Restriction map for
pFN21AE0475
(Circular) (Six-base) MAPSORT of: pFN21AE0475.seq Check: 8285 from: 1 to: 5461
pFN21AE0475 5461 bp
With 182 enzymes: SgfI *
October 20, 2009 10:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3271 278
Size: 53 83 186 936 1735 2468
Fragments arranged by size:
2468 1735 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2562 2804 1937
Size: 625 242 4594
Fragments arranged by size:
4594 625 242
Acc65I G'GTAC_C
Cuts at: 2782 2782
Size: 5461
AclI AA'CG_TT
Cuts at: 1563 3588 3961 1563
Size: 2025 373 3063
Fragments arranged by size:
3063 2025 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3524 4908 5176 1632
Size: 1892 1384 268 1917
Fragments arranged by size:
1917 1892 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5264
AflIII A'CryG_T
Cuts at: 1203 4360 5337 1203
Size: 3157 977 1327
Fragments arranged by size:
3157 1327 977
AgeI A'CCGG_T
Cuts at: 4292 4292
Size: 5461
AhdI GACnn_n'nnGTC
Cuts at: 2558 4190 2558
Size: 1632 3829
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5461
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2170 1184
Size: 986 4475
AlwNI CAG_nnn'CTG
Cuts at: 3204 4776 3204
Size: 1572 3889
ApaLI G'TGCA_C
Cuts at: 3518 4674 3518
Size: 1156 4305
ApoI r'AATT_y
Cuts at: 1493 2281 2718 2770 2996 3148 5257 1493
Size: 788 437 52 226 152 2109 1697
Fragments arranged by size:
2109 1697 788 437 226 152 52
AseI AT'TA_AT
Cuts at: 160 1032 4015 160
Size: 872 2983 1606
Fragments arranged by size:
2983 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5461
AvaI C'yCGr_G
Cuts at: 1870 1943 2097 2786 1870
Size: 73 154 689 4545
Fragments arranged by size:
4545 689 154 73
BamHI G'GATC_C
Cuts at: 2791 3158 2791
Size: 367 5094
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2025 2782 4237 618
Size: 325 217 627 238 757 1455 1842
Fragments arranged by size:
1842 1455 757 627 325 238 217
BanII G_rGCy'C
Cuts at: 1391 2058 2780 1391
Size: 667 722 4072
Fragments arranged by size:
4072 722 667
BbeI G_GCGC'C
Cuts at: 2029 2029
Size: 5461
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2369 928
Size: 971 470 4020
Fragments arranged by size:
4020 971 470
BbvCI CC'TCA_GC
Cuts at: 2066 2066
Size: 5461
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3652 3686 1903
Size: 34 1715 34 3678
Fragments arranged by size:
3678 1715 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3353 4563 3353
Size: 1210 4251
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5461
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4072 136
Size: 107 122 71 631 605 2400 1525
Fragments arranged by size:
2400 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2683 5456 2683
Size: 2773 2688
BlpI GC'TnA_GC
Cuts at: 2878 2878
Size: 5461
Bme1580I G_kGCm'C
Cuts at: 1790 2189 3522 4678 1790
Size: 399 1333 1156 2573
Fragments arranged by size:
2573 1333 1156 399
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5461
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2282 3192 4150
Size: 416 53 83 399 24 865 910 958
Cuts at: 4150 442
Size: 1753
Fragments arranged by size:
1753 958 910 865 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5461
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2595 4121 1239
Size: 1356 1526 2579
Fragments arranged by size:
2579 1526 1356
Bpu10I CC'TnA_GC
Cuts at: 2066 2066
Size: 5461
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3586 4451 4749 3586
Size: 865 298 4298
Fragments arranged by size:
4298 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2129 2295 4124 882
Size: 617 630 166 1829 2219
Fragments arranged by size:
2219 1829 630 617 166
BsaAI yAC'GTr
Cuts at: 493 1204 3252 493
Size: 711 2048 2702
Fragments arranged by size:
2702 2048 711
BsaBI GATnn'nnATC
Cuts at: 2682 5455 2682
Size: 2773 2688
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2026 3268 3650 4285
Size: 53 83 186 936 493 1242 382 635
Cuts at: 4285 275
Size: 1451
Fragments arranged by size:
1451 1242 936 635 493 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2498 3894 4292 4566 4713 5124 2498
Size: 1396 398 274 147 411 2835
Fragments arranged by size:
2835 1396 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5236
Fragments arranged by size:
5236 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2093 2666 1186
Size: 907 573 3981
Fragments arranged by size:
3981 907 573
BseYI C'CCAG_C
Cuts at: 2626 4664 5160 2626
Size: 2038 496 2927
Fragments arranged by size:
2927 2038 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2166 2418 1163
Size: 573 430 252 4206
Fragments arranged by size:
4206 573 430 252
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2911 3672 4700 1540
Size: 275 186 910 761 1028 2301
Fragments arranged by size:
2301 1028 910 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2743 3522 3607 4678 2743
Size: 779 85 1071 3526
Fragments arranged by size:
3526 1071 779 85
BsmI GAATG_Cn'
Cuts at: 2972 3065 2972
Size: 93 5368
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2058 2189 2743 2780 3522 3607 4678
Size: 399 268 131 554 37 742 85 1071
Cuts at: 4678 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 554 399 268 131 85
37
BspEI T'CCGG_A
Cuts at: 5124 5124
Size: 5461
BspHI T'CATG_A
Cuts at: 3350 5080 3350
Size: 1730 3731
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2548 2816 844
Size: 1704 268 3489
Fragments arranged by size:
3489 1704 268
BsrBI CCG'CTC
Cuts at: 1394 1823 3348 1394
Size: 429 1525 3507
Fragments arranged by size:
3507 1525 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3956 4130 65
Size: 1183 2708 174 1396
Fragments arranged by size:
2708 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4105 4292 1700
Size: 110 2295 187 2869
Fragments arranged by size:
2869 2295 187 110
BsrGI T'GTAC_A
Cuts at: 96 2311 96
Size: 2215 3246
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5461
BssSI C'ACGA_G
Cuts at: 3521 4533 3521
Size: 1012 4449
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5461
BstBI TT'CG_AA
Cuts at: 4265 4265
Size: 5461
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5461
BstYI r'GATC_y
Cuts at: 1972 2398 2683 2791 3158 3543 3560 5012 5098
Size: 426 285 108 367 385 17 1452 86
Cuts at: 5098 5110 5456 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 426 385 367 346 285 108
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2130 2594 513
Size: 552 939 126 464 3380
Fragments arranged by size:
3380 939 552 464 126
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4722
Fragments arranged by size:
4722 683 56
BtsI GCAGTG_nn'
Cuts at: 2149 2340 2435 2977 3771 3791 2149
Size: 191 95 542 794 20 3819
Fragments arranged by size:
3819 794 542 191 95 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5461
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5426
DraI TTT'AAA
Cuts at: 2765 2765
Size: 5461
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4468 809
Size: 507 3152 1802
Fragments arranged by size:
3152 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2908 3797 5426
Size: 54 1425 50 275 18 1078 889 1629
Cuts at: 5426 8
Size: 43
Fragments arranged by size:
1629 1425 1078 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2908 1537
Size: 275 1096 4090
Fragments arranged by size:
4090 1096 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2594 3391 1353
Size: 1241 797 3423
Fragments arranged by size:
3423 1241 797
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4048 4422 4568 387
Size: 1064 138 2459 374 146 1280
Fragments arranged by size:
2459 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2595 3524 4121 4908 5176 1239
Size: 393 963 929 597 787 268 1524
Fragments arranged by size:
1524 963 929 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2194 2194
Size: 5461
EcoRI G'AATT_C
Cuts at: 2281 2770 3148 2281
Size: 489 378 4594
Fragments arranged by size:
4594 489 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2206 2417 2206
Size: 211 5250
FspI TGC'GCA
Cuts at: 3967 3967
Size: 5461
HaeII r_GCGC'y
Cuts at: 1678 2029 4608 1678
Size: 351 2579 2531
Fragments arranged by size:
2579 2531 351
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2544 2576 4102 4134 4176
Size: 32 135 32 972 32 1526 32 42
Cuts at: 4176 4208 1373
Size: 32 2626
Fragments arranged by size:
2626 1526 972 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2563 2805 3051 669
Size: 988 281 625 242 246 3079
Fragments arranged by size:
3079 988 625 281 246 242
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5461
HpaI GTT'AAC
Cuts at: 3051 3051
Size: 5461
KasI G'GCGC_C
Cuts at: 2025 2025
Size: 5461
KpnI G_GTAC'C
Cuts at: 2786 2786
Size: 5461
MfeI C'AATT_G
Cuts at: 3060 3060
Size: 5461
MluI A'CGCG_T
Cuts at: 5337 5337
Size: 5461
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1992 4575 4759 1305
Size: 687 2583 184 2007
Fragments arranged by size:
2583 2007 687 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3639
Fragments arranged by size:
3639 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3419 3778 3937 518
Size: 648 2253 359 159 2042
Fragments arranged by size:
2253 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2877 3554 4702 4947 1606
Size: 1271 677 1148 245 2120
Fragments arranged by size:
2120 1271 1148 677 245
NarI GG'CG_CC
Cuts at: 2026 2026
Size: 5461
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3970
Fragments arranged by size:
3970 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5461
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5461
NotI GC'GGCC_GC
Cuts at: 2908 2908
Size: 5461
NspI r_CATG'y
Cuts at: 1132 1229 2065 2451 2819 4364 1132
Size: 97 836 386 368 1545 2229
Fragments arranged by size:
2229 1545 836 386 368 97
PacI TTA_AT'TAA
Cuts at: 2211 2211
Size: 5461
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5461
PciI A'CATG_T
Cuts at: 4360 4360
Size: 5461
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5461
PfoI T'CCnGG_A
Cuts at: 2174 2174
Size: 5461
PmeI GTTT'AAAC
Cuts at: 2765 2765
Size: 5461
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3530 3562 5043 5075 3530
Size: 32 1481 32 3916
Fragments arranged by size:
3916 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5461
PsiI TTA'TAA
Cuts at: 3031 3031
Size: 5461
PstI C_TGCA'G
Cuts at: 830 2813 830
Size: 1983 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5461
SalI G'TCGA_C
Cuts at: 1936 2561 2803 1936
Size: 625 242 4594
Fragments arranged by size:
4594 625 242
SbfI CC_TGCA'GG
Cuts at: 2813 2813
Size: 5461
ScaI AGT'ACT
Cuts at: 2084 3709 5421 2084
Size: 1625 1712 2124
Fragments arranged by size:
2124 1712 1625
SfcI C'TryA_G
Cuts at: 826 1045 2751 2809 3944 4625 4816 826
Size: 219 1706 58 1135 681 191 1471
Fragments arranged by size:
1706 1471 1135 681 219 191 58
SfoI GGC'GCC
Cuts at: 2027 2027
Size: 5461
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5461
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5461
SmaI CCC'GGG
Cuts at: 1872 2099 2788 1872
Size: 227 689 4545
Fragments arranged by size:
4545 689 227
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3565 4466 4728 820
Size: 197 926 1622 901 262 1553
Fragments arranged by size:
1622 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3252 493
Size: 2759 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5461
SphI G_CATG'C
Cuts at: 1132 2451 2819 1132
Size: 1319 368 3774
Fragments arranged by size:
3774 1319 368
SspI AAT'ATT
Cuts at: 5 52 2290 3385 5
Size: 47 2238 1095 2081
Fragments arranged by size:
2238 2081 1095 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5461
StyI C'CwwG_G
Cuts at: 513 1065 2004 2190 2900 513
Size: 552 939 186 710 3074
Fragments arranged by size:
3074 939 710 552 186
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3488 3505 3658 3843 4288 325
Size: 1255 1908 17 153 185 445 1498
Fragments arranged by size:
1908 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2082 2311 3707
Size: 274 80 33 51 1443 105 229 1396
Cuts at: 3707 5419 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1396 274 229 138 105 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5421 5453 5421
Size: 32 5429
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5461
XbaI T'CTAG_A
Cuts at: 2797 2797
Size: 5461
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5461
XmaI C'CCGG_G
Cuts at: 1870 2097 2786 1870
Size: 227 689 4545
Fragments arranged by size:
4545 689 227
XmnI GAAnn'nnTTC
Cuts at: 3590 3590
Size: 5461
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3269 276
Size: 53 83 186 936 1735 2468
Fragments arranged by size:
2468 1735 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PacI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoO109I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI