Restriction map for
pFN21AE0449
(Circular) (Six-base) MAPSORT of: pFN21AE0449.seq Check: 4383 from: 1 to: 5518
pFN21AE0449 5518 bp
With 182 enzymes: SgfI *
October 20, 2009 10:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3328 278
Size: 53 83 186 936 1792 2468
Fragments arranged by size:
2468 1792 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2287 2861 1937
Size: 350 574 4594
Fragments arranged by size:
4594 574 350
Acc65I G'GTAC_C
Cuts at: 2839 2839
Size: 5518
AclI AA'CG_TT
Cuts at: 1563 3645 4018 1563
Size: 2082 373 3063
Fragments arranged by size:
3063 2082 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2046 2205 2474 2539 3581 4965 5233 1632
Size: 414 159 269 65 1042 1384 268 1917
Fragments arranged by size:
1917 1384 1042 414 269 268 159 65
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5321
AflIII A'CryG_T
Cuts at: 1203 4417 5394 1203
Size: 3214 977 1327
Fragments arranged by size:
3214 1327 977
AgeI A'CCGG_T
Cuts at: 4349 4349
Size: 5518
AhdI GACnn_n'nnGTC
Cuts at: 2294 4247 2294
Size: 1953 3565
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5518
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5518
AlwNI CAG_nnn'CTG
Cuts at: 2249 2712 3261 4833 2249
Size: 463 549 1572 2934
Fragments arranged by size:
2934 1572 549 463
ApaLI G'TGCA_C
Cuts at: 3575 4731 3575
Size: 1156 4362
ApoI r'AATT_y
Cuts at: 1493 2827 3053 3205 5314 1493
Size: 1334 226 152 2109 1697
Fragments arranged by size:
2109 1697 1334 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4072 160
Size: 872 3040 1606
Fragments arranged by size:
3040 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5518
AvaI C'yCGr_G
Cuts at: 1870 1943 2071 2632 2843 1870
Size: 73 128 561 211 4545
Fragments arranged by size:
4545 561 211 128 73
BamHI G'GATC_C
Cuts at: 2848 3215 2848
Size: 367 5151
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2839 4294 618
Size: 325 217 627 1052 1455 1842
Fragments arranged by size:
1842 1455 1052 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2197 2837 1391
Size: 806 640 4072
Fragments arranged by size:
4072 806 640
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4547
BbvCI CC'TCA_GC
Cuts at: 2753 2753
Size: 5518
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3709 3743 1903
Size: 34 50 34 1688 34 3678
Fragments arranged by size:
3678 1688 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3410 4620 3410
Size: 1210 4308
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5518
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4129 136
Size: 107 122 71 631 605 2457 1525
Fragments arranged by size:
2457 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2808 5513 2808
Size: 2705 2813
BlpI GC'TnA_GC
Cuts at: 2935 2935
Size: 5518
Bme1580I G_kGCm'C
Cuts at: 1790 3579 4735 1790
Size: 1789 1156 2573
Fragments arranged by size:
2573 1789 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5518
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3249 4207 442
Size: 416 53 83 399 24 1832 958 1753
Fragments arranged by size:
1832 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5518
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2504 2702 4178 1239
Size: 1265 198 1476 2579
Fragments arranged by size:
2579 1476 1265 198
Bpu10I CC'TnA_GC
Cuts at: 2753 2753
Size: 5518
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3643 4508 4806 3643
Size: 865 298 4355
Fragments arranged by size:
4355 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2352 4181 882
Size: 617 853 1829 2219
Fragments arranged by size:
2219 1829 853 617
BsaAI yAC'GTr
Cuts at: 493 1204 3309 493
Size: 711 2105 2702
Fragments arranged by size:
2702 2105 711
BsaBI GATnn'nnATC
Cuts at: 5512 5512
Size: 5518
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2055 2290 3325 3707
Size: 53 83 186 936 522 235 1035 382
Cuts at: 3707 4342 275
Size: 635 1451
Fragments arranged by size:
1451 1035 936 635 522 382 235 186
83 53
BsaWI w'CCGG_w
Cuts at: 3951 4349 4623 4770 5181 3951
Size: 398 274 147 411 4288
Fragments arranged by size:
4288 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5293
Fragments arranged by size:
5293 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2552 1186
Size: 1366 4152
BseYI C'CCAG_C
Cuts at: 2402 4721 5217 2402
Size: 2319 496 2703
Fragments arranged by size:
2703 2319 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2023 2632 2645 1163
Size: 573 287 609 13 4036
Fragments arranged by size:
4036 609 573 287 13
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2968 3729 4757 1540
Size: 275 186 967 761 1028 2301
Fragments arranged by size:
2301 1028 967 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2197 2597 3579 3664 4735 2197
Size: 400 982 85 1071 2980
Fragments arranged by size:
2980 1071 982 400 85
BsmI GAATG_Cn'
Cuts at: 3029 3122 3029
Size: 93 5425
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2197 2597 2837 3579 3664 4735 1391
Size: 399 407 400 240 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 407 400 399 240 85
BspEI T'CCGG_A
Cuts at: 5181 5181
Size: 5518
BspHI T'CATG_A
Cuts at: 3407 5137 3407
Size: 1730 3788
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2574 2873 844
Size: 1730 299 3489
Fragments arranged by size:
3489 1730 299
BsrBI CCG'CTC
Cuts at: 1394 1823 2589 3405 1394
Size: 429 766 816 3507
Fragments arranged by size:
3507 816 766 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4013 4187 65
Size: 1183 2765 174 1396
Fragments arranged by size:
2765 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4162 4349 1700
Size: 110 2352 187 2869
Fragments arranged by size:
2869 2352 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5518
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5518
BssSI C'ACGA_G
Cuts at: 3578 4590 3578
Size: 1012 4506
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2712 794
Size: 1918 3600
BstBI TT'CG_AA
Cuts at: 4322 4322
Size: 5518
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5518
BstXI CCAn_nnnn'nTGG
Cuts at: 2402 2795 2402
Size: 393 5125
BstYI r'GATC_y
Cuts at: 1972 2808 2848 3215 3600 3617 5069 5155 5167
Size: 836 40 367 385 17 1452 86 12
Cuts at: 5167 5513 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 836 385 367 346 86 40
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2617 513
Size: 552 1552 3414
Fragments arranged by size:
3414 1552 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4779
Fragments arranged by size:
4779 683 56
BtsI GCAGTG_nn'
Cuts at: 2746 3034 3828 3848 2746
Size: 288 794 20 4416
Fragments arranged by size:
4416 794 288 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5518
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2132 2167 529
Size: 35 1568 35 3880
Fragments arranged by size:
3880 1568 35 35
DraI TTT'AAA
Cuts at: 2822 2822
Size: 5518
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4525 809
Size: 507 3209 1802
Fragments arranged by size:
3209 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2965 3854 5483
Size: 54 1425 50 275 18 1135 889 1629
Cuts at: 5483 8
Size: 43
Fragments arranged by size:
1629 1425 1135 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2965 1537
Size: 275 1153 4090
Fragments arranged by size:
4090 1153 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2106 3448 1353
Size: 753 1342 3423
Fragments arranged by size:
3423 1342 753
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4105 4479 4625 387
Size: 1064 138 2516 374 146 1280
Fragments arranged by size:
2516 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2195 2195
Size: 5518
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2046 2205 2474 2504 2539 2702 3581
Size: 393 414 159 269 30 35 163 879
Cuts at: 3581 4178 4965 5233 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 879 787 597 414 393 269 268
163 159 35 30
EcoNI CCTnn'n_nnAGG
Cuts at: 2103 2103
Size: 5518
EcoO109I rG'GnC_Cy
Cuts at: 2546 2657 2759 2546
Size: 111 102 5305
Fragments arranged by size:
5305 111 102
EcoRI G'AATT_C
Cuts at: 2827 3205 2827
Size: 378 5140
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2183 2183
Size: 5518
FspI TGC'GCA
Cuts at: 4024 4024
Size: 5518
HaeII r_GCGC'y
Cuts at: 1678 4665 1678
Size: 2987 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2148 2180 2280 2312 4159
Size: 32 135 32 576 32 100 32 1847
Cuts at: 4159 4191 4233 4265 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1847 576 135 100 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2862 3108 669
Size: 988 281 924 246 3079
Fragments arranged by size:
3079 988 924 281 246
HindIII A'AGCT_T
Cuts at: 748 2146 748
Size: 1398 4120
HpaI GTT'AAC
Cuts at: 3108 3108
Size: 5518
KpnI G_GTAC'C
Cuts at: 2843 2843
Size: 5518
MfeI C'AATT_G
Cuts at: 3117 3117
Size: 5518
MluI A'CGCG_T
Cuts at: 5394 5394
Size: 5518
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4632 4816 1305
Size: 3327 184 2007
Fragments arranged by size:
3327 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3696
Fragments arranged by size:
3696 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3476 3835 3994 518
Size: 648 2310 359 159 2042
Fragments arranged by size:
2310 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2092 2934 3611 4759 5004 1606
Size: 486 842 677 1148 245 2120
Fragments arranged by size:
2120 1148 842 677 486 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4966
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5518
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5518
NotI GC'GGCC_GC
Cuts at: 2965 2965
Size: 5518
NspI r_CATG'y
Cuts at: 1132 1229 2143 2876 4421 1132
Size: 97 914 733 1545 2229
Fragments arranged by size:
2229 1545 914 733 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5518
PciI A'CATG_T
Cuts at: 4417 4417
Size: 5518
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2482 1108
Size: 1374 4144
PfoI T'CCnGG_A
Cuts at: 2718 2718
Size: 5518
PmeI GTTT'AAAC
Cuts at: 2822 2822
Size: 5518
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3587 3619 5100 5132 3587
Size: 32 1481 32 3973
Fragments arranged by size:
3973 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2657 2759 2657
Size: 102 5416
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5518
PsiI TTA'TAA
Cuts at: 3088 3088
Size: 5518
PstI C_TGCA'G
Cuts at: 830 2091 2734 2741 2870 830
Size: 1261 643 7 129 3478
Fragments arranged by size:
3478 1261 643 129 7
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5518
PvuII CAG'CTG
Cuts at: 2092 2092
Size: 5518
SacI G_AGCT'C
Cuts at: 2197 2197
Size: 5518
SalI G'TCGA_C
Cuts at: 1936 2860 1936
Size: 924 4594
SanDI GG'GwC_CC
Cuts at: 2657 2657
Size: 5518
SbfI CC_TGCA'GG
Cuts at: 2870 2870
Size: 5518
ScaI AGT'ACT
Cuts at: 3766 5478 3766
Size: 1712 3806
SexAI A'CCwGG_T
Cuts at: 2113 2113
Size: 5518
SfcI C'TryA_G
Cuts at: 826 1045 2087 2315 2730 2737 2866 4001 4682
Size: 219 1042 228 415 7 129 1135 681
Cuts at: 4682 4873 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1042 681 415 228 219 191
129 7
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5518
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5518
SmaI CCC'GGG
Cuts at: 1872 2845 1872
Size: 973 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3622 4523 4785 820
Size: 197 926 1679 901 262 1553
Fragments arranged by size:
1679 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3309 493
Size: 2816 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5518
SphI G_CATG'C
Cuts at: 1132 2876 1132
Size: 1744 3774
SspI AAT'ATT
Cuts at: 5 52 3442 5
Size: 47 3390 2081
Fragments arranged by size:
3390 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5518
StyI C'CwwG_G
Cuts at: 513 1065 2957 513
Size: 552 1892 3074
Fragments arranged by size:
3074 1892 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3545 3562 3715 3900 4345 325
Size: 1255 1965 17 153 185 445 1498
Fragments arranged by size:
1965 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3764 5476 96
Size: 274 80 33 51 1443 1787 1712 138
Fragments arranged by size:
1787 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2487 2519 5478 5510 2487
Size: 32 2959 32 2495
Fragments arranged by size:
2959 2495 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5518
XbaI T'CTAG_A
Cuts at: 2350 2461 2854 2350
Size: 111 393 5014
Fragments arranged by size:
5014 393 111
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5518
XmaI C'CCGG_G
Cuts at: 1870 2843 1870
Size: 973 4545
XmnI GAAnn'nnTTC
Cuts at: 3647 3647
Size: 5518
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3326 276
Size: 53 83 186 936 1792 2468
Fragments arranged by size:
2468 1792 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SanDI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacII SapI
SfiI SfoI SrfI SwaI XcmI