Restriction map for
pFN21AE0471
(Circular) (Six-base) MAPSORT of: pFN21AE0471.seq Check: 9352 from: 1 to: 6022
pFN21AE0471 6022 bp
With 182 enzymes: SgfI *
October 20, 2009 10:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3832 278
Size: 53 83 186 936 2296 2468
Fragments arranged by size:
2468 2296 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3365 1937
Size: 1428 4594
Acc65I G'GTAC_C
Cuts at: 3343 3343
Size: 6022
AclI AA'CG_TT
Cuts at: 1563 4149 4522 1563
Size: 2586 373 3063
Fragments arranged by size:
3063 2586 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4085 5469 5737 1632
Size: 2453 1384 268 1917
Fragments arranged by size:
2453 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5825
AflIII A'CryG_T
Cuts at: 1203 4921 5898 1203
Size: 3718 977 1327
Fragments arranged by size:
3718 1327 977
AgeI A'CCGG_T
Cuts at: 4853 4853
Size: 6022
AhdI GACnn_n'nnGTC
Cuts at: 4751 4751
Size: 6022
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6022
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6022
AlwNI CAG_nnn'CTG
Cuts at: 3765 5337 3765
Size: 1572 4450
ApaI G_GGCC'C
Cuts at: 2159 2533 3116 2159
Size: 374 583 5065
Fragments arranged by size:
5065 583 374
ApaLI G'TGCA_C
Cuts at: 4079 5235 4079
Size: 1156 4866
ApoI r'AATT_y
Cuts at: 1493 2258 2339 2546 3331 3557 3709 5818 1493
Size: 765 81 207 785 226 152 2109 1697
Fragments arranged by size:
2109 1697 785 765 226 207 152 81
AseI AT'TA_AT
Cuts at: 160 1032 4576 160
Size: 872 3544 1606
Fragments arranged by size:
3544 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6022
AvaI C'yCGr_G
Cuts at: 1870 1943 2970 3219 3347 1870
Size: 73 1027 249 128 4545
Fragments arranged by size:
4545 1027 249 128 73
BamHI G'GATC_C
Cuts at: 2821 3352 3719 2821
Size: 531 367 5124
Fragments arranged by size:
5124 531 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2996 3343 4798 618
Size: 325 217 627 1209 347 1455 1842
Fragments arranged by size:
1842 1455 1209 627 347 325 217
BanII G_rGCy'C
Cuts at: 1391 2159 2533 2990 3116 3341 1391
Size: 768 374 457 126 225 4072
Fragments arranged by size:
4072 768 457 374 225 126
BbeI G_GCGC'C
Cuts at: 3000 3000
Size: 6022
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2676 928
Size: 971 777 4274
Fragments arranged by size:
4274 971 777
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4213 4247 1903
Size: 34 2276 34 3678
Fragments arranged by size:
3678 2276 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3914 5124 3914
Size: 1210 4812
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6022
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2537 2636 2994
Size: 107 122 71 631 605 865 99 358
Cuts at: 2994 4633 136
Size: 1639 1525
Fragments arranged by size:
1639 1525 865 631 605 358 122 107
99 71
BglII A'GATC_T
Cuts at: 6017 6017
Size: 6022
BlpI GC'TnA_GC
Cuts at: 3439 3439
Size: 6022
Bme1580I G_kGCm'C
Cuts at: 1790 2159 2533 2772 3116 4083 5239 1790
Size: 369 374 239 344 967 1156 2573
Fragments arranged by size:
2573 1156 967 374 369 344 239
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6022
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2162 2449 3753
Size: 416 53 83 399 24 745 287 1304
Cuts at: 3753 4711 442
Size: 958 1753
Fragments arranged by size:
1753 1304 958 745 416 399 287 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6022
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2054 2302 2372 2710 2730 2737 4682 1239
Size: 815 248 70 338 20 7 1945 2579
Fragments arranged by size:
2579 1945 815 338 248 70 20 7
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3172 4147 5012 5310 3172
Size: 975 865 298 3884
Fragments arranged by size:
3884 975 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2120 2292 2636 4685 882
Size: 617 621 172 344 2049 2219
Fragments arranged by size:
2219 2049 621 617 344 172
BsaAI yAC'GTr
Cuts at: 493 1204 3813 493
Size: 711 2609 2702
Fragments arranged by size:
2702 2609 711
BsaBI GATnn'nnATC
Cuts at: 6016 6016
Size: 6022
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2997 3829 4211 4846
Size: 53 83 186 936 1464 832 382 635
Cuts at: 4846 275
Size: 1451
Fragments arranged by size:
1464 1451 936 832 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4455 4853 5127 5274 5685 4455
Size: 398 274 147 411 4792
Fragments arranged by size:
4792 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2027 2057 2460 2490 1178
Size: 30 165 30 624 30 403 30 4710
Fragments arranged by size:
4710 624 403 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2138 2567 2915 3279 1186
Size: 952 429 348 364 3929
Fragments arranged by size:
3929 952 429 364 348
BseYI C'CCAG_C
Cuts at: 5225 5721 5225
Size: 496 5526
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2065 2498 1163
Size: 573 329 433 4687
Fragments arranged by size:
4687 573 433 329
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3472 4233 5261 1540
Size: 275 186 1471 761 1028 2301
Fragments arranged by size:
2301 1471 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2246 4083 4168 5239 2246
Size: 1837 85 1071 3029
Fragments arranged by size:
3029 1837 1071 85
BsmI GAATG_Cn'
Cuts at: 2665 3533 3626 2665
Size: 868 93 5061
Fragments arranged by size:
5061 868 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2159 2246 2533 2772 2990 3116 3341
Size: 399 369 87 287 239 218 126 225
Cuts at: 3341 4083 4168 5239 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 369 287 239 225
218 126 87 85
BspEI T'CCGG_A
Cuts at: 5685 5685
Size: 6022
BspHI T'CATG_A
Cuts at: 3911 5641 3911
Size: 1730 4292
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3377 844
Size: 2533 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3080 3909 1394
Size: 429 1257 829 3507
Fragments arranged by size:
3507 1257 829 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4517 4691 65
Size: 1183 3269 174 1396
Fragments arranged by size:
3269 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3160 4666 4853 1700
Size: 110 1350 1506 187 2869
Fragments arranged by size:
2869 1506 1350 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6022
BssHII G'CGCG_C
Cuts at: 1925 2166 1925
Size: 241 5781
BssSI C'ACGA_G
Cuts at: 4082 5094 4082
Size: 1012 5010
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6022
BstBI TT'CG_AA
Cuts at: 4826 4826
Size: 6022
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6022
BstXI CCAn_nnnn'nTGG
Cuts at: 2017 2439 2695 3020 2017
Size: 422 256 325 5019
Fragments arranged by size:
5019 422 325 256
BstYI r'GATC_y
Cuts at: 1972 2505 2821 3352 3719 4104 4121 5573 5659
Size: 533 316 531 367 385 17 1452 86
Cuts at: 5659 5671 6017 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 533 531 385 367 346 316
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2826 2961 2826
Size: 135 5887
BtgI C'CryG_G
Cuts at: 513 1065 2016 2664 2922 3051 513
Size: 552 951 648 258 129 3484
Fragments arranged by size:
3484 951 648 552 258 129
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2036 487
Size: 683 56 810 4473
Fragments arranged by size:
4473 810 683 56
BtsI GCAGTG_nn'
Cuts at: 2478 2772 3538 4332 4352 2478
Size: 294 766 794 20 4148
Fragments arranged by size:
4148 794 766 294 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6022
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5987
DraI TTT'AAA
Cuts at: 2325 3326 2325
Size: 1001 5021
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5029 809
Size: 507 3713 1802
Fragments arranged by size:
3713 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3022 3469 4358
Size: 54 1425 50 275 18 1192 447 889
Cuts at: 4358 5987 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1192 889 447 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3469 1537
Size: 275 1657 4090
Fragments arranged by size:
4090 1657 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2187 3952 1353
Size: 834 1765 3423
Fragments arranged by size:
3423 1765 834
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2369 4609 4983 5129 387
Size: 1064 138 780 2240 374 146 1280
Fragments arranged by size:
2240 1280 1064 780 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2054 2302 2372 2710 2730 2737 4085
Size: 393 422 248 70 338 20 7 1348
Cuts at: 4085 4682 5469 5737 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1348 787 597 422 393 338 268
248 70 20 7
EcoNI CCTnn'n_nnAGG
Cuts at: 2033 2216 2033
Size: 183 5839
EcoO109I rG'GnC_Cy
Cuts at: 2073 2140 2529 2945 3113 3214 2073
Size: 67 389 416 168 101 4881
Fragments arranged by size:
4881 416 389 168 101 67
EcoRI G'AATT_C
Cuts at: 2258 2339 2546 3331 3709 2258
Size: 81 207 785 378 4571
Fragments arranged by size:
4571 785 378 207 81
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3124 3124
Size: 6022
FspI TGC'GCA
Cuts at: 4528 4528
Size: 6022
HaeII r_GCGC'y
Cuts at: 1678 3000 5169 1678
Size: 1322 2169 2531
Fragments arranged by size:
2531 2169 1322
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4663 4695 4737 4769 1373
Size: 32 135 32 3091 32 42 32 2626
Fragments arranged by size:
3091 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3136 3366 3612 669
Size: 988 281 1198 230 246 3079
Fragments arranged by size:
3079 1198 988 281 246 230
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6022
HpaI GTT'AAC
Cuts at: 3612 3612
Size: 6022
KasI G'GCGC_C
Cuts at: 2996 2996
Size: 6022
KpnI G_GTAC'C
Cuts at: 3347 3347
Size: 6022
MfeI C'AATT_G
Cuts at: 3621 3621
Size: 6022
MluI A'CGCG_T
Cuts at: 5898 5898
Size: 6022
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2823 5136 5320 1305
Size: 1518 2313 184 2007
Fragments arranged by size:
2313 2007 1518 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3024 10
Size: 54 1425 343 1192 3008
Fragments arranged by size:
3008 1425 1192 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2021 3980 4339 4498 518
Size: 648 855 1959 359 159 2042
Fragments arranged by size:
2042 1959 855 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2543 3438 4115 5263 5508 1606
Size: 937 895 677 1148 245 2120
Fragments arranged by size:
2120 1148 937 895 677 245
NarI GG'CG_CC
Cuts at: 2997 2997
Size: 6022
NcoI C'CATG_G
Cuts at: 513 1065 2016 2922 513
Size: 552 951 906 3613
Fragments arranged by size:
3613 951 906 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6022
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6022
NotI GC'GGCC_GC
Cuts at: 3469 3469
Size: 6022
NspI r_CATG'y
Cuts at: 1132 1229 3380 4925 1132
Size: 97 2151 1545 2229
Fragments arranged by size:
2229 2151 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6022
PciI A'CATG_T
Cuts at: 4921 4921
Size: 6022
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2655 1108
Size: 1547 4475
PfoI T'CCnGG_A
Cuts at: 2343 2726 2343
Size: 383 5639
PmeI GTTT'AAAC
Cuts at: 3326 3326
Size: 6022
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4091 4123 5604 5636 4091
Size: 32 1481 32 4477
Fragments arranged by size:
4477 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2073 2140 2945 2073
Size: 67 805 5150
Fragments arranged by size:
5150 805 67
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6022
PsiI TTA'TAA
Cuts at: 3592 3592
Size: 6022
PspOMI G'GGCC_C
Cuts at: 2155 2529 3112 2155
Size: 374 583 5065
Fragments arranged by size:
5065 583 374
PstI C_TGCA'G
Cuts at: 830 3374 830
Size: 2544 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6022
SalI G'TCGA_C
Cuts at: 1936 3364 1936
Size: 1428 4594
SanDI GG'GwC_CC
Cuts at: 2945 2945
Size: 6022
SbfI CC_TGCA'GG
Cuts at: 3374 3374
Size: 6022
ScaI AGT'ACT
Cuts at: 4270 5982 4270
Size: 1712 4310
SfcI C'TryA_G
Cuts at: 826 1045 2293 3370 4505 5186 5377 826
Size: 219 1248 1077 1135 681 191 1471
Fragments arranged by size:
1471 1248 1135 1077 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2537 2537
Size: 6022
SfoI GGC'GCC
Cuts at: 2998 2998
Size: 6022
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6022
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6022
SmaI CCC'GGG
Cuts at: 1872 2972 3349 1872
Size: 1100 377 4545
Fragments arranged by size:
4545 1100 377
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3187 4126 5027 5289 820
Size: 197 926 1244 939 901 262 1553
Fragments arranged by size:
1553 1244 939 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3813 493
Size: 3320 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6022
SphI G_CATG'C
Cuts at: 1132 3380 1132
Size: 2248 3774
SrfI GCCC'GGGC
Cuts at: 2972 2972
Size: 6022
SspI AAT'ATT
Cuts at: 5 52 3946 5
Size: 47 3894 2081
Fragments arranged by size:
3894 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6022
StyI C'CwwG_G
Cuts at: 513 1065 2016 2922 3461 513
Size: 552 951 906 539 3074
Fragments arranged by size:
3074 951 906 552 539
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2822 3049 4049 4066 4219 4404 4849
Size: 1255 1242 227 1000 17 153 185 445
Cuts at: 4849 325
Size: 1498
Fragments arranged by size:
1498 1255 1242 1000 445 227 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4268 5980 96
Size: 274 80 33 51 1443 2291 1712 138
Fragments arranged by size:
2291 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2027 2043 2059 2075 2460 2492 5982 6014 2027
Size: 16 16 16 385 32 3490 32 2035
Fragments arranged by size:
3490 2035 385 32 32 16 16 16
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6022
XbaI T'CTAG_A
Cuts at: 3358 3358
Size: 6022
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6022
XmaI C'CCGG_G
Cuts at: 1870 2970 3347 1870
Size: 1100 377 4545
Fragments arranged by size:
4545 1100 377
XmnI GAAnn'nnTTC
Cuts at: 2339 4151 2339
Size: 1812 4210
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3830 276
Size: 53 83 186 936 2296 2468
Fragments arranged by size:
2468 2296 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI
SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PspXI PsrI PvuII RsrII SacI SacII SapI SexAI
SwaI XcmI