Restriction map for
pFN21AE0474
(Circular) (Six-base) MAPSORT of: pFN21AE0474.seq Check: 9307 from: 1 to: 5206
pFN21AE0474 5206 bp
With 182 enzymes: SgfI *
October 20, 2009 10:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3016 278
Size: 53 83 186 936 1480 2468
Fragments arranged by size:
2468 1480 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2549 1937
Size: 612 4594
Acc65I G'GTAC_C
Cuts at: 2527 2527
Size: 5206
AclI AA'CG_TT
Cuts at: 1563 3333 3706 1563
Size: 1770 373 3063
Fragments arranged by size:
3063 1770 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2458 3269 4653 4921 1632
Size: 826 811 1384 268 1917
Fragments arranged by size:
1917 1384 826 811 268
AflII C'TTAA_G
Cuts at: 820 1017 2105 820
Size: 197 1088 3921
Fragments arranged by size:
3921 1088 197
AflIII A'CryG_T
Cuts at: 1203 4105 5082 1203
Size: 2902 977 1327
Fragments arranged by size:
2902 1327 977
AgeI A'CCGG_T
Cuts at: 4037 4037
Size: 5206
AhdI GACnn_n'nnGTC
Cuts at: 3935 3935
Size: 5206
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5206
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2325 1184
Size: 1141 4065
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2172 2204 2172
Size: 32 5174
AlwNI CAG_nnn'CTG
Cuts at: 2021 2949 4521 2021
Size: 928 1572 2706
Fragments arranged by size:
2706 1572 928
ApaLI G'TGCA_C
Cuts at: 3263 4419 3263
Size: 1156 4050
ApoI r'AATT_y
Cuts at: 1493 2267 2515 2741 2893 5002 1493
Size: 774 248 226 152 2109 1697
Fragments arranged by size:
2109 1697 774 248 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3760 160
Size: 872 2728 1606
Fragments arranged by size:
2728 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5206
AvaI C'yCGr_G
Cuts at: 1870 1943 2531 1870
Size: 73 588 4545
Fragments arranged by size:
4545 588 73
BamHI G'GATC_C
Cuts at: 2536 2903 2536
Size: 367 4839
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2326 2527 3982 618
Size: 325 217 627 539 201 1455 1842
Fragments arranged by size:
1842 1455 627 539 325 217 201
BanII G_rGCy'C
Cuts at: 1391 2218 2525 1391
Size: 827 307 4072
Fragments arranged by size:
4072 827 307
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4235
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3397 3431 1903
Size: 34 1460 34 3678
Fragments arranged by size:
3678 1460 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3098 4308 3098
Size: 1210 3996
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5206
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3817 136
Size: 107 122 71 631 605 2145 1525
Fragments arranged by size:
2145 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2028 5201 2028
Size: 3173 2033
BlpI GC'TnA_GC
Cuts at: 2623 2623
Size: 5206
Bme1580I G_kGCm'C
Cuts at: 1790 2329 3267 4423 1790
Size: 539 938 1156 2573
Fragments arranged by size:
2573 1156 938 539
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5206
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2937 3895 442
Size: 416 53 83 399 24 1520 958 1753
Fragments arranged by size:
1753 1520 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5206
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2148 3866 1239
Size: 909 1718 2579
Fragments arranged by size:
2579 1718 909
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3331 4196 4494 3331
Size: 865 298 4043
Fragments arranged by size:
4043 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3869 882
Size: 617 2370 2219
Fragments arranged by size:
2370 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2997 493
Size: 711 1793 2702
Fragments arranged by size:
2702 1793 711
BsaBI GATnn'nnATC
Cuts at: 5200 5200
Size: 5206
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3013 3395 4030 275
Size: 53 83 186 936 1480 382 635 1451
Fragments arranged by size:
1480 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3639 4037 4311 4458 4869 3639
Size: 398 274 147 411 3976
Fragments arranged by size:
3976 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4981
Fragments arranged by size:
4981 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5206
BseYI C'CCAG_C
Cuts at: 4409 4905 4409
Size: 496 4710
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4633
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2656 3417 4445 1540
Size: 275 186 655 761 1028 2301
Fragments arranged by size:
2301 1028 761 655 275 186
BsiHKAI G_wGCw'C
Cuts at: 2218 3267 3352 4423 2218
Size: 1049 85 1071 3001
Fragments arranged by size:
3001 1071 1049 85
BsmI GAATG_Cn'
Cuts at: 2717 2810 2717
Size: 93 5113
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2218 2329 2525 3267 3352 4423 1391
Size: 399 428 111 196 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 428 399 196 111 85
BspEI T'CCGG_A
Cuts at: 4869 4869
Size: 5206
BspHI T'CATG_A
Cuts at: 3095 4825 3095
Size: 1730 3476
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2561 844
Size: 1717 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3093 1394
Size: 429 1270 3507
Fragments arranged by size:
3507 1270 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2491 3701 3875 65
Size: 1183 1243 1210 174 1396
Fragments arranged by size:
1396 1243 1210 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3850 4037 1700
Size: 110 2040 187 2869
Fragments arranged by size:
2869 2040 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5206
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5206
BssSI C'ACGA_G
Cuts at: 3266 4278 3266
Size: 1012 4194
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5206
BstBI TT'CG_AA
Cuts at: 4010 4010
Size: 5206
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5206
BstYI r'GATC_y
Cuts at: 1972 2028 2536 2903 3288 3305 4757 4843 4855
Size: 56 508 367 385 17 1452 86 12
Cuts at: 4855 5201 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 508 385 367 346 86 56
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4654
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4467
Fragments arranged by size:
4467 683 56
BtsI GCAGTG_nn'
Cuts at: 2304 2722 3516 3536 2304
Size: 418 794 20 3974
Fragments arranged by size:
3974 794 418 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5206
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5171
DraI TTT'AAA
Cuts at: 2510 2510
Size: 5206
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4213 809
Size: 507 2897 1802
Fragments arranged by size:
2897 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2198 2653 3542
Size: 54 1425 50 275 18 368 455 889
Cuts at: 3542 5171 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 455 368 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2653 1537
Size: 275 841 4090
Fragments arranged by size:
4090 841 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2271 3136 1353
Size: 918 865 3423
Fragments arranged by size:
3423 918 865
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3793 4167 4313 387
Size: 1064 138 2204 374 146 1280
Fragments arranged by size:
2204 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2216 2216
Size: 5206
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2148 2458 3269 3866 4653 4921 1239
Size: 393 516 310 811 597 787 268 1524
Fragments arranged by size:
1524 811 787 597 516 393 310 268
EcoRI G'AATT_C
Cuts at: 2515 2893 2515
Size: 378 4828
FspI TGC'GCA
Cuts at: 3712 3712
Size: 5206
HaeII r_GCGC'y
Cuts at: 1678 4353 1678
Size: 2675 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3847 3879 3921 3953 1373
Size: 32 135 32 2275 32 42 32 2626
Fragments arranged by size:
2626 2275 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2550 2796 669
Size: 988 281 612 246 3079
Fragments arranged by size:
3079 988 612 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5206
HpaI GTT'AAC
Cuts at: 2796 2796
Size: 5206
KpnI G_GTAC'C
Cuts at: 2531 2531
Size: 5206
MfeI C'AATT_G
Cuts at: 2805 2805
Size: 5206
MluI A'CGCG_T
Cuts at: 5082 5082
Size: 5206
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4320 4504 1305
Size: 3015 184 2007
Fragments arranged by size:
3015 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2200 10
Size: 54 1425 343 368 3016
Fragments arranged by size:
3016 1425 368 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2496 3164 3523 3682 518
Size: 648 1330 668 359 159 2042
Fragments arranged by size:
2042 1330 668 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2431 2622 3299 4447 4692 1606
Size: 825 191 677 1148 245 2120
Fragments arranged by size:
2120 1148 825 677 245 191
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4654
NdeI CA'TA_TG
Cuts at: 387 2152 387
Size: 1765 3441
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5206
NotI GC'GGCC_GC
Cuts at: 2653 2653
Size: 5206
NspI r_CATG'y
Cuts at: 1132 1229 2047 2564 4109 1132
Size: 97 818 517 1545 2229
Fragments arranged by size:
2229 1545 818 517 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5206
PciI A'CATG_T
Cuts at: 4105 4105
Size: 5206
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5206
PmeI GTTT'AAAC
Cuts at: 2510 2510
Size: 5206
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3275 3307 4788 4820 3275
Size: 32 1481 32 3661
Fragments arranged by size:
3661 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5206
PsiI TTA'TAA
Cuts at: 2393 2776 2393
Size: 383 4823
PstI C_TGCA'G
Cuts at: 830 2558 830
Size: 1728 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5206
PvuII CAG'CTG
Cuts at: 2431 2431
Size: 5206
SacI G_AGCT'C
Cuts at: 2218 2218
Size: 5206
SalI G'TCGA_C
Cuts at: 1936 2548 1936
Size: 612 4594
SapI GCTCTTCn'nnn_
Cuts at: 2271 2271
Size: 5206
SbfI CC_TGCA'GG
Cuts at: 2558 2558
Size: 5206
ScaI AGT'ACT
Cuts at: 3454 5166 3454
Size: 1712 3494
SfcI C'TryA_G
Cuts at: 826 1045 2554 3689 4370 4561 826
Size: 219 1509 1135 681 191 1471
Fragments arranged by size:
1509 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5206
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5206
SmaI CCC'GGG
Cuts at: 1872 2533 1872
Size: 661 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2105 3310 4211 4473 820
Size: 197 926 162 1205 901 262 1553
Fragments arranged by size:
1553 1205 926 901 262 197 162
SnaBI TAC'GTA
Cuts at: 493 2997 493
Size: 2504 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5206
SphI G_CATG'C
Cuts at: 1132 2564 1132
Size: 1432 3774
SspI AAT'ATT
Cuts at: 5 52 3130 5
Size: 47 3078 2081
Fragments arranged by size:
3078 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5206
StyI C'CwwG_G
Cuts at: 513 1065 2645 513
Size: 552 1580 3074
Fragments arranged by size:
3074 1580 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3233 3250 3403 3588 4033 325
Size: 1255 1653 17 153 185 445 1498
Fragments arranged by size:
1653 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3452 5164 96
Size: 274 80 33 51 1443 1475 1712 138
Fragments arranged by size:
1712 1475 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5166 5198 5166
Size: 32 5174
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5206
XbaI T'CTAG_A
Cuts at: 2191 2542 2191
Size: 351 4855
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5206
XmaI C'CCGG_G
Cuts at: 1870 2531 1870
Size: 661 4545
XmnI GAAnn'nnTTC
Cuts at: 3335 3335
Size: 5206
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3014 276
Size: 53 83 186 936 1480 2468
Fragments arranged by size:
2468 1480 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI XcmI