Restriction map for
pFN21AE0456
(Circular) (Six-base) MAPSORT of: pFN21AE0456.seq Check: 2074 from: 1 to: 5527
pFN21AE0456 5527 bp
With 182 enzymes: SgfI *
October 20, 2009 10:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3337 278
Size: 53 83 186 936 1801 2468
Fragments arranged by size:
2468 1801 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2870 1937
Size: 933 4594
Acc65I G'GTAC_C
Cuts at: 2848 2848
Size: 5527
AclI AA'CG_TT
Cuts at: 1563 3654 4027 1563
Size: 2091 373 3063
Fragments arranged by size:
3063 2091 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3590 4974 5242 1632
Size: 1958 1384 268 1917
Fragments arranged by size:
1958 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5330
AflIII A'CryG_T
Cuts at: 1203 4426 5403 1203
Size: 3223 977 1327
Fragments arranged by size:
3223 1327 977
AgeI A'CCGG_T
Cuts at: 4358 4358
Size: 5527
AhdI GACnn_n'nnGTC
Cuts at: 4256 4256
Size: 5527
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5527
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5527
AlwNI CAG_nnn'CTG
Cuts at: 2080 3270 4842 2080
Size: 1190 1572 2765
Fragments arranged by size:
2765 1572 1190
ApaI G_GGCC'C
Cuts at: 2587 2587
Size: 5527
ApaLI G'TGCA_C
Cuts at: 3584 4740 3584
Size: 1156 4371
ApoI r'AATT_y
Cuts at: 1493 2836 3062 3214 5323 1493
Size: 1343 226 152 2109 1697
Fragments arranged by size:
2109 1697 1343 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4081 160
Size: 872 3049 1606
Fragments arranged by size:
3049 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5527
AvaI C'yCGr_G
Cuts at: 1870 1943 2347 2852 1870
Size: 73 404 505 4545
Fragments arranged by size:
4545 505 404 73
BamHI G'GATC_C
Cuts at: 2857 3224 2857
Size: 367 5160
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2652 2848 4303 618
Size: 325 217 627 865 196 1455 1842
Fragments arranged by size:
1842 1455 865 627 325 217 196
BanII G_rGCy'C
Cuts at: 1391 2347 2587 2846 1391
Size: 956 240 259 4072
Fragments arranged by size:
4072 956 259 240
BbeI G_GCGC'C
Cuts at: 2656 2656
Size: 5527
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4556
BbvCI CC'TCA_GC
Cuts at: 2736 2736
Size: 5527
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2497 2531 3718 3752 1903
Size: 34 50 34 476 34 1187 34 3678
Fragments arranged by size:
3678 1187 476 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3419 4629 3419
Size: 1210 4317
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5527
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2156 2661 2816
Size: 107 122 71 631 605 484 505 155
Cuts at: 2816 4138 136
Size: 1322 1525
Fragments arranged by size:
1525 1322 631 605 505 484 155 122
107 71
BglII A'GATC_T
Cuts at: 5522 5522
Size: 5527
BlpI GC'TnA_GC
Cuts at: 2597 2944 2597
Size: 347 5180
Bme1580I G_kGCm'C
Cuts at: 1790 2253 2587 2685 3588 4744 1790
Size: 463 334 98 903 1156 2573
Fragments arranged by size:
2573 1156 903 463 334 98
BmgBI CAC'GTC
Cuts at: 1318 2704 1318
Size: 1386 4141
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2650 3258 4216
Size: 416 53 83 399 24 1233 608 958
Cuts at: 4216 442
Size: 1753
Fragments arranged by size:
1753 1233 958 608 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5527
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2057 4187 1239
Size: 818 2130 2579
Fragments arranged by size:
2579 2130 818
Bpu10I CC'TnA_GC
Cuts at: 2736 2736
Size: 5527
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3652 4517 4815 3652
Size: 865 298 4364
Fragments arranged by size:
4364 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4190 882
Size: 617 2691 2219
Fragments arranged by size:
2691 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2358 3318 493
Size: 711 1154 960 2702
Fragments arranged by size:
2702 1154 960 711
BsaBI GATnn'nnATC
Cuts at: 2537 5521 2537
Size: 2984 2543
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2653 3334 3716 4351
Size: 53 83 186 936 1120 681 382 635
Cuts at: 4351 275
Size: 1451
Fragments arranged by size:
1451 1120 936 681 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3960 4358 4632 4779 5190 3960
Size: 398 274 147 411 4297
Fragments arranged by size:
4297 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2037 2067 2165 2195 1178
Size: 30 165 30 634 30 98 30 4510
Fragments arranged by size:
4510 634 165 98 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2045 2114 1186
Size: 859 69 4599
Fragments arranged by size:
4599 859 69
BseYI C'CCAG_C
Cuts at: 2085 2094 4730 5226 2085
Size: 9 2636 496 2386
Fragments arranged by size:
2636 2386 496 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2366 2552 1163
Size: 573 630 186 4138
Fragments arranged by size:
4138 630 573 186
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2801 2977 3738 4766 1540
Size: 275 186 800 176 761 1028 2301
Fragments arranged by size:
2301 1028 800 761 275 186 176
BsiHKAI G_wGCw'C
Cuts at: 2406 3588 3673 4744 2406
Size: 1182 85 1071 3189
Fragments arranged by size:
3189 1182 1071 85
BsmI GAATG_Cn'
Cuts at: 3038 3131 3038
Size: 93 5434
BsmBI CGTCTCn'nnnn_
Cuts at: 2185 2185
Size: 5527
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2253 2347 2406 2587 2685 2846 3588
Size: 399 463 94 59 181 98 161 742
Cuts at: 3588 3673 4744 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 463 399 181 161 98
94 85 59
BspEI T'CCGG_A
Cuts at: 5190 5190
Size: 5527
BspHI T'CATG_A
Cuts at: 3416 5146 3416
Size: 1730 3797
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2388 2882 844
Size: 1544 494 3489
Fragments arranged by size:
3489 1544 494
BsrBI CCG'CTC
Cuts at: 1394 1823 2336 2793 3414 1394
Size: 429 513 457 621 3507
Fragments arranged by size:
3507 621 513 457 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4022 4196 65
Size: 1183 2774 174 1396
Fragments arranged by size:
2774 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2168 2292 2660 4171 4358 1700
Size: 110 358 124 368 1511 187 2869
Fragments arranged by size:
2869 1511 368 358 187 124 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5527
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5527
BssSI C'ACGA_G
Cuts at: 3587 4599 3587
Size: 1012 4515
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2563 794
Size: 1769 3758
BstBI TT'CG_AA
Cuts at: 4331 4331
Size: 5527
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5527
BstXI CCAn_nnnn'nTGG
Cuts at: 2425 2425
Size: 5527
BstYI r'GATC_y
Cuts at: 1972 2532 2857 3224 3609 3626 5078 5164 5176
Size: 560 325 367 385 17 1452 86 12
Cuts at: 5176 5522 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 560 385 367 346 325 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2418 2764 513
Size: 552 1353 346 3276
Fragments arranged by size:
3276 1353 552 346
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4788
Fragments arranged by size:
4788 683 56
BtsI GCAGTG_nn'
Cuts at: 2163 2561 3043 3837 3857 2163
Size: 398 482 794 20 3833
Fragments arranged by size:
3833 794 482 398 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5527
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5492
DraI TTT'AAA
Cuts at: 2831 2831
Size: 5527
DraIII CAC_nnn'GTG
Cuts at: 2575 2575
Size: 5527
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4534 809
Size: 507 3218 1802
Fragments arranged by size:
3218 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2022 2337 2676
Size: 54 1425 50 275 18 192 315 339
Cuts at: 2676 2688 2798 2974 3863 5492 8
Size: 12 110 176 889 1629 43
Fragments arranged by size:
1629 1425 889 339 315 275 192 176
110 54 50 43 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 2798 2974 1537
Size: 275 986 176 4090
Fragments arranged by size:
4090 986 275 176
EarI CTCTTCn'nnn_
Cuts at: 1353 2054 2786 3457 1353
Size: 701 732 671 3423
Fragments arranged by size:
3423 732 701 671
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4114 4488 4634 387
Size: 1064 138 2525 374 146 1280
Fragments arranged by size:
2525 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2057 3590 4187 4974 5242 1239
Size: 393 425 1533 597 787 268 1524
Fragments arranged by size:
1533 1524 787 597 425 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2384 2384
Size: 5527
EcoO109I rG'GnC_Cy
Cuts at: 2583 2584 2769 2583
Size: 1 185 5341
Fragments arranged by size:
5341 185 1
EcoRI G'AATT_C
Cuts at: 2836 3214 2836
Size: 378 5149
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2488 2488
Size: 5527
FspI TGC'GCA
Cuts at: 4033 4033
Size: 5527
HaeII r_GCGC'y
Cuts at: 1678 2656 2718 4674 1678
Size: 978 62 1956 2531
Fragments arranged by size:
2531 1956 978 62
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2037 2069 4168 4200 4242
Size: 32 135 32 465 32 2099 32 42
Cuts at: 4242 4274 1373
Size: 32 2626
Fragments arranged by size:
2626 2099 465 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2707 2871 3117 669
Size: 988 281 769 164 246 3079
Fragments arranged by size:
3079 988 769 281 246 164
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5527
HpaI GTT'AAC
Cuts at: 3117 3117
Size: 5527
KasI G'GCGC_C
Cuts at: 2652 2652
Size: 5527
KpnI G_GTAC'C
Cuts at: 2852 2852
Size: 5527
MfeI C'AATT_G
Cuts at: 3126 3126
Size: 5527
MluI A'CGCG_T
Cuts at: 5403 5403
Size: 5527
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4641 4825 1305
Size: 3336 184 2007
Fragments arranged by size:
3336 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2690 10
Size: 54 1425 343 858 2847
Fragments arranged by size:
2847 1425 858 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2492 3485 3844 4003 518
Size: 648 1326 993 359 159 2042
Fragments arranged by size:
2042 1326 993 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2077 2943 3620 4768 5013 1606
Size: 471 866 677 1148 245 2120
Fragments arranged by size:
2120 1148 866 677 471 245
NaeI GCC'GGC
Cuts at: 2170 2662 2170
Size: 492 5035
NarI GG'CG_CC
Cuts at: 2653 2653
Size: 5527
NcoI C'CATG_G
Cuts at: 513 1065 2418 513
Size: 552 1353 3622
Fragments arranged by size:
3622 1353 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5527
NgoMIV G'CCGG_C
Cuts at: 2168 2660 2168
Size: 492 5035
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5527
NotI GC'GGCC_GC
Cuts at: 2974 2974
Size: 5527
NspI r_CATG'y
Cuts at: 1132 1229 2491 2885 4430 1132
Size: 97 1262 394 1545 2229
Fragments arranged by size:
2229 1545 1262 394 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5527
PciI A'CATG_T
Cuts at: 4426 4426
Size: 5527
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5527
PmeI GTTT'AAAC
Cuts at: 2831 2831
Size: 5527
PmlI CAC'GTG
Cuts at: 2358 2358
Size: 5527
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3596 3628 5109 5141 3596
Size: 32 1481 32 3982
Fragments arranged by size:
3982 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5527
PsiI TTA'TAA
Cuts at: 3097 3097
Size: 5527
PspOMI G'GGCC_C
Cuts at: 2583 2583
Size: 5527
PstI C_TGCA'G
Cuts at: 830 2879 830
Size: 2049 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5527
SalI G'TCGA_C
Cuts at: 1936 2869 1936
Size: 933 4594
SbfI CC_TGCA'GG
Cuts at: 2879 2879
Size: 5527
ScaI AGT'ACT
Cuts at: 3775 5487 3775
Size: 1712 3815
SexAI A'CCwGG_T
Cuts at: 2304 2304
Size: 5527
SfcI C'TryA_G
Cuts at: 826 1045 2875 4010 4691 4882 826
Size: 219 1830 1135 681 191 1471
Fragments arranged by size:
1830 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2156 2816 2156
Size: 660 4867
SfoI GGC'GCC
Cuts at: 2654 2654
Size: 5527
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5527
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5527
SmaI CCC'GGG
Cuts at: 1872 2349 2854 1872
Size: 477 505 4545
Fragments arranged by size:
4545 505 477
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3631 4532 4794 820
Size: 197 926 1688 901 262 1553
Fragments arranged by size:
1688 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3318 493
Size: 2825 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5527
SphI G_CATG'C
Cuts at: 1132 2885 1132
Size: 1753 3774
SspI AAT'ATT
Cuts at: 5 52 3451 5
Size: 47 3399 2081
Fragments arranged by size:
3399 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5527
StyI C'CwwG_G
Cuts at: 513 1065 2418 2524 2966 513
Size: 552 1353 106 442 3074
Fragments arranged by size:
3074 1353 552 442 106
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3554 3571 3724 3909 4354 325
Size: 1255 1974 17 153 185 445 1498
Fragments arranged by size:
1974 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2544 2637 3773
Size: 274 80 33 51 1443 567 93 1136
Cuts at: 3773 5485 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1136 567 274 138 93 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2207 2239 5487 5519 2207
Size: 32 3248 32 2215
Fragments arranged by size:
3248 2215 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2111 1316
Size: 795 4732
XbaI T'CTAG_A
Cuts at: 2863 2863
Size: 5527
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5527
XmaI C'CCGG_G
Cuts at: 1870 2347 2852 1870
Size: 477 505 4545
Fragments arranged by size:
4545 505 477
XmnI GAAnn'nnTTC
Cuts at: 3656 3656
Size: 5527
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3335 276
Size: 53 83 186 936 1801 2468
Fragments arranged by size:
2468 1801 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspOMI PstI PvuI SalI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI NruI
NsiI PacI PfoI PpuMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SrfI SwaI XcmI