Restriction map for
pFN21AE0427
(Circular) (Six-base) MAPSORT of: pFN21AE0427.seq Check: 743 from: 1 to: 5239
pFN21AE0427 5239 bp
With 182 enzymes: SgfI *
October 20, 2009 10:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3049 278
Size: 53 83 186 936 1513 2468
Fragments arranged by size:
2468 1513 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2582 1937
Size: 645 4594
Acc65I G'GTAC_C
Cuts at: 2560 2560
Size: 5239
AclI AA'CG_TT
Cuts at: 1563 3366 3739 1563
Size: 1803 373 3063
Fragments arranged by size:
3063 1803 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2547 3302 4686 4954 1632
Size: 915 755 1384 268 1917
Fragments arranged by size:
1917 1384 915 755 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5042
AflIII A'CryG_T
Cuts at: 1203 2367 4138 5115 1203
Size: 1164 1771 977 1327
Fragments arranged by size:
1771 1327 1164 977
AgeI A'CCGG_T
Cuts at: 4070 4070
Size: 5239
AhdI GACnn_n'nnGTC
Cuts at: 3968 3968
Size: 5239
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5239
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5239
AlwNI CAG_nnn'CTG
Cuts at: 2982 4554 2982
Size: 1572 3667
ApaLI G'TGCA_C
Cuts at: 3296 4452 3296
Size: 1156 4083
ApoI r'AATT_y
Cuts at: 1493 2249 2548 2774 2926 5035 1493
Size: 756 299 226 152 2109 1697
Fragments arranged by size:
2109 1697 756 299 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2162 3793 160
Size: 872 1130 1631 1606
Fragments arranged by size:
1631 1606 1130 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5239
AvaI C'yCGr_G
Cuts at: 1870 1943 2564 1870
Size: 73 621 4545
Fragments arranged by size:
4545 621 73
BamHI G'GATC_C
Cuts at: 2065 2569 2936 2065
Size: 504 367 4368
Fragments arranged by size:
4368 504 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2146 2560 4015 618
Size: 325 217 627 359 414 1455 1842
Fragments arranged by size:
1842 1455 627 414 359 325 217
BanII G_rGCy'C
Cuts at: 1391 2558 1391
Size: 1167 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2535 928
Size: 971 636 3632
Fragments arranged by size:
3632 971 636
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3430 3464 1903
Size: 34 1493 34 3678
Fragments arranged by size:
3678 1493 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2473 3131 4341 2473
Size: 658 1210 3371
Fragments arranged by size:
3371 1210 658
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5239
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3850 136
Size: 107 122 71 631 605 2178 1525
Fragments arranged by size:
2178 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5234 5234
Size: 5239
BlpI GC'TnA_GC
Cuts at: 2656 2656
Size: 5239
Bme1580I G_kGCm'C
Cuts at: 1790 2223 3300 4456 1790
Size: 433 1077 1156 2573
Fragments arranged by size:
2573 1156 1077 433
BmgBI CAC'GTC
Cuts at: 1318 2224 1318
Size: 906 4333
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2970 3928 442
Size: 416 53 83 399 24 1553 958 1753
Fragments arranged by size:
1753 1553 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5239
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3899 1239
Size: 2660 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3364 4229 4527 3364
Size: 865 298 4076
Fragments arranged by size:
4076 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3902 882
Size: 617 2403 2219
Fragments arranged by size:
2403 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3030 493
Size: 711 1826 2702
Fragments arranged by size:
2702 1826 711
BsaBI GATnn'nnATC
Cuts at: 5233 5233
Size: 5239
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2257 3046 3428 4063
Size: 53 83 186 936 724 789 382 635
Cuts at: 4063 275
Size: 1451
Fragments arranged by size:
1451 936 789 724 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3672 4070 4344 4491 4902 3672
Size: 398 274 147 411 4009
Fragments arranged by size:
4009 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5014
Fragments arranged by size:
5014 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2309 1186
Size: 1123 4116
BseYI C'CCAG_C
Cuts at: 4442 4938 4442
Size: 496 4743
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4666
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2689 3450 4478 1540
Size: 275 186 688 761 1028 2301
Fragments arranged by size:
2301 1028 761 688 275 186
BsiHKAI G_wGCw'C
Cuts at: 3300 3385 4456 3300
Size: 85 1071 4083
Fragments arranged by size:
4083 1071 85
BsmI GAATG_Cn'
Cuts at: 2750 2843 2750
Size: 93 5146
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2223 2558 3300 3385 4456 1391
Size: 399 433 335 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 433 399 335 85
BspEI T'CCGG_A
Cuts at: 4902 4902
Size: 5239
BspHI T'CATG_A
Cuts at: 3128 4858 3128
Size: 1730 3509
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2036 2594 844
Size: 1192 558 3489
Fragments arranged by size:
3489 1192 558
BsrBI CCG'CTC
Cuts at: 1394 1823 3126 1394
Size: 429 1303 3507
Fragments arranged by size:
3507 1303 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3734 3908 65
Size: 1183 2486 174 1396
Fragments arranged by size:
2486 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3883 4070 1700
Size: 110 2073 187 2869
Fragments arranged by size:
2869 2073 187 110
BsrGI T'GTAC_A
Cuts at: 96 2370 96
Size: 2274 2965
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5239
BssSI C'ACGA_G
Cuts at: 3299 4311 3299
Size: 1012 4227
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5239
BstBI TT'CG_AA
Cuts at: 4043 4043
Size: 5239
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5239
BstYI r'GATC_y
Cuts at: 1972 2065 2329 2569 2936 3321 3338 4790 4876
Size: 93 264 240 367 385 17 1452 86
Cuts at: 4876 4888 5234 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 264 240 93
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2015 513
Size: 552 950 3737
Fragments arranged by size:
3737 950 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4500
Fragments arranged by size:
4500 683 56
BtsI GCAGTG_nn'
Cuts at: 2148 2755 3549 3569 2148
Size: 607 794 20 3818
Fragments arranged by size:
3818 794 607 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5239
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5204
DraI TTT'AAA
Cuts at: 2543 2543
Size: 5239
DraIII CAC_nnn'GTG
Cuts at: 2153 2153
Size: 5239
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4246 809
Size: 507 2930 1802
Fragments arranged by size:
2930 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2120 2421 2686
Size: 54 1425 50 275 18 290 301 265
Cuts at: 2686 3575 5204 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 301 290 275 265 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2686 1537
Size: 275 874 4090
Fragments arranged by size:
4090 874 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3169 1353
Size: 1816 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3826 4200 4346 387
Size: 1064 138 2237 374 146 1280
Fragments arranged by size:
2237 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2547 3302 3899 4686 4954 1239
Size: 393 915 755 597 787 268 1524
Fragments arranged by size:
1524 915 787 755 597 393 268
EcoRI G'AATT_C
Cuts at: 2548 2926 2548
Size: 378 4861
FspI TGC'GCA
Cuts at: 3745 3745
Size: 5239
HaeII r_GCGC'y
Cuts at: 1678 4386 1678
Size: 2708 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2477 2509 3880 3912 3954
Size: 32 135 32 905 32 1371 32 42
Cuts at: 3954 3986 1373
Size: 32 2626
Fragments arranged by size:
2626 1371 905 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2365 2583 2829 669
Size: 988 281 427 218 246 3079
Fragments arranged by size:
3079 988 427 281 246 218
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5239
HpaI GTT'AAC
Cuts at: 2365 2829 2365
Size: 464 4775
KpnI G_GTAC'C
Cuts at: 2564 2564
Size: 5239
MfeI C'AATT_G
Cuts at: 2838 2838
Size: 5239
MluI A'CGCG_T
Cuts at: 5115 5115
Size: 5239
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4353 4537 1305
Size: 3048 184 2007
Fragments arranged by size:
3048 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2122 10
Size: 54 1425 343 290 3127
Fragments arranged by size:
3127 1425 343 290 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3197 3556 3715 518
Size: 648 2031 359 159 2042
Fragments arranged by size:
2042 2031 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2655 3332 4480 4725 1606
Size: 1049 677 1148 245 2120
Fragments arranged by size:
2120 1148 1049 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4687
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5239
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5239
NotI GC'GGCC_GC
Cuts at: 2686 2686
Size: 5239
NspI r_CATG'y
Cuts at: 1132 1229 2371 2597 4142 1132
Size: 97 1142 226 1545 2229
Fragments arranged by size:
2229 1545 1142 226 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5239
PciI A'CATG_T
Cuts at: 2367 4138 2367
Size: 1771 3468
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5239
PmeI GTTT'AAAC
Cuts at: 2543 2543
Size: 5239
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3308 3340 4821 4853 3308
Size: 32 1481 32 3694
Fragments arranged by size:
3694 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5239
PsiI TTA'TAA
Cuts at: 2809 2809
Size: 5239
PstI C_TGCA'G
Cuts at: 830 2591 830
Size: 1761 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5239
SalI G'TCGA_C
Cuts at: 1936 2581 1936
Size: 645 4594
SbfI CC_TGCA'GG
Cuts at: 2591 2591
Size: 5239
ScaI AGT'ACT
Cuts at: 2414 3487 5199 2414
Size: 1073 1712 2454
Fragments arranged by size:
2454 1712 1073
SexAI A'CCwGG_T
Cuts at: 2149 2149
Size: 5239
SfcI C'TryA_G
Cuts at: 826 1045 2386 2587 3722 4403 4594 826
Size: 219 1341 201 1135 681 191 1471
Fragments arranged by size:
1471 1341 1135 681 219 201 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5239
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5239
SmaI CCC'GGG
Cuts at: 1872 2566 1872
Size: 694 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3343 4244 4506 820
Size: 197 926 1400 901 262 1553
Fragments arranged by size:
1553 1400 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3030 493
Size: 2537 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5239
SphI G_CATG'C
Cuts at: 1132 2597 1132
Size: 1465 3774
SspI AAT'ATT
Cuts at: 5 52 3163 5
Size: 47 3111 2081
Fragments arranged by size:
3111 2081 47
StuI AGG'CCT
Cuts at: 1517 2524 1517
Size: 1007 4232
StyI C'CwwG_G
Cuts at: 513 1065 2260 2678 513
Size: 552 1195 418 3074
Fragments arranged by size:
3074 1195 552 418
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3266 3283 3436 3621 4066 325
Size: 1255 1686 17 153 185 445 1498
Fragments arranged by size:
1686 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2370 2412 3485
Size: 274 80 33 51 1443 393 42 1073
Cuts at: 3485 5197 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1073 393 274 138 80 51
42 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5199 5231 5199
Size: 32 5207
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5239
XbaI T'CTAG_A
Cuts at: 2575 2575
Size: 5239
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5239
XmaI C'CCGG_G
Cuts at: 1870 2564 1870
Size: 694 4545
XmnI GAAnn'nnTTC
Cuts at: 3368 3368
Size: 5239
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3047 276
Size: 53 83 186 936 1513 2468
Fragments arranged by size:
2468 1513 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI