Restriction map for
pFN21AE0436
(Circular) (Six-base) MAPSORT of: pFN21AE0436.seq Check: 4489 from: 1 to: 5797
pFN21AE0436 5797 bp
With 182 enzymes: SgfI *
October 20, 2009 10:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3607 278
Size: 53 83 186 936 2071 2468
Fragments arranged by size:
2468 2071 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2175 3140 1937
Size: 238 965 4594
Fragments arranged by size:
4594 965 238
Acc65I G'GTAC_C
Cuts at: 3118 3118
Size: 5797
AclI AA'CG_TT
Cuts at: 1563 2854 3924 4297 1563
Size: 1291 1070 373 3063
Fragments arranged by size:
3063 1291 1070 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3860 5244 5512 1632
Size: 2228 1384 268 1917
Fragments arranged by size:
2228 1917 1384 268
AfeI AGC'GCT
Cuts at: 2151 2151
Size: 5797
AflII C'TTAA_G
Cuts at: 820 1017 2986 820
Size: 197 1969 3631
Fragments arranged by size:
3631 1969 197
AflIII A'CryG_T
Cuts at: 1203 2028 2088 2344 2608 4696 5673 1203
Size: 825 60 256 264 2088 977 1327
Fragments arranged by size:
2088 1327 977 825 264 256 60
AgeI A'CCGG_T
Cuts at: 4628 4628
Size: 5797
AhdI GACnn_n'nnGTC
Cuts at: 4526 4526
Size: 5797
AleI CACnn'nnGTG
Cuts at: 1166 2087 1166
Size: 921 4876
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5797
AlwNI CAG_nnn'CTG
Cuts at: 3540 5112 3540
Size: 1572 4225
ApaLI G'TGCA_C
Cuts at: 3854 5010 3854
Size: 1156 4641
ApoI r'AATT_y
Cuts at: 1493 2441 2750 2775 2885 3106 3332 3484 5593
Size: 948 309 25 110 221 226 152 2109
Cuts at: 5593 1493
Size: 1697
Fragments arranged by size:
2109 1697 948 309 226 221 152 110
25
AseI AT'TA_AT
Cuts at: 160 1032 4351 160
Size: 872 3319 1606
Fragments arranged by size:
3319 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5797
AvaI C'yCGr_G
Cuts at: 1870 1943 3122 1870
Size: 73 1179 4545
Fragments arranged by size:
4545 1179 73
BamHI G'GATC_C
Cuts at: 3127 3494 3127
Size: 367 5430
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2077 3118 4573 618
Size: 325 217 627 290 1041 1455 1842
Fragments arranged by size:
1842 1455 1041 627 325 290 217
BanII G_rGCy'C
Cuts at: 1391 3116 1391
Size: 1725 4072
BbeI G_GCGC'C
Cuts at: 2081 2081
Size: 5797
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2969 928
Size: 971 1070 3756
Fragments arranged by size:
3756 1070 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3988 4022 1903
Size: 34 50 34 1967 34 3678
Fragments arranged by size:
3678 1967 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3689 4899 3689
Size: 1210 4587
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5797
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4408 136
Size: 107 122 71 631 605 2736 1525
Fragments arranged by size:
2736 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5792 5792
Size: 5797
BlpI GC'TnA_GC
Cuts at: 2406 3214 2406
Size: 808 4989
Bme1580I G_kGCm'C
Cuts at: 1790 2075 3858 5014 1790
Size: 285 1783 1156 2573
Fragments arranged by size:
2573 1783 1156 285
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5797
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3528 4486 442
Size: 416 53 83 399 24 2111 958 1753
Fragments arranged by size:
2111 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5797
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4457 1239
Size: 3218 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3922 4787 5085 3922
Size: 865 298 4634
Fragments arranged by size:
4634 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4460 882
Size: 617 2961 2219
Fragments arranged by size:
2961 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2031 2089 2356 2482 3588 493
Size: 711 827 58 267 126 1106 2702
Fragments arranged by size:
2702 1106 827 711 267 126 58
BsaBI GATnn'nnATC
Cuts at: 5791 5791
Size: 5797
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2078 3604 3986 4621
Size: 53 83 186 936 545 1526 382 635
Cuts at: 4621 275
Size: 1451
Fragments arranged by size:
1526 1451 936 635 545 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4230 4628 4902 5049 5460 4230
Size: 398 274 147 411 4567
Fragments arranged by size:
4567 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2018 2048 1178
Size: 30 165 30 615 30 4927
Fragments arranged by size:
4927 615 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5797
BseYI C'CCAG_C
Cuts at: 2054 5000 5496 2054
Size: 2946 496 2355
Fragments arranged by size:
2946 2355 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5224
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2161 3247 4008 5036 1540
Size: 275 186 160 1086 761 1028 2301
Fragments arranged by size:
2301 1086 1028 761 275 186 160
BsiHKAI G_wGCw'C
Cuts at: 2025 2067 2193 3858 3943 5014 2025
Size: 42 126 1665 85 1071 2808
Fragments arranged by size:
2808 1665 1071 126 85 42
BsmI GAATG_Cn'
Cuts at: 2338 3308 3401 2338
Size: 970 93 4734
Fragments arranged by size:
4734 970 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2025 2067 2075 2193 3116 3858 3943
Size: 399 235 42 8 118 923 742 85
Cuts at: 3943 5014 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 923 742 399 235 118 85
42 8
BspEI T'CCGG_A
Cuts at: 5460 5460
Size: 5797
BspHI T'CATG_A
Cuts at: 3686 5416 3686
Size: 1730 4067
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2055 3152 844
Size: 1211 1097 3489
Fragments arranged by size:
3489 1211 1097
BsrBI CCG'CTC
Cuts at: 1394 1823 3684 1394
Size: 429 1861 3507
Fragments arranged by size:
3507 1861 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4292 4466 65
Size: 1183 3044 174 1396
Fragments arranged by size:
3044 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2160 4441 4628 1700
Size: 110 350 2281 187 2869
Fragments arranged by size:
2869 2281 350 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5797
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5797
BssSI C'ACGA_G
Cuts at: 3857 4869 3857
Size: 1012 4785
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2579 794
Size: 1785 4012
BstBI TT'CG_AA
Cuts at: 2859 4601 2859
Size: 1742 4055
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5797
BstYI r'GATC_y
Cuts at: 1972 2902 3127 3494 3879 3896 5348 5434 5446
Size: 930 225 367 385 17 1452 86 12
Cuts at: 5446 5792 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 930 385 367 346 225 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5245
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5058
Fragments arranged by size:
5058 683 56
BtsI GCAGTG_nn'
Cuts at: 2440 3313 4107 4127 2440
Size: 873 794 20 4110
Fragments arranged by size:
4110 873 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5797
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5762
DraI TTT'AAA
Cuts at: 3101 3101
Size: 5797
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4804 809
Size: 507 3488 1802
Fragments arranged by size:
3488 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2319 3244 4133
Size: 54 1425 50 275 18 489 925 889
Cuts at: 4133 5762 8
Size: 1629 43
Fragments arranged by size:
1629 1425 925 889 489 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3244 1537
Size: 275 1432 4090
Fragments arranged by size:
4090 1432 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2198 2722 3727 1353
Size: 845 524 1005 3423
Fragments arranged by size:
3423 1005 845 524
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2110 4384 4758 4904 387
Size: 1064 138 521 2274 374 146 1280
Fragments arranged by size:
2274 1280 1064 521 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3860 4457 5244 5512 1239
Size: 393 2228 597 787 268 1524
Fragments arranged by size:
2228 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3106 3484 3106
Size: 378 5419
FspI TGC'GCA
Cuts at: 4303 4303
Size: 5797
HaeII r_GCGC'y
Cuts at: 1678 2081 2153 4944 1678
Size: 403 72 2791 2531
Fragments arranged by size:
2791 2531 403 72
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4438 4470 4512 4544 1373
Size: 32 135 32 2866 32 42 32 2626
Fragments arranged by size:
2866 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2296 3141 3387 669
Size: 988 281 358 845 246 3079
Fragments arranged by size:
3079 988 845 358 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5797
HpaI GTT'AAC
Cuts at: 3387 3387
Size: 5797
KasI G'GCGC_C
Cuts at: 2077 2077
Size: 5797
KpnI G_GTAC'C
Cuts at: 3122 3122
Size: 5797
MfeI C'AATT_G
Cuts at: 3396 3396
Size: 5797
MluI A'CGCG_T
Cuts at: 5673 5673
Size: 5797
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4911 5095 1305
Size: 3606 184 2007
Fragments arranged by size:
3606 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2321 10
Size: 54 1425 343 489 3486
Fragments arranged by size:
3486 1425 489 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2087 3011 3755 4114 4273 518
Size: 648 921 924 744 359 159 2042
Fragments arranged by size:
2042 924 921 744 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3213 3890 5038 5283 1606
Size: 1607 677 1148 245 2120
Fragments arranged by size:
2120 1607 1148 677 245
NarI GG'CG_CC
Cuts at: 2078 2078
Size: 5797
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5245
NdeI CA'TA_TG
Cuts at: 387 2832 387
Size: 2445 3352
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5797
NotI GC'GGCC_GC
Cuts at: 3244 3244
Size: 5797
NspI r_CATG'y
Cuts at: 1132 1229 2348 2612 3010 3155 4700 1132
Size: 97 1119 264 398 145 1545 2229
Fragments arranged by size:
2229 1545 1119 398 264 145 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5797
PciI A'CATG_T
Cuts at: 2344 2608 4696 2344
Size: 264 2088 3445
Fragments arranged by size:
3445 2088 264
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2152 2185 2390 1108
Size: 1044 33 205 4515
Fragments arranged by size:
4515 1044 205 33
PmeI GTTT'AAAC
Cuts at: 3101 3101
Size: 5797
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3866 3898 5379 5411 3866
Size: 32 1481 32 4252
Fragments arranged by size:
4252 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5797
PsiI TTA'TAA
Cuts at: 3367 3367
Size: 5797
PstI C_TGCA'G
Cuts at: 830 3004 3149 830
Size: 2174 145 3478
Fragments arranged by size:
3478 2174 145
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5797
SalI G'TCGA_C
Cuts at: 1936 3139 1936
Size: 1203 4594
SapI GCTCTTCn'nnn_
Cuts at: 2198 2198
Size: 5797
SbfI CC_TGCA'GG
Cuts at: 3149 3149
Size: 5797
ScaI AGT'ACT
Cuts at: 4045 5757 4045
Size: 1712 4085
SfcI C'TryA_G
Cuts at: 826 1045 3000 3145 4280 4961 5152 826
Size: 219 1955 145 1135 681 191 1471
Fragments arranged by size:
1955 1471 1135 681 219 191 145
SfoI GGC'GCC
Cuts at: 2079 2079
Size: 5797
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5797
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5797
SmaI CCC'GGG
Cuts at: 1872 3124 1872
Size: 1252 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2986 3901 4802 5064 820
Size: 197 926 1043 915 901 262 1553
Fragments arranged by size:
1553 1043 926 915 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3588 493
Size: 3095 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5797
SphI G_CATG'C
Cuts at: 1132 3010 3155 1132
Size: 1878 145 3774
Fragments arranged by size:
3774 1878 145
SspI AAT'ATT
Cuts at: 5 52 3721 5
Size: 47 3669 2081
Fragments arranged by size:
3669 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5797
StyI C'CwwG_G
Cuts at: 513 1065 3236 513
Size: 552 2171 3074
Fragments arranged by size:
3074 2171 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2382 3032 3824 3841 3994 4179 4624
Size: 1255 802 650 792 17 153 185 445
Cuts at: 4624 325
Size: 1498
Fragments arranged by size:
1498 1255 802 792 650 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4043 5755 96
Size: 274 80 33 51 1443 2066 1712 138
Fragments arranged by size:
2066 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2018 2050 5757 5789 2018
Size: 32 3707 32 2026
Fragments arranged by size:
3707 2026 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5797
XbaI T'CTAG_A
Cuts at: 3133 3133
Size: 5797
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2089 2089
Size: 5797
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5797
XmaI C'CCGG_G
Cuts at: 1870 3122 1870
Size: 1252 4545
XmnI GAAnn'nnTTC
Cuts at: 3926 3926
Size: 5797
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3605 276
Size: 53 83 186 936 2071 2468
Fragments arranged by size:
2468 2071 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI
SfiI SrfI SwaI