Restriction map for
pFN21AE0454
(Circular) (Six-base) MAPSORT of: pFN21AE0454.seq Check: 5223 from: 1 to: 5587
pFN21AE0454 5587 bp
With 182 enzymes: SgfI *
October 20, 2009 10:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2391 2391
Size: 5587
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3397 278
Size: 53 83 186 936 1861 2468
Fragments arranged by size:
2468 1861 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2930 1937
Size: 993 4594
Acc65I G'GTAC_C
Cuts at: 2908 2908
Size: 5587
AclI AA'CG_TT
Cuts at: 1563 3714 4087 1563
Size: 2151 373 3063
Fragments arranged by size:
3063 2151 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3650 5034 5302 1632
Size: 2018 1384 268 1917
Fragments arranged by size:
2018 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2650 820
Size: 197 1633 3757
Fragments arranged by size:
3757 1633 197
AflIII A'CryG_T
Cuts at: 1203 2616 4486 5463 1203
Size: 1413 1870 977 1327
Fragments arranged by size:
1870 1413 1327 977
AgeI A'CCGG_T
Cuts at: 2127 4418 2127
Size: 2291 3296
AhdI GACnn_n'nnGTC
Cuts at: 4316 4316
Size: 5587
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5587
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5587
AlwNI CAG_nnn'CTG
Cuts at: 3330 4902 3330
Size: 1572 4015
ApaI G_GGCC'C
Cuts at: 2036 2036
Size: 5587
ApaLI G'TGCA_C
Cuts at: 3644 4800 3644
Size: 1156 4431
ApoI r'AATT_y
Cuts at: 1493 2532 2600 2663 2810 2896 3122 3274 5383
Size: 1039 68 63 147 86 226 152 2109
Cuts at: 5383 1493
Size: 1697
Fragments arranged by size:
2109 1697 1039 226 152 147 86 68
63
AseI AT'TA_AT
Cuts at: 160 1032 4141 160
Size: 872 3109 1606
Fragments arranged by size:
3109 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5587
AvaI C'yCGr_G
Cuts at: 1870 1943 2414 2912 1870
Size: 73 471 498 4545
Fragments arranged by size:
4545 498 471 73
BamHI G'GATC_C
Cuts at: 2152 2917 3284 2152
Size: 765 367 4455
Fragments arranged by size:
4455 765 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2908 4363 618
Size: 325 217 627 1121 1455 1842
Fragments arranged by size:
1842 1455 1121 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2036 2906 1391
Size: 645 870 4072
Fragments arranged by size:
4072 870 645
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2808 2835 928
Size: 971 909 27 3680
Fragments arranged by size:
3680 971 909 27
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3778 3812 1903
Size: 34 50 34 1757 34 3678
Fragments arranged by size:
3678 1757 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3479 4689 3479
Size: 1210 4377
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5587
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2040 4198 136
Size: 107 122 71 631 605 368 2158 1525
Fragments arranged by size:
2158 1525 631 605 368 122 107 71
BglII A'GATC_T
Cuts at: 5582 5582
Size: 5587
BlpI GC'TnA_GC
Cuts at: 3004 3004
Size: 5587
Bme1580I G_kGCm'C
Cuts at: 1790 2036 3648 4804 1790
Size: 246 1612 1156 2573
Fragments arranged by size:
2573 1612 1156 246
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5587
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3318 4276 442
Size: 416 53 83 399 24 1901 958 1753
Fragments arranged by size:
1901 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5587
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2863 2863
Size: 5587
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2327 4247 1239
Size: 1088 1920 2579
Fragments arranged by size:
2579 1920 1088
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3712 4577 4875 3712
Size: 865 298 4424
Fragments arranged by size:
4424 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4250 882
Size: 617 2751 2219
Fragments arranged by size:
2751 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3378 493
Size: 711 2174 2702
Fragments arranged by size:
2702 2174 711
BsaBI GATnn'nnATC
Cuts at: 5581 5581
Size: 5587
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3394 3776 4411 275
Size: 53 83 186 936 1861 382 635 1451
Fragments arranged by size:
1861 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2127 4020 4418 4692 4839 5250 2127
Size: 1893 398 274 147 411 2464
Fragments arranged by size:
2464 1893 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2250 2280 1178
Size: 30 165 30 847 30 4485
Fragments arranged by size:
4485 847 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5587
BseYI C'CCAG_C
Cuts at: 4790 5286 4790
Size: 496 5091
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5014
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3037 3798 4826 1540
Size: 275 186 1036 761 1028 2301
Fragments arranged by size:
2301 1036 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3648 3733 4804 3648
Size: 85 1071 4431
Fragments arranged by size:
4431 1071 85
BsmI GAATG_Cn'
Cuts at: 2879 3098 3191 2879
Size: 219 93 5275
Fragments arranged by size:
5275 219 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2036 2906 3648 3733 4804 1391
Size: 399 246 870 742 85 1071 2174
Fragments arranged by size:
2174 1071 870 742 399 246 85
BspEI T'CCGG_A
Cuts at: 5250 5250
Size: 5587
BspHI T'CATG_A
Cuts at: 2550 3476 5206 2550
Size: 926 1730 2931
Fragments arranged by size:
2931 1730 926
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2391 2942 844
Size: 1547 551 3489
Fragments arranged by size:
3489 1547 551
BsrBI CCG'CTC
Cuts at: 1394 1823 3474 1394
Size: 429 1651 3507
Fragments arranged by size:
3507 1651 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4082 4256 65
Size: 1183 2834 174 1396
Fragments arranged by size:
2834 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2127 4231 4418 1700
Size: 110 317 2104 187 2869
Fragments arranged by size:
2869 2104 317 187 110
BsrGI T'GTAC_A
Cuts at: 96 2373 96
Size: 2277 3310
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5587
BssSI C'ACGA_G
Cuts at: 3647 4659 3647
Size: 1012 4575
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5587
BstBI TT'CG_AA
Cuts at: 4391 4391
Size: 5587
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5587
BstYI r'GATC_y
Cuts at: 1972 2058 2152 2563 2608 2917 3284 3669 3686
Size: 86 94 411 45 309 367 385 17
Cuts at: 3686 5138 5224 5236 5582 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 411 385 367 346 309 94
86 86 45 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2037 2100 513
Size: 552 972 63 4000
Fragments arranged by size:
4000 972 552 63
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4848
Fragments arranged by size:
4848 683 56
BtsI GCAGTG_nn'
Cuts at: 2426 3103 3897 3917 2426
Size: 677 794 20 4096
Fragments arranged by size:
4096 794 677 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5587
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5552
DraI TTT'AAA
Cuts at: 2815 2891 2815
Size: 76 5511
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4594 809
Size: 507 3278 1802
Fragments arranged by size:
3278 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2320 3034 3923
Size: 54 1425 50 275 18 490 714 889
Cuts at: 3923 5552 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 714 490 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3034 1537
Size: 275 1222 4090
Fragments arranged by size:
4090 1222 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3517 1353
Size: 2164 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4174 4548 4694 387
Size: 1064 138 2585 374 146 1280
Fragments arranged by size:
2585 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2327 3650 4247 5034 5302 1239
Size: 393 695 1323 597 787 268 1524
Fragments arranged by size:
1524 1323 787 695 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2033 2033
Size: 5587
EcoRI G'AATT_C
Cuts at: 2896 3274 2896
Size: 378 5209
FspI TGC'GCA
Cuts at: 2019 4093 2019
Size: 2074 3513
HaeII r_GCGC'y
Cuts at: 1678 4734 1678
Size: 3056 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4228 4260 4302 4334 1373
Size: 32 135 32 2656 32 42 32 2626
Fragments arranged by size:
2656 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2545 2931 3177 669
Size: 988 281 607 386 246 3079
Fragments arranged by size:
3079 988 607 386 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5587
HpaI GTT'AAC
Cuts at: 3177 3177
Size: 5587
KpnI G_GTAC'C
Cuts at: 2912 2912
Size: 5587
MfeI C'AATT_G
Cuts at: 3186 3186
Size: 5587
MluI A'CGCG_T
Cuts at: 5463 5463
Size: 5587
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4701 4885 1305
Size: 3396 184 2007
Fragments arranged by size:
3396 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2322 10
Size: 54 1425 343 490 3275
Fragments arranged by size:
3275 1425 490 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3545 3904 4063 518
Size: 648 2379 359 159 2042
Fragments arranged by size:
2379 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2023 2039 2046 2401 3003 3680 4828 5073
Size: 417 16 7 355 602 677 1148 245
Cuts at: 5073 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 602 417 355 245 16
7
NcoI C'CATG_G
Cuts at: 513 1065 2100 513
Size: 552 1035 4000
Fragments arranged by size:
4000 1035 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5587
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5587
NotI GC'GGCC_GC
Cuts at: 3034 3034
Size: 5587
NspI r_CATG'y
Cuts at: 1132 1229 2620 2945 4490 1132
Size: 97 1391 325 1545 2229
Fragments arranged by size:
2229 1545 1391 325 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5587
PciI A'CATG_T
Cuts at: 2616 4486 2616
Size: 1870 3717
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5587
PfoI T'CCnGG_A
Cuts at: 2196 2196
Size: 5587
PmeI GTTT'AAAC
Cuts at: 2891 2891
Size: 5587
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3656 3688 5169 5201 3656
Size: 32 1481 32 4042
Fragments arranged by size:
4042 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5587
PsiI TTA'TAA
Cuts at: 3157 3157
Size: 5587
PspOMI G'GGCC_C
Cuts at: 2032 2032
Size: 5587
PspXI vC'TCGA_Gb
Cuts at: 2414 2414
Size: 5587
PstI C_TGCA'G
Cuts at: 830 2939 830
Size: 2109 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5587
PvuII CAG'CTG
Cuts at: 2023 2401 2023
Size: 378 5209
SacII CC_GC'GG
Cuts at: 2040 2040
Size: 5587
SalI G'TCGA_C
Cuts at: 1936 2929 1936
Size: 993 4594
SbfI CC_TGCA'GG
Cuts at: 2939 2939
Size: 5587
ScaI AGT'ACT
Cuts at: 2282 3835 5547 2282
Size: 1553 1712 2322
Fragments arranged by size:
2322 1712 1553
SfcI C'TryA_G
Cuts at: 826 1045 2707 2935 4070 4751 4942 826
Size: 219 1662 228 1135 681 191 1471
Fragments arranged by size:
1662 1471 1135 681 228 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5587
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5587
SmaI CCC'GGG
Cuts at: 1872 2914 1872
Size: 1042 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2414 2650 3691 4592 4854 820
Size: 197 926 471 236 1041 901 262 1553
Fragments arranged by size:
1553 1041 926 901 471 262 236 197
SnaBI TAC'GTA
Cuts at: 493 3378 493
Size: 2885 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5587
SphI G_CATG'C
Cuts at: 1132 2945 1132
Size: 1813 3774
SspI AAT'ATT
Cuts at: 5 52 2864 3511 5
Size: 47 2812 647 2081
Fragments arranged by size:
2812 2081 647 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5587
StyI C'CwwG_G
Cuts at: 513 1065 2100 3026 513
Size: 552 1035 926 3074
Fragments arranged by size:
3074 1035 926 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2237 3614 3631 3784 3969 4414 325
Size: 1255 657 1377 17 153 185 445 1498
Fragments arranged by size:
1498 1377 1255 657 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2280 2373 3833
Size: 274 80 33 51 1443 303 93 1460
Cuts at: 3833 5545 96
Size: 1712 138
Fragments arranged by size:
1712 1460 1443 303 274 138 93 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5547 5579 5547
Size: 32 5555
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5587
XbaI T'CTAG_A
Cuts at: 2527 2923 2527
Size: 396 5191
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2260 2260
Size: 5587
XhoI C'TCGA_G
Cuts at: 1943 2414 1943
Size: 471 5116
XmaI C'CCGG_G
Cuts at: 1870 2912 1870
Size: 1042 4545
XmnI GAAnn'nnTTC
Cuts at: 2865 3716 2865
Size: 851 4736
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3395 276
Size: 53 83 186 936 1861 2468
Fragments arranged by size:
2468 1861 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SacII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PsrI RsrII
SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI