Restriction map for
pFN21AE0445
(Circular) (Six-base) MAPSORT of: pFN21AE0445.seq Check: 3004 from: 1 to: 5665
pFN21AE0445 5665 bp
With 182 enzymes: SgfI *
October 20, 2009 10:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3475 278
Size: 53 83 186 936 1939 2468
Fragments arranged by size:
2468 1939 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2739 3008 1937
Size: 802 269 4594
Fragments arranged by size:
4594 802 269
Acc65I G'GTAC_C
Cuts at: 2986 2986
Size: 5665
AclI AA'CG_TT
Cuts at: 1563 3792 4165 1563
Size: 2229 373 3063
Fragments arranged by size:
3063 2229 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2073 3728 5112 5380 1632
Size: 441 1655 1384 268 1917
Fragments arranged by size:
1917 1655 1384 441 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5468
AflIII A'CryG_T
Cuts at: 1203 4564 5541 1203
Size: 3361 977 1327
Fragments arranged by size:
3361 1327 977
AgeI A'CCGG_T
Cuts at: 4496 4496
Size: 5665
AhdI GACnn_n'nnGTC
Cuts at: 4394 4394
Size: 5665
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5665
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5665
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2065 2097 2065
Size: 32 5633
AlwNI CAG_nnn'CTG
Cuts at: 2168 3408 4980 2168
Size: 1240 1572 2853
Fragments arranged by size:
2853 1572 1240
ApaLI G'TGCA_C
Cuts at: 2559 2756 3722 4878 2559
Size: 197 966 1156 3346
Fragments arranged by size:
3346 1156 966 197
ApoI r'AATT_y
Cuts at: 1493 2974 3200 3352 5461 1493
Size: 1481 226 152 2109 1697
Fragments arranged by size:
2109 1697 1481 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4219 160
Size: 872 3187 1606
Fragments arranged by size:
3187 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5665
AvaI C'yCGr_G
Cuts at: 1870 1943 2990 1870
Size: 73 1047 4545
Fragments arranged by size:
4545 1047 73
BamHI G'GATC_C
Cuts at: 2995 3362 2995
Size: 367 5298
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2265 2285 2986 4441 618
Size: 325 217 627 478 20 701 1455 1842
Fragments arranged by size:
1842 1455 701 627 478 325 217 20
BanII G_rGCy'C
Cuts at: 1391 2984 1391
Size: 1593 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2958 928
Size: 971 1059 3635
Fragments arranged by size:
3635 1059 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3856 3890 1903
Size: 34 1919 34 3678
Fragments arranged by size:
3678 1919 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2370 3557 4767 2370
Size: 1187 1210 3268
Fragments arranged by size:
3268 1210 1187
BclI T'GATC_A
Cuts at: 1548 2391 1548
Size: 843 4822
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2022 4276 136
Size: 107 122 71 631 605 350 2254 1525
Fragments arranged by size:
2254 1525 631 605 350 122 107 71
BglII A'GATC_T
Cuts at: 2527 5660 2527
Size: 3133 2532
BlpI GC'TnA_GC
Cuts at: 3082 3082
Size: 5665
Bme1580I G_kGCm'C
Cuts at: 1790 2136 2290 2563 2760 3726 4882 1790
Size: 346 154 273 197 966 1156 2573
Fragments arranged by size:
2573 1156 966 346 273 197 154
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5665
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3396 4354 442
Size: 416 53 83 399 24 1979 958 1753
Fragments arranged by size:
1979 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5665
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2544 2901 4325 1239
Size: 1305 357 1424 2579
Fragments arranged by size:
2579 1424 1305 357
Bpu10I CC'TnA_GC
Cuts at: 2870 2870
Size: 5665
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3790 4655 4953 3790
Size: 865 298 4502
Fragments arranged by size:
4502 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2781 4328 882
Size: 617 1282 1547 2219
Fragments arranged by size:
2219 1547 1282 617
BsaAI yAC'GTr
Cuts at: 493 1204 3456 493
Size: 711 2252 2702
Fragments arranged by size:
2702 2252 711
BsaBI GATnn'nnATC
Cuts at: 2393 5659 2393
Size: 3266 2399
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3472 3854 4489 275
Size: 53 83 186 936 1939 382 635 1451
Fragments arranged by size:
1939 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4098 4496 4770 4917 5328 4098
Size: 398 274 147 411 4435
Fragments arranged by size:
4435 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2009 2039 1178
Size: 30 165 30 606 30 4804
Fragments arranged by size:
4804 606 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5665
BseYI C'CCAG_C
Cuts at: 2141 4868 5364 2141
Size: 2727 496 2442
Fragments arranged by size:
2727 2442 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2593 2750 1163
Size: 573 857 157 4078
Fragments arranged by size:
4078 857 573 157
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3115 3876 4904 1540
Size: 275 186 1114 761 1028 2301
Fragments arranged by size:
2301 1114 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2563 2760 3726 3811 4882 2563
Size: 197 966 85 1071 3346
Fragments arranged by size:
3346 1071 966 197 85
BsmI GAATG_Cn'
Cuts at: 3176 3269 3176
Size: 93 5572
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2136 2290 2563 2760 2984 3726 3811
Size: 399 346 154 273 197 224 742 85
Cuts at: 3811 4882 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 346 273 224 197
154 85
BspEI T'CCGG_A
Cuts at: 5328 5328
Size: 5665
BspHI T'CATG_A
Cuts at: 2109 3554 5284 2109
Size: 1445 1730 2490
Fragments arranged by size:
2490 1730 1445
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3020 844
Size: 2176 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3552 1394
Size: 429 1729 3507
Fragments arranged by size:
3507 1729 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2136 4160 4334 65
Size: 1183 888 2024 174 1396
Fragments arranged by size:
2024 1396 1183 888 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4309 4496 1700
Size: 110 2499 187 2869
Fragments arranged by size:
2869 2499 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5665
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5665
BssSI C'ACGA_G
Cuts at: 3725 4737 3725
Size: 1012 4653
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2764 794
Size: 1970 3695
BstBI TT'CG_AA
Cuts at: 4469 4469
Size: 5665
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5665
BstXI CCAn_nnnn'nTGG
Cuts at: 2908 2908
Size: 5665
BstYI r'GATC_y
Cuts at: 1972 2277 2373 2527 2995 3362 3747 3764 5216
Size: 305 96 154 468 367 385 17 1452
Cuts at: 5216 5302 5314 5660 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 468 385 367 346 305 154
96 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5113
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4926
Fragments arranged by size:
4926 683 56
BtsI GCAGTG_nn'
Cuts at: 2011 2418 2758 3181 3975 3995 2011
Size: 407 340 423 794 20 3681
Fragments arranged by size:
3681 794 423 407 340 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5665
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5630
DraI TTT'AAA
Cuts at: 2969 2969
Size: 5665
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4672 809
Size: 507 3356 1802
Fragments arranged by size:
3356 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2418 2461 2637
Size: 54 1425 50 275 18 588 43 176
Cuts at: 2637 3112 4001 5630 8
Size: 475 889 1629 43
Fragments arranged by size:
1629 1425 889 588 475 275 176 54
50 43 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3112 1537
Size: 275 1300 4090
Fragments arranged by size:
4090 1300 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2216 3595 1353
Size: 863 1379 3423
Fragments arranged by size:
3423 1379 863
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4252 4626 4772 387
Size: 1064 138 2663 374 146 1280
Fragments arranged by size:
2663 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2073 2544 2901 3728 4325 5112 5380
Size: 393 441 471 357 827 597 787 268
Cuts at: 5380 1239
Size: 1524
Fragments arranged by size:
1524 827 787 597 471 441 393 357
268
EcoO109I rG'GnC_Cy
Cuts at: 2929 2929
Size: 5665
EcoRI G'AATT_C
Cuts at: 2974 3352 2974
Size: 378 5287
FspI TGC'GCA
Cuts at: 2410 4171 2410
Size: 1761 3904
HaeII r_GCGC'y
Cuts at: 1678 2353 4812 1678
Size: 675 2459 2531
Fragments arranged by size:
2531 2459 675
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2262 2294 2899 2931 4306
Size: 32 135 32 690 32 605 32 1375
Cuts at: 4306 4338 4380 4412 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1375 690 605 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2740 2923 3009 3255 669
Size: 988 281 802 183 86 246 3079
Fragments arranged by size:
3079 988 802 281 246 183 86
HindIII A'AGCT_T
Cuts at: 748 2878 748
Size: 2130 3535
HpaI GTT'AAC
Cuts at: 3255 3255
Size: 5665
KpnI G_GTAC'C
Cuts at: 2990 2990
Size: 5665
MfeI C'AATT_G
Cuts at: 3264 3264
Size: 5665
MluI A'CGCG_T
Cuts at: 5541 5541
Size: 5665
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4779 4963 1305
Size: 3474 184 2007
Fragments arranged by size:
3474 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2463 2639 10
Size: 54 1425 343 631 176 3036
Fragments arranged by size:
3036 1425 631 343 176 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2108 2260 2312 2906 3623 3982 4141
Size: 648 942 152 52 594 717 359 159
Cuts at: 4141 518
Size: 2042
Fragments arranged by size:
2042 942 717 648 594 359 159 152
52
MspA1I CmG'CkG
Cuts at: 1606 2406 3081 3758 4906 5151 1606
Size: 800 675 677 1148 245 2120
Fragments arranged by size:
2120 1148 800 677 675 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5113
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5665
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5665
NotI GC'GGCC_GC
Cuts at: 3112 3112
Size: 5665
NspI r_CATG'y
Cuts at: 1132 1229 2346 2519 3023 4568 1132
Size: 97 1117 173 504 1545 2229
Fragments arranged by size:
2229 1545 1117 504 173 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5665
PciI A'CATG_T
Cuts at: 4564 4564
Size: 5665
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5665
PmeI GTTT'AAAC
Cuts at: 2969 2969
Size: 5665
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3734 3766 5247 5279 3734
Size: 32 1481 32 4120
Fragments arranged by size:
4120 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2929 2929
Size: 5665
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5665
PsiI TTA'TAA
Cuts at: 3235 3235
Size: 5665
PstI C_TGCA'G
Cuts at: 830 3017 830
Size: 2187 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5665
PvuII CAG'CTG
Cuts at: 2406 2406
Size: 5665
SalI G'TCGA_C
Cuts at: 1936 2738 3007 1936
Size: 802 269 4594
Fragments arranged by size:
4594 802 269
SbfI CC_TGCA'GG
Cuts at: 3017 3017
Size: 5665
ScaI AGT'ACT
Cuts at: 3913 5625 3913
Size: 1712 3953
SfcI C'TryA_G
Cuts at: 826 1045 2817 3013 4148 4829 5020 826
Size: 219 1772 196 1135 681 191 1471
Fragments arranged by size:
1772 1471 1135 681 219 196 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5665
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5665
SmaI CCC'GGG
Cuts at: 1872 2992 1872
Size: 1120 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3769 4670 4932 820
Size: 197 926 1826 901 262 1553
Fragments arranged by size:
1826 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3456 493
Size: 2963 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5665
SphI G_CATG'C
Cuts at: 1132 2519 3023 1132
Size: 1387 504 3774
Fragments arranged by size:
3774 1387 504
SspI AAT'ATT
Cuts at: 5 52 3589 5
Size: 47 3537 2081
Fragments arranged by size:
3537 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5665
StyI C'CwwG_G
Cuts at: 513 1065 2281 2458 3104 513
Size: 552 1216 177 646 3074
Fragments arranged by size:
3074 1216 646 552 177
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2117 2302 3692 3709 3862 4047 4492
Size: 1255 537 185 1390 17 153 185 445
Cuts at: 4492 325
Size: 1498
Fragments arranged by size:
1498 1390 1255 537 445 185 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2428 2479 3911
Size: 274 80 33 51 1443 451 51 1432
Cuts at: 3911 5623 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1432 451 274 138 80 51
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2009 2041 5625 5657 2009
Size: 32 3584 32 2017
Fragments arranged by size:
3584 2017 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5665
XbaI T'CTAG_A
Cuts at: 3001 3001
Size: 5665
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2018 2410 2456 2632 2677 2018
Size: 392 46 176 45 5006
Fragments arranged by size:
5006 392 176 46 45
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5665
XmaI C'CCGG_G
Cuts at: 1870 2990 1870
Size: 1120 4545
XmnI GAAnn'nnTTC
Cuts at: 3794 3794
Size: 5665
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3473 276
Size: 53 83 186 936 1939 2468
Fragments arranged by size:
2468 1939 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI