Restriction map for
pFN21AE0434
(Circular) (Six-base) MAPSORT of: pFN21AE0434.seq Check: 5560 from: 1 to: 5650
pFN21AE0434 5650 bp
With 182 enzymes: SgfI *
October 20, 2009 10:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3460 278
Size: 53 83 186 936 1924 2468
Fragments arranged by size:
2468 1924 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2993 1937
Size: 1056 4594
Acc65I G'GTAC_C
Cuts at: 2971 2971
Size: 5650
AclI AA'CG_TT
Cuts at: 1563 3777 4150 1563
Size: 2214 373 3063
Fragments arranged by size:
3063 2214 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2379 3713 5097 5365 1632
Size: 747 1334 1384 268 1917
Fragments arranged by size:
1917 1384 1334 747 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5453
AflIII A'CryG_T
Cuts at: 1203 4549 5526 1203
Size: 3346 977 1327
Fragments arranged by size:
3346 1327 977
AgeI A'CCGG_T
Cuts at: 4481 4481
Size: 5650
AhdI GACnn_n'nnGTC
Cuts at: 4379 4379
Size: 5650
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5650
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5650
AlwNI CAG_nnn'CTG
Cuts at: 2231 3393 4965 2231
Size: 1162 1572 2916
Fragments arranged by size:
2916 1572 1162
ApaLI G'TGCA_C
Cuts at: 3707 4863 3707
Size: 1156 4494
ApoI r'AATT_y
Cuts at: 1493 2201 2785 2959 3185 3337 5446 1493
Size: 708 584 174 226 152 2109 1697
Fragments arranged by size:
2109 1697 708 584 226 174 152
AseI AT'TA_AT
Cuts at: 160 1032 4204 160
Size: 872 3172 1606
Fragments arranged by size:
3172 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5650
AvaI C'yCGr_G
Cuts at: 1870 1943 2975 1870
Size: 73 1032 4545
Fragments arranged by size:
4545 1032 73
BamHI G'GATC_C
Cuts at: 2719 2980 3347 2719
Size: 261 367 5022
Fragments arranged by size:
5022 367 261
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2191 2971 4426 618
Size: 325 217 627 404 780 1455 1842
Fragments arranged by size:
1842 1455 780 627 404 325 217
BanII G_rGCy'C
Cuts at: 1391 2969 1391
Size: 1578 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2044 928
Size: 971 145 4534
Fragments arranged by size:
4534 971 145
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3841 3875 1903
Size: 34 1904 34 3678
Fragments arranged by size:
3678 1904 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3542 4752 3542
Size: 1210 4440
BclI T'GATC_A
Cuts at: 1548 2665 1548
Size: 1117 4533
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4261 136
Size: 107 122 71 631 605 2589 1525
Fragments arranged by size:
2589 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2095 2731 5645 2095
Size: 636 2914 2100
Fragments arranged by size:
2914 2100 636
BlpI GC'TnA_GC
Cuts at: 3067 3067
Size: 5650
Bme1580I G_kGCm'C
Cuts at: 1790 3711 4867 1790
Size: 1921 1156 2573
Fragments arranged by size:
2573 1921 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5650
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3381 4339 442
Size: 416 53 83 399 24 1964 958 1753
Fragments arranged by size:
1964 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5650
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2787 4310 1239
Size: 1548 1523 2579
Fragments arranged by size:
2579 1548 1523
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2370 3775 4640 4938 2370
Size: 1405 865 298 3082
Fragments arranged by size:
3082 1405 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2055 4313 882
Size: 617 556 2258 2219
Fragments arranged by size:
2258 2219 617 556
BsaAI yAC'GTr
Cuts at: 493 1204 3441 493
Size: 711 2237 2702
Fragments arranged by size:
2702 2237 711
BsaBI GATnn'nnATC
Cuts at: 2897 5644 2897
Size: 2747 2903
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3457 3839 4474 275
Size: 53 83 186 936 1924 382 635 1451
Fragments arranged by size:
1924 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4083 4481 4755 4902 5313 4083
Size: 398 274 147 411 4420
Fragments arranged by size:
4420 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2763 2793 2934 2964 1178
Size: 30 165 30 1360 30 141 30 3864
Fragments arranged by size:
3864 1360 165 141 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2035 2281 2450 2462 2784 2787 1186
Size: 849 246 169 12 322 3 4049
Fragments arranged by size:
4049 849 322 246 169 12 3
BseYI C'CCAG_C
Cuts at: 4853 5349 4853
Size: 496 5154
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5077
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3100 3861 4889 1540
Size: 275 186 1099 761 1028 2301
Fragments arranged by size:
2301 1099 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2949 3711 3796 4867 2949
Size: 762 85 1071 3732
Fragments arranged by size:
3732 1071 762 85
BsmI GAATG_Cn'
Cuts at: 2509 2885 3161 3254 2509
Size: 376 276 93 4905
Fragments arranged by size:
4905 376 276 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2949 2969 3711 3796 4867 1391
Size: 399 1159 20 742 85 1071 2174
Fragments arranged by size:
2174 1159 1071 742 399 85 20
BspEI T'CCGG_A
Cuts at: 5313 5313
Size: 5650
BspHI T'CATG_A
Cuts at: 2845 3539 5269 2845
Size: 694 1730 3226
Fragments arranged by size:
3226 1730 694
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3005 844
Size: 2161 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3537 1394
Size: 429 1714 3507
Fragments arranged by size:
3507 1714 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4145 4319 65
Size: 1183 2897 174 1396
Fragments arranged by size:
2897 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4294 4481 1700
Size: 110 2484 187 2869
Fragments arranged by size:
2869 2484 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5650
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5650
BssSI C'ACGA_G
Cuts at: 3710 4722 3710
Size: 1012 4638
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2222 794
Size: 1428 4222
BstBI TT'CG_AA
Cuts at: 4454 4454
Size: 5650
BstEII G'GTnAC_C
Cuts at: 1184 2319 1184
Size: 1135 4515
BstXI CCAn_nnnn'nTGG
Cuts at: 2594 2594
Size: 5650
BstYI r'GATC_y
Cuts at: 1972 2095 2605 2719 2731 2980 3347 3732 3749
Size: 123 510 114 12 249 367 385 17
Cuts at: 3749 5201 5287 5299 5645 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 510 385 367 346 249 123
114 86 17 12 12
Bsu36I CC'TnA_GG
Cuts at: 2325 2445 2325
Size: 120 5530
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4159
Fragments arranged by size:
4159 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4911
Fragments arranged by size:
4911 683 56
BtsI GCAGTG_nn'
Cuts at: 3166 3960 3980 3166
Size: 794 20 4836
Fragments arranged by size:
4836 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5650
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5615
DraI TTT'AAA
Cuts at: 2954 2954
Size: 5650
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2069 4657 809
Size: 507 753 2588 1802
Fragments arranged by size:
2588 1802 753 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2877 3097 3986
Size: 54 1425 50 275 18 1047 220 889
Cuts at: 3986 5615 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1047 889 275 220 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3097 1537
Size: 275 1285 4090
Fragments arranged by size:
4090 1285 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3580 1353
Size: 2227 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4237 4611 4757 387
Size: 1064 138 2648 374 146 1280
Fragments arranged by size:
2648 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2379 2787 3713 4310 5097 5365 1239
Size: 393 747 408 926 597 787 268 1524
Fragments arranged by size:
1524 926 787 747 597 408 393 268
EcoRI G'AATT_C
Cuts at: 2959 3337 2959
Size: 378 5272
FspI TGC'GCA
Cuts at: 4156 4156
Size: 5650
HaeII r_GCGC'y
Cuts at: 1678 4797 1678
Size: 3119 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2122 2154 2273 2305 2339
Size: 32 135 32 550 32 119 32 34
Cuts at: 2339 2371 2827 2859 4291 4323 4365 4397 1373
Size: 32 456 32 1432 32 42 32 2626
Fragments arranged by size:
2626 1432 550 456 135 119 42 34
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2994 3240 669
Size: 988 281 1056 246 3079
Fragments arranged by size:
3079 1056 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5650
HpaI GTT'AAC
Cuts at: 3240 3240
Size: 5650
KpnI G_GTAC'C
Cuts at: 2975 2975
Size: 5650
MfeI C'AATT_G
Cuts at: 3249 3249
Size: 5650
MluI A'CGCG_T
Cuts at: 5526 5526
Size: 5650
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2098 4764 4948 1305
Size: 793 2666 184 2007
Fragments arranged by size:
2666 2007 793 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2879 10
Size: 54 1425 343 1047 2781
Fragments arranged by size:
2781 1425 1047 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2673 3608 3967 4126 518
Size: 648 1507 935 359 159 2042
Fragments arranged by size:
2042 1507 935 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2040 2068 3066 3743 4891 5136 1606
Size: 434 28 998 677 1148 245 2120
Fragments arranged by size:
2120 1148 998 677 434 245 28
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4159
Fragments arranged by size:
4159 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5650
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5650
NotI GC'GGCC_GC
Cuts at: 3097 3097
Size: 5650
NspI r_CATG'y
Cuts at: 1132 1229 2867 3008 4553 1132
Size: 97 1638 141 1545 2229
Fragments arranged by size:
2229 1638 1545 141 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5650
PciI A'CATG_T
Cuts at: 4549 4549
Size: 5650
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2514 1108
Size: 1406 4244
PmeI GTTT'AAAC
Cuts at: 2954 2954
Size: 5650
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3719 3751 5232 5264 3719
Size: 32 1481 32 4105
Fragments arranged by size:
4105 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5650
PsiI TTA'TAA
Cuts at: 3220 3220
Size: 5650
PstI C_TGCA'G
Cuts at: 830 2218 3002 830
Size: 1388 784 3478
Fragments arranged by size:
3478 1388 784
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5650
PvuII CAG'CTG
Cuts at: 2040 2068 2040
Size: 28 5622
SalI G'TCGA_C
Cuts at: 1936 2992 1936
Size: 1056 4594
SbfI CC_TGCA'GG
Cuts at: 3002 3002
Size: 5650
ScaI AGT'ACT
Cuts at: 2650 3898 5610 2650
Size: 1248 1712 2690
Fragments arranged by size:
2690 1712 1248
SfcI C'TryA_G
Cuts at: 826 1045 2214 2472 2998 4133 4814 5005 826
Size: 219 1169 258 526 1135 681 191 1471
Fragments arranged by size:
1471 1169 1135 681 526 258 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5650
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5650
SmaI CCC'GGG
Cuts at: 1872 2977 1872
Size: 1105 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2385 3754 4655 4917 820
Size: 197 926 442 1369 901 262 1553
Fragments arranged by size:
1553 1369 926 901 442 262 197
SnaBI TAC'GTA
Cuts at: 493 3441 493
Size: 2948 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5650
SphI G_CATG'C
Cuts at: 1132 2867 3008 1132
Size: 1735 141 3774
Fragments arranged by size:
3774 1735 141
SspI AAT'ATT
Cuts at: 5 52 3574 5
Size: 47 3522 2081
Fragments arranged by size:
3522 2081 47
StuI AGG'CCT
Cuts at: 1517 2928 1517
Size: 1411 4239
StyI C'CwwG_G
Cuts at: 513 1065 2004 2083 2548 3089 513
Size: 552 939 79 465 541 3074
Fragments arranged by size:
3074 939 552 541 465 79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2631 3677 3694 3847 4032 4477 325
Size: 1255 1051 1046 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1051 1046 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2648 3896 5608
Size: 274 80 33 51 1443 671 1248 1712
Cuts at: 5608 96
Size: 138
Fragments arranged by size:
1712 1443 1248 671 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2763 2795 5610 5642 2763
Size: 32 2815 32 2771
Fragments arranged by size:
2815 2771 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5650
XbaI T'CTAG_A
Cuts at: 2986 2986
Size: 5650
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2081 2731 2081
Size: 650 5000
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5650
XmaI C'CCGG_G
Cuts at: 1870 2975 1870
Size: 1105 4545
XmnI GAAnn'nnTTC
Cuts at: 3779 3779
Size: 5650
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3458 276
Size: 53 83 186 936 1924 2468
Fragments arranged by size:
2468 1924 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI