Restriction map for
pFN21AE0486
(Circular) (Six-base) MAPSORT of: pFN21AE0486.seq Check: 7413 from: 1 to: 5245
pFN21AE0486 5245 bp
With 182 enzymes: SgfI *
October 20, 2009 10:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3055 278
Size: 53 83 186 936 1519 2468
Fragments arranged by size:
2468 1519 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2588 1937
Size: 651 4594
Acc65I G'GTAC_C
Cuts at: 2566 2566
Size: 5245
AclI AA'CG_TT
Cuts at: 1563 3372 3745 1563
Size: 1809 373 3063
Fragments arranged by size:
3063 1809 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2156 3308 4692 4960 1632
Size: 524 1152 1384 268 1917
Fragments arranged by size:
1917 1384 1152 524 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5048
AflIII A'CryG_T
Cuts at: 1203 4144 5121 1203
Size: 2941 977 1327
Fragments arranged by size:
2941 1327 977
AgeI A'CCGG_T
Cuts at: 4076 4076
Size: 5245
AhdI GACnn_n'nnGTC
Cuts at: 3974 3974
Size: 5245
AleI CACnn'nnGTG
Cuts at: 1166 2354 1166
Size: 1188 4057
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2478 1184
Size: 1294 3951
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2486 2518 2486
Size: 32 5213
AlwNI CAG_nnn'CTG
Cuts at: 2095 2315 2440 2988 4560 2095
Size: 220 125 548 1572 2780
Fragments arranged by size:
2780 1572 548 220 125
ApaI G_GGCC'C
Cuts at: 2528 2528
Size: 5245
ApaLI G'TGCA_C
Cuts at: 3302 4458 3302
Size: 1156 4089
ApoI r'AATT_y
Cuts at: 1493 2554 2780 2932 5041 1493
Size: 1061 226 152 2109 1697
Fragments arranged by size:
2109 1697 1061 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3799 160
Size: 872 2767 1606
Fragments arranged by size:
2767 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5245
AvaI C'yCGr_G
Cuts at: 1870 1943 2570 1870
Size: 73 627 4545
Fragments arranged by size:
4545 627 73
BamHI G'GATC_C
Cuts at: 2575 2942 2575
Size: 367 4878
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2566 4021 618
Size: 325 217 627 779 1455 1842
Fragments arranged by size:
1842 1455 779 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2528 2564 1391
Size: 1137 36 4072
Fragments arranged by size:
4072 1137 36
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4274
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3436 3470 1903
Size: 34 1499 34 3678
Fragments arranged by size:
3678 1499 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3137 4347 3137
Size: 1210 4035
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5245
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2429 3856 136
Size: 107 122 71 631 605 757 1427 1525
Fragments arranged by size:
1525 1427 757 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2457 5240 2457
Size: 2783 2462
BlpI GC'TnA_GC
Cuts at: 2662 2662
Size: 5245
Bme1580I G_kGCm'C
Cuts at: 1790 2072 2478 2528 3306 4462 1790
Size: 282 406 50 778 1156 2573
Fragments arranged by size:
2573 1156 778 406 282 50
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5245
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2976 3934 442
Size: 416 53 83 399 24 1559 958 1753
Fragments arranged by size:
1753 1559 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5245
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2545 2545
Size: 5245
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2155 2222 2423 2555 3905 1239
Size: 916 67 201 132 1350 2579
Fragments arranged by size:
2579 1350 916 201 132 67
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3370 4235 4533 3370
Size: 865 298 4082
Fragments arranged by size:
4082 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3908 882
Size: 617 2409 2219
Fragments arranged by size:
2409 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2473 3036 493
Size: 711 1269 563 2702
Fragments arranged by size:
2702 1269 711 563
BsaBI GATnn'nnATC
Cuts at: 5239 5239
Size: 5245
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3052 3434 4069 275
Size: 53 83 186 936 1519 382 635 1451
Fragments arranged by size:
1519 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3678 4076 4350 4497 4908 3678
Size: 398 274 147 411 4015
Fragments arranged by size:
4015 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2111 2141 1178
Size: 30 165 30 708 30 4282
Fragments arranged by size:
4282 708 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2264 1186
Size: 1078 4167
BseYI C'CCAG_C
Cuts at: 2315 4448 4944 2315
Size: 2133 496 2616
Fragments arranged by size:
2616 2133 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4672
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2695 3456 4484 1540
Size: 275 186 694 761 1028 2301
Fragments arranged by size:
2301 1028 761 694 275 186
BsiHKAI G_wGCw'C
Cuts at: 3306 3391 4462 3306
Size: 85 1071 4089
Fragments arranged by size:
4089 1071 85
BsmI GAATG_Cn'
Cuts at: 2337 2756 2849 2337
Size: 419 93 4733
Fragments arranged by size:
4733 419 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2072 2478 2528 2564 3306 3391 4462
Size: 399 282 406 50 36 742 85 1071
Cuts at: 4462 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 406 399 282 85 50
36
BspEI T'CCGG_A
Cuts at: 4908 4908
Size: 5245
BspHI T'CATG_A
Cuts at: 3134 4864 3134
Size: 1730 3515
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2505 2600 844
Size: 1661 95 3489
Fragments arranged by size:
3489 1661 95
BsrBI CCG'CTC
Cuts at: 1394 1823 3132 1394
Size: 429 1309 3507
Fragments arranged by size:
3507 1309 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3740 3914 65
Size: 1183 2492 174 1396
Fragments arranged by size:
2492 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2343 3889 4076 1700
Size: 110 533 1546 187 2869
Fragments arranged by size:
2869 1546 533 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5245
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5245
BssSI C'ACGA_G
Cuts at: 3305 4317 3305
Size: 1012 4233
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5245
BstBI TT'CG_AA
Cuts at: 4049 4049
Size: 5245
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5245
BstXI CCAn_nnnn'nTGG
Cuts at: 2062 2062
Size: 5245
BstYI r'GATC_y
Cuts at: 1972 2457 2575 2942 3327 3344 4796 4882 4894
Size: 485 118 367 385 17 1452 86 12
Cuts at: 4894 5240 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 485 385 367 346 118 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2352 2427 513
Size: 552 1287 75 3331
Fragments arranged by size:
3331 1287 552 75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4506
Fragments arranged by size:
4506 683 56
BtsI GCAGTG_nn'
Cuts at: 2329 2761 3555 3575 2329
Size: 432 794 20 3999
Fragments arranged by size:
3999 794 432 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5245
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5210
DraI TTT'AAA
Cuts at: 2549 2549
Size: 5245
DraIII CAC_nnn'GTG
Cuts at: 2353 2353
Size: 5245
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4252 809
Size: 507 2936 1802
Fragments arranged by size:
2936 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2049 2467 2692
Size: 54 1425 50 275 18 219 418 225
Cuts at: 2692 3581 5210 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 418 275 225 219 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2692 1537
Size: 275 880 4090
Fragments arranged by size:
4090 880 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2088 3175 1353
Size: 735 1087 3423
Fragments arranged by size:
3423 1087 735
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2114 3832 4206 4352 387
Size: 1064 138 525 1718 374 146 1280
Fragments arranged by size:
1718 1280 1064 525 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2155 2156 2222 2423 2555 3308 3905
Size: 393 523 1 66 201 132 753 597
Cuts at: 3905 4692 4960 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 753 597 523 393 268 201
132 66 1
EcoO109I rG'GnC_Cy
Cuts at: 2019 2524 2525 2539 2019
Size: 505 1 14 4725
Fragments arranged by size:
4725 505 14 1
EcoRI G'AATT_C
Cuts at: 2554 2932 2554
Size: 378 4867
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2161 2161
Size: 5245
FspI TGC'GCA
Cuts at: 3751 3751
Size: 5245
HaeII r_GCGC'y
Cuts at: 1678 2440 4392 1678
Size: 762 1952 2531
Fragments arranged by size:
2531 1952 762
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2111 2143 3886 3918 3960
Size: 32 135 32 539 32 1743 32 42
Cuts at: 3960 3992 1373
Size: 32 2626
Fragments arranged by size:
2626 1743 539 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2589 2835 669
Size: 988 281 651 246 3079
Fragments arranged by size:
3079 988 651 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5245
HpaI GTT'AAC
Cuts at: 2835 2835
Size: 5245
KpnI G_GTAC'C
Cuts at: 2570 2570
Size: 5245
MfeI C'AATT_G
Cuts at: 2844 2844
Size: 5245
MluI A'CGCG_T
Cuts at: 5121 5121
Size: 5245
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4359 4543 1305
Size: 3054 184 2007
Fragments arranged by size:
3054 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2469 10
Size: 54 1425 343 637 2786
Fragments arranged by size:
2786 1425 637 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2268 2354 3203 3562 3721 518
Size: 648 843 259 86 849 359 159 2042
Fragments arranged by size:
2042 849 843 648 359 259 159 86
MspA1I CmG'CkG
Cuts at: 1606 2095 2661 3338 4486 4731 1606
Size: 489 566 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 566 489 245
NcoI C'CATG_G
Cuts at: 513 1065 2427 513
Size: 552 1362 3331
Fragments arranged by size:
3331 1362 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5245
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5245
NotI GC'GGCC_GC
Cuts at: 2692 2692
Size: 5245
NspI r_CATG'y
Cuts at: 1132 1229 2603 4148 1132
Size: 97 1374 1545 2229
Fragments arranged by size:
2229 1545 1374 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5245
PciI A'CATG_T
Cuts at: 4144 4144
Size: 5245
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5245
PmeI GTTT'AAAC
Cuts at: 2549 2549
Size: 5245
PmlI CAC'GTG
Cuts at: 2473 2473
Size: 5245
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3314 3346 4827 4859 3314
Size: 32 1481 32 3700
Fragments arranged by size:
3700 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2539 2539
Size: 5245
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5245
PsiI TTA'TAA
Cuts at: 2815 2815
Size: 5245
PspOMI G'GGCC_C
Cuts at: 2524 2524
Size: 5245
PstI C_TGCA'G
Cuts at: 830 2091 2336 2457 2597 830
Size: 1261 245 121 140 3478
Fragments arranged by size:
3478 1261 245 140 121
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5245
PvuII CAG'CTG
Cuts at: 2095 2095
Size: 5245
SalI G'TCGA_C
Cuts at: 1936 2587 1936
Size: 651 4594
SapI GCTCTTCn'nnn_
Cuts at: 2088 2088
Size: 5245
SbfI CC_TGCA'GG
Cuts at: 2597 2597
Size: 5245
ScaI AGT'ACT
Cuts at: 3493 5205 3493
Size: 1712 3533
SexAI A'CCwGG_T
Cuts at: 2541 2541
Size: 5245
SfcI C'TryA_G
Cuts at: 826 1045 2087 2332 2453 2593 3728 4409 4600
Size: 219 1042 245 121 140 1135 681 191
Cuts at: 4600 826
Size: 1471
Fragments arranged by size:
1471 1135 1042 681 245 219 191 140
121
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5245
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5245
SmaI CCC'GGG
Cuts at: 1872 2572 1872
Size: 700 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3349 4250 4512 820
Size: 197 926 1406 901 262 1553
Fragments arranged by size:
1553 1406 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3036 493
Size: 2543 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5245
SphI G_CATG'C
Cuts at: 1132 2603 1132
Size: 1471 3774
SspI AAT'ATT
Cuts at: 5 52 3169 5
Size: 47 3117 2081
Fragments arranged by size:
3117 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5245
StyI C'CwwG_G
Cuts at: 513 1065 2427 2684 513
Size: 552 1362 257 3074
Fragments arranged by size:
3074 1362 552 257
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3272 3289 3442 3627 4072 325
Size: 1255 1692 17 153 185 445 1498
Fragments arranged by size:
1692 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3491 5203 96
Size: 274 80 33 51 1443 1514 1712 138
Fragments arranged by size:
1712 1514 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5205 5237 5205
Size: 32 5213
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5245
XbaI T'CTAG_A
Cuts at: 2581 2581
Size: 5245
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2059 2062 2281 2059
Size: 3 219 5023
Fragments arranged by size:
5023 219 3
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5245
XmaI C'CCGG_G
Cuts at: 1870 2570 1870
Size: 700 4545
XmnI GAAnn'nnTTC
Cuts at: 3374 3374
Size: 5245
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3053 276
Size: 53 83 186 936 1519 2468
Fragments arranged by size:
2468 1519 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PspXI PsrI RsrII SacI SacII SanDI
SfiI SfoI SrfI SwaI