Restriction map for
pFN21AE0443
(Circular) (Six-base) MAPSORT of: pFN21AE0443.seq Check: 2094 from: 1 to: 5671
pFN21AE0443 5671 bp
With 182 enzymes: SgfI *
October 20, 2009 10:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3481 278
Size: 53 83 186 936 1945 2468
Fragments arranged by size:
2468 1945 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3014 1937
Size: 1077 4594
Acc65I G'GTAC_C
Cuts at: 2992 2992
Size: 5671
AclI AA'CG_TT
Cuts at: 1563 3798 4171 1563
Size: 2235 373 3063
Fragments arranged by size:
3063 2235 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3734 5118 5386 1632
Size: 2102 1384 268 1917
Fragments arranged by size:
2102 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5474
AflIII A'CryG_T
Cuts at: 1203 4570 5547 1203
Size: 3367 977 1327
Fragments arranged by size:
3367 1327 977
AgeI A'CCGG_T
Cuts at: 4502 4502
Size: 5671
AhdI GACnn_n'nnGTC
Cuts at: 4400 4400
Size: 5671
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5671
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5671
AlwNI CAG_nnn'CTG
Cuts at: 2062 2776 2810 3414 4986 2062
Size: 714 34 604 1572 2747
Fragments arranged by size:
2747 1572 714 604 34
ApaI G_GGCC'C
Cuts at: 2445 2445
Size: 5671
ApaLI G'TGCA_C
Cuts at: 2102 3728 4884 2102
Size: 1626 1156 2889
Fragments arranged by size:
2889 1626 1156
ApoI r'AATT_y
Cuts at: 1493 2980 3206 3358 5467 1493
Size: 1487 226 152 2109 1697
Fragments arranged by size:
2109 1697 1487 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4225 160
Size: 872 3193 1606
Fragments arranged by size:
3193 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5671
AvaI C'yCGr_G
Cuts at: 1870 1943 2328 2462 2996 1870
Size: 73 385 134 534 4545
Fragments arranged by size:
4545 534 385 134 73
BamHI G'GATC_C
Cuts at: 3001 3368 3001
Size: 367 5304
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2144 2201 2645 2992 4447
Size: 325 217 627 357 57 444 347 1455
Cuts at: 4447 618
Size: 1842
Fragments arranged by size:
1842 1455 627 444 357 347 325 217
57
BanII G_rGCy'C
Cuts at: 1391 2039 2099 2445 2491 2910 2990 1391
Size: 648 60 346 46 419 80 4072
Fragments arranged by size:
4072 648 419 346 80 60 46
BbeI G_GCGC'C
Cuts at: 2148 2205 2148
Size: 57 5614
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4700
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3862 3896 1903
Size: 34 1925 34 3678
Fragments arranged by size:
3678 1925 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3563 4773 3563
Size: 1210 4461
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5671
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2117 2333 4282
Size: 107 122 71 631 605 445 216 1949
Cuts at: 4282 136
Size: 1525
Fragments arranged by size:
1949 1525 631 605 445 216 122 107
71
BglII A'GATC_T
Cuts at: 5666 5666
Size: 5671
BlpI GC'TnA_GC
Cuts at: 2252 3088 2252
Size: 836 4835
Bme1580I G_kGCm'C
Cuts at: 1790 2106 2445 2648 3732 4888 1790
Size: 316 339 203 1084 1156 2573
Fragments arranged by size:
2573 1156 1084 339 316 203
BmgBI CAC'GTC
Cuts at: 1318 2378 1318
Size: 1060 4611
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2894 3402 4360
Size: 416 53 83 399 24 1477 508 958
Cuts at: 4360 442
Size: 1753
Fragments arranged by size:
1753 1477 958 508 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5671
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2033 2750 4331 1239
Size: 794 717 1581 2579
Fragments arranged by size:
2579 1581 794 717
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3796 4661 4959 3796
Size: 865 298 4508
Fragments arranged by size:
4508 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2459 2726 4334 882
Size: 617 960 267 1608 2219
Fragments arranged by size:
2219 1608 960 617 267
BsaAI yAC'GTr
Cuts at: 493 1204 2695 3462 493
Size: 711 1491 767 2702
Fragments arranged by size:
2702 1491 767 711
BsaBI GATnn'nnATC
Cuts at: 5665 5665
Size: 5671
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2145 2202 3478 3860
Size: 53 83 186 936 612 57 1276 382
Cuts at: 3860 4495 275
Size: 635 1451
Fragments arranged by size:
1451 1276 936 635 612 382 186 83
57 53
BsaWI w'CCGG_w
Cuts at: 2796 2910 4104 4502 4776 4923 5334 2796
Size: 114 1194 398 274 147 411 3133
Fragments arranged by size:
3133 1194 411 398 274 147 114
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5446
Fragments arranged by size:
5446 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2918 1186
Size: 1732 3939
BseYI C'CCAG_C
Cuts at: 2705 4874 5370 2705
Size: 2169 496 3006
Fragments arranged by size:
3006 2169 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5098
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2765 3121 3882 4910 1540
Size: 275 186 764 356 761 1028 2301
Fragments arranged by size:
2301 1028 764 761 356 275 186
BsiHKAI G_wGCw'C
Cuts at: 2106 2463 2910 3732 3817 4888 2106
Size: 357 447 822 85 1071 2889
Fragments arranged by size:
2889 1071 822 447 357 85
BsmI GAATG_Cn'
Cuts at: 2682 3182 3275 2682
Size: 500 93 5078
Fragments arranged by size:
5078 500 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2368 2368
Size: 5671
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2039 2099 2106 2445 2463 2491 2648
Size: 399 249 60 7 339 18 28 157
Cuts at: 2648 2910 2990 3732 3817 4888 1391
Size: 262 80 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 339 262 249 157
85 80 60 28 18 7
BspEI T'CCGG_A
Cuts at: 2796 2910 5334 2796
Size: 114 2424 3133
Fragments arranged by size:
3133 2424 114
BspHI T'CATG_A
Cuts at: 3560 5290 3560
Size: 1730 3941
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3026 844
Size: 2182 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3558 1394
Size: 429 1735 3507
Fragments arranged by size:
3507 1735 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4166 4340 65
Size: 1183 2918 174 1396
Fragments arranged by size:
2918 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2735 4315 4502 1700
Size: 110 925 1580 187 2869
Fragments arranged by size:
2869 1580 925 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5671
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5671
BssSI C'ACGA_G
Cuts at: 2228 3731 4743 2228
Size: 1503 1012 3156
Fragments arranged by size:
3156 1503 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5671
BstBI TT'CG_AA
Cuts at: 4475 4475
Size: 5671
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5671
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2161 2641 2011
Size: 150 480 5041
Fragments arranged by size:
5041 480 150
BstYI r'GATC_y
Cuts at: 1972 2046 2523 3001 3368 3753 3770 5222 5308
Size: 74 477 478 367 385 17 1452 86
Cuts at: 5308 5320 5666 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 478 477 385 367 346 86
74 17 12
Bsu36I CC'TnA_GG
Cuts at: 2901 2901
Size: 5671
BtgI C'CryG_G
Cuts at: 513 1065 2184 2565 2677 513
Size: 552 1119 381 112 3507
Fragments arranged by size:
3507 1119 552 381 112
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4932
Fragments arranged by size:
4932 683 56
BtsI GCAGTG_nn'
Cuts at: 2432 3187 3981 4001 2432
Size: 755 794 20 4102
Fragments arranged by size:
4102 794 755 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5671
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5636
DraI TTT'AAA
Cuts at: 2975 2975
Size: 5671
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4678 809
Size: 507 3362 1802
Fragments arranged by size:
3362 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2151 2658 3118
Size: 54 1425 50 275 18 321 507 460
Cuts at: 3118 4007 5636 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 507 460 321 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3118 1537
Size: 275 1306 4090
Fragments arranged by size:
4090 1306 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2352 2400 2716 2751 2941 3601 1353
Size: 999 48 316 35 190 660 3423
Fragments arranged by size:
3423 999 660 316 190 48 35
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4258 4632 4778 387
Size: 1064 138 2669 374 146 1280
Fragments arranged by size:
2669 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2908 2908
Size: 5671
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2033 2750 3734 4331 5118 5386 1239
Size: 393 401 717 984 597 787 268 1524
Fragments arranged by size:
1524 984 787 717 597 401 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2441 2442 2441
Size: 1 5670
EcoRI G'AATT_C
Cuts at: 2980 3358 2980
Size: 378 5293
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2272 2272
Size: 5671
FspI TGC'GCA
Cuts at: 4177 4177
Size: 5671
HaeII r_GCGC'y
Cuts at: 1678 2148 2205 2352 4818 1678
Size: 470 57 147 2466 2531
Fragments arranged by size:
2531 2466 470 147 57
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2202 2234 4312 4344 4386
Size: 32 135 32 630 32 2078 32 42
Cuts at: 4386 4418 1373
Size: 32 2626
Fragments arranged by size:
2626 2078 630 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2287 3015 3261 669
Size: 988 281 349 728 246 3079
Fragments arranged by size:
3079 988 728 349 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5671
HpaI GTT'AAC
Cuts at: 3261 3261
Size: 5671
KasI G'GCGC_C
Cuts at: 2144 2201 2144
Size: 57 5614
KpnI G_GTAC'C
Cuts at: 2996 2996
Size: 5671
MfeI C'AATT_G
Cuts at: 3270 3270
Size: 5671
MluI A'CGCG_T
Cuts at: 5547 5547
Size: 5671
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4785 4969 1305
Size: 3480 184 2007
Fragments arranged by size:
3480 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2660 10
Size: 54 1425 343 828 3021
Fragments arranged by size:
3021 1425 828 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2135 3629 3988 4147 518
Size: 648 969 1494 359 159 2042
Fragments arranged by size:
2042 1494 969 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2059 2282 2588 2656 3087 3764 4912 5157
Size: 453 223 306 68 431 677 1148 245
Cuts at: 5157 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 453 431 306 245 223
68
NarI GG'CG_CC
Cuts at: 2145 2202 2145
Size: 57 5614
NcoI C'CATG_G
Cuts at: 513 1065 2565 2677 513
Size: 552 1500 112 3507
Fragments arranged by size:
3507 1500 552 112
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5671
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5671
NotI GC'GGCC_GC
Cuts at: 3118 3118
Size: 5671
NspI r_CATG'y
Cuts at: 1132 1229 3029 4574 1132
Size: 97 1800 1545 2229
Fragments arranged by size:
2229 1800 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2446 1348
Size: 1098 4573
PciI A'CATG_T
Cuts at: 4570 4570
Size: 5671
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5671
PfoI T'CCnGG_A
Cuts at: 2367 2367
Size: 5671
PmeI GTTT'AAAC
Cuts at: 2975 2975
Size: 5671
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3740 3772 5253 5285 3740
Size: 32 1481 32 4126
Fragments arranged by size:
4126 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2724 1613
Size: 1111 4560
PsiI TTA'TAA
Cuts at: 3241 3241
Size: 5671
PspOMI G'GGCC_C
Cuts at: 2441 2441
Size: 5671
PstI C_TGCA'G
Cuts at: 830 2046 2196 3023 830
Size: 1216 150 827 3478
Fragments arranged by size:
3478 1216 827 150
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5671
PvuII CAG'CTG
Cuts at: 2059 2588 2656 2059
Size: 529 68 5074
Fragments arranged by size:
5074 529 68
RsrII CG'GwC_CG
Cuts at: 2365 2365
Size: 5671
SacI G_AGCT'C
Cuts at: 2910 2910
Size: 5671
SalI G'TCGA_C
Cuts at: 1936 3013 1936
Size: 1077 4594
SapI GCTCTTCn'nnn_
Cuts at: 2400 2716 2751 2400
Size: 316 35 5320
Fragments arranged by size:
5320 316 35
SbfI CC_TGCA'GG
Cuts at: 3023 3023
Size: 5671
ScaI AGT'ACT
Cuts at: 3919 5631 3919
Size: 1712 3959
SfcI C'TryA_G
Cuts at: 826 1045 2042 2192 3019 4154 4835 5026 826
Size: 219 997 150 827 1135 681 191 1471
Fragments arranged by size:
1471 1135 997 827 681 219 191 150
SfiI GGCCn_nnn'nGGCC
Cuts at: 2117 2117
Size: 5671
SfoI GGC'GCC
Cuts at: 2146 2203 2146
Size: 57 5614
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5671
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5671
SmaI CCC'GGG
Cuts at: 1872 2330 2998 1872
Size: 458 668 4545
Fragments arranged by size:
4545 668 458
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2462 3775 4676 4938 820
Size: 197 926 519 1313 901 262 1553
Fragments arranged by size:
1553 1313 926 901 519 262 197
SnaBI TAC'GTA
Cuts at: 493 3462 493
Size: 2969 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5671
SphI G_CATG'C
Cuts at: 1132 3029 1132
Size: 1897 3774
SspI AAT'ATT
Cuts at: 5 52 3595 5
Size: 47 3543 2081
Fragments arranged by size:
3543 2081 47
StuI AGG'CCT
Cuts at: 1517 2111 1517
Size: 594 5077
StyI C'CwwG_G
Cuts at: 513 1065 2565 2677 3110 513
Size: 552 1500 112 433 3074
Fragments arranged by size:
3074 1500 552 433 112
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2795 3698 3715 3868 4053 4498 325
Size: 1255 1215 903 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1215 903 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3917 5629 96
Size: 274 80 33 51 1443 1940 1712 138
Fragments arranged by size:
1940 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5631 5663 5631
Size: 32 5639
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5671
XbaI T'CTAG_A
Cuts at: 3007 3007
Size: 5671
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2161 2161
Size: 5671
XhoI C'TCGA_G
Cuts at: 1943 2462 1943
Size: 519 5152
XmaI C'CCGG_G
Cuts at: 1870 2328 2996 1870
Size: 458 668 4545
Fragments arranged by size:
4545 668 458
XmnI GAAnn'nnTTC
Cuts at: 3800 3800
Size: 5671
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3479 276
Size: 53 83 186 936 1945 2468
Fragments arranged by size:
2468 1945 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I DraIII EcoNI EcoRV FseI
FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI
PspXI PsrI SacII SanDI SexAI SrfI SwaI