Restriction map for
pFN21AE0450
(Circular) (Six-base) MAPSORT of: pFN21AE0450.seq Check: 4832 from: 1 to: 6049
pFN21AE0450 6049 bp
With 182 enzymes: SgfI *
October 20, 2009 10:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2440 2708 2440
Size: 268 5781
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2760 3859 278
Size: 53 83 186 936 1224 1099 2468
Fragments arranged by size:
2468 1224 1099 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3392 1937
Size: 1455 4594
Acc65I G'GTAC_C
Cuts at: 3370 3370
Size: 6049
AclI AA'CG_TT
Cuts at: 1563 4176 4549 1563
Size: 2613 373 3063
Fragments arranged by size:
3063 2613 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2585 2719 2974 3158 4112 5496 5764 1632
Size: 953 134 255 184 954 1384 268 1917
Fragments arranged by size:
1917 1384 954 953 268 255 184 134
AflII C'TTAA_G
Cuts at: 820 1017 2080 820
Size: 197 1063 4789
Fragments arranged by size:
4789 1063 197
AflIII A'CryG_T
Cuts at: 1203 3131 4948 5925 1203
Size: 1928 1817 977 1327
Fragments arranged by size:
1928 1817 1327 977
AgeI A'CCGG_T
Cuts at: 4880 4880
Size: 6049
AhdI GACnn_n'nnGTC
Cuts at: 4778 4778
Size: 6049
AleI CACnn'nnGTG
Cuts at: 1166 2342 1166
Size: 1176 4873
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6049
AlwNI CAG_nnn'CTG
Cuts at: 2620 2968 3792 5364 2620
Size: 348 824 1572 3305
Fragments arranged by size:
3305 1572 824 348
ApaI G_GGCC'C
Cuts at: 2131 2131
Size: 6049
ApaLI G'TGCA_C
Cuts at: 4106 5262 4106
Size: 1156 4893
ApoI r'AATT_y
Cuts at: 1493 3358 3584 3736 5845 1493
Size: 1865 226 152 2109 1697
Fragments arranged by size:
2109 1865 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4603 160
Size: 872 3571 1606
Fragments arranged by size:
3571 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6049
AvaI C'yCGr_G
Cuts at: 1870 1943 2555 3374 1870
Size: 73 612 819 4545
Fragments arranged by size:
4545 819 612 73
BamHI G'GATC_C
Cuts at: 3379 3746 3379
Size: 367 5682
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2302 3091 3284 3370 4825
Size: 325 217 627 515 789 193 86 1455
Cuts at: 4825 618
Size: 1842
Fragments arranged by size:
1842 1455 789 627 515 325 217 193
86
BanII G_rGCy'C
Cuts at: 1391 2131 2574 2585 3368 1391
Size: 740 443 11 783 4072
Fragments arranged by size:
4072 783 740 443 11
BbeI G_GCGC'C
Cuts at: 2306 2306
Size: 6049
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2650 2814 3034 928
Size: 971 751 164 220 3943
Fragments arranged by size:
3943 971 751 220 164
BbvCI CC'TCA_GC
Cuts at: 2153 2436 2456 2153
Size: 283 20 5746
Fragments arranged by size:
5746 283 20
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4240 4274 1903
Size: 34 2303 34 3678
Fragments arranged by size:
3678 2303 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3941 5151 3941
Size: 1210 4839
BclI T'GATC_A
Cuts at: 1548 2934 1548
Size: 1386 4663
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2378 2468 2610
Size: 107 122 71 631 605 706 90 142
Cuts at: 2610 3257 4660 136
Size: 647 1403 1525
Fragments arranged by size:
1525 1403 706 647 631 605 142 122
107 90 71
BglII A'GATC_T
Cuts at: 6044 6044
Size: 6049
BlpI GC'TnA_GC
Cuts at: 2846 3278 3466 2846
Size: 432 188 5429
Fragments arranged by size:
5429 432 188
Bme1580I G_kGCm'C
Cuts at: 1790 2131 2874 3096 4110 5266 1790
Size: 341 743 222 1014 1156 2573
Fragments arranged by size:
2573 1156 1014 743 341 222
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6049
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2196 3335 3780
Size: 416 53 83 399 24 779 1139 445
Cuts at: 3780 4738 442
Size: 958 1753
Fragments arranged by size:
1753 1139 958 779 445 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6049
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2254 4709 1239
Size: 1015 2455 2579
Fragments arranged by size:
2579 2455 1015
Bpu10I CC'TnA_GC
Cuts at: 2153 2318 2436 2456 2153
Size: 165 118 20 5746
Fragments arranged by size:
5746 165 118 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2221 4174 5039 5337 2221
Size: 1953 865 298 2933
Fragments arranged by size:
2933 1953 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2431 4712 882
Size: 617 932 2281 2219
Fragments arranged by size:
2281 2219 932 617
BsaAI yAC'GTr
Cuts at: 493 1204 2869 3132 3840 493
Size: 711 1665 263 708 2702
Fragments arranged by size:
2702 1665 711 708 263
BsaBI GATnn'nnATC
Cuts at: 6043 6043
Size: 6049
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2303 2370 2757 3856
Size: 53 83 186 936 770 67 387 1099
Cuts at: 3856 4238 4873 275
Size: 382 635 1451
Fragments arranged by size:
1451 1099 936 770 635 387 382 186
83 67 53
BsaWI w'CCGG_w
Cuts at: 4482 4880 5154 5301 5712 4482
Size: 398 274 147 411 4819
Fragments arranged by size:
4819 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2219 2249 3236 3266 1178
Size: 30 165 30 816 30 987 30 3961
Fragments arranged by size:
3961 987 816 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2414 2993 1186
Size: 1228 579 4242
Fragments arranged by size:
4242 1228 579
BseYI C'CCAG_C
Cuts at: 2162 2586 2599 2961 3153 5252 5748 2162
Size: 424 13 362 192 2099 496 2463
Fragments arranged by size:
2463 2099 496 424 362 192 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3206 1163
Size: 573 1470 4006
Fragments arranged by size:
4006 1470 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3499 4260 5288 1540
Size: 275 186 1498 761 1028 2301
Fragments arranged by size:
2301 1498 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3003 4110 4195 5266 3003
Size: 1107 85 1071 3786
Fragments arranged by size:
3786 1107 1071 85
BsmI GAATG_Cn'
Cuts at: 3560 3653 3560
Size: 93 5956
BsmBI CGTCTCn'nnnn_
Cuts at: 2852 2852
Size: 6049
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2131 2574 2585 2874 3003 3096 3368
Size: 399 341 443 11 289 129 93 272
Cuts at: 3368 4110 4195 5266 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 443 399 341 289 272
129 93 85 11
BspEI T'CCGG_A
Cuts at: 5712 5712
Size: 6049
BspHI T'CATG_A
Cuts at: 3938 5668 3938
Size: 1730 4319
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2440 2508 2708 2965 3404 844
Size: 1596 68 200 257 439 3489
Fragments arranged by size:
3489 1596 439 257 200 68
BsrBI CCG'CTC
Cuts at: 1394 1823 2404 3282 3936 1394
Size: 429 581 878 654 3507
Fragments arranged by size:
3507 878 654 581 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2904 4544 4718 65
Size: 1183 1656 1640 174 1396
Fragments arranged by size:
1656 1640 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4693 4880 1700
Size: 110 2883 187 2869
Fragments arranged by size:
2883 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6049
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6049
BssSI C'ACGA_G
Cuts at: 2363 4109 5121 2363
Size: 1746 1012 3291
Fragments arranged by size:
3291 1746 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6049
BstBI TT'CG_AA
Cuts at: 4853 4853
Size: 6049
BstEII G'GTnAC_C
Cuts at: 1184 2323 1184
Size: 1139 4910
BstXI CCAn_nnnn'nTGG
Cuts at: 2599 2677 2887 2923 3076 2599
Size: 78 210 36 153 5572
Fragments arranged by size:
5572 210 153 78 36
BstYI r'GATC_y
Cuts at: 1972 2883 3379 3746 4131 4148 5600 5686 5698
Size: 911 496 367 385 17 1452 86 12
Cuts at: 5698 6044 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 911 496 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2241 2241
Size: 6049
BtgI C'CryG_G
Cuts at: 513 1065 2004 2120 2922 513
Size: 552 939 116 802 3640
Fragments arranged by size:
3640 939 802 552 116
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5310
Fragments arranged by size:
5310 683 56
BtsI GCAGTG_nn'
Cuts at: 2045 2157 3565 4359 4379 2045
Size: 112 1408 794 20 3715
Fragments arranged by size:
3715 1408 794 112 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6049
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2377 2412 529
Size: 35 1813 35 4166
Fragments arranged by size:
4166 1813 35 35
DraI TTT'AAA
Cuts at: 3353 3353
Size: 6049
DraIII CAC_nnn'GTG
Cuts at: 2176 2176
Size: 6049
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5056 809
Size: 507 3740 1802
Fragments arranged by size:
3740 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2649 2766 2826
Size: 54 1425 50 275 18 819 117 60
Cuts at: 2826 3207 3313 3496 4385 6014 8
Size: 381 106 183 889 1629 43
Fragments arranged by size:
1629 1425 889 819 381 275 183 117
106 60 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3496 1537
Size: 275 1684 4090
Fragments arranged by size:
4090 1684 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2738 3023 3979 1353
Size: 1385 285 956 3423
Fragments arranged by size:
3423 1385 956 285
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3175 3218 4636 5010 5156 387
Size: 1064 138 1586 43 1418 374 146 1280
Fragments arranged by size:
1586 1418 1280 1064 374 146 138 43
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2254 2585 2719 2974 3158 4112 4709
Size: 393 622 331 134 255 184 954 597
Cuts at: 4709 5496 5764 1239
Size: 787 268 1524
Fragments arranged by size:
1524 954 787 622 597 393 331 268
255 184 134
EcoO109I rG'GnC_Cy
Cuts at: 2075 2127 2380 2674 2892 3259 2075
Size: 52 253 294 218 367 4865
Fragments arranged by size:
4865 367 294 253 218 52
EcoRI G'AATT_C
Cuts at: 3358 3736 3358
Size: 378 5671
FspI TGC'GCA
Cuts at: 4555 4555
Size: 6049
HaeII r_GCGC'y
Cuts at: 1678 2306 2463 3278 5196 1678
Size: 628 157 815 1918 2531
Fragments arranged by size:
2531 1918 815 628 157
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3236 3268 4690 4722 4764
Size: 32 135 32 1664 32 1422 32 42
Cuts at: 4764 4796 1373
Size: 32 2626
Fragments arranged by size:
2626 1664 1422 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2524 3393 3639 669
Size: 988 281 586 869 246 3079
Fragments arranged by size:
3079 988 869 586 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6049
HpaI GTT'AAC
Cuts at: 3639 3639
Size: 6049
KasI G'GCGC_C
Cuts at: 2302 2302
Size: 6049
KpnI G_GTAC'C
Cuts at: 3374 3374
Size: 6049
MfeI C'AATT_G
Cuts at: 3648 3648
Size: 6049
MluI A'CGCG_T
Cuts at: 5925 5925
Size: 6049
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5163 5347 1305
Size: 3858 184 2007
Fragments arranged by size:
3858 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2651 2768 3209 3315 10
Size: 54 1425 343 819 117 441 106 2744
Fragments arranged by size:
2744 1425 819 441 343 117 106 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2261 2342 2774 2921 4007 4366 4525
Size: 648 1095 81 432 147 1086 359 159
Cuts at: 4525 518
Size: 2042
Fragments arranged by size:
2042 1095 1086 648 432 359 159 147
81
MspA1I CmG'CkG
Cuts at: 1606 2965 3157 3465 4142 5290 5535 1606
Size: 1359 192 308 677 1148 245 2120
Fragments arranged by size:
2120 1359 1148 677 308 245 192
NarI GG'CG_CC
Cuts at: 2303 2303
Size: 6049
NcoI C'CATG_G
Cuts at: 513 1065 2004 2120 513
Size: 552 939 116 4442
Fragments arranged by size:
4442 939 552 116
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6049
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6049
NotI GC'GGCC_GC
Cuts at: 3496 3496
Size: 6049
NspI r_CATG'y
Cuts at: 1132 1229 2089 3407 4952 1132
Size: 97 860 1318 1545 2229
Fragments arranged by size:
2229 1545 1318 860 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6049
PciI A'CATG_T
Cuts at: 4948 4948
Size: 6049
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2186 2620 1108
Size: 1078 434 4537
Fragments arranged by size:
4537 1078 434
PmeI GTTT'AAAC
Cuts at: 3353 3353
Size: 6049
PmlI CAC'GTG
Cuts at: 2869 3132 2869
Size: 263 5786
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4118 4150 5631 5663 4118
Size: 32 1481 32 4504
Fragments arranged by size:
4504 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2075 2892 2075
Size: 817 5232
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6049
PsiI TTA'TAA
Cuts at: 3619 3619
Size: 6049
PspOMI G'GGCC_C
Cuts at: 2127 2127
Size: 6049
PspXI vC'TCGA_Gb
Cuts at: 2555 2555
Size: 6049
PstI C_TGCA'G
Cuts at: 830 2976 3219 3401 830
Size: 2146 243 182 3478
Fragments arranged by size:
3478 2146 243 182
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6049
PvuII CAG'CTG
Cuts at: 2965 2965
Size: 6049
SalI G'TCGA_C
Cuts at: 1936 3391 1936
Size: 1455 4594
SanDI GG'GwC_CC
Cuts at: 2892 2892
Size: 6049
SbfI CC_TGCA'GG
Cuts at: 3401 3401
Size: 6049
ScaI AGT'ACT
Cuts at: 4297 6009 4297
Size: 1712 4337
SfcI C'TryA_G
Cuts at: 826 1045 2833 2972 3215 3325 3397 4532 5213
Size: 219 1788 139 243 110 72 1135 681
Cuts at: 5213 5404 826
Size: 191 1471
Fragments arranged by size:
1788 1471 1135 681 243 219 191 139
110 72
SfiI GGCCn_nnn'nGGCC
Cuts at: 2610 2610
Size: 6049
SfoI GGC'GCC
Cuts at: 2304 2304
Size: 6049
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6049
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6049
SmaI CCC'GGG
Cuts at: 1872 3376 1872
Size: 1504 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2080 2200 2555 4153 5054 5316
Size: 197 926 137 120 355 1598 901 262
Cuts at: 5316 820
Size: 1553
Fragments arranged by size:
1598 1553 926 901 355 262 197 137
120
SnaBI TAC'GTA
Cuts at: 493 3840 493
Size: 3347 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6049
SphI G_CATG'C
Cuts at: 1132 2089 3407 1132
Size: 957 1318 3774
Fragments arranged by size:
3774 1318 957
SspI AAT'ATT
Cuts at: 5 52 3973 5
Size: 47 3921 2081
Fragments arranged by size:
3921 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6049
StyI C'CwwG_G
Cuts at: 513 1065 2004 2120 2131 2652 2670 3204 3303
Size: 552 939 116 11 521 18 534 99
Cuts at: 3303 3488 513
Size: 185 3074
Fragments arranged by size:
3074 939 552 534 521 185 116 99
18 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2529 4076 4093 4246 4431 4876 325
Size: 1255 949 1547 17 153 185 445 1498
Fragments arranged by size:
1547 1498 1255 949 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4295 6007 96
Size: 274 80 33 51 1443 2318 1712 138
Fragments arranged by size:
2318 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6009 6041 6009
Size: 32 6017
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6049
XbaI T'CTAG_A
Cuts at: 3385 3385
Size: 6049
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3076 3076
Size: 6049
XhoI C'TCGA_G
Cuts at: 1943 2555 1943
Size: 612 5437
XmaI C'CCGG_G
Cuts at: 1870 3374 1870
Size: 1504 4545
XmnI GAAnn'nnTTC
Cuts at: 4178 4178
Size: 6049
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2758 3857 276
Size: 53 83 186 936 1224 1099 2468
Fragments arranged by size:
2468 1224 1099 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SalI SanDI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BstZ17I EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PfoI PsrI RsrII SacI
SacII SapI SexAI SrfI SwaI