Restriction map for
pFN21AE0463
(Circular) (Six-base) MAPSORT of: pFN21AE0463.seq Check: 810 from: 1 to: 5953
pFN21AE0463 5953 bp
With 182 enzymes: SgfI *
October 20, 2009 10:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3763 278
Size: 53 83 186 936 2227 2468
Fragments arranged by size:
2468 2227 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3296 1937
Size: 1359 4594
Acc65I G'GTAC_C
Cuts at: 3274 3274
Size: 5953
AclI AA'CG_TT
Cuts at: 1563 4080 4453 1563
Size: 2517 373 3063
Fragments arranged by size:
3063 2517 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2592 2826 3224 4016 5400 5668 1632
Size: 960 234 398 792 1384 268 1917
Fragments arranged by size:
1917 1384 960 792 398 268 234
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5756
AflIII A'CryG_T
Cuts at: 1203 2638 4852 5829 1203
Size: 1435 2214 977 1327
Fragments arranged by size:
2214 1435 1327 977
AgeI A'CCGG_T
Cuts at: 4784 4784
Size: 5953
AhdI GACnn_n'nnGTC
Cuts at: 4682 4682
Size: 5953
AleI CACnn'nnGTG
Cuts at: 1166 3071 1166
Size: 1905 4048
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5953
AlwNI CAG_nnn'CTG
Cuts at: 2452 2969 3696 5268 2452
Size: 517 727 1572 3137
Fragments arranged by size:
3137 1572 727 517
ApaLI G'TGCA_C
Cuts at: 4010 5166 4010
Size: 1156 4797
ApoI r'AATT_y
Cuts at: 1493 3164 3262 3488 3640 5749 1493
Size: 1671 98 226 152 2109 1697
Fragments arranged by size:
2109 1697 1671 226 152 98
AseI AT'TA_AT
Cuts at: 160 1032 4507 160
Size: 872 3475 1606
Fragments arranged by size:
3475 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5953
AvaI C'yCGr_G
Cuts at: 1870 1943 3099 3278 1870
Size: 73 1156 179 4545
Fragments arranged by size:
4545 1156 179 73
BamHI G'GATC_C
Cuts at: 3283 3650 3283
Size: 367 5586
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3113 3274 4729 618
Size: 325 217 627 1326 161 1455 1842
Fragments arranged by size:
1842 1455 1326 627 325 217 161
BanII G_rGCy'C
Cuts at: 1391 2024 2340 3272 1391
Size: 633 316 932 4072
Fragments arranged by size:
4072 932 633 316
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2512 928
Size: 971 613 4369
Fragments arranged by size:
4369 971 613
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4144 4178 1903
Size: 34 2207 34 3678
Fragments arranged by size:
3678 2207 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3845 5055 3845
Size: 1210 4743
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5953
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3185 4564 136
Size: 107 122 71 631 605 1513 1379 1525
Fragments arranged by size:
1525 1513 1379 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2524 5948 2524
Size: 3424 2529
BlpI GC'TnA_GC
Cuts at: 3370 3370
Size: 5953
Bme1580I G_kGCm'C
Cuts at: 1790 4014 5170 1790
Size: 2224 1156 2573
Fragments arranged by size:
2573 2224 1156
BmgBI CAC'GTC
Cuts at: 1318 2726 1318
Size: 1408 4545
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2247 3684 4642
Size: 416 53 83 399 24 830 1437 958
Cuts at: 4642 442
Size: 1753
Fragments arranged by size:
1753 1437 958 830 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5953
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4613 1239
Size: 3374 2579
Bpu10I CC'TnA_GC
Cuts at: 2844 2844
Size: 5953
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2038 4078 4943 5241 2038
Size: 2040 865 298 2750
Fragments arranged by size:
2750 2040 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3049 4616 882
Size: 617 1550 1567 2219
Fragments arranged by size:
2219 1567 1550 617
BsaAI yAC'GTr
Cuts at: 493 1204 2641 3744 493
Size: 711 1437 1103 2702
Fragments arranged by size:
2702 1437 1103 711
BsaBI GATnn'nnATC
Cuts at: 5947 5947
Size: 5953
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3760 4142 4777 275
Size: 53 83 186 936 2227 382 635 1451
Fragments arranged by size:
2227 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4386 4784 5058 5205 5616 4386
Size: 398 274 147 411 4723
Fragments arranged by size:
4723 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2574 2604 1178
Size: 30 165 30 1171 30 4527
Fragments arranged by size:
4527 1171 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5953
BseYI C'CCAG_C
Cuts at: 3106 5156 5652 3106
Size: 2050 496 3407
Fragments arranged by size:
3407 2050 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5380
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3121 3403 4164 5192 1540
Size: 275 186 1120 282 761 1028 2301
Fragments arranged by size:
2301 1120 1028 761 282 275 186
BsiHKAI G_wGCw'C
Cuts at: 2024 2340 4014 4099 5170 2024
Size: 316 1674 85 1071 2807
Fragments arranged by size:
2807 1674 1071 316 85
BsmI GAATG_Cn'
Cuts at: 3464 3557 3464
Size: 93 5860
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2024 2340 3272 4014 4099 5170 1391
Size: 399 234 316 932 742 85 1071 2174
Fragments arranged by size:
2174 1071 932 742 399 316 234 85
BspEI T'CCGG_A
Cuts at: 5616 5616
Size: 5953
BspHI T'CATG_A
Cuts at: 3842 5572 3842
Size: 1730 4223
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3308 844
Size: 2464 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3225 3840 1394
Size: 429 1402 615 3507
Fragments arranged by size:
3507 1402 615 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4448 4622 65
Size: 1183 3200 174 1396
Fragments arranged by size:
3200 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3208 4597 4784 1700
Size: 110 1398 1389 187 2869
Fragments arranged by size:
2869 1398 1389 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5953
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5953
BssSI C'ACGA_G
Cuts at: 3125 4013 5025 3125
Size: 888 1012 4053
Fragments arranged by size:
4053 1012 888
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5953
BstBI TT'CG_AA
Cuts at: 4757 4757
Size: 5953
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5953
BstYI r'GATC_y
Cuts at: 1972 2524 3283 3650 4035 4052 5504 5590 5602
Size: 552 759 367 385 17 1452 86 12
Cuts at: 5602 5948 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 759 552 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5401
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5214
Fragments arranged by size:
5214 683 56
BtsI GCAGTG_nn'
Cuts at: 2501 3469 4263 4283 2501
Size: 968 794 20 4171
Fragments arranged by size:
4171 968 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5953
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5918
DraI TTT'AAA
Cuts at: 3257 3257
Size: 5953
DraIII CAC_nnn'GTG
Cuts at: 2980 2980
Size: 5953
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4960 809
Size: 507 3644 1802
Fragments arranged by size:
3644 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3400 4289 5918
Size: 54 1425 50 275 18 1570 889 1629
Cuts at: 5918 8
Size: 43
Fragments arranged by size:
1629 1570 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3400 1537
Size: 275 1588 4090
Fragments arranged by size:
4090 1588 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3137 3240 3883 1353
Size: 1784 103 643 3423
Fragments arranged by size:
3423 1784 643 103
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4540 4914 5060 387
Size: 1064 138 2951 374 146 1280
Fragments arranged by size:
2951 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2022 2338 2022
Size: 316 5637
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2592 2826 3224 4016 4613 5400 5668
Size: 393 960 234 398 792 597 787 268
Cuts at: 5668 1239
Size: 1524
Fragments arranged by size:
1524 960 792 787 597 398 393 268
234
EcoNI CCTnn'n_nnAGG
Cuts at: 2345 2345
Size: 5953
EcoO109I rG'GnC_Cy
Cuts at: 3103 3103
Size: 5953
EcoRI G'AATT_C
Cuts at: 3262 3640 3262
Size: 378 5575
FspI TGC'GCA
Cuts at: 4459 4459
Size: 5953
HaeII r_GCGC'y
Cuts at: 1678 5100 1678
Size: 3422 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2102 2134 2301 2333 4594
Size: 32 135 32 530 32 167 32 2261
Cuts at: 4594 4626 4668 4700 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2261 530 167 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3297 3543 669
Size: 988 281 1359 246 3079
Fragments arranged by size:
3079 1359 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5953
HpaI GTT'AAC
Cuts at: 3543 3543
Size: 5953
KpnI G_GTAC'C
Cuts at: 3278 3278
Size: 5953
MfeI C'AATT_G
Cuts at: 2777 3552 2777
Size: 775 5178
MluI A'CGCG_T
Cuts at: 5829 5829
Size: 5953
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5067 5251 1305
Size: 3762 184 2007
Fragments arranged by size:
3762 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4131
Fragments arranged by size:
4131 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2472 2883 3071 3911 4270 4429 518
Size: 648 1306 411 188 840 359 159 2042
Fragments arranged by size:
2042 1306 840 648 411 359 188 159
MspA1I CmG'CkG
Cuts at: 1606 3369 4046 5194 5439 1606
Size: 1763 677 1148 245 2120
Fragments arranged by size:
2120 1763 1148 677 245
NaeI GCC'GGC
Cuts at: 3210 3210
Size: 5953
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5401
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5953
NgoMIV G'CCGG_C
Cuts at: 3208 3208
Size: 5953
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5953
NotI GC'GGCC_GC
Cuts at: 3400 3400
Size: 5953
NspI r_CATG'y
Cuts at: 1132 1229 2816 3311 4856 1132
Size: 97 1587 495 1545 2229
Fragments arranged by size:
2229 1587 1545 495 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5953
PciI A'CATG_T
Cuts at: 4852 4852
Size: 5953
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5953
PfoI T'CCnGG_A
Cuts at: 2340 3137 2340
Size: 797 5156
PmeI GTTT'AAAC
Cuts at: 3257 3257
Size: 5953
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4022 4054 5535 5567 4022
Size: 32 1481 32 4408
Fragments arranged by size:
4408 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3103 3103
Size: 5953
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5953
PsiI TTA'TAA
Cuts at: 2516 3523 2516
Size: 1007 4946
PstI C_TGCA'G
Cuts at: 830 3305 830
Size: 2475 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5953
SacI G_AGCT'C
Cuts at: 2024 2340 2024
Size: 316 5637
SalI G'TCGA_C
Cuts at: 1936 3295 1936
Size: 1359 4594
SanDI GG'GwC_CC
Cuts at: 3103 3103
Size: 5953
SapI GCTCTTCn'nnn_
Cuts at: 3137 3240 3137
Size: 103 5850
SbfI CC_TGCA'GG
Cuts at: 3305 3305
Size: 5953
ScaI AGT'ACT
Cuts at: 2592 4201 5913 2592
Size: 1609 1712 2632
Fragments arranged by size:
2632 1712 1609
SfcI C'TryA_G
Cuts at: 826 1045 3301 4436 5117 5308 826
Size: 219 2256 1135 681 191 1471
Fragments arranged by size:
2256 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5953
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5953
SmaI CCC'GGG
Cuts at: 1872 3101 3280 1872
Size: 1229 179 4545
Fragments arranged by size:
4545 1229 179
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2017 4057 4958 5220 820
Size: 197 926 74 2040 901 262 1553
Fragments arranged by size:
2040 1553 926 901 262 197 74
SnaBI TAC'GTA
Cuts at: 493 3744 493
Size: 3251 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5953
SphI G_CATG'C
Cuts at: 1132 3311 1132
Size: 2179 3774
SspI AAT'ATT
Cuts at: 5 52 3877 5
Size: 47 3825 2081
Fragments arranged by size:
3825 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5953
StyI C'CwwG_G
Cuts at: 513 1065 3392 513
Size: 552 2327 3074
Fragments arranged by size:
3074 2327 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3980 3997 4150 4335 4780 325
Size: 1255 2400 17 153 185 445 1498
Fragments arranged by size:
2400 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2590 4199 5911
Size: 274 80 33 51 1443 613 1609 1712
Cuts at: 5911 96
Size: 138
Fragments arranged by size:
1712 1609 1443 613 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5913 5945 5913
Size: 32 5921
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5953
XbaI T'CTAG_A
Cuts at: 3289 3289
Size: 5953
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5953
XmaI C'CCGG_G
Cuts at: 1870 3099 3278 1870
Size: 1229 179 4545
Fragments arranged by size:
4545 1229 179
XmnI GAAnn'nnTTC
Cuts at: 4082 4082
Size: 5953
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3761 276
Size: 53 83 186 936 2227 2468
Fragments arranged by size:
2468 2227 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SacI SalI SanDI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII
SexAI SfiI SfoI SrfI SwaI XcmI