Restriction map for
pFN21AE0432
(Circular) (Six-base) MAPSORT of: pFN21AE0432.seq Check: 9536 from: 1 to: 5923
pFN21AE0432 5923 bp
With 182 enzymes: SgfI *
October 20, 2009 10:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3733 278
Size: 53 83 186 936 2197 2468
Fragments arranged by size:
2468 2197 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3266 1937
Size: 1329 4594
Acc65I G'GTAC_C
Cuts at: 2851 3244 2851
Size: 393 5530
AclI AA'CG_TT
Cuts at: 1563 4050 4423 1563
Size: 2487 373 3063
Fragments arranged by size:
3063 2487 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2073 2547 3986 5370 5638 1632
Size: 441 474 1439 1384 268 1917
Fragments arranged by size:
1917 1439 1384 474 441 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5726
AflIII A'CryG_T
Cuts at: 1203 2038 2834 4822 5799 1203
Size: 835 796 1988 977 1327
Fragments arranged by size:
1988 1327 977 835 796
AgeI A'CCGG_T
Cuts at: 4754 4754
Size: 5923
AhdI GACnn_n'nnGTC
Cuts at: 4652 4652
Size: 5923
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5923
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5923
AlwNI CAG_nnn'CTG
Cuts at: 2654 2711 3666 5238 2654
Size: 57 955 1572 3339
Fragments arranged by size:
3339 1572 955 57
ApaLI G'TGCA_C
Cuts at: 3980 5136 3980
Size: 1156 4767
ApoI r'AATT_y
Cuts at: 1493 2071 2364 2681 2781 2919 3232 3458 3610
Size: 578 293 317 100 138 313 226 152
Cuts at: 3610 5719 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 578 317 313 293 226 152
138 100
AseI AT'TA_AT
Cuts at: 160 1032 4477 160
Size: 872 3445 1606
Fragments arranged by size:
3445 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5923
AvaI C'yCGr_G
Cuts at: 1870 1943 2730 3248 1870
Size: 73 787 518 4545
Fragments arranged by size:
4545 787 518 73
BamHI G'GATC_C
Cuts at: 3253 3620 3253
Size: 367 5556
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2851 3244 4699 618
Size: 325 217 627 1064 393 1455 1842
Fragments arranged by size:
1842 1455 1064 627 393 325 217
BanII G_rGCy'C
Cuts at: 1391 3242 1391
Size: 1851 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2364 928
Size: 971 465 4487
Fragments arranged by size:
4487 971 465
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4114 4148 1903
Size: 34 2177 34 3678
Fragments arranged by size:
3678 2177 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3815 5025 3815
Size: 1210 4713
BclI T'GATC_A
Cuts at: 1548 2494 1548
Size: 946 4977
BfrBI ATG'CAT
Cuts at: 2908 2908
Size: 5923
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2732 2741 4534
Size: 107 122 71 631 605 1060 9 1793
Cuts at: 4534 136
Size: 1525
Fragments arranged by size:
1793 1525 1060 631 605 122 107 71
9
BglII A'GATC_T
Cuts at: 5918 5918
Size: 5923
BlpI GC'TnA_GC
Cuts at: 3340 3340
Size: 5923
Bme1580I G_kGCm'C
Cuts at: 1790 2525 3074 3984 5140 1790
Size: 735 549 910 1156 2573
Fragments arranged by size:
2573 1156 910 735 549
BmgBI CAC'GTC
Cuts at: 1318 2039 1318
Size: 721 5202
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3654 4612 442
Size: 416 53 83 399 24 2237 958 1753
Fragments arranged by size:
2237 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5923
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3050 4583 1239
Size: 1811 1533 2579
Fragments arranged by size:
2579 1811 1533
Bpu10I CC'TnA_GC
Cuts at: 2359 2359
Size: 5923
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4048 4913 5211 4048
Size: 865 298 4760
Fragments arranged by size:
4760 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4586 882
Size: 617 3087 2219
Fragments arranged by size:
3087 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2280 3714 493
Size: 711 1076 1434 2702
Fragments arranged by size:
2702 1434 1076 711
BsaBI GATnn'nnATC
Cuts at: 5917 5917
Size: 5923
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3730 4112 4747 275
Size: 53 83 186 936 2197 382 635 1451
Fragments arranged by size:
2197 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4356 4754 5028 5175 5586 4356
Size: 398 274 147 411 4693
Fragments arranged by size:
4693 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2711 2741 1178
Size: 30 165 30 1308 30 4360
Fragments arranged by size:
4360 1308 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2122 3224 1186
Size: 936 1102 3885
Fragments arranged by size:
3885 1102 936
BseYI C'CCAG_C
Cuts at: 5126 5622 5126
Size: 496 5427
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2381 2697 1163
Size: 573 645 316 4389
Fragments arranged by size:
4389 645 573 316
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3373 4134 5162 1540
Size: 275 186 1372 761 1028 2301
Fragments arranged by size:
2301 1372 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3984 4069 5140 3984
Size: 85 1071 4767
Fragments arranged by size:
4767 1071 85
BsmI GAATG_Cn'
Cuts at: 2462 3434 3527 2462
Size: 972 93 4858
Fragments arranged by size:
4858 972 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2029 2029
Size: 5923
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2525 3074 3242 3984 4069 5140 1391
Size: 399 735 549 168 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 735 549 399 168 85
BspEI T'CCGG_A
Cuts at: 5586 5586
Size: 5923
BspHI T'CATG_A
Cuts at: 3812 5542 3812
Size: 1730 4193
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3278 844
Size: 2434 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2116 2155 3810 1394
Size: 429 293 39 1655 3507
Fragments arranged by size:
3507 1655 429 293 39
BsrDI GCAATG_nn'
Cuts at: 65 1248 2052 4418 4592 65
Size: 1183 804 2366 174 1396
Fragments arranged by size:
2366 1396 1183 804 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4567 4754 1700
Size: 110 2757 187 2869
Fragments arranged by size:
2869 2757 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5923
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5923
BssSI C'ACGA_G
Cuts at: 3983 4995 3983
Size: 1012 4911
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2711 794
Size: 1917 4006
BstBI TT'CG_AA
Cuts at: 4727 4727
Size: 5923
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5923
BstYI r'GATC_y
Cuts at: 1972 3253 3620 4005 4022 5474 5560 5572 5918
Size: 1281 367 385 17 1452 86 12 346
Cuts at: 5918 1972
Size: 1977
Fragments arranged by size:
1977 1452 1281 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3162 513
Size: 552 939 1158 3274
Fragments arranged by size:
3274 1158 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5184
Fragments arranged by size:
5184 683 56
BtsI GCAGTG_nn'
Cuts at: 3439 4233 4253 3439
Size: 794 20 5109
Fragments arranged by size:
5109 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5923
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5888
DraI TTT'AAA
Cuts at: 3227 3227
Size: 5923
DraIII CAC_nnn'GTG
Cuts at: 2953 2953
Size: 5923
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4930 809
Size: 507 3614 1802
Fragments arranged by size:
3614 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2628 3370 4259
Size: 54 1425 50 275 18 798 742 889
Cuts at: 4259 5888 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 798 742 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3370 1537
Size: 275 1558 4090
Fragments arranged by size:
4090 1558 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2092 2099 2511 2664 2682 3853 1353
Size: 739 7 412 153 18 1171 3423
Fragments arranged by size:
3423 1171 739 412 153 18 7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4510 4884 5030 387
Size: 1064 138 2921 374 146 1280
Fragments arranged by size:
2921 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2073 2547 3050 3986 4583 5370 5638
Size: 393 441 474 503 936 597 787 268
Cuts at: 5638 1239
Size: 1524
Fragments arranged by size:
1524 936 787 597 503 474 441 393
268
EcoRI G'AATT_C
Cuts at: 3232 3610 3232
Size: 378 5545
FspI TGC'GCA
Cuts at: 4429 4429
Size: 5923
HaeII r_GCGC'y
Cuts at: 1678 5070 1678
Size: 3392 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2139 2171 2491 2523 2711
Size: 32 135 32 567 32 320 32 188
Cuts at: 2711 2743 4564 4596 4638 4670 1373
Size: 32 1821 32 42 32 2626
Fragments arranged by size:
2626 1821 567 320 188 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3267 3513 669
Size: 988 281 1329 246 3079
Fragments arranged by size:
3079 1329 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3047 748
Size: 2299 3624
HpaI GTT'AAC
Cuts at: 3513 3513
Size: 5923
KpnI G_GTAC'C
Cuts at: 2855 3248 2855
Size: 393 5530
MfeI C'AATT_G
Cuts at: 3522 3522
Size: 5923
MluI A'CGCG_T
Cuts at: 5799 5799
Size: 5923
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2749 5037 5221 1305
Size: 1444 2288 184 2007
Fragments arranged by size:
2288 2007 1444 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2630 10
Size: 54 1425 343 798 3303
Fragments arranged by size:
3303 1425 798 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2064 2240 3881 4240 4399 518
Size: 648 898 176 1641 359 159 2042
Fragments arranged by size:
2042 1641 898 648 359 176 159
MspA1I CmG'CkG
Cuts at: 1606 3196 3339 4016 5164 5409 1606
Size: 1590 143 677 1148 245 2120
Fragments arranged by size:
2120 1590 1148 677 245 143
NcoI C'CATG_G
Cuts at: 513 1065 2004 3162 513
Size: 552 939 1158 3274
Fragments arranged by size:
3274 1158 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5923
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5923
NotI GC'GGCC_GC
Cuts at: 3370 3370
Size: 5923
NsiI A_TGCA'T
Cuts at: 2910 2910
Size: 5923
NspI r_CATG'y
Cuts at: 1132 1229 2584 2705 3281 4826 1132
Size: 97 1355 121 576 1545 2229
Fragments arranged by size:
2229 1545 1355 576 121 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5923
PciI A'CATG_T
Cuts at: 4822 4822
Size: 5923
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5923
PfoI T'CCnGG_A
Cuts at: 3098 3098
Size: 5923
PmeI GTTT'AAAC
Cuts at: 3227 3227
Size: 5923
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3992 4024 5505 5537 3992
Size: 32 1481 32 4378
Fragments arranged by size:
4378 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5923
PsiI TTA'TAA
Cuts at: 2930 3493 2930
Size: 563 5360
PstI C_TGCA'G
Cuts at: 830 3009 3275 830
Size: 2179 266 3478
Fragments arranged by size:
3478 2179 266
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5923
SalI G'TCGA_C
Cuts at: 1936 3265 1936
Size: 1329 4594
SbfI CC_TGCA'GG
Cuts at: 3275 3275
Size: 5923
ScaI AGT'ACT
Cuts at: 4171 5883 4171
Size: 1712 4211
SfcI C'TryA_G
Cuts at: 826 1045 3005 3271 4406 5087 5278 826
Size: 219 1960 266 1135 681 191 1471
Fragments arranged by size:
1960 1471 1135 681 266 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5923
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5923
SmaI CCC'GGG
Cuts at: 1872 2732 3250 1872
Size: 860 518 4545
Fragments arranged by size:
4545 860 518
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4027 4928 5190 820
Size: 197 926 2084 901 262 1553
Fragments arranged by size:
2084 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2280 3714 493
Size: 1787 1434 2702
Fragments arranged by size:
2702 1787 1434
SpeI A'CTAG_T
Cuts at: 152 2755 152
Size: 2603 3320
SphI G_CATG'C
Cuts at: 1132 3281 1132
Size: 2149 3774
SspI AAT'ATT
Cuts at: 5 52 2134 3847 5
Size: 47 2082 1713 2081
Fragments arranged by size:
2082 2081 1713 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5923
StyI C'CwwG_G
Cuts at: 513 1065 2004 3162 3362 513
Size: 552 939 1158 200 3074
Fragments arranged by size:
3074 1158 939 552 200
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3950 3967 4120 4305 4750 325
Size: 1255 2370 17 153 185 445 1498
Fragments arranged by size:
2370 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2758 4169 5881
Size: 274 80 33 51 1443 781 1411 1712
Cuts at: 5881 96
Size: 138
Fragments arranged by size:
1712 1443 1411 781 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5883 5915 5883
Size: 32 5891
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5923
XbaI T'CTAG_A
Cuts at: 3259 3259
Size: 5923
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5923
XmaI C'CCGG_G
Cuts at: 1870 2730 3248 1870
Size: 860 518 4545
Fragments arranged by size:
4545 860 518
XmnI GAAnn'nnTTC
Cuts at: 4052 4052
Size: 5923
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3731 276
Size: 53 83 186 936 2197 2468
Fragments arranged by size:
2468 2197 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI