Restriction map for
pFN21AE0489
(Circular) (Six-base) MAPSORT of: pFN21AE0489.seq Check: 4722 from: 1 to: 5212
pFN21AE0489 5212 bp
With 182 enzymes: SgfI *
October 20, 2009 10:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3022 278
Size: 53 83 186 936 1486 2468
Fragments arranged by size:
2468 1486 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2555 1937
Size: 618 4594
Acc65I G'GTAC_C
Cuts at: 2382 2533 2382
Size: 151 5061
AclI AA'CG_TT
Cuts at: 1563 3339 3712 1563
Size: 1776 373 3063
Fragments arranged by size:
3063 1776 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3275 4659 4927 1632
Size: 1643 1384 268 1917
Fragments arranged by size:
1917 1643 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5015
AflIII A'CryG_T
Cuts at: 1203 4111 5088 1203
Size: 2908 977 1327
Fragments arranged by size:
2908 1327 977
AgeI A'CCGG_T
Cuts at: 4043 4043
Size: 5212
AhdI GACnn_n'nnGTC
Cuts at: 3941 3941
Size: 5212
AleI CACnn'nnGTG
Cuts at: 1166 2372 1166
Size: 1206 4006
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5212
AlwNI CAG_nnn'CTG
Cuts at: 2955 4527 2955
Size: 1572 3640
ApaI G_GGCC'C
Cuts at: 2156 2156
Size: 5212
ApaLI G'TGCA_C
Cuts at: 3269 4425 3269
Size: 1156 4056
ApoI r'AATT_y
Cuts at: 1493 2521 2747 2899 5008 1493
Size: 1028 226 152 2109 1697
Fragments arranged by size:
2109 1697 1028 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3766 160
Size: 872 2734 1606
Fragments arranged by size:
2734 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5212
AvaI C'yCGr_G
Cuts at: 1870 1943 2537 1870
Size: 73 594 4545
Fragments arranged by size:
4545 594 73
BamHI G'GATC_C
Cuts at: 2542 2909 2542
Size: 367 4845
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2020 2044 2130 2306 2382
Size: 325 217 627 233 24 86 176 76
Cuts at: 2382 2533 3988 618
Size: 151 1455 1842
Fragments arranged by size:
1842 1455 627 325 233 217 176 151
86 76 24
BanII G_rGCy'C
Cuts at: 1391 2072 2156 2396 2531 1391
Size: 681 84 240 135 4072
Fragments arranged by size:
4072 681 240 135 84
BbeI G_GCGC'C
Cuts at: 2048 2048
Size: 5212
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4241
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3403 3437 1903
Size: 34 1466 34 3678
Fragments arranged by size:
3678 1466 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3104 4314 3104
Size: 1210 4002
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5212
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2128 2435 3823
Size: 107 122 71 631 605 456 307 1388
Cuts at: 3823 136
Size: 1525
Fragments arranged by size:
1525 1388 631 605 456 307 122 107
71
BglII A'GATC_T
Cuts at: 5207 5207
Size: 5212
BlpI GC'TnA_GC
Cuts at: 2629 2629
Size: 5212
Bme1580I G_kGCm'C
Cuts at: 1790 2025 2156 2168 2311 3273 4429 1790
Size: 235 131 12 143 962 1156 2573
Fragments arranged by size:
2573 1156 962 235 143 131 12
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5212
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2152 2271 2290
Size: 416 53 83 399 24 735 119 19
Cuts at: 2290 2943 3901 442
Size: 653 958 1753
Fragments arranged by size:
1753 958 735 653 416 399 119 83
53 24 19
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5212
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3872 1239
Size: 2633 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3337 4202 4500 3337
Size: 865 298 4049
Fragments arranged by size:
4049 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2099 3875 882
Size: 617 600 1776 2219
Fragments arranged by size:
2219 1776 617 600
BsaAI yAC'GTr
Cuts at: 493 1204 3003 493
Size: 711 1799 2702
Fragments arranged by size:
2702 1799 711
BsaBI GATnn'nnATC
Cuts at: 5206 5206
Size: 5212
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2045 3019 3401 4036
Size: 53 83 186 936 512 974 382 635
Cuts at: 4036 275
Size: 1451
Fragments arranged by size:
1451 974 936 635 512 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3645 4043 4317 4464 4875 3645
Size: 398 274 147 411 3982
Fragments arranged by size:
3982 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4987
Fragments arranged by size:
4987 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5212
BseYI C'CCAG_C
Cuts at: 2048 2149 2215 2322 4415 4911 2048
Size: 101 66 107 2093 496 2349
Fragments arranged by size:
2349 2093 496 107 101 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4639
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2662 3423 4451 1540
Size: 275 186 661 761 1028 2301
Fragments arranged by size:
2301 1028 761 661 275 186
BsiHKAI G_wGCw'C
Cuts at: 3273 3358 4429 3273
Size: 85 1071 4056
Fragments arranged by size:
4056 1071 85
BsmI GAATG_Cn'
Cuts at: 2723 2816 2723
Size: 93 5119
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2025 2072 2156 2168 2311 2396 2531
Size: 399 235 47 84 12 143 85 135
Cuts at: 2531 3273 3358 4429 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 235 143 135 85
85 84 47 12
BspEI T'CCGG_A
Cuts at: 4875 4875
Size: 5212
BspHI T'CATG_A
Cuts at: 3101 4831 3101
Size: 1730 3482
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2567 844
Size: 1723 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3099 1394
Size: 429 1276 3507
Fragments arranged by size:
3507 1276 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3707 3881 65
Size: 1183 2459 174 1396
Fragments arranged by size:
2459 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2108 3856 4043 1700
Size: 110 298 1748 187 2869
Fragments arranged by size:
2869 1748 298 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5212
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5212
BssSI C'ACGA_G
Cuts at: 2125 3272 4284 2125
Size: 1147 1012 3053
Fragments arranged by size:
3053 1147 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5212
BstBI TT'CG_AA
Cuts at: 4016 4016
Size: 5212
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5212
BstXI CCAn_nnnn'nTGG
Cuts at: 2374 2374
Size: 5212
BstYI r'GATC_y
Cuts at: 1972 2335 2542 2909 3294 3311 4763 4849 4861
Size: 363 207 367 385 17 1452 86 12
Cuts at: 4861 5207 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 363 346 207 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2028 2083 2181 2373 2479 513
Size: 552 939 24 55 98 192 106 3246
Fragments arranged by size:
3246 939 552 192 106 98 55 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4473
Fragments arranged by size:
4473 683 56
BtsI GCAGTG_nn'
Cuts at: 2728 3522 3542 2728
Size: 794 20 4398
Fragments arranged by size:
4398 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5212
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5177
DraI TTT'AAA
Cuts at: 2516 2516
Size: 5212
DraIII CAC_nnn'GTG
Cuts at: 2146 2146
Size: 5212
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4219 809
Size: 507 2903 1802
Fragments arranged by size:
2903 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2400 2659 3548
Size: 54 1425 50 275 18 570 259 889
Cuts at: 3548 5177 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 570 275 259 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2659 1537
Size: 275 847 4090
Fragments arranged by size:
4090 847 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3142 1353
Size: 1789 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3799 4173 4319 387
Size: 1064 138 2210 374 146 1280
Fragments arranged by size:
2210 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3275 3872 4659 4927 1239
Size: 393 1643 597 787 268 1524
Fragments arranged by size:
1643 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2153 2153
Size: 5212
EcoRI G'AATT_C
Cuts at: 2521 2899 2521
Size: 378 4834
FspI TGC'GCA
Cuts at: 3718 3718
Size: 5212
HaeII r_GCGC'y
Cuts at: 1678 2048 4359 1678
Size: 370 2311 2531
Fragments arranged by size:
2531 2311 370
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3853 3885 3927 3959 1373
Size: 32 135 32 2281 32 42 32 2626
Fragments arranged by size:
2626 2281 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2556 2802 669
Size: 988 281 618 246 3079
Fragments arranged by size:
3079 988 618 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5212
HpaI GTT'AAC
Cuts at: 2802 2802
Size: 5212
KasI G'GCGC_C
Cuts at: 2044 2044
Size: 5212
KpnI G_GTAC'C
Cuts at: 2386 2537 2386
Size: 151 5061
MfeI C'AATT_G
Cuts at: 2811 2811
Size: 5212
MluI A'CGCG_T
Cuts at: 5088 5088
Size: 5212
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4326 4510 1305
Size: 3021 184 2007
Fragments arranged by size:
3021 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3390
Fragments arranged by size:
3390 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2372 3170 3529 3688 518
Size: 648 1206 798 359 159 2042
Fragments arranged by size:
2042 1206 798 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2100 2269 2398 2628 3305 4453 4698 1606
Size: 494 169 129 230 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 494 245 230 169 129
NaeI GCC'GGC
Cuts at: 2110 2110
Size: 5212
NarI GG'CG_CC
Cuts at: 2045 2045
Size: 5212
NcoI C'CATG_G
Cuts at: 513 1065 2004 2028 2181 2479 513
Size: 552 939 24 153 298 3246
Fragments arranged by size:
3246 939 552 298 153 24
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5212
NgoMIV G'CCGG_C
Cuts at: 2108 2108
Size: 5212
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5212
NotI GC'GGCC_GC
Cuts at: 2659 2659
Size: 5212
NspI r_CATG'y
Cuts at: 1132 1229 2570 4115 1132
Size: 97 1341 1545 2229
Fragments arranged by size:
2229 1545 1341 97
PasI CC'CwG_GG
Cuts at: 1348 2016 2248 1348
Size: 668 232 4312
Fragments arranged by size:
4312 668 232
PciI A'CATG_T
Cuts at: 4111 4111
Size: 5212
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5212
PmeI GTTT'AAAC
Cuts at: 2516 2516
Size: 5212
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3281 3313 4794 4826 3281
Size: 32 1481 32 3667
Fragments arranged by size:
3667 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5212
PsiI TTA'TAA
Cuts at: 2782 2782
Size: 5212
PspOMI G'GGCC_C
Cuts at: 2152 2152
Size: 5212
PstI C_TGCA'G
Cuts at: 830 2118 2564 830
Size: 1288 446 3478
Fragments arranged by size:
3478 1288 446
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5212
PvuII CAG'CTG
Cuts at: 2269 2269
Size: 5212
SalI G'TCGA_C
Cuts at: 1936 2554 1936
Size: 618 4594
SbfI CC_TGCA'GG
Cuts at: 2564 2564
Size: 5212
ScaI AGT'ACT
Cuts at: 3460 5172 3460
Size: 1712 3500
SexAI A'CCwGG_T
Cuts at: 2199 2385 2199
Size: 186 5026
SfcI C'TryA_G
Cuts at: 826 1045 2114 2560 3695 4376 4567 826
Size: 219 1069 446 1135 681 191 1471
Fragments arranged by size:
1471 1135 1069 681 446 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2435 2435
Size: 5212
SfoI GGC'GCC
Cuts at: 2046 2046
Size: 5212
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5212
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5212
SmaI CCC'GGG
Cuts at: 1872 2539 1872
Size: 667 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3316 4217 4479 820
Size: 197 926 1373 901 262 1553
Fragments arranged by size:
1553 1373 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3003 493
Size: 2510 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5212
SphI G_CATG'C
Cuts at: 1132 2570 1132
Size: 1438 3774
SspI AAT'ATT
Cuts at: 5 52 3136 5
Size: 47 3084 2081
Fragments arranged by size:
3084 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5212
StyI C'CwwG_G
Cuts at: 513 1065 2004 2028 2181 2328 2479 2651 513
Size: 552 939 24 153 147 151 172 3074
Fragments arranged by size:
3074 939 552 172 153 151 147 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2008 2134 3239 3256 3409 3594 4039
Size: 1255 428 126 1105 17 153 185 445
Cuts at: 4039 325
Size: 1498
Fragments arranged by size:
1498 1255 1105 445 428 185 153 126
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3458 5170 96
Size: 274 80 33 51 1443 1481 1712 138
Fragments arranged by size:
1712 1481 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5172 5204 5172
Size: 32 5180
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5212
XbaI T'CTAG_A
Cuts at: 2548 2548
Size: 5212
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2486 2486
Size: 5212
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5212
XmaI C'CCGG_G
Cuts at: 1870 2537 1870
Size: 667 4545
XmnI GAAnn'nnTTC
Cuts at: 3341 3341
Size: 5212
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3020 276
Size: 53 83 186 936 1486 2468
Fragments arranged by size:
2468 1486 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI
EcoRV FalI FseI FspAI NruI NsiI PacI PfoI
PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI
SapI SrfI SwaI