Restriction map for
pFN21AE0426
(Circular) (Six-base) MAPSORT of: pFN21AE0426.seq Check: 4737 from: 1 to: 5854
pFN21AE0426 5854 bp
With 182 enzymes: SgfI *
October 20, 2009 10:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2293 2293
Size: 5854
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2895 3664 278
Size: 53 83 186 936 1359 769 2468
Fragments arranged by size:
2468 1359 936 769 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2135 3197 1937
Size: 198 1062 4594
Fragments arranged by size:
4594 1062 198
Acc65I G'GTAC_C
Cuts at: 3175 3175
Size: 5854
AclI AA'CG_TT
Cuts at: 1563 3981 4354 1563
Size: 2418 373 3063
Fragments arranged by size:
3063 2418 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2349 2619 3917 5301 5569 1632
Size: 717 270 1298 1384 268 1917
Fragments arranged by size:
1917 1384 1298 717 270 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5657
AflIII A'CryG_T
Cuts at: 1203 4753 5730 1203
Size: 3550 977 1327
Fragments arranged by size:
3550 1327 977
AgeI A'CCGG_T
Cuts at: 4685 4685
Size: 5854
AhdI GACnn_n'nnGTC
Cuts at: 2359 4583 2359
Size: 2224 3630
AleI CACnn'nnGTG
Cuts at: 1166 2495 2687 1166
Size: 1329 192 4333
Fragments arranged by size:
4333 1329 192
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2705 2949 1184
Size: 1521 244 4089
Fragments arranged by size:
4089 1521 244
AlwNI CAG_nnn'CTG
Cuts at: 3597 5169 3597
Size: 1572 4282
ApaI G_GGCC'C
Cuts at: 2344 2344
Size: 5854
ApaLI G'TGCA_C
Cuts at: 3911 5067 3911
Size: 1156 4698
ApoI r'AATT_y
Cuts at: 1493 2657 2780 2832 3163 3389 3541 5650 1493
Size: 1164 123 52 331 226 152 2109 1697
Fragments arranged by size:
2109 1697 1164 331 226 152 123 52
AseI AT'TA_AT
Cuts at: 160 1032 4408 160
Size: 872 3376 1606
Fragments arranged by size:
3376 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5854
AvaI C'yCGr_G
Cuts at: 1870 1943 2645 3179 1870
Size: 73 702 534 4545
Fragments arranged by size:
4545 702 534 73
BamHI G'GATC_C
Cuts at: 2667 3184 3551 2667
Size: 517 367 4970
Fragments arranged by size:
4970 517 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2489 3030 3175 4630 618
Size: 325 217 627 702 541 145 1455 1842
Fragments arranged by size:
1842 1455 702 627 541 325 217 145
BanII G_rGCy'C
Cuts at: 1391 2025 2072 2344 2804 3173 1391
Size: 634 47 272 460 369 4072
Fragments arranged by size:
4072 634 460 369 272 47
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2355 928
Size: 971 456 4427
Fragments arranged by size:
4427 971 456
BbvCI CC'TCA_GC
Cuts at: 2321 2321
Size: 5854
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4045 4079 1903
Size: 34 2108 34 3678
Fragments arranged by size:
3678 2108 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3746 4956 3746
Size: 1210 4644
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5854
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4465 136
Size: 107 122 71 631 605 2793 1525
Fragments arranged by size:
2793 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5849 5849
Size: 5854
BlpI GC'TnA_GC
Cuts at: 2811 3271 2811
Size: 460 5394
Bme1580I G_kGCm'C
Cuts at: 1790 2083 2344 2694 3033 3915 5071 1790
Size: 293 261 350 339 882 1156 2573
Fragments arranged by size:
2573 1156 882 350 339 293 261
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5854
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2125 2200 3585
Size: 416 53 83 399 24 708 75 1385
Cuts at: 3585 4543 442
Size: 958 1753
Fragments arranged by size:
1753 1385 958 708 416 399 83 75
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5854
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4514 1239
Size: 3275 2579
Bpu10I CC'TnA_GC
Cuts at: 2321 2321
Size: 5854
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3979 4844 5142 3979
Size: 865 298 4691
Fragments arranged by size:
4691 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2897 4517 882
Size: 617 1398 1620 2219
Fragments arranged by size:
2219 1620 1398 617
BsaAI yAC'GTr
Cuts at: 493 1204 3645 493
Size: 711 2441 2702
Fragments arranged by size:
2702 2441 711
BsaBI GATnn'nnATC
Cuts at: 5848 5848
Size: 5854
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2892 3661 4043 4678
Size: 53 83 186 936 1359 769 382 635
Cuts at: 4678 275
Size: 1451
Fragments arranged by size:
1451 1359 936 769 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4287 4685 4959 5106 5517 4287
Size: 398 274 147 411 4624
Fragments arranged by size:
4624 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5629
Fragments arranged by size:
5629 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2015 3081 1186
Size: 829 1066 3959
Fragments arranged by size:
3959 1066 829
BseYI C'CCAG_C
Cuts at: 2937 5057 5553 2937
Size: 2120 496 3238
Fragments arranged by size:
3238 2120 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2467 1163
Size: 573 731 4550
Fragments arranged by size:
4550 731 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3304 4065 5093 1540
Size: 275 186 1303 761 1028 2301
Fragments arranged by size:
2301 1303 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3915 4000 5071 3915
Size: 85 1071 4698
Fragments arranged by size:
4698 1071 85
BsmI GAATG_Cn'
Cuts at: 3365 3458 3365
Size: 93 5761
BsmBI CGTCTCn'nnnn_
Cuts at: 2291 2291
Size: 5854
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2025 2072 2083 2344 2694 2804 3033
Size: 399 235 47 11 261 350 110 229
Cuts at: 3033 3173 3915 4000 5071 1391
Size: 140 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 350 261 235 229
140 110 85 47 11
BspEI T'CCGG_A
Cuts at: 5517 5517
Size: 5854
BspHI T'CATG_A
Cuts at: 3743 5473 3743
Size: 1730 4124
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2293 2400 3209 844
Size: 1449 107 809 3489
Fragments arranged by size:
3489 1449 809 107
BsrBI CCG'CTC
Cuts at: 1394 1823 3741 1394
Size: 429 1918 3507
Fragments arranged by size:
3507 1918 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2719 2933 4349 4523 65
Size: 1183 1471 214 1416 174 1396
Fragments arranged by size:
1471 1416 1396 1183 214 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2486 4498 4685 1700
Size: 110 676 2012 187 2869
Fragments arranged by size:
2869 2012 676 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5854
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5854
BssSI C'ACGA_G
Cuts at: 3914 4926 3914
Size: 1012 4842
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5854
BstBI TT'CG_AA
Cuts at: 4658 4658
Size: 5854
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5854
BstYI r'GATC_y
Cuts at: 1972 2667 3184 3551 3936 3953 5405 5491 5503
Size: 695 517 367 385 17 1452 86 12
Cuts at: 5503 5849 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 695 517 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2475 513
Size: 552 939 471 3892
Fragments arranged by size:
3892 939 552 471
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2946 487
Size: 683 56 1720 3395
Fragments arranged by size:
3395 1720 683 56
BtsI GCAGTG_nn'
Cuts at: 2225 2527 3370 4164 4184 2225
Size: 302 843 794 20 3895
Fragments arranged by size:
3895 843 794 302 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5854
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5819
DraI TTT'AAA
Cuts at: 2602 3158 2602
Size: 556 5298
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2208 4861 809
Size: 507 892 2653 1802
Fragments arranged by size:
2653 1802 892 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3301 4190 5819
Size: 54 1425 50 275 18 1471 889 1629
Cuts at: 5819 8
Size: 43
Fragments arranged by size:
1629 1471 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3301 1537
Size: 275 1489 4090
Fragments arranged by size:
4090 1489 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2033 2042 2235 2975 3784 1353
Size: 680 9 193 740 809 3423
Fragments arranged by size:
3423 809 740 680 193 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4441 4815 4961 387
Size: 1064 138 2852 374 146 1280
Fragments arranged by size:
2852 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2349 2619 3917 4514 5301 5569 1239
Size: 393 717 270 1298 597 787 268 1524
Fragments arranged by size:
1524 1298 787 717 597 393 270 268
EcoO109I rG'GnC_Cy
Cuts at: 2340 2340
Size: 5854
EcoRI G'AATT_C
Cuts at: 2832 3163 3541 2832
Size: 331 378 5145
Fragments arranged by size:
5145 378 331
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2176 2176
Size: 5854
FspI TGC'GCA
Cuts at: 4360 4360
Size: 5854
HaeII r_GCGC'y
Cuts at: 1678 5001 1678
Size: 3323 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2345 2377 4495 4527 4569
Size: 32 135 32 773 32 2118 32 42
Cuts at: 4569 4601 1373
Size: 32 2626
Fragments arranged by size:
2626 2118 773 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3198 3444 669
Size: 988 281 1260 246 3079
Fragments arranged by size:
3079 1260 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2570 748
Size: 1822 4032
HpaI GTT'AAC
Cuts at: 3444 3444
Size: 5854
KpnI G_GTAC'C
Cuts at: 3179 3179
Size: 5854
MfeI C'AATT_G
Cuts at: 3453 3453
Size: 5854
MluI A'CGCG_T
Cuts at: 5730 5730
Size: 5854
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2681 4968 5152 1305
Size: 1376 2287 184 2007
Fragments arranged by size:
2287 2007 1376 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4032
Fragments arranged by size:
4032 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2495 2687 2729 3812 4171 4330 518
Size: 648 1329 192 42 1083 359 159 2042
Fragments arranged by size:
2042 1329 1083 648 359 192 159 42
MspA1I CmG'CkG
Cuts at: 1606 2522 2818 2952 3270 3947 5095 5340 1606
Size: 916 296 134 318 677 1148 245 2120
Fragments arranged by size:
2120 1148 916 677 318 296 245 134
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4363
Fragments arranged by size:
4363 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5854
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5854
NotI GC'GGCC_GC
Cuts at: 3301 3301
Size: 5854
NspI r_CATG'y
Cuts at: 1132 1229 2513 3212 4757 1132
Size: 97 1284 699 1545 2229
Fragments arranged by size:
2229 1545 1284 699 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5854
PciI A'CATG_T
Cuts at: 4753 4753
Size: 5854
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2162 2700 3040 1108
Size: 1054 538 340 3922
Fragments arranged by size:
3922 1054 538 340
PfoI T'CCnGG_A
Cuts at: 2751 2751
Size: 5854
PmeI GTTT'AAAC
Cuts at: 3158 3158
Size: 5854
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3923 3955 5436 5468 3923
Size: 32 1481 32 4309
Fragments arranged by size:
4309 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5854
PsiI TTA'TAA
Cuts at: 3424 3424
Size: 5854
PspOMI G'GGCC_C
Cuts at: 2340 2340
Size: 5854
PstI C_TGCA'G
Cuts at: 830 3206 830
Size: 2376 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5854
PvuII CAG'CTG
Cuts at: 2522 2818 2522
Size: 296 5558
SalI G'TCGA_C
Cuts at: 1936 3196 1936
Size: 1260 4594
SbfI CC_TGCA'GG
Cuts at: 3206 3206
Size: 5854
ScaI AGT'ACT
Cuts at: 4102 5814 4102
Size: 1712 4142
SfcI C'TryA_G
Cuts at: 826 1045 3202 4337 5018 5209 826
Size: 219 2157 1135 681 191 1471
Fragments arranged by size:
2157 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5854
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5854
SmaI CCC'GGG
Cuts at: 1872 3181 1872
Size: 1309 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3958 4859 5121 820
Size: 197 926 2015 901 262 1553
Fragments arranged by size:
2015 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3645 493
Size: 3152 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5854
SphI G_CATG'C
Cuts at: 1132 3212 1132
Size: 2080 3774
SspI AAT'ATT
Cuts at: 5 52 3778 5
Size: 47 3726 2081
Fragments arranged by size:
3726 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5854
StyI C'CwwG_G
Cuts at: 513 1065 2004 2694 3293 513
Size: 552 939 690 599 3074
Fragments arranged by size:
3074 939 690 599 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2699 2874 3028 3881 3898 4051 4236
Size: 1255 1119 175 154 853 17 153 185
Cuts at: 4236 4681 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1119 853 445 185 175 154
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4100 5812 96
Size: 274 80 33 51 1443 2123 1712 138
Fragments arranged by size:
2123 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5814 5846 5814
Size: 32 5822
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5854
XbaI T'CTAG_A
Cuts at: 3190 3190
Size: 5854
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5854
XmaI C'CCGG_G
Cuts at: 1870 3179 1870
Size: 1309 4545
XmnI GAAnn'nnTTC
Cuts at: 2432 3983 2432
Size: 1551 4303
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2893 3662 276
Size: 53 83 186 936 1359 769 2468
Fragments arranged by size:
2468 1359 936 769 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI