Restriction map for
pFN21AE0492
(Circular) (Six-base) MAPSORT of: pFN21AE0492.seq Check: 8932 from: 1 to: 5545
pFN21AE0492 5545 bp
With 182 enzymes: SgfI *
October 20, 2009 10:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3355 278
Size: 53 83 186 936 1819 2468
Fragments arranged by size:
2468 1819 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2888 1937
Size: 951 4594
Acc65I G'GTAC_C
Cuts at: 2866 2866
Size: 5545
AclI AA'CG_TT
Cuts at: 1563 3672 4045 1563
Size: 2109 373 3063
Fragments arranged by size:
3063 2109 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2303 3608 4992 5260 1632
Size: 671 1305 1384 268 1917
Fragments arranged by size:
1917 1384 1305 671 268
AfeI AGC'GCT
Cuts at: 2176 2176
Size: 5545
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5348
AflIII A'CryG_T
Cuts at: 1203 4444 5421 1203
Size: 3241 977 1327
Fragments arranged by size:
3241 1327 977
AgeI A'CCGG_T
Cuts at: 4376 4376
Size: 5545
AhdI GACnn_n'nnGTC
Cuts at: 2269 4274 2269
Size: 2005 3540
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5545
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5545
AlwNI CAG_nnn'CTG
Cuts at: 2140 2158 3288 4860 2140
Size: 18 1130 1572 2825
Fragments arranged by size:
2825 1572 1130 18
ApaI G_GGCC'C
Cuts at: 2050 2050
Size: 5545
ApaLI G'TGCA_C
Cuts at: 3602 4758 3602
Size: 1156 4389
ApoI r'AATT_y
Cuts at: 1493 2854 3080 3232 5341 1493
Size: 1361 226 152 2109 1697
Fragments arranged by size:
2109 1697 1361 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4099 160
Size: 872 3067 1606
Fragments arranged by size:
3067 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5545
AvaI C'yCGr_G
Cuts at: 1870 1943 2308 2329 2870 1870
Size: 73 365 21 541 4545
Fragments arranged by size:
4545 541 365 73 21
AvrII C'CTAG_G
Cuts at: 2191 2191
Size: 5545
BamHI G'GATC_C
Cuts at: 2875 3242 2875
Size: 367 5178
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2757 2866 4321 618
Size: 325 217 627 970 109 1455 1842
Fragments arranged by size:
1842 1455 970 627 325 217 109
BanII G_rGCy'C
Cuts at: 1391 2050 2297 2344 2386 2837 2864 1391
Size: 659 247 47 42 451 27 4072
Fragments arranged by size:
4072 659 451 247 47 42 27
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2509 2578 2741 928
Size: 971 610 69 163 3732
Fragments arranged by size:
3732 971 610 163 69
BbvCI CC'TCA_GC
Cuts at: 2646 2646
Size: 5545
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3736 3770 1903
Size: 34 1799 34 3678
Fragments arranged by size:
3678 1799 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3437 4647 3437
Size: 1210 4335
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5545
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4156 136
Size: 107 122 71 631 605 2484 1525
Fragments arranged by size:
2484 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5540 5540
Size: 5545
BlpI GC'TnA_GC
Cuts at: 2343 2962 2343
Size: 619 4926
Bme1580I G_kGCm'C
Cuts at: 1790 2050 3606 4762 1790
Size: 260 1556 1156 2573
Fragments arranged by size:
2573 1556 1156 260
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5545
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3276 4234 442
Size: 416 53 83 399 24 1859 958 1753
Fragments arranged by size:
1859 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5545
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4205 1239
Size: 2966 2579
Bpu10I CC'TnA_GC
Cuts at: 2385 2646 2385
Size: 261 5284
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2163 3670 4535 4833 2163
Size: 1507 865 298 2875
Fragments arranged by size:
2875 1507 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2485 4208 882
Size: 617 986 1723 2219
Fragments arranged by size:
2219 1723 986 617
BsaAI yAC'GTr
Cuts at: 493 1204 3336 493
Size: 711 2132 2702
Fragments arranged by size:
2702 2132 711
BsaBI GATnn'nnATC
Cuts at: 5539 5539
Size: 5545
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3352 3734 4369 275
Size: 53 83 186 936 1819 382 635 1451
Fragments arranged by size:
1819 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3978 4376 4650 4797 5208 3978
Size: 398 274 147 411 4315
Fragments arranged by size:
4315 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5320
Fragments arranged by size:
5320 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2296 2410 2501 2569 2663 1186
Size: 1110 114 91 68 94 4068
Fragments arranged by size:
4068 1110 114 94 91 68
BseYI C'CCAG_C
Cuts at: 2011 2372 2549 4748 5244 2011
Size: 361 177 2199 496 2312
Fragments arranged by size:
2312 2199 496 361 177
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4972
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2995 3756 4784 1540
Size: 275 186 994 761 1028 2301
Fragments arranged by size:
2301 1028 994 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2402 2837 3606 3691 4762 2402
Size: 435 769 85 1071 3185
Fragments arranged by size:
3185 1071 769 435 85
BsmI GAATG_Cn'
Cuts at: 2293 3056 3149 2293
Size: 763 93 4689
Fragments arranged by size:
4689 763 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2050 2297 2344 2386 2402 2837 2864
Size: 399 260 247 47 42 16 435 27
Cuts at: 2864 3606 3691 4762 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 435 399 260 247 85
47 42 27 16
BspEI T'CCGG_A
Cuts at: 5208 5208
Size: 5545
BspHI T'CATG_A
Cuts at: 2664 3434 5164 2664
Size: 770 1730 3045
Fragments arranged by size:
3045 1730 770
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2015 2900 844
Size: 1171 885 3489
Fragments arranged by size:
3489 1171 885
BsrBI CCG'CTC
Cuts at: 1394 1823 3432 1394
Size: 429 1609 3507
Fragments arranged by size:
3507 1609 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2467 4040 4214 65
Size: 1183 1219 1573 174 1396
Fragments arranged by size:
1573 1396 1219 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4189 4376 1700
Size: 110 2379 187 2869
Fragments arranged by size:
2869 2379 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5545
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5545
BssSI C'ACGA_G
Cuts at: 3605 4617 3605
Size: 1012 4533
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5545
BstBI TT'CG_AA
Cuts at: 4349 4349
Size: 5545
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5545
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2453 2011
Size: 442 5103
BstYI r'GATC_y
Cuts at: 1972 2875 3242 3627 3644 5096 5182 5194 5540
Size: 903 367 385 17 1452 86 12 346
Cuts at: 5540 1972
Size: 1977
Fragments arranged by size:
1977 1452 903 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2017 513
Size: 552 939 13 4041
Fragments arranged by size:
4041 939 552 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4806
Fragments arranged by size:
4806 683 56
BtsI GCAGTG_nn'
Cuts at: 2204 3061 3855 3875 2204
Size: 857 794 20 3874
Fragments arranged by size:
3874 857 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5545
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5510
DraI TTT'AAA
Cuts at: 2849 2849
Size: 5545
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2477 4552 809
Size: 507 1161 2075 1802
Fragments arranged by size:
2075 1802 1161 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2992 3881 5510
Size: 54 1425 50 275 18 1162 889 1629
Cuts at: 5510 8
Size: 43
Fragments arranged by size:
1629 1425 1162 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2992 1537
Size: 275 1180 4090
Fragments arranged by size:
4090 1180 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2796 3475 1353
Size: 1443 679 3423
Fragments arranged by size:
3423 1443 679
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4132 4506 4652 387
Size: 1064 138 2543 374 146 1280
Fragments arranged by size:
2543 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2835 2835
Size: 5545
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2303 3608 4205 4992 5260 1239
Size: 393 671 1305 597 787 268 1524
Fragments arranged by size:
1524 1305 787 671 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2666 2666
Size: 5545
EcoO109I rG'GnC_Cy
Cuts at: 2046 2277 2556 2046
Size: 231 279 5035
Fragments arranged by size:
5035 279 231
EcoRI G'AATT_C
Cuts at: 2854 3232 2854
Size: 378 5167
FspI TGC'GCA
Cuts at: 4051 4051
Size: 5545
HaeII r_GCGC'y
Cuts at: 1678 2178 4692 1678
Size: 500 2514 2531
Fragments arranged by size:
2531 2514 500
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2206 2238 2255 2287 2548
Size: 32 135 32 634 32 17 32 261
Cuts at: 2548 2580 2728 2760 4186 4218 4260 4292 1373
Size: 32 148 32 1426 32 42 32 2626
Fragments arranged by size:
2626 1426 634 261 148 135 42 32
32 32 32 32 32 32 32 17
HincII GTy'rAC
Cuts at: 669 1657 1938 2889 3135 669
Size: 988 281 951 246 3079
Fragments arranged by size:
3079 988 951 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5545
HpaI GTT'AAC
Cuts at: 3135 3135
Size: 5545
KpnI G_GTAC'C
Cuts at: 2870 2870
Size: 5545
MfeI C'AATT_G
Cuts at: 3144 3144
Size: 5545
MluI A'CGCG_T
Cuts at: 5421 5421
Size: 5545
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4659 4843 1305
Size: 3354 184 2007
Fragments arranged by size:
3354 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3723
Fragments arranged by size:
3723 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2055 2724 3503 3862 4021 518
Size: 648 889 669 779 359 159 2042
Fragments arranged by size:
2042 889 779 669 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2476 2961 3638 4786 5031 1606
Size: 870 485 677 1148 245 2120
Fragments arranged by size:
2120 1148 870 677 485 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4054
Fragments arranged by size:
4054 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5545
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5545
NotI GC'GGCC_GC
Cuts at: 2992 2992
Size: 5545
NspI r_CATG'y
Cuts at: 1132 1229 2620 2903 4448 1132
Size: 97 1391 283 1545 2229
Fragments arranged by size:
2229 1545 1391 283 97
PasI CC'CwG_GG
Cuts at: 1348 2164 1348
Size: 816 4729
PciI A'CATG_T
Cuts at: 4444 4444
Size: 5545
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5545
PmeI GTTT'AAAC
Cuts at: 2849 2849
Size: 5545
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3614 3646 5127 5159 3614
Size: 32 1481 32 4000
Fragments arranged by size:
4000 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2277 2556 2277
Size: 279 5266
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5545
PsiI TTA'TAA
Cuts at: 3115 3115
Size: 5545
PspOMI G'GGCC_C
Cuts at: 2046 2046
Size: 5545
PstI C_TGCA'G
Cuts at: 830 2897 830
Size: 2067 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5545
PvuII CAG'CTG
Cuts at: 2476 2476
Size: 5545
SacI G_AGCT'C
Cuts at: 2837 2837
Size: 5545
SalI G'TCGA_C
Cuts at: 1936 2887 1936
Size: 951 4594
SbfI CC_TGCA'GG
Cuts at: 2897 2897
Size: 5545
ScaI AGT'ACT
Cuts at: 3793 5505 3793
Size: 1712 3833
SfcI C'TryA_G
Cuts at: 826 1045 2101 2893 4028 4709 4900 826
Size: 219 1056 792 1135 681 191 1471
Fragments arranged by size:
1471 1135 1056 792 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5545
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5545
SmaI CCC'GGG
Cuts at: 1872 2872 1872
Size: 1000 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2178 2308 3649 4550 4812 820
Size: 197 926 235 130 1341 901 262 1553
Fragments arranged by size:
1553 1341 926 901 262 235 197 130
SnaBI TAC'GTA
Cuts at: 493 3336 493
Size: 2843 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5545
SphI G_CATG'C
Cuts at: 1132 2903 1132
Size: 1771 3774
SspI AAT'ATT
Cuts at: 5 52 3469 5
Size: 47 3417 2081
Fragments arranged by size:
3417 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5545
StyI C'CwwG_G
Cuts at: 513 1065 2004 2109 2191 2984 513
Size: 552 939 105 82 793 3074
Fragments arranged by size:
3074 939 793 552 105 82
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3572 3589 3742 3927 4372 325
Size: 1255 1992 17 153 185 445 1498
Fragments arranged by size:
1992 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3791 5503 96
Size: 274 80 33 51 1443 1814 1712 138
Fragments arranged by size:
1814 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5505 5537 5505
Size: 32 5513
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5545
XbaI T'CTAG_A
Cuts at: 2881 2881
Size: 5545
XhoI C'TCGA_G
Cuts at: 1943 2308 1943
Size: 365 5180
XmaI C'CCGG_G
Cuts at: 1870 2870 1870
Size: 1000 4545
XmnI GAAnn'nnTTC
Cuts at: 3674 3674
Size: 5545
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3353 276
Size: 53 83 186 936 1819 2468
Fragments arranged by size:
2468 1819 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI BaeI BbeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI