Restriction map for
pFN21AE0452
(Circular) (Six-base) MAPSORT of: pFN21AE0452.seq Check: 1434 from: 1 to: 6091
pFN21AE0452 6091 bp
With 182 enzymes: SgfI *
October 20, 2009 10:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3901 278
Size: 53 83 186 936 2365 2468
Fragments arranged by size:
2468 2365 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3434 1937
Size: 1497 4594
Acc65I G'GTAC_C
Cuts at: 2568 2617 3412 2568
Size: 49 795 5247
Fragments arranged by size:
5247 795 49
AclI AA'CG_TT
Cuts at: 1563 4218 4591 1563
Size: 2655 373 3063
Fragments arranged by size:
3063 2655 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3158 4154 5538 5806 1632
Size: 1526 996 1384 268 1917
Fragments arranged by size:
1917 1526 1384 996 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5894
AflIII A'CryG_T
Cuts at: 1203 2314 2703 4990 5967 1203
Size: 1111 389 2287 977 1327
Fragments arranged by size:
2287 1327 1111 977 389
AgeI A'CCGG_T
Cuts at: 4922 4922
Size: 6091
AhdI GACnn_n'nnGTC
Cuts at: 4820 4820
Size: 6091
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6091
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6091
AlwNI CAG_nnn'CTG
Cuts at: 3834 5406 3834
Size: 1572 4519
ApaI G_GGCC'C
Cuts at: 3077 3077
Size: 6091
ApaLI G'TGCA_C
Cuts at: 4148 5304 4148
Size: 1156 4935
ApoI r'AATT_y
Cuts at: 1493 2176 2227 2961 3400 3626 3778 5887 1493
Size: 683 51 734 439 226 152 2109 1697
Fragments arranged by size:
2109 1697 734 683 439 226 152 51
AseI AT'TA_AT
Cuts at: 160 1032 4645 160
Size: 872 3613 1606
Fragments arranged by size:
3613 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6091
AvaI C'yCGr_G
Cuts at: 1870 1943 2493 3416 1870
Size: 73 550 923 4545
Fragments arranged by size:
4545 923 550 73
BamHI G'GATC_C
Cuts at: 2497 3421 3788 2497
Size: 924 367 4800
Fragments arranged by size:
4800 924 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2568 2617 3412 4867 618
Size: 325 217 627 781 49 795 1455 1842
Fragments arranged by size:
1842 1455 795 781 627 325 217 49
BanII G_rGCy'C
Cuts at: 1391 2119 3077 3410 1391
Size: 728 958 333 4072
Fragments arranged by size:
4072 958 728 333
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2780 928
Size: 971 881 4239
Fragments arranged by size:
4239 971 881
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3104 3138 4282 4316 1903
Size: 34 1167 34 1144 34 3678
Fragments arranged by size:
3678 1167 1144 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3074 3983 5193 3074
Size: 909 1210 3972
Fragments arranged by size:
3972 1210 909
BclI T'GATC_A
Cuts at: 1548 2695 1548
Size: 1147 4944
BfrBI ATG'CAT
Cuts at: 3365 3365
Size: 6091
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4702 136
Size: 107 122 71 631 605 3030 1525
Fragments arranged by size:
3030 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6086 6086
Size: 6091
BlpI GC'TnA_GC
Cuts at: 2536 3508 2536
Size: 972 5119
Bme1580I G_kGCm'C
Cuts at: 1790 3077 4152 5308 1790
Size: 1287 1075 1156 2573
Fragments arranged by size:
2573 1287 1156 1075
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6091
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3822 4780 442
Size: 416 53 83 399 24 2405 958 1753
Fragments arranged by size:
2405 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3024 1056
Size: 1968 4123
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2427 2443 4751 1239
Size: 1188 16 2308 2579
Fragments arranged by size:
2579 2308 1188 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4216 5081 5379 4216
Size: 865 298 4928
Fragments arranged by size:
4928 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2086 2868 4754 882
Size: 617 587 782 1886 2219
Fragments arranged by size:
2219 1886 782 617 587
BsaAI yAC'GTr
Cuts at: 493 1204 3882 493
Size: 711 2678 2702
Fragments arranged by size:
2702 2678 711
BsaBI GATnn'nnATC
Cuts at: 6085 6085
Size: 6091
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3898 4280 4915 275
Size: 53 83 186 936 2365 382 635 1451
Fragments arranged by size:
2365 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4524 4922 5196 5343 5754 4524
Size: 398 274 147 411 4861
Fragments arranged by size:
4861 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2441 2471 1178
Size: 30 165 30 1038 30 4798
Fragments arranged by size:
4798 1038 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6091
BseYI C'CCAG_C
Cuts at: 3185 5294 5790 3185
Size: 2109 496 3486
Fragments arranged by size:
3486 2109 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5518
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3541 4302 5330 1540
Size: 275 186 1540 761 1028 2301
Fragments arranged by size:
2301 1540 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2119 4152 4237 5308 2119
Size: 2033 85 1071 2902
Fragments arranged by size:
2902 2033 1071 85
BsmI GAATG_Cn'
Cuts at: 3602 3695 3602
Size: 93 5998
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2119 3077 3410 4152 4237 5308 1391
Size: 399 329 958 333 742 85 1071 2174
Fragments arranged by size:
2174 1071 958 742 399 333 329 85
BspEI T'CCGG_A
Cuts at: 5754 5754
Size: 6091
BspHI T'CATG_A
Cuts at: 3980 5710 3980
Size: 1730 4361
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3446 844
Size: 2602 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3978 1394
Size: 429 2155 3507
Fragments arranged by size:
3507 2155 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2788 4586 4760 65
Size: 1183 1540 1798 174 1396
Fragments arranged by size:
1798 1540 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4735 4922 1700
Size: 110 2925 187 2869
Fragments arranged by size:
2925 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2706 96
Size: 2610 3481
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6091
BssSI C'ACGA_G
Cuts at: 4151 5163 4151
Size: 1012 5079
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3362 794
Size: 2568 3523
BstBI TT'CG_AA
Cuts at: 4895 4895
Size: 6091
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6091
BstXI CCAn_nnnn'nTGG
Cuts at: 2629 2629
Size: 6091
BstYI r'GATC_y
Cuts at: 1972 2497 3421 3788 4173 4190 5642 5728 5740
Size: 525 924 367 385 17 1452 86 12
Cuts at: 5740 6086 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 924 525 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2857 2857
Size: 6091
BtgI C'CryG_G
Cuts at: 513 1065 2004 3104 513
Size: 552 939 1100 3500
Fragments arranged by size:
3500 1100 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5352
Fragments arranged by size:
5352 683 56
BtsI GCAGTG_nn'
Cuts at: 3607 4401 4421 3607
Size: 794 20 5277
Fragments arranged by size:
5277 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6091
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6056
DraI TTT'AAA
Cuts at: 2082 2232 3395 2082
Size: 150 1163 4778
Fragments arranged by size:
4778 1163 150
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5098 809
Size: 507 3782 1802
Fragments arranged by size:
3782 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3538 4427 6056
Size: 54 1425 50 275 18 1708 889 1629
Cuts at: 6056 8
Size: 43
Fragments arranged by size:
1708 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3538 1537
Size: 275 1726 4090
Fragments arranged by size:
4090 1726 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2793 4021 1353
Size: 1440 1228 3423
Fragments arranged by size:
3423 1440 1228
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4678 5052 5198 387
Size: 1064 138 3089 374 146 1280
Fragments arranged by size:
3089 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2117 2117
Size: 6091
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2427 2443 3158 4154 4751 5538 5806
Size: 393 795 16 715 996 597 787 268
Cuts at: 5806 1239
Size: 1524
Fragments arranged by size:
1524 996 795 787 715 597 393 268
16
EcoNI CCTnn'n_nnAGG
Cuts at: 2758 2758
Size: 6091
EcoO109I rG'GnC_Cy
Cuts at: 2398 2398
Size: 6091
EcoRI G'AATT_C
Cuts at: 3400 3778 3400
Size: 378 5713
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3375 3375
Size: 6091
FspI TGC'GCA
Cuts at: 4597 4597
Size: 6091
HaeII r_GCGC'y
Cuts at: 1678 5238 1678
Size: 3560 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2441 2473 4732 4764 4806
Size: 32 135 32 869 32 2259 32 42
Cuts at: 4806 4838 1373
Size: 32 2626
Fragments arranged by size:
2626 2259 869 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2850 3435 3681 669
Size: 988 281 912 585 246 3079
Fragments arranged by size:
3079 988 912 585 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6091
HpaI GTT'AAC
Cuts at: 2850 3681 2850
Size: 831 5260
KpnI G_GTAC'C
Cuts at: 2572 2621 3416 2572
Size: 49 795 5247
Fragments arranged by size:
5247 795 49
MfeI C'AATT_G
Cuts at: 2544 3690 2544
Size: 1146 4945
MluI A'CGCG_T
Cuts at: 5967 5967
Size: 6091
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5205 5389 1305
Size: 3900 184 2007
Fragments arranged by size:
3900 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4269
Fragments arranged by size:
4269 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2931 4049 4408 4567 518
Size: 648 1765 1118 359 159 2042
Fragments arranged by size:
2042 1765 1118 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3379 3507 4184 5332 5577 1606
Size: 1773 128 677 1148 245 2120
Fragments arranged by size:
2120 1773 1148 677 245 128
NcoI C'CATG_G
Cuts at: 513 1065 2004 3104 513
Size: 552 939 1100 3500
Fragments arranged by size:
3500 1100 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6091
NheI G'CTAG_C
Cuts at: 1052 3020 1052
Size: 1968 4123
NotI GC'GGCC_GC
Cuts at: 3538 3538
Size: 6091
NsiI A_TGCA'T
Cuts at: 3367 3367
Size: 6091
NspI r_CATG'y
Cuts at: 1132 1229 2318 2707 3449 4994 1132
Size: 97 1089 389 742 1545 2229
Fragments arranged by size:
2229 1545 1089 742 389 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6091
PciI A'CATG_T
Cuts at: 2314 2703 4990 2314
Size: 389 2287 3415
Fragments arranged by size:
3415 2287 389
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6091
PmeI GTTT'AAAC
Cuts at: 3395 3395
Size: 6091
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4160 4192 5673 5705 4160
Size: 32 1481 32 4546
Fragments arranged by size:
4546 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2398 2398
Size: 6091
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6091
PsiI TTA'TAA
Cuts at: 3661 3661
Size: 6091
PspOMI G'GGCC_C
Cuts at: 3073 3073
Size: 6091
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3250 3282 3250
Size: 32 6059
PstI C_TGCA'G
Cuts at: 830 3443 830
Size: 2613 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6091
PvuII CAG'CTG
Cuts at: 3379 3379
Size: 6091
SacI G_AGCT'C
Cuts at: 2119 2119
Size: 6091
SalI G'TCGA_C
Cuts at: 1936 3433 1936
Size: 1497 4594
SanDI GG'GwC_CC
Cuts at: 2398 2398
Size: 6091
SapI GCTCTTCn'nnn_
Cuts at: 2793 2793
Size: 6091
SbfI CC_TGCA'GG
Cuts at: 3443 3443
Size: 6091
ScaI AGT'ACT
Cuts at: 4339 6051 4339
Size: 1712 4379
SexAI A'CCwGG_T
Cuts at: 3298 3298
Size: 6091
SfcI C'TryA_G
Cuts at: 826 1045 3439 4574 5255 5446 826
Size: 219 2394 1135 681 191 1471
Fragments arranged by size:
2394 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6091
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6091
SmaI CCC'GGG
Cuts at: 1872 3418 1872
Size: 1546 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4195 5096 5358 820
Size: 197 926 2252 901 262 1553
Fragments arranged by size:
2252 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3882 493
Size: 3389 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6091
SphI G_CATG'C
Cuts at: 1132 3449 1132
Size: 2317 3774
SspI AAT'ATT
Cuts at: 5 52 3057 4015 5
Size: 47 3005 958 2081
Fragments arranged by size:
3005 2081 958 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6091
StyI C'CwwG_G
Cuts at: 513 1065 2004 3104 3530 513
Size: 552 939 1100 426 3074
Fragments arranged by size:
3074 1100 939 552 426
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4118 4135 4288 4473 4918 325
Size: 1255 2538 17 153 185 445 1498
Fragments arranged by size:
2538 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2551 2706 4337
Size: 274 80 33 51 1443 574 155 1631
Cuts at: 4337 6049 96
Size: 1712 138
Fragments arranged by size:
1712 1631 1443 574 274 155 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6051 6083 6051
Size: 32 6059
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6091
XbaI T'CTAG_A
Cuts at: 3427 3427
Size: 6091
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6091
XmaI C'CCGG_G
Cuts at: 1870 3416 1870
Size: 1546 4545
XmnI GAAnn'nnTTC
Cuts at: 4220 4220
Size: 6091
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3899 276
Size: 53 83 186 936 2365 2468
Fragments arranged by size:
2468 2365 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PmlI PspXI RsrII SacII SfiI SfoI SrfI SwaI
XcmI