Restriction map for
pFN21AE0488
(Circular) (Six-base) MAPSORT of: pFN21AE0488.seq Check: 7840 from: 1 to: 5812
pFN21AE0488 5812 bp
With 182 enzymes: SgfI *
October 20, 2009 10:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3622 278
Size: 53 83 186 936 2086 2468
Fragments arranged by size:
2468 2086 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3155 1937
Size: 1218 4594
Acc65I G'GTAC_C
Cuts at: 2466 3133 2466
Size: 667 5145
AclI AA'CG_TT
Cuts at: 1563 3939 4312 1563
Size: 2376 373 3063
Fragments arranged by size:
3063 2376 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3875 5259 5527 1632
Size: 2243 1384 268 1917
Fragments arranged by size:
2243 1917 1384 268
AfeI AGC'GCT
Cuts at: 2411 2411
Size: 5812
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5615
AflIII A'CryG_T
Cuts at: 1203 2319 2808 4711 5688 1203
Size: 1116 489 1903 977 1327
Fragments arranged by size:
1903 1327 1116 977 489
AgeI A'CCGG_T
Cuts at: 4643 4643
Size: 5812
AhdI GACnn_n'nnGTC
Cuts at: 2040 4541 2040
Size: 2501 3311
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5812
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2837 1184
Size: 1653 4159
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2571 2603 2571
Size: 32 5780
AlwNI CAG_nnn'CTG
Cuts at: 2380 2733 3555 5127 2380
Size: 353 822 1572 3065
Fragments arranged by size:
3065 1572 822 353
ApaLI G'TGCA_C
Cuts at: 3869 5025 3869
Size: 1156 4656
ApoI r'AATT_y
Cuts at: 1493 3121 3347 3499 5608 1493
Size: 1628 226 152 2109 1697
Fragments arranged by size:
2109 1697 1628 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4366 160
Size: 872 3334 1606
Fragments arranged by size:
3334 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5812
AvaI C'yCGr_G
Cuts at: 1870 1943 2029 2090 2498 2544 3137 1870
Size: 73 86 61 408 46 593 4545
Fragments arranged by size:
4545 593 408 86 73 61 46
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2738 2771 2738
Size: 33 5779
BamHI G'GATC_C
Cuts at: 3142 3509 3142
Size: 367 5445
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2014 2466 3133 4588 618
Size: 325 217 627 227 452 667 1455 1842
Fragments arranged by size:
1842 1455 667 627 452 325 227 217
BanII G_rGCy'C
Cuts at: 1391 2831 2937 3131 1391
Size: 1440 106 194 4072
Fragments arranged by size:
4072 1440 194 106
BbeI G_GCGC'C
Cuts at: 2018 2018
Size: 5812
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2497 928
Size: 971 598 4243
Fragments arranged by size:
4243 971 598
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4003 4037 1903
Size: 34 2066 34 3678
Fragments arranged by size:
3678 2066 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3704 4914 3704
Size: 1210 4602
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5812
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4423 136
Size: 107 122 71 631 605 2751 1525
Fragments arranged by size:
2751 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5807 5807
Size: 5812
BlpI GC'TnA_GC
Cuts at: 2825 3229 2825
Size: 404 5408
Bme1580I G_kGCm'C
Cuts at: 1790 2747 3873 5029 1790
Size: 957 1126 1156 2573
Fragments arranged by size:
2573 1156 1126 957
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5812
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2058 2932 3543
Size: 416 53 83 399 24 641 874 611
Cuts at: 3543 4501 442
Size: 958 1753
Fragments arranged by size:
1753 958 874 641 611 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5812
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2128 4472 1239
Size: 889 2344 2579
Fragments arranged by size:
2579 2344 889
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3937 4802 5100 3937
Size: 865 298 4649
Fragments arranged by size:
4649 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2606 4475 882
Size: 617 1107 1869 2219
Fragments arranged by size:
2219 1869 1107 617
BsaAI yAC'GTr
Cuts at: 493 1204 2236 2320 3603 493
Size: 711 1032 84 1283 2702
Fragments arranged by size:
2702 1283 1032 711 84
BsaBI GATnn'nnATC
Cuts at: 5806 5806
Size: 5812
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2015 3619 4001 4636
Size: 53 83 186 936 482 1604 382 635
Cuts at: 4636 275
Size: 1451
Fragments arranged by size:
1604 1451 936 635 482 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4245 4643 4917 5064 5475 4245
Size: 398 274 147 411 4582
Fragments arranged by size:
4582 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5587
Fragments arranged by size:
5587 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5812
BseYI C'CCAG_C
Cuts at: 2675 2943 5015 5511 2675
Size: 268 2072 496 2976
Fragments arranged by size:
2976 2072 496 268
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2335 1163
Size: 573 599 4640
Fragments arranged by size:
4640 599 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3072 3262 4023 5051 1540
Size: 275 186 1071 190 761 1028 2301
Fragments arranged by size:
2301 1071 1028 761 275 190 186
BsiHKAI G_wGCw'C
Cuts at: 2853 3873 3958 5029 2853
Size: 1020 85 1071 3636
Fragments arranged by size:
3636 1071 1020 85
BsmI GAATG_Cn'
Cuts at: 3323 3416 3323
Size: 93 5719
BsmBI CGTCTCn'nnnn_
Cuts at: 2269 2269
Size: 5812
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2747 2831 2853 2937 3131 3873 3958
Size: 399 957 84 22 84 194 742 85
Cuts at: 3958 5029 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 957 742 399 194 85 84
84 22
BspEI T'CCGG_A
Cuts at: 5475 5475
Size: 5812
BspHI T'CATG_A
Cuts at: 3701 5431 3701
Size: 1730 4082
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2400 2529 3167 844
Size: 1556 129 638 3489
Fragments arranged by size:
3489 1556 638 129
BsrBI CCG'CTC
Cuts at: 1394 1823 3699 1394
Size: 429 1876 3507
Fragments arranged by size:
3507 1876 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4307 4481 65
Size: 1183 3059 174 1396
Fragments arranged by size:
3059 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2147 2463 2478 4456 4643 1700
Size: 110 337 316 15 1978 187 2869
Fragments arranged by size:
2869 1978 337 316 187 110 15
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5812
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5812
BssSI C'ACGA_G
Cuts at: 3872 4884 3872
Size: 1012 4800
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2880 794
Size: 2086 3726
BstBI TT'CG_AA
Cuts at: 4616 4616
Size: 5812
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5812
BstYI r'GATC_y
Cuts at: 1972 3142 3509 3894 3911 5363 5449 5461 5807
Size: 1170 367 385 17 1452 86 12 346
Cuts at: 5807 1972
Size: 1977
Fragments arranged by size:
1977 1452 1170 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2129 2129
Size: 5812
BtgI C'CryG_G
Cuts at: 513 1065 2004 2747 3023 513
Size: 552 939 743 276 3302
Fragments arranged by size:
3302 939 743 552 276
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2125 487
Size: 683 56 899 4174
Fragments arranged by size:
4174 899 683 56
BtsI GCAGTG_nn'
Cuts at: 3328 4122 4142 3328
Size: 794 20 4998
Fragments arranged by size:
4998 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5812
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5777
DraI TTT'AAA
Cuts at: 3116 3116
Size: 5812
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4819 809
Size: 507 3503 1802
Fragments arranged by size:
3503 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3069 3259 4148
Size: 54 1425 50 275 18 1239 190 889
Cuts at: 4148 5777 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1239 889 275 190 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3069 3259 1537
Size: 275 1257 190 4090
Fragments arranged by size:
4090 1257 275 190
EarI CTCTTCn'nnn_
Cuts at: 1353 2219 3742 1353
Size: 866 1523 3423
Fragments arranged by size:
3423 1523 866
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4399 4773 4919 387
Size: 1064 138 2810 374 146 1280
Fragments arranged by size:
2810 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2128 3875 4472 5259 5527 1239
Size: 393 496 1747 597 787 268 1524
Fragments arranged by size:
1747 1524 787 597 496 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2282 2282
Size: 5812
EcoO109I rG'GnC_Cy
Cuts at: 2024 2365 2024
Size: 341 5471
EcoRI G'AATT_C
Cuts at: 3121 3499 3121
Size: 378 5434
FspI TGC'GCA
Cuts at: 4318 4318
Size: 5812
HaeII r_GCGC'y
Cuts at: 1678 2018 2413 2959 4959 1678
Size: 340 395 546 2000 2531
Fragments arranged by size:
2531 2000 546 395 340
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2026 2058 4453 4485 4527
Size: 32 135 32 454 32 2395 32 42
Cuts at: 4527 4559 1373
Size: 32 2626
Fragments arranged by size:
2626 2395 454 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2272 3156 3402 669
Size: 988 281 334 884 246 3079
Fragments arranged by size:
3079 988 884 334 281 246
HindIII A'AGCT_T
Cuts at: 748 2625 748
Size: 1877 3935
HpaI GTT'AAC
Cuts at: 3402 3402
Size: 5812
KasI G'GCGC_C
Cuts at: 2014 2014
Size: 5812
KpnI G_GTAC'C
Cuts at: 2470 3137 2470
Size: 667 5145
MfeI C'AATT_G
Cuts at: 3411 3411
Size: 5812
MluI A'CGCG_T
Cuts at: 2808 5688 2808
Size: 2880 2932
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4926 5110 1305
Size: 3621 184 2007
Fragments arranged by size:
3621 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3990
Fragments arranged by size:
3990 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2192 2819 3770 4129 4288 518
Size: 648 1026 627 951 359 159 2042
Fragments arranged by size:
2042 1026 951 648 627 359 159
MspA1I CmG'CkG
Cuts at: 1606 2183 2679 3068 3228 3905 5053 5298 1606
Size: 577 496 389 160 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 577 496 389 245 160
NaeI GCC'GGC
Cuts at: 2480 2480
Size: 5812
NarI GG'CG_CC
Cuts at: 2015 2015
Size: 5812
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4321
Fragments arranged by size:
4321 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5812
NgoMIV G'CCGG_C
Cuts at: 2478 2478
Size: 5812
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5812
NotI GC'GGCC_GC
Cuts at: 3259 3259
Size: 5812
NspI r_CATG'y
Cuts at: 1132 1229 2990 3170 4715 1132
Size: 97 1761 180 1545 2229
Fragments arranged by size:
2229 1761 1545 180 97
PasI CC'CwG_GG
Cuts at: 1348 2831 1348
Size: 1483 4329
PciI A'CATG_T
Cuts at: 4711 4711
Size: 5812
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 4910
PfoI T'CCnGG_A
Cuts at: 3103 3103
Size: 5812
PmeI GTTT'AAAC
Cuts at: 3116 3116
Size: 5812
PmlI CAC'GTG
Cuts at: 2236 2236
Size: 5812
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3881 3913 5394 5426 3881
Size: 32 1481 32 4267
Fragments arranged by size:
4267 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2024 2024
Size: 5812
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5812
PsiI TTA'TAA
Cuts at: 3382 3382
Size: 5812
PspXI vC'TCGA_Gb
Cuts at: 2090 2090
Size: 5812
PstI C_TGCA'G
Cuts at: 830 2310 2397 2642 3164 830
Size: 1480 87 245 522 3478
Fragments arranged by size:
3478 1480 522 245 87
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5812
SalI G'TCGA_C
Cuts at: 1936 3154 1936
Size: 1218 4594
SanDI GG'GwC_CC
Cuts at: 2024 2024
Size: 5812
SbfI CC_TGCA'GG
Cuts at: 2397 3164 2397
Size: 767 5045
ScaI AGT'ACT
Cuts at: 4060 5772 4060
Size: 1712 4100
SfcI C'TryA_G
Cuts at: 826 1045 2306 2393 2638 2734 3160 4295 4976
Size: 219 1261 87 245 96 426 1135 681
Cuts at: 4976 5167 826
Size: 191 1471
Fragments arranged by size:
1471 1261 1135 681 426 245 219 191
96 87
SfoI GGC'GCC
Cuts at: 2016 2016
Size: 5812
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5812
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5812
SmaI CCC'GGG
Cuts at: 1872 2546 3139 1872
Size: 674 593 4545
Fragments arranged by size:
4545 674 593
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2090 3916 4817 5079 820
Size: 197 926 147 1826 901 262 1553
Fragments arranged by size:
1826 1553 926 901 262 197 147
SnaBI TAC'GTA
Cuts at: 493 3603 493
Size: 3110 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5812
SphI G_CATG'C
Cuts at: 1132 2990 3170 1132
Size: 1858 180 3774
Fragments arranged by size:
3774 1858 180
SspI AAT'ATT
Cuts at: 5 52 3736 5
Size: 47 3684 2081
Fragments arranged by size:
3684 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5812
StyI C'CwwG_G
Cuts at: 513 1065 2004 2019 3251 513
Size: 552 939 15 1232 3074
Fragments arranged by size:
3074 1232 939 552 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2369 3839 3856 4009 4194 4639 325
Size: 1255 789 1470 17 153 185 445 1498
Fragments arranged by size:
1498 1470 1255 789 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2322 4058 5770
Size: 274 80 33 51 1443 345 1736 1712
Cuts at: 5770 96
Size: 138
Fragments arranged by size:
1736 1712 1443 345 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5772 5804 5772
Size: 32 5780
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5812
XbaI T'CTAG_A
Cuts at: 3148 3148
Size: 5812
XhoI C'TCGA_G
Cuts at: 1943 2090 1943
Size: 147 5665
XmaI C'CCGG_G
Cuts at: 1870 2544 3137 1870
Size: 674 593 4545
Fragments arranged by size:
4545 674 593
XmnI GAAnn'nnTTC
Cuts at: 3941 3941
Size: 5812
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3620 276
Size: 53 83 186 936 2086 2468
Fragments arranged by size:
2468 2086 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PstI PvuI SalI SanDI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BbvCI BfrBI BplI Bpu10I
BsiWI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI
FspAI NruI NsiI PacI PspOMI PsrI PvuII RsrII
SacI SacII SapI SexAI SfiI SrfI SwaI XcmI