Restriction map for
pFN21AE0438
(Circular) (Six-base) MAPSORT of: pFN21AE0438.seq Check: 1773 from: 1 to: 5689
pFN21AE0438 5689 bp
With 182 enzymes: SgfI *
October 20, 2009 10:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2378 2671 2378
Size: 293 5396
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2748 3499 278
Size: 53 83 186 936 1212 751 2468
Fragments arranged by size:
2468 1212 936 751 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2606 3032 1937
Size: 669 426 4594
Fragments arranged by size:
4594 669 426
Acc65I G'GTAC_C
Cuts at: 3010 3010
Size: 5689
AclI AA'CG_TT
Cuts at: 1563 3816 4189 1563
Size: 2253 373 3063
Fragments arranged by size:
3063 2253 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2879 3752 5136 5404 1632
Size: 1247 873 1384 268 1917
Fragments arranged by size:
1917 1384 1247 873 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5492
AflIII A'CryG_T
Cuts at: 1203 4588 5565 1203
Size: 3385 977 1327
Fragments arranged by size:
3385 1327 977
AgeI A'CCGG_T
Cuts at: 2011 4520 2011
Size: 2509 3180
AhdI GACnn_n'nnGTC
Cuts at: 4418 4418
Size: 5689
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5689
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5689
AlwNI CAG_nnn'CTG
Cuts at: 2801 3432 5004 2801
Size: 631 1572 3486
Fragments arranged by size:
3486 1572 631
ApaI G_GGCC'C
Cuts at: 2161 2161
Size: 5689
ApaLI G'TGCA_C
Cuts at: 3746 4902 3746
Size: 1156 4533
ApoI r'AATT_y
Cuts at: 1493 2585 2998 3224 3376 5485 1493
Size: 1092 413 226 152 2109 1697
Fragments arranged by size:
2109 1697 1092 413 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4243 160
Size: 872 3211 1606
Fragments arranged by size:
3211 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
AvaI C'yCGr_G
Cuts at: 1870 1943 2076 3014 1870
Size: 73 133 938 4545
Fragments arranged by size:
4545 938 133 73
BamHI G'GATC_C
Cuts at: 2202 2295 3019 3386 2202
Size: 93 724 367 4505
Fragments arranged by size:
4505 724 367 93
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2556 3010 4465 618
Size: 325 217 627 769 454 1455 1842
Fragments arranged by size:
1842 1455 769 627 454 325 217
BanII G_rGCy'C
Cuts at: 1391 2121 2161 2822 3008 1391
Size: 730 40 661 186 4072
Fragments arranged by size:
4072 730 661 186 40
BbeI G_GCGC'C
Cuts at: 2560 2560
Size: 5689
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4718
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3880 3914 1903
Size: 34 1943 34 3678
Fragments arranged by size:
3678 1943 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2040 3581 4791 2040
Size: 1541 1210 2938
Fragments arranged by size:
2938 1541 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5689
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2081 2437 4300
Size: 107 122 71 631 605 409 356 1863
Cuts at: 4300 136
Size: 1525
Fragments arranged by size:
1863 1525 631 605 409 356 122 107
71
BglII A'GATC_T
Cuts at: 5684 5684
Size: 5689
BlpI GC'TnA_GC
Cuts at: 3106 3106
Size: 5689
Bme1580I G_kGCm'C
Cuts at: 1790 2161 3750 4906 1790
Size: 371 1589 1156 2573
Fragments arranged by size:
2573 1589 1156 371
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5689
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3420 4378 442
Size: 416 53 83 399 24 2003 958 1753
Fragments arranged by size:
2003 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5689
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4349 1239
Size: 3110 2579
Bpu10I CC'TnA_GC
Cuts at: 2613 2613
Size: 5689
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2966 3814 4679 4977 2966
Size: 848 865 298 3678
Fragments arranged by size:
3678 865 848 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2002 4352 882
Size: 617 503 2350 2219
Fragments arranged by size:
2350 2219 617 503
BsaAI yAC'GTr
Cuts at: 493 1204 2191 3480 493
Size: 711 987 1289 2702
Fragments arranged by size:
2702 1289 987 711
BsaBI GATnn'nnATC
Cuts at: 5683 5683
Size: 5689
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2515 2557 2745 3496
Size: 53 83 186 936 982 42 188 751
Cuts at: 3496 3878 4513 275
Size: 382 635 1451
Fragments arranged by size:
1451 982 936 751 635 382 188 186
83 53 42
BsaWI w'CCGG_w
Cuts at: 2011 4122 4520 4794 4941 5352 2011
Size: 2111 398 274 147 411 2348
Fragments arranged by size:
2348 2111 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5464
Fragments arranged by size:
5464 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2168 1186
Size: 982 4707
BseYI C'CCAG_C
Cuts at: 2485 4892 5388 2485
Size: 2407 496 2786
Fragments arranged by size:
2786 2407 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5116
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3139 3900 4928 1540
Size: 275 186 1138 761 1028 2301
Fragments arranged by size:
2301 1138 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2468 3750 3835 4906 2468
Size: 1282 85 1071 3251
Fragments arranged by size:
3251 1282 1071 85
BsmI GAATG_Cn'
Cuts at: 3200 3293 3200
Size: 93 5596
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2121 2161 2468 2822 3008 3750 3835
Size: 399 331 40 307 354 186 742 85
Cuts at: 3835 4906 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 354 331 307 186
85 40
BspEI T'CCGG_A
Cuts at: 5352 5352
Size: 5689
BspHI T'CATG_A
Cuts at: 2022 3578 5308 2022
Size: 1556 1730 2403
Fragments arranged by size:
2403 1730 1556
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2378 2671 3044 844
Size: 1534 293 373 3489
Fragments arranged by size:
3489 1534 373 293
BsrBI CCG'CTC
Cuts at: 1394 1823 3576 1394
Size: 429 1753 3507
Fragments arranged by size:
3507 1753 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2505 4184 4358 65
Size: 1183 1257 1679 174 1396
Fragments arranged by size:
1679 1396 1257 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2011 2376 4333 4520 1700
Size: 110 201 365 1957 187 2869
Fragments arranged by size:
2869 1957 365 201 187 110
BsrGI T'GTAC_A
Cuts at: 96 2547 96
Size: 2451 3238
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5689
BssSI C'ACGA_G
Cuts at: 2422 3749 4761 2422
Size: 1327 1012 3350
Fragments arranged by size:
3350 1327 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5689
BstBI TT'CG_AA
Cuts at: 4493 4493
Size: 5689
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5689
BstXI CCAn_nnnn'nTGG
Cuts at: 2251 2251
Size: 5689
BstYI r'GATC_y
Cuts at: 1972 2202 2295 3019 3386 3771 3788 5240 5326
Size: 230 93 724 367 385 17 1452 86
Cuts at: 5326 5338 5684 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 724 385 367 346 230 93
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2571 2571
Size: 5689
BtgI C'CryG_G
Cuts at: 513 1065 2161 513
Size: 552 1096 4041
Fragments arranged by size:
4041 1096 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4950
Fragments arranged by size:
4950 683 56
BtsI GCAGTG_nn'
Cuts at: 2108 2828 2896 3205 3999 4019 2108
Size: 720 68 309 794 20 3778
Fragments arranged by size:
3778 794 720 309 68 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5689
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2272 2307 529
Size: 35 1708 35 3911
Fragments arranged by size:
3911 1708 35 35
DraI TTT'AAA
Cuts at: 2993 2993
Size: 5689
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4696 809
Size: 507 3380 1802
Fragments arranged by size:
3380 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2778 3136 4025
Size: 54 1425 50 275 18 948 358 889
Cuts at: 4025 5654 8
Size: 1629 43
Fragments arranged by size:
1629 1425 948 889 358 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3136 1537
Size: 275 1324 4090
Fragments arranged by size:
4090 1324 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3619 1353
Size: 2266 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4276 4650 4796 387
Size: 1064 138 2687 374 146 1280
Fragments arranged by size:
2687 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2879 3752 4349 5136 5404 1239
Size: 393 1247 873 597 787 268 1524
Fragments arranged by size:
1524 1247 873 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2768 2768
Size: 5689
EcoO109I rG'GnC_Cy
Cuts at: 2157 2157
Size: 5689
EcoRI G'AATT_C
Cuts at: 2585 2998 3376 2585
Size: 413 378 4898
Fragments arranged by size:
4898 413 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2710 2710
Size: 5689
FspI TGC'GCA
Cuts at: 4195 4195
Size: 5689
HaeII r_GCGC'y
Cuts at: 1678 2560 4836 1678
Size: 882 2276 2531
Fragments arranged by size:
2531 2276 882
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2588 2620 4330 4362 4404
Size: 32 135 32 1016 32 1710 32 42
Cuts at: 4404 4436 1373
Size: 32 2626
Fragments arranged by size:
2626 1710 1016 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3033 3279 669
Size: 988 281 1095 246 3079
Fragments arranged by size:
3079 1095 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5689
HpaI GTT'AAC
Cuts at: 3279 3279
Size: 5689
KasI G'GCGC_C
Cuts at: 2556 2556
Size: 5689
KpnI G_GTAC'C
Cuts at: 3014 3014
Size: 5689
MfeI C'AATT_G
Cuts at: 3288 3288
Size: 5689
MluI A'CGCG_T
Cuts at: 5565 5565
Size: 5689
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2208 4803 4987 1305
Size: 903 2595 184 2007
Fragments arranged by size:
2595 2007 903 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2780 10
Size: 54 1425 343 948 2919
Fragments arranged by size:
2919 1425 948 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2027 2249 2642 3647 4006 4165 518
Size: 648 861 222 393 1005 359 159 2042
Fragments arranged by size:
2042 1005 861 648 393 359 222 159
MspA1I CmG'CkG
Cuts at: 1606 2582 2717 2798 3105 3782 4930 5175 1606
Size: 976 135 81 307 677 1148 245 2120
Fragments arranged by size:
2120 1148 976 677 307 245 135 81
NarI GG'CG_CC
Cuts at: 2557 2557
Size: 5689
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5137
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5689
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5689
NotI GC'GGCC_GC
Cuts at: 3136 3136
Size: 5689
NspI r_CATG'y
Cuts at: 1132 1229 3047 4592 1132
Size: 97 1818 1545 2229
Fragments arranged by size:
2229 1818 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5689
PciI A'CATG_T
Cuts at: 4588 4588
Size: 5689
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2776 1108
Size: 1668 4021
PfoI T'CCnGG_A
Cuts at: 2205 2205
Size: 5689
PmeI GTTT'AAAC
Cuts at: 2993 2993
Size: 5689
PmlI CAC'GTG
Cuts at: 2191 2191
Size: 5689
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3758 3790 5271 5303 3758
Size: 32 1481 32 4144
Fragments arranged by size:
4144 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5689
PsiI TTA'TAA
Cuts at: 3259 3259
Size: 5689
PspOMI G'GGCC_C
Cuts at: 2157 2157
Size: 5689
PstI C_TGCA'G
Cuts at: 830 2218 3041 830
Size: 1388 823 3478
Fragments arranged by size:
3478 1388 823
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5689
PvuII CAG'CTG
Cuts at: 2717 2798 2717
Size: 81 5608
SalI G'TCGA_C
Cuts at: 1936 3031 1936
Size: 1095 4594
SbfI CC_TGCA'GG
Cuts at: 3041 3041
Size: 5689
ScaI AGT'ACT
Cuts at: 3937 5649 3937
Size: 1712 3977
SfcI C'TryA_G
Cuts at: 826 1045 2214 3037 4172 4853 5044 826
Size: 219 1169 823 1135 681 191 1471
Fragments arranged by size:
1471 1169 1135 823 681 219 191
SfoI GGC'GCC
Cuts at: 2558 2558
Size: 5689
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5689
SmaI CCC'GGG
Cuts at: 1872 2078 3016 1872
Size: 206 938 4545
Fragments arranged by size:
4545 938 206
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2981 3793 4694 4956 820
Size: 197 926 1038 812 901 262 1553
Fragments arranged by size:
1553 1038 926 901 812 262 197
SnaBI TAC'GTA
Cuts at: 493 3480 493
Size: 2987 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5689
SphI G_CATG'C
Cuts at: 1132 3047 1132
Size: 1915 3774
SrfI GCCC'GGGC
Cuts at: 2078 2078
Size: 5689
SspI AAT'ATT
Cuts at: 5 52 3613 5
Size: 47 3561 2081
Fragments arranged by size:
3561 2081 47
StuI AGG'CCT
Cuts at: 1517 2569 2774 1517
Size: 1052 205 4432
Fragments arranged by size:
4432 1052 205
StyI C'CwwG_G
Cuts at: 513 1065 2130 2775 3128 513
Size: 552 1065 645 353 3074
Fragments arranged by size:
3074 1065 645 552 353
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2912 3716 3733 3886 4071 4516 325
Size: 1255 1332 804 17 153 185 445 1498
Fragments arranged by size:
1498 1332 1255 804 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2547 3935 5647
Size: 274 80 33 51 1443 570 1388 1712
Cuts at: 5647 96
Size: 138
Fragments arranged by size:
1712 1443 1388 570 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2796 2828 5649 5681 2796
Size: 32 2821 32 2804
Fragments arranged by size:
2821 2804 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5689
XbaI T'CTAG_A
Cuts at: 3025 3025
Size: 5689
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2923 2923
Size: 5689
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5689
XmaI C'CCGG_G
Cuts at: 1870 2076 3014 1870
Size: 206 938 4545
Fragments arranged by size:
4545 938 206
XmnI GAAnn'nnTTC
Cuts at: 2030 2525 3818 2030
Size: 495 1293 3901
Fragments arranged by size:
3901 1293 495
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2746 3497 276
Size: 53 83 186 936 1212 751 2468
Fragments arranged by size:
2468 1212 936 751 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI
NaeI NgoMIV NruI NsiI PacI PpuMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SwaI