Restriction map for
pFN21AE0428
(Circular) (Six-base) MAPSORT of: pFN21AE0428.seq Check: 7629 from: 1 to: 5602
pFN21AE0428 5602 bp
With 182 enzymes: SgfI *
October 20, 2009 10:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3412 278
Size: 53 83 186 936 1876 2468
Fragments arranged by size:
2468 1876 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2366 2945 1937
Size: 429 579 4594
Fragments arranged by size:
4594 579 429
Acc65I G'GTAC_C
Cuts at: 2923 2923
Size: 5602
AclI AA'CG_TT
Cuts at: 1563 3729 4102 1563
Size: 2166 373 3063
Fragments arranged by size:
3063 2166 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2615 2885 3665 5049 5317 1632
Size: 983 270 780 1384 268 1917
Fragments arranged by size:
1917 1384 983 780 270 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5405
AflIII A'CryG_T
Cuts at: 1203 2222 2514 4501 5478 1203
Size: 1019 292 1987 977 1327
Fragments arranged by size:
1987 1327 1019 977 292
AgeI A'CCGG_T
Cuts at: 4433 4433
Size: 5602
AhdI GACnn_n'nnGTC
Cuts at: 4331 4331
Size: 5602
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5602
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5602
AlwNI CAG_nnn'CTG
Cuts at: 2779 3345 4917 2779
Size: 566 1572 3464
Fragments arranged by size:
3464 1572 566
ApaLI G'TGCA_C
Cuts at: 3659 4815 3659
Size: 1156 4446
ApoI r'AATT_y
Cuts at: 1493 2399 2911 3137 3289 5398 1493
Size: 906 512 226 152 2109 1697
Fragments arranged by size:
2109 1697 906 512 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4156 160
Size: 872 3124 1606
Fragments arranged by size:
3124 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5602
AvaI C'yCGr_G
Cuts at: 1870 1943 2154 2927 1870
Size: 73 211 773 4545
Fragments arranged by size:
4545 773 211 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2069 2102 2069
Size: 33 5569
BamHI G'GATC_C
Cuts at: 2658 2932 3299 2658
Size: 274 367 4961
Fragments arranged by size:
4961 367 274
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2829 2923 4378 618
Size: 325 217 627 1042 94 1455 1842
Fragments arranged by size:
1842 1455 1042 627 325 217 94
BanII G_rGCy'C
Cuts at: 1391 2116 2921 1391
Size: 725 805 4072
Fragments arranged by size:
4072 805 725
BbeI G_GCGC'C
Cuts at: 2833 2833
Size: 5602
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2730 928
Size: 971 831 3800
Fragments arranged by size:
3800 971 831
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3793 3827 1903
Size: 34 1856 34 3678
Fragments arranged by size:
3678 1856 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3494 4704 3494
Size: 1210 4392
BclI T'GATC_A
Cuts at: 1548 2671 1548
Size: 1123 4479
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4213 136
Size: 107 122 71 631 605 2541 1525
Fragments arranged by size:
2541 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5597 5597
Size: 5602
BlpI GC'TnA_GC
Cuts at: 3019 3019
Size: 5602
Bme1580I G_kGCm'C
Cuts at: 1790 3663 4819 1790
Size: 1873 1156 2573
Fragments arranged by size:
2573 1873 1156
BmgBI CAC'GTC
Cuts at: 1318 2464 1318
Size: 1146 4456
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2125 3333 4291
Size: 416 53 83 399 24 708 1208 958
Cuts at: 4291 442
Size: 1753
Fragments arranged by size:
1753 1208 958 708 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5602
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2716 4262 1239
Size: 1477 1546 2579
Fragments arranged by size:
2579 1546 1477
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3727 4592 4890 3727
Size: 865 298 4439
Fragments arranged by size:
4439 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4265 882
Size: 617 2766 2219
Fragments arranged by size:
2766 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2225 3393 493
Size: 711 1021 1168 2702
Fragments arranged by size:
2702 1168 1021 711
BsaBI GATnn'nnATC
Cuts at: 5596 5596
Size: 5602
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2830 3409 3791 4426
Size: 53 83 186 936 1297 579 382 635
Cuts at: 4426 275
Size: 1451
Fragments arranged by size:
1451 1297 936 635 579 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2712 4035 4433 4707 4854 5265 2712
Size: 1323 398 274 147 411 3049
Fragments arranged by size:
3049 1323 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2768 2798 1178
Size: 30 165 30 1365 30 3982
Fragments arranged by size:
3982 1365 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2279 1186
Size: 1093 4509
BseYI C'CCAG_C
Cuts at: 4805 5301 4805
Size: 496 5106
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5029
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3052 3813 4841 1540
Size: 275 186 1051 761 1028 2301
Fragments arranged by size:
2301 1051 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3663 3748 4819 3663
Size: 85 1071 4446
Fragments arranged by size:
4446 1071 85
BsmI GAATG_Cn'
Cuts at: 3113 3206 3113
Size: 93 5509
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2116 2921 3663 3748 4819 1391
Size: 399 326 805 742 85 1071 2174
Fragments arranged by size:
2174 1071 805 742 399 326 85
BspEI T'CCGG_A
Cuts at: 5265 5265
Size: 5602
BspHI T'CATG_A
Cuts at: 2613 3491 5221 2613
Size: 878 1730 2994
Fragments arranged by size:
2994 1730 878
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2957 844
Size: 2113 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3489 1394
Size: 429 1666 3507
Fragments arranged by size:
3507 1666 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4097 4271 65
Size: 1183 2849 174 1396
Fragments arranged by size:
2849 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4246 4433 1700
Size: 110 2436 187 2869
Fragments arranged by size:
2869 2436 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5602
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5602
BssSI C'ACGA_G
Cuts at: 3662 4674 3662
Size: 1012 4590
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5602
BstBI TT'CG_AA
Cuts at: 4406 4406
Size: 5602
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5602
BstYI r'GATC_y
Cuts at: 1972 2658 2800 2932 3299 3684 3701 5153 5239
Size: 686 142 132 367 385 17 1452 86
Cuts at: 5239 5251 5597 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 686 385 367 346 142 132
86 17 12
BstZ17I GTA'TAC
Cuts at: 2367 2367
Size: 5602
Bsu36I CC'TnA_GG
Cuts at: 2027 2027
Size: 5602
BtgI C'CryG_G
Cuts at: 513 1065 2004 2301 513
Size: 552 939 297 3814
Fragments arranged by size:
3814 939 552 297
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4863
Fragments arranged by size:
4863 683 56
BtsI GCAGTG_nn'
Cuts at: 3118 3912 3932 3118
Size: 794 20 4788
Fragments arranged by size:
4788 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5602
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5567
DraI TTT'AAA
Cuts at: 2906 2906
Size: 5602
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4609 809
Size: 507 3293 1802
Fragments arranged by size:
3293 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2682 3049 3938
Size: 54 1425 50 275 18 852 367 889
Cuts at: 3938 5567 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 852 367 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3049 1537
Size: 275 1237 4090
Fragments arranged by size:
4090 1237 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3532 1353
Size: 2179 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4189 4563 4709 387
Size: 1064 138 2600 374 146 1280
Fragments arranged by size:
2600 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2615 2716 2885 3665 4262 5049 5317
Size: 393 983 101 169 780 597 787 268
Cuts at: 5317 1239
Size: 1524
Fragments arranged by size:
1524 983 787 780 597 393 268 169
101
EcoNI CCTnn'n_nnAGG
Cuts at: 2094 2094
Size: 5602
EcoO109I rG'GnC_Cy
Cuts at: 2315 2315
Size: 5602
EcoRI G'AATT_C
Cuts at: 2399 2911 3289 2399
Size: 512 378 4712
Fragments arranged by size:
4712 512 378
FspI TGC'GCA
Cuts at: 2360 4108 2360
Size: 1748 3854
HaeII r_GCGC'y
Cuts at: 1678 2833 4749 1678
Size: 1155 1916 2531
Fragments arranged by size:
2531 1916 1155
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4243 4275 4317 4349 1373
Size: 32 135 32 2671 32 42 32 2626
Fragments arranged by size:
2671 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2946 3192 669
Size: 988 281 1008 246 3079
Fragments arranged by size:
3079 1008 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2228 748
Size: 1480 4122
HpaI GTT'AAC
Cuts at: 3192 3192
Size: 5602
KasI G'GCGC_C
Cuts at: 2829 2829
Size: 5602
KpnI G_GTAC'C
Cuts at: 2927 2927
Size: 5602
MfeI C'AATT_G
Cuts at: 3201 3201
Size: 5602
MluI A'CGCG_T
Cuts at: 5478 5478
Size: 5602
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2903 4716 4900 1305
Size: 1598 1813 184 2007
Fragments arranged by size:
2007 1813 1598 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3780
Fragments arranged by size:
3780 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2628 3560 3919 4078 518
Size: 648 1462 932 359 159 2042
Fragments arranged by size:
2042 1462 932 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2200 2687 3018 3695 4843 5088 1606
Size: 594 487 331 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 594 487 331 245
NarI GG'CG_CC
Cuts at: 2830 2830
Size: 5602
NcoI C'CATG_G
Cuts at: 513 1065 2004 2301 513
Size: 552 939 297 3814
Fragments arranged by size:
3814 939 552 297
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5602
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5602
NotI GC'GGCC_GC
Cuts at: 3049 3049
Size: 5602
NspI r_CATG'y
Cuts at: 1132 1229 2163 2365 2518 2960 4505 1132
Size: 97 934 202 153 442 1545 2229
Fragments arranged by size:
2229 1545 934 442 202 153 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5602
PciI A'CATG_T
Cuts at: 2514 4501 2514
Size: 1987 3615
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5602
PfoI T'CCnGG_A
Cuts at: 2182 2182
Size: 5602
PmeI GTTT'AAAC
Cuts at: 2906 2906
Size: 5602
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3671 3703 5184 5216 3671
Size: 32 1481 32 4057
Fragments arranged by size:
4057 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2315 2315
Size: 5602
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5602
PsiI TTA'TAA
Cuts at: 2450 3172 2450
Size: 722 4880
PstI C_TGCA'G
Cuts at: 830 2954 830
Size: 2124 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5602
PvuII CAG'CTG
Cuts at: 2200 2200
Size: 5602
SalI G'TCGA_C
Cuts at: 1936 2944 1936
Size: 1008 4594
SbfI CC_TGCA'GG
Cuts at: 2954 2954
Size: 5602
ScaI AGT'ACT
Cuts at: 3850 5562 3850
Size: 1712 3890
SfcI C'TryA_G
Cuts at: 826 1045 2950 4085 4766 4957 826
Size: 219 1905 1135 681 191 1471
Fragments arranged by size:
1905 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2831 2831
Size: 5602
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5602
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5602
SmaI CCC'GGG
Cuts at: 1872 2929 1872
Size: 1057 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3706 4607 4869 820
Size: 197 926 1763 901 262 1553
Fragments arranged by size:
1763 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3393 493
Size: 2900 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5602
SphI G_CATG'C
Cuts at: 1132 2960 1132
Size: 1828 3774
SspI AAT'ATT
Cuts at: 5 52 3526 5
Size: 47 3474 2081
Fragments arranged by size:
3474 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5602
StyI C'CwwG_G
Cuts at: 513 1065 2004 2095 2301 3041 513
Size: 552 939 91 206 740 3074
Fragments arranged by size:
3074 939 740 552 206 91
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3629 3646 3799 3984 4429 325
Size: 1255 2049 17 153 185 445 1498
Fragments arranged by size:
2049 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2511 3848 5560
Size: 274 80 33 51 1443 534 1337 1712
Cuts at: 5560 96
Size: 138
Fragments arranged by size:
1712 1443 1337 534 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5562 5594 5562
Size: 32 5570
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5602
XbaI T'CTAG_A
Cuts at: 2938 2938
Size: 5602
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5602
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5602
XmaI C'CCGG_G
Cuts at: 1870 2927 1870
Size: 1057 4545
XmnI GAAnn'nnTTC
Cuts at: 2178 2403 3731 2178
Size: 225 1328 4049
Fragments arranged by size:
4049 1328 225
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3410 276
Size: 53 83 186 936 1876 2468
Fragments arranged by size:
2468 1876 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI DraIII EcoICRI EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SrfI SwaI