA_GC
Cuts at: 4159 4159
Size: 6742
Bme1580I G_kGCm'C
Cuts at: 1790 2278 4039 4803 5959 1790
Size: 488 1761 764 1156 2573
Fragments arranged by size:
2573 1761 1156 764 488
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6742
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2139 2277 4473
Size: 416 53 83 399 24 722 138 2196
Cuts at: 4473 5431 442
Size: 958 1753
Fragments arranged by size:
2196 1753 958 722 416 399 138 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6742
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3699 3699
Size: 6742
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2111 2151 2328 3501 5402 1239
Size: 872 40 177 1173 1901 2579
Fragments arranged by size:
2579 1901 1173 872 177 40
Bpu10I CC'TnA_GC
Cuts at: 2176 2176
Size: 6742
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3371 3944 4867 5732 6030 3371
Size: 573 923 865 298 4083
Fragments arranged by size:
4083 923 865 573 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4021 5405 882
Size: 617 2522 1384 2219
Fragments arranged by size:
2522 2219 1384 617
BsaAI yAC'GTr
Cuts at: 493 1204 2273 2384 4533 493
Size: 711 1069 111 2149 2702
Fragments arranged by size:
2702 2149 1069 711 111
BsaBI GATnn'nnATC
Cuts at: 6736 6736
Size: 6742
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4549 4931 5566 275
Size: 53 83 186 936 3016 382 635 1451
Fragments arranged by size:
3016 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3191 5175 5573 5847 5994 6405 3191
Size: 1984 398 274 147 411 3528
Fragments arranged by size:
3528 1984 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3499 3529 1178
Size: 30 165 30 2096 30 4391
Fragments arranged by size:
4391 2096 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3327 4007 1186
Size: 2141 680 3921
Fragments arranged by size:
3921 2141 680
BseYI C'CCAG_C
Cuts at: 5945 6441 5945
Size: 496 6246
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2295 3999 1163
Size: 573 559 1704 3906
Fragments arranged by size:
3906 1704 573 559
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4192 4953 5981 1540
Size: 275 186 2191 761 1028 2301
Fragments arranged by size:
2301 2191 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2278 4803 4888 5959 2278
Size: 2525 85 1071 3061
Fragments arranged by size:
3061 2525 1071 85
BsmI GAATG_Cn'
Cuts at: 4253 4346 4253
Size: 93 6649
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2278 2479 4039 4061 4803 4888 5959
Size: 399 488 201 1560 22 742 85 1071
Cuts at: 5959 1391
Size: 2174
Fragments arranged by size:
2174 1560 1071 742 488 399 201 85
22
BspEI T'CCGG_A
Cuts at: 6405 6405
Size: 6742
BspHI T'CATG_A
Cuts at: 4631 6361 4631
Size: 1730 5012
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3091 3503 4097 844
Size: 2247 412 594 3489
Fragments arranged by size:
3489 2247 594 412
BsrBI CCG'CTC
Cuts at: 1394 1823 4629 1394
Size: 429 2806 3507
Fragments arranged by size:
3507 2806 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5237 5411 65
Size: 1183 3989 174 1396
Fragments arranged by size:
3989 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5386 5573 1700
Size: 110 3576 187 2869
Fragments arranged by size:
3576 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6742
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6742
BssSI C'ACGA_G
Cuts at: 4802 5814 4802
Size: 1012 5730
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3180 794
Size: 2386 4356
BstBI TT'CG_AA
Cuts at: 5546 5546
Size: 6742
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6742
BstYI r'GATC_y
Cuts at: 1972 2572 2647 3589 4072 4439 4824 4841 6293
Size: 600 75 942 483 367 385 17 1452
Cuts at: 6293 6379 6391 6737 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 942 600 483 385 367 346
86 75 17 12
Bsu36I CC'TnA_GG
Cuts at: 3406 3406
Size: 6742
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5251
Fragments arranged by size:
5251 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6003
Fragments arranged by size:
6003 683 56
BtsI GCAGTG_nn'
Cuts at: 3240 3819 4258 5052 5072 3240
Size: 579 439 794 20 4910
Fragments arranged by size:
4910 794 579 439 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6742
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6707
DraI TTT'AAA
Cuts at: 2019 4046 2019
Size: 2027 4715
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5749 809
Size: 507 4433 1802
Fragments arranged by size:
4433 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4189 5078 6707
Size: 54 1425 50 275 18 2359 889 1629
Cuts at: 6707 8
Size: 43
Fragments arranged by size:
2359 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4189 1537
Size: 275 2377 4090
Fragments arranged by size:
4090 2377 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4019 4672 1353
Size: 2666 653 3423
Fragments arranged by size:
3423 2666 653
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5329 5703 5849 387
Size: 1064 138 3740 374 146 1280
Fragments arranged by size:
3740 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2111 2151 2328 3501 3891 4805 5402
Size: 393 479 40 177 1173 390 914 597
Cuts at: 5402 6189 6457 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1173 914 787 597 479 393 390
268 177 40
EcoO109I rG'GnC_Cy
Cuts at: 3853 3853
Size: 6742
EcoRI G'AATT_C
Cuts at: 4051 4429 4051
Size: 378 6364
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3486 3874 3938 3486
Size: 388 64 6290
Fragments arranged by size:
6290 388 64
FspI TGC'GCA
Cuts at: 5248 5248
Size: 6742
HaeII r_GCGC'y
Cuts at: 1678 5889 1678
Size: 4211 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3574 3606 3679 3711 5383
Size: 32 135 32 2002 32 73 32 1672
Cuts at: 5383 5415 5457 5489 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2002 1672 135 73 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4086 4332 669
Size: 988 281 2148 246 3079
Fragments arranged by size:
3079 2148 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6742
HpaI GTT'AAC
Cuts at: 4332 4332
Size: 6742
KpnI G_GTAC'C
Cuts at: 2728 4067 2728
Size: 1339 5403
MfeI C'AATT_G
Cuts at: 4341 4341
Size: 6742
MluI A'CGCG_T
Cuts at: 6618 6618
Size: 6742
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3617 5856 6040 1305
Size: 2312 2239 184 2007
Fragments arranged by size:
2312 2239 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4920
Fragments arranged by size:
4920 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3455 4700 5059 5218 518
Size: 648 2289 1245 359 159 2042
Fragments arranged by size:
2289 2042 1245 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4158 4835 5983 6228 1606
Size: 2552 677 1148 245 2120
Fragments arranged by size:
2552 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5251
Fragments arranged by size:
5251 939 552
NdeI CA'TA_TG
Cuts at: 387 3898 387
Size: 3511 3231
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6742
NotI GC'GGCC_GC
Cuts at: 4189 4189
Size: 6742
NsiI A_TGCA'T
Cuts at: 2944 2944
Size: 6742
NspI r_CATG'y
Cuts at: 1132 1229 2850 4100 5645 1132
Size: 97 1621 1250 1545 2229
Fragments arranged by size:
2229 1621 1545 1250 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6742
PciI A'CATG_T
Cuts at: 5641 5641
Size: 6742
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6742
PfoI T'CCnGG_A
Cuts at: 3517 3819 3517
Size: 302 6440
PmeI GTTT'AAAC
Cuts at: 4046 4046
Size: 6742
PmlI CAC'GTG
Cuts at: 2273 2273
Size: 6742
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4811 4843 6324 6356 4811
Size: 32 1481 32 5197
Fragments arranged by size:
5197 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6742
PsiI TTA'TAA
Cuts at: 4312 4312
Size: 6742
PstI C_TGCA'G
Cuts at: 830 4094 830
Size: 3264 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6742
SalI G'TCGA_C
Cuts at: 1936 4084 1936
Size: 2148 4594
SbfI CC_TGCA'GG
Cuts at: 4094 4094
Size: 6742
ScaI AGT'ACT
Cuts at: 4990 6702 4990
Size: 1712 5030
SfcI C'TryA_G
Cuts at: 826 1045 2214 3585 4090 5225 5906 6097 826
Size: 219 1169 1371 505 1135 681 191 1471
Fragments arranged by size:
1471 1371 1169 1135 681 505 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6742
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6742
SmaI CCC'GGG
Cuts at: 1872 4069 1872
Size: 2197 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2952 3386 3923 4846 5747 6009
Size: 197 926 1009 434 537 923 901 262
Cuts at: 6009 820
Size: 1553
Fragments arranged by size:
1553 1009 926 923 901 537 434 262
197
SnaBI TAC'GTA
Cuts at: 493 4533 493
Size: 4040 2702
SpeI A'CTAG_T
Cuts at: 152 2812 152
Size: 2660 4082
SphI G_CATG'C
Cuts at: 1132 4100 1132
Size: 2968 3774
SspI AAT'ATT
Cuts at: 5 52 4666 5
Size: 47 4614 2081
Fragments arranged by size:
4614 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6742
StyI C'CwwG_G
Cuts at: 513 1065 2004 4181 513
Size: 552 939 2177 3074
Fragments arranged by size:
3074 2177 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2882 4769 4786 4939 5124 5569 325
Size: 1255 1302 1887 17 153 185 445 1498
Fragments arranged by size:
1887 1498 1302 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3151 4988 6700
Size: 274 80 33 51 1443 1174 1837 1712
Cuts at: 6700 96
Size: 138
Fragments arranged by size:
1837 1712 1443 1174 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3499 3531 6702 6734 3499
Size: 32 3171 32 3507
Fragments arranged by size:
3507 3171 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6742
XbaI T'CTAG_A
Cuts at: 3082 4078 3082
Size: 996 5746
XhoI C'TCGA_G
Cuts at: 1943 2952 1943
Size: 1009 5733
XmaI C'CCGG_G
Cuts at: 1870 4067 1870
Size: 2197 4545
XmnI GAAnn'nnTTC
Cuts at: 3828 3918 3971 4871 3828
Size: 90 53 900 5699
Fragments arranged by size:
5699 900 90 53
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4550 276
Size: 53 83 186 936 3016 2468
Fragments arranged by size:
3016 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BsiWI
BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI