Restriction map for
pFN21AE0493
(Circular) (Six-base) MAPSORT of: pFN21AE0493.seq Check: 9626 from: 1 to: 5890
pFN21AE0493 5890 bp
With 182 enzymes: SgfI *
October 20, 2009 10:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2283 2283
Size: 5890
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3700 278
Size: 53 83 186 936 2164 2468
Fragments arranged by size:
2468 2164 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2696 3233 1937
Size: 759 537 4594
Fragments arranged by size:
4594 759 537
Acc65I G'GTAC_C
Cuts at: 2586 3211 2586
Size: 625 5265
AclI AA'CG_TT
Cuts at: 1563 4017 4390 1563
Size: 2454 373 3063
Fragments arranged by size:
3063 2454 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2678 3953 5337 5605 1632
Size: 1046 1275 1384 268 1917
Fragments arranged by size:
1917 1384 1275 1046 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5693
AflIII A'CryG_T
Cuts at: 1203 4789 5766 1203
Size: 3586 977 1327
Fragments arranged by size:
3586 1327 977
AgeI A'CCGG_T
Cuts at: 4721 4721
Size: 5890
AhdI GACnn_n'nnGTC
Cuts at: 4619 4619
Size: 5890
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5890
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2555 1184
Size: 1371 4519
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2430 2462 2430
Size: 32 5858
AlwNI CAG_nnn'CTG
Cuts at: 2608 3633 5205 2608
Size: 1025 1572 3293
Fragments arranged by size:
3293 1572 1025
ApaI G_GGCC'C
Cuts at: 2403 2403
Size: 5890
ApaLI G'TGCA_C
Cuts at: 3947 5103 3947
Size: 1156 4734
ApoI r'AATT_y
Cuts at: 1493 3104 3199 3425 3577 5686 1493
Size: 1611 95 226 152 2109 1697
Fragments arranged by size:
2109 1697 1611 226 152 95
AscI GG'CGCG_CC
Cuts at: 2113 2113
Size: 5890
AseI AT'TA_AT
Cuts at: 160 1032 4444 160
Size: 872 3412 1606
Fragments arranged by size:
3412 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5890
AvaI C'yCGr_G
Cuts at: 1870 1943 2201 2298 3215 1870
Size: 73 258 97 917 4545
Fragments arranged by size:
4545 917 258 97 73
BamHI G'GATC_C
Cuts at: 3220 3587 3220
Size: 367 5523
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2160 2187 2586 3211 4666
Size: 325 217 627 373 27 399 625 1455
Cuts at: 4666 618
Size: 1842
Fragments arranged by size:
1842 1455 627 625 399 373 325 217
27
BanII G_rGCy'C
Cuts at: 1391 2298 2403 2559 3209 1391
Size: 907 105 156 650 4072
Fragments arranged by size:
4072 907 650 156 105
BbeI G_GCGC'C
Cuts at: 2191 2191
Size: 5890
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2410 2707 928
Size: 971 511 297 4111
Fragments arranged by size:
4111 971 511 297
BbvCI CC'TCA_GC
Cuts at: 2210 2237 2210
Size: 27 5863
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2924 2958 4081 4115 1903
Size: 34 987 34 1123 34 3678
Fragments arranged by size:
3678 1123 987 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3782 4992 3782
Size: 1210 4680
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5890
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2563 4501
Size: 107 122 71 631 605 337 554 1938
Cuts at: 4501 136
Size: 1525
Fragments arranged by size:
1938 1525 631 605 554 337 122 107
71
BglII A'GATC_T
Cuts at: 5885 5885
Size: 5890
BlpI GC'TnA_GC
Cuts at: 3307 3307
Size: 5890
Bme1580I G_kGCm'C
Cuts at: 1790 2090 2403 3951 5107 1790
Size: 300 313 1548 1156 2573
Fragments arranged by size:
2573 1548 1156 313 300
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5890
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3121 3621 4579
Size: 416 53 83 399 24 1704 500 958
Cuts at: 4579 442
Size: 1753
Fragments arranged by size:
1753 1704 958 500 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5890
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2998 2998
Size: 5890
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2663 3065 4550 1239
Size: 1424 402 1485 2579
Fragments arranged by size:
2579 1485 1424 402
Bpu10I CC'TnA_GC
Cuts at: 2210 2237 2210
Size: 27 5863
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2454 2904 4015 4880 5178 2454
Size: 450 1111 865 298 3166
Fragments arranged by size:
3166 1111 865 450 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2969 4553 882
Size: 617 1470 1584 2219
Fragments arranged by size:
2219 1584 1470 617
BsaAI yAC'GTr
Cuts at: 493 1204 3681 493
Size: 711 2477 2702
Fragments arranged by size:
2702 2477 711
BsaBI GATnn'nnATC
Cuts at: 5884 5884
Size: 5890
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2136 2188 3697 4079
Size: 53 83 186 936 603 52 1509 382
Cuts at: 4079 4714 275
Size: 635 1451
Fragments arranged by size:
1509 1451 936 635 603 382 186 83
53 52
BsaWI w'CCGG_w
Cuts at: 4323 4721 4995 5142 5553 4323
Size: 398 274 147 411 4660
Fragments arranged by size:
4660 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5665
Fragments arranged by size:
5665 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2219 2306 3005 3172 1186
Size: 1033 87 699 167 3904
Fragments arranged by size:
3904 1033 699 167 87
BseYI C'CCAG_C
Cuts at: 2279 2395 2403 5093 5589 2279
Size: 116 8 2690 496 2580
Fragments arranged by size:
2690 2580 496 116 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2216 1163
Size: 573 480 4837
Fragments arranged by size:
4837 573 480
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2013 2393 3340 4101 5129 1540
Size: 275 186 12 380 947 761 1028 2301
Fragments arranged by size:
2301 1028 947 761 380 275 186 12
BsiHKAI G_wGCw'C
Cuts at: 3951 4036 5107 3951
Size: 85 1071 4734
Fragments arranged by size:
4734 1071 85
BsmI GAATG_Cn'
Cuts at: 3401 3494 3401
Size: 93 5797
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2090 2298 2403 2559 3209 3951 4036
Size: 399 300 208 105 156 650 742 85
Cuts at: 4036 5107 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 650 399 300 208 156
105 85
BspEI T'CCGG_A
Cuts at: 5553 5553
Size: 5890
BspHI T'CATG_A
Cuts at: 3779 5509 3779
Size: 1730 4160
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2253 2283 2616 2646 3245 844
Size: 1409 30 333 30 599 3489
Fragments arranged by size:
3489 1409 599 333 30 30
BsrBI CCG'CTC
Cuts at: 1394 1823 2229 2389 2584 3777 1394
Size: 429 406 160 195 1193 3507
Fragments arranged by size:
3507 1193 429 406 195 160
BsrDI GCAATG_nn'
Cuts at: 65 1248 2502 4385 4559 65
Size: 1183 1254 1883 174 1396
Fragments arranged by size:
1883 1396 1254 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2430 4534 4721 1700
Size: 110 620 2104 187 2869
Fragments arranged by size:
2869 2104 620 187 110
BsrGI T'GTAC_A
Cuts at: 96 2649 96
Size: 2553 3337
BssHII G'CGCG_C
Cuts at: 1925 2113 1925
Size: 188 5702
BssSI C'ACGA_G
Cuts at: 2873 3950 4962 2873
Size: 1077 1012 3801
Fragments arranged by size:
3801 1077 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2504 794
Size: 1710 4180
BstBI TT'CG_AA
Cuts at: 4694 4694
Size: 5890
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5890
BstXI CCAn_nnnn'nTGG
Cuts at: 2515 2515
Size: 5890
BstYI r'GATC_y
Cuts at: 1972 2598 3220 3587 3972 3989 5441 5527 5539
Size: 626 622 367 385 17 1452 86 12
Cuts at: 5539 5885 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 626 622 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2678 2678
Size: 5890
BtgI C'CryG_G
Cuts at: 513 1065 2004 2577 513
Size: 552 939 573 3826
Fragments arranged by size:
3826 939 573 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2688 487
Size: 683 56 1462 3689
Fragments arranged by size:
3689 1462 683 56
BtsI GCAGTG_nn'
Cuts at: 3406 4200 4220 3406
Size: 794 20 5076
Fragments arranged by size:
5076 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5890
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5855
DraI TTT'AAA
Cuts at: 3194 3194
Size: 5890
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4897 809
Size: 507 3581 1802
Fragments arranged by size:
3581 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2010 2028 2058
Size: 54 1425 50 275 18 180 18 30
Cuts at: 2058 2456 3337 4226 5855 8
Size: 398 881 889 1629 43
Fragments arranged by size:
1629 1425 889 881 398 275 180 54
50 43 30 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 2010 3337 1537
Size: 275 198 1327 4090
Fragments arranged by size:
4090 1327 275 198
EarI CTCTTCn'nnn_
Cuts at: 1353 2255 3820 1353
Size: 902 1565 3423
Fragments arranged by size:
3423 1565 902
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4477 4851 4997 387
Size: 1064 138 2888 374 146 1280
Fragments arranged by size:
2888 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2663 2678 3065 3953 4550 5337 5605
Size: 393 1031 15 387 888 597 787 268
Cuts at: 5605 1239
Size: 1524
Fragments arranged by size:
1524 1031 888 787 597 393 387 268
15
EcoNI CCTnn'n_nnAGG
Cuts at: 2957 2957
Size: 5890
EcoO109I rG'GnC_Cy
Cuts at: 2181 2399 2400 2605 2181
Size: 218 1 205 5466
Fragments arranged by size:
5466 218 205 1
EcoRI G'AATT_C
Cuts at: 3199 3577 3199
Size: 378 5512
FspI TGC'GCA
Cuts at: 4396 4396
Size: 5890
HaeII r_GCGC'y
Cuts at: 1678 2146 2191 5037 1678
Size: 468 45 2846 2531
Fragments arranged by size:
2846 2531 468 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4531 4563 4605 4637 1373
Size: 32 135 32 2959 32 42 32 2626
Fragments arranged by size:
2959 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3234 3480 669
Size: 988 281 1296 246 3079
Fragments arranged by size:
3079 1296 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5890
HpaI GTT'AAC
Cuts at: 3480 3480
Size: 5890
KasI G'GCGC_C
Cuts at: 2187 2187
Size: 5890
KpnI G_GTAC'C
Cuts at: 2590 3215 2590
Size: 625 5265
MfeI C'AATT_G
Cuts at: 3489 3489
Size: 5890
MluI A'CGCG_T
Cuts at: 5766 5766
Size: 5890
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5004 5188 1305
Size: 3699 184 2007
Fragments arranged by size:
3699 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2060 10
Size: 54 1425 343 228 3840
Fragments arranged by size:
3840 1425 343 228 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2513 2537 2789 2847 3848 4207 4366
Size: 648 1347 24 252 58 1001 359 159
Cuts at: 4366 518
Size: 2042
Fragments arranged by size:
2042 1347 1001 648 359 252 159 58
24
MspA1I CmG'CkG
Cuts at: 1606 2024 2256 2407 3166 3306 3983 5131 5376
Size: 418 232 151 759 140 677 1148 245
Cuts at: 5376 1606
Size: 2120
Fragments arranged by size:
2120 1148 759 677 418 245 232 151
140
NaeI GCC'GGC
Cuts at: 2432 2432
Size: 5890
NarI GG'CG_CC
Cuts at: 2188 2188
Size: 5890
NcoI C'CATG_G
Cuts at: 513 1065 2004 2577 513
Size: 552 939 573 3826
Fragments arranged by size:
3826 939 573 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5890
NgoMIV G'CCGG_C
Cuts at: 2430 2430
Size: 5890
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5890
NotI GC'GGCC_GC
Cuts at: 3337 3337
Size: 5890
NspI r_CATG'y
Cuts at: 1132 1229 3248 4793 1132
Size: 97 2019 1545 2229
Fragments arranged by size:
2229 2019 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5890
PciI A'CATG_T
Cuts at: 4789 4789
Size: 5890
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5890
PfoI T'CCnGG_A
Cuts at: 2265 2601 2733 2265
Size: 336 132 5422
Fragments arranged by size:
5422 336 132
PmeI GTTT'AAAC
Cuts at: 3194 3194
Size: 5890
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3959 3991 5472 5504 3959
Size: 32 1481 32 4345
Fragments arranged by size:
4345 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2605 2605
Size: 5890
PshAI GACnn'nnGTC
Cuts at: 1613 3173 1613
Size: 1560 4330
PsiI TTA'TAA
Cuts at: 2814 3460 2814
Size: 646 5244
PspOMI G'GGCC_C
Cuts at: 2399 2399
Size: 5890
PstI C_TGCA'G
Cuts at: 830 3242 830
Size: 2412 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5890
PvuII CAG'CTG
Cuts at: 2024 2407 3166 2024
Size: 383 759 4748
Fragments arranged by size:
4748 759 383
SalI G'TCGA_C
Cuts at: 1936 3232 1936
Size: 1296 4594
SapI GCTCTTCn'nnn_
Cuts at: 2255 2255
Size: 5890
SbfI CC_TGCA'GG
Cuts at: 3242 3242
Size: 5890
ScaI AGT'ACT
Cuts at: 2171 4138 5850 2171
Size: 1967 1712 2211
Fragments arranged by size:
2211 1967 1712
SfcI C'TryA_G
Cuts at: 826 1045 2818 3238 4373 5054 5245 826
Size: 219 1773 420 1135 681 191 1471
Fragments arranged by size:
1773 1471 1135 681 420 219 191
SfoI GGC'GCC
Cuts at: 2189 2189
Size: 5890
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5890
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5890
SmaI CCC'GGG
Cuts at: 1872 2300 3217 1872
Size: 428 917 4545
Fragments arranged by size:
4545 917 428
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2469 2919 3994 4895 5157 820
Size: 197 926 526 450 1075 901 262 1553
Fragments arranged by size:
1553 1075 926 901 526 450 262 197
SnaBI TAC'GTA
Cuts at: 493 3681 493
Size: 3188 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5890
SphI G_CATG'C
Cuts at: 1132 3248 1132
Size: 2116 3774
SspI AAT'ATT
Cuts at: 5 52 3814 5
Size: 47 3762 2081
Fragments arranged by size:
3762 2081 47
StuI AGG'CCT
Cuts at: 1517 2235 1517
Size: 718 5172
StyI C'CwwG_G
Cuts at: 513 1065 2004 2550 2577 3329 513
Size: 552 939 546 27 752 3074
Fragments arranged by size:
3074 939 752 552 546 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3917 3934 4087 4272 4717 325
Size: 1255 2337 17 153 185 445 1498
Fragments arranged by size:
2337 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2169 2442 2649
Size: 274 80 33 51 1443 192 273 207
Cuts at: 2649 2802 4136 5848 96
Size: 153 1334 1712 138
Fragments arranged by size:
1712 1443 1334 274 273 207 192 153
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3170 3202 5850 5882 3170
Size: 32 2648 32 3178
Fragments arranged by size:
3178 2648 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5890
XbaI T'CTAG_A
Cuts at: 3226 3226
Size: 5890
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2567 2567
Size: 5890
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5890
XmaI C'CCGG_G
Cuts at: 1870 2298 3215 1870
Size: 428 917 4545
Fragments arranged by size:
4545 917 428
XmnI GAAnn'nnTTC
Cuts at: 4019 4019
Size: 5890
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3698 276
Size: 53 83 186 936 2164 2468
Fragments arranged by size:
2468 2164 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I DraIII
EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI
PmlI PspXI PsrI RsrII SacI SacII SanDI SexAI
SfiI SrfI SwaI