Restriction map for
pFN21AE0491
(Circular) (Six-base) MAPSORT of: pFN21AE0491.seq Check: 6976 from: 1 to: 5674
pFN21AE0491 5674 bp
With 182 enzymes: SgfI *
October 20, 2009 10:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3484 278
Size: 53 83 186 936 1948 2468
Fragments arranged by size:
2468 1948 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3017 1937
Size: 1080 4594
Acc65I G'GTAC_C
Cuts at: 2995 2995
Size: 5674
AclI AA'CG_TT
Cuts at: 1563 3801 4174 1563
Size: 2238 373 3063
Fragments arranged by size:
3063 2238 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2050 2858 3737 5121 5389 1632
Size: 418 808 879 1384 268 1917
Fragments arranged by size:
1917 1384 879 808 418 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5477
AflIII A'CryG_T
Cuts at: 1203 2601 4573 5550 1203
Size: 1398 1972 977 1327
Fragments arranged by size:
1972 1398 1327 977
AgeI A'CCGG_T
Cuts at: 4505 4505
Size: 5674
AhdI GACnn_n'nnGTC
Cuts at: 4403 4403
Size: 5674
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5674
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5674
AlwNI CAG_nnn'CTG
Cuts at: 3417 4989 3417
Size: 1572 4102
ApaI G_GGCC'C
Cuts at: 2557 2557
Size: 5674
ApaLI G'TGCA_C
Cuts at: 2597 3731 4887 2597
Size: 1134 1156 3384
Fragments arranged by size:
3384 1156 1134
ApoI r'AATT_y
Cuts at: 1493 2218 2983 3209 3361 5470 1493
Size: 725 765 226 152 2109 1697
Fragments arranged by size:
2109 1697 765 725 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4228 160
Size: 872 3196 1606
Fragments arranged by size:
3196 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5674
AvaI C'yCGr_G
Cuts at: 1870 1943 2999 1870
Size: 73 1056 4545
Fragments arranged by size:
4545 1056 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2893 2926 2893
Size: 33 5641
BamHI G'GATC_C
Cuts at: 3004 3371 3004
Size: 367 5307
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2995 4450 618
Size: 325 217 627 1208 1455 1842
Fragments arranged by size:
1842 1455 1208 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2557 2742 2993 1391
Size: 1166 185 251 4072
Fragments arranged by size:
4072 1166 251 185
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4703
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2410 2444 3865 3899 1903
Size: 34 473 34 1421 34 3678
Fragments arranged by size:
3678 1421 473 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2921 3566 4776 2921
Size: 645 1210 3819
Fragments arranged by size:
3819 1210 645
BclI T'GATC_A
Cuts at: 1548 2245 1548
Size: 697 4977
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2309 4285 136
Size: 107 122 71 631 605 637 1976 1525
Fragments arranged by size:
1976 1525 637 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2805 5669 2805
Size: 2864 2810
BlpI GC'TnA_GC
Cuts at: 2822 3091 2822
Size: 269 5405
Bme1580I G_kGCm'C
Cuts at: 1790 2557 2601 3735 4891 1790
Size: 767 44 1134 1156 2573
Fragments arranged by size:
2573 1156 1134 767 44
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5674
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3405 4363 442
Size: 416 53 83 399 24 1988 958 1753
Fragments arranged by size:
1988 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5674
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2095 2299 2642 2704 2734 2879 4334 1239
Size: 856 204 343 62 30 145 1455 2579
Fragments arranged by size:
2579 1455 856 343 204 145 62 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3799 4664 4962 3799
Size: 865 298 4511
Fragments arranged by size:
4511 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2484 4337 882
Size: 617 985 1853 2219
Fragments arranged by size:
2219 1853 985 617
BsaAI yAC'GTr
Cuts at: 493 1204 3465 493
Size: 711 2261 2702
Fragments arranged by size:
2702 2261 711
BsaBI GATnn'nnATC
Cuts at: 5668 5668
Size: 5674
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2757 3481 3863 4498
Size: 53 83 186 936 1224 724 382 635
Cuts at: 4498 275
Size: 1451
Fragments arranged by size:
1451 1224 936 724 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2186 4107 4505 4779 4926 5337 2186
Size: 1921 398 274 147 411 2523
Fragments arranged by size:
2523 1921 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5449
Fragments arranged by size:
5449 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5674
BseYI C'CCAG_C
Cuts at: 2684 4877 5373 2684
Size: 2193 496 2985
Fragments arranged by size:
2985 2193 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5101
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2614 3124 3885 4913 1540
Size: 275 186 613 510 761 1028 2301
Fragments arranged by size:
2301 1028 761 613 510 275 186
BsiHKAI G_wGCw'C
Cuts at: 2601 3735 3820 4891 2601
Size: 1134 85 1071 3384
Fragments arranged by size:
3384 1134 1071 85
BsmI GAATG_Cn'
Cuts at: 3185 3278 3185
Size: 93 5581
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2557 2601 2742 2993 3735 3820 4891
Size: 399 767 44 141 251 742 85 1071
Cuts at: 4891 1391
Size: 2174
Fragments arranged by size:
2174 1071 767 742 399 251 141 85
44
BspEI T'CCGG_A
Cuts at: 2186 5337 2186
Size: 3151 2523
BspHI T'CATG_A
Cuts at: 3563 5293 3563
Size: 1730 3944
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2210 2429 3029 844
Size: 1366 219 600 3489
Fragments arranged by size:
3489 1366 600 219
BsrBI CCG'CTC
Cuts at: 1394 1823 2718 3561 1394
Size: 429 895 843 3507
Fragments arranged by size:
3507 895 843 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4169 4343 65
Size: 1183 2921 174 1396
Fragments arranged by size:
2921 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2417 2613 4318 4505 1700
Size: 110 607 196 1705 187 2869
Fragments arranged by size:
2869 1705 607 196 187 110
BsrGI T'GTAC_A
Cuts at: 96 2469 2814 96
Size: 2373 345 2956
Fragments arranged by size:
2956 2373 345
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5674
BssSI C'ACGA_G
Cuts at: 3734 4746 3734
Size: 1012 4662
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5674
BstBI TT'CG_AA
Cuts at: 4478 4478
Size: 5674
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5674
BstXI CCAn_nnnn'nTGG
Cuts at: 2086 2086
Size: 5674
BstYI r'GATC_y
Cuts at: 1972 2805 3004 3371 3756 3773 5225 5311 5323
Size: 833 199 367 385 17 1452 86 12
Cuts at: 5323 5669 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 833 385 367 346 199 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2634 2646 2733 2766 2799 513
Size: 552 939 630 12 87 33 33 3388
Fragments arranged by size:
3388 939 630 552 87 33 33 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2353 2554 487
Size: 683 56 1127 201 3607
Fragments arranged by size:
3607 1127 683 201 56
BtsI GCAGTG_nn'
Cuts at: 3190 3984 4004 3190
Size: 794 20 4860
Fragments arranged by size:
4860 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5674
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5639
DraI TTT'AAA
Cuts at: 2978 2978
Size: 5674
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4681 809
Size: 507 3365 1802
Fragments arranged by size:
3365 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3121 4010 5639
Size: 54 1425 50 275 18 1291 889 1629
Cuts at: 5639 8
Size: 43
Fragments arranged by size:
1629 1425 1291 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3121 1537
Size: 275 1309 4090
Fragments arranged by size:
4090 1309 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3604 1353
Size: 2251 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4261 4635 4781 387
Size: 1064 138 2672 374 146 1280
Fragments arranged by size:
2672 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2050 2095 2299 2642 2704 2734 2858
Size: 393 418 45 204 343 62 30 124
Cuts at: 2858 2879 3737 4334 5121 5389 1239
Size: 21 858 597 787 268 1524
Fragments arranged by size:
1524 858 787 597 418 393 343 268
204 124 62 45 30 21
EcoO109I rG'GnC_Cy
Cuts at: 2310 2553 2554 2830 2310
Size: 243 1 276 5154
Fragments arranged by size:
5154 276 243 1
EcoRI G'AATT_C
Cuts at: 2983 3361 2983
Size: 378 5296
FspI TGC'GCA
Cuts at: 4180 4180
Size: 5674
HaeII r_GCGC'y
Cuts at: 1678 2710 4821 1678
Size: 1032 2111 2531
Fragments arranged by size:
2531 2111 1032
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2388 2420 4315 4347 4389
Size: 32 135 32 816 32 1895 32 42
Cuts at: 4389 4421 1373
Size: 32 2626
Fragments arranged by size:
2626 1895 816 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3018 3264 669
Size: 988 281 1080 246 3079
Fragments arranged by size:
3079 1080 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5674
HpaI GTT'AAC
Cuts at: 3264 3264
Size: 5674
KpnI G_GTAC'C
Cuts at: 2999 2999
Size: 5674
MfeI C'AATT_G
Cuts at: 3273 3273
Size: 5674
MluI A'CGCG_T
Cuts at: 5550 5550
Size: 5674
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2898 2967 4788 4972 1305
Size: 1593 69 1821 184 2007
Fragments arranged by size:
2007 1821 1593 184 69
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3852
Fragments arranged by size:
3852 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2084 2465 3632 3991 4150 518
Size: 648 918 381 1167 359 159 2042
Fragments arranged by size:
2042 1167 918 648 381 359 159
MspA1I CmG'CkG
Cuts at: 1606 2626 3090 3767 4915 5160 1606
Size: 1020 464 677 1148 245 2120
Fragments arranged by size:
2120 1148 1020 677 464 245
NaeI GCC'GGC
Cuts at: 2419 2419
Size: 5674
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4183
Fragments arranged by size:
4183 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5674
NgoMIV G'CCGG_C
Cuts at: 2417 2417
Size: 5674
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5674
NotI GC'GGCC_GC
Cuts at: 3121 3121
Size: 5674
NspI r_CATG'y
Cuts at: 1132 1229 2605 3032 4577 1132
Size: 97 1376 427 1545 2229
Fragments arranged by size:
2229 1545 1376 427 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5674
PciI A'CATG_T
Cuts at: 2601 4573 2601
Size: 1972 3702
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5674
PmeI GTTT'AAAC
Cuts at: 2978 2978
Size: 5674
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2915 2947 3743 3775 5256 5288 2915
Size: 32 796 32 1481 32 3301
Fragments arranged by size:
3301 1481 796 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2830 2830
Size: 5674
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5674
PsiI TTA'TAA
Cuts at: 3244 3244
Size: 5674
PspOMI G'GGCC_C
Cuts at: 2553 2553
Size: 5674
PstI C_TGCA'G
Cuts at: 830 2440 3026 830
Size: 1610 586 3478
Fragments arranged by size:
3478 1610 586
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5674
PvuII CAG'CTG
Cuts at: 2626 2626
Size: 5674
SalI G'TCGA_C
Cuts at: 1936 3016 1936
Size: 1080 4594
SanDI GG'GwC_CC
Cuts at: 2830 2830
Size: 5674
SbfI CC_TGCA'GG
Cuts at: 3026 3026
Size: 5674
ScaI AGT'ACT
Cuts at: 3922 5634 3922
Size: 1712 3962
SfcI C'TryA_G
Cuts at: 826 1045 2436 3022 4157 4838 5029 826
Size: 219 1391 586 1135 681 191 1471
Fragments arranged by size:
1471 1391 1135 681 586 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2309 2309
Size: 5674
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5674
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5674
SmaI CCC'GGG
Cuts at: 1872 3001 1872
Size: 1129 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3778 4679 4941 820
Size: 197 926 1835 901 262 1553
Fragments arranged by size:
1835 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3465 493
Size: 2972 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5674
SphI G_CATG'C
Cuts at: 1132 3032 1132
Size: 1900 3774
SspI AAT'ATT
Cuts at: 5 52 2291 3598 5
Size: 47 2239 1307 2081
Fragments arranged by size:
2239 2081 1307 47
StuI AGG'CCT
Cuts at: 1517 2663 1517
Size: 1146 4528
StyI C'CwwG_G
Cuts at: 513 1065 2004 3113 513
Size: 552 939 1109 3074
Fragments arranged by size:
3074 1109 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2345 3701 3718 3871 4056 4501 325
Size: 1255 765 1356 17 153 185 445 1498
Fragments arranged by size:
1498 1356 1255 765 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2469 2814 3920
Size: 274 80 33 51 1443 492 345 1106
Cuts at: 3920 5632 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1106 492 345 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5634 5666 5634
Size: 32 5642
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5674
XbaI T'CTAG_A
Cuts at: 3010 3010
Size: 5674
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5674
XmaI C'CCGG_G
Cuts at: 1870 2999 1870
Size: 1129 4545
XmnI GAAnn'nnTTC
Cuts at: 3803 3803
Size: 5674
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3482 276
Size: 53 83 186 936 1948 2468
Fragments arranged by size:
2468 1948 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI
SacII SapI SexAI SfoI SrfI SwaI XcmI