Restriction map for
pFN21AE0425
(Circular) (Six-base) MAPSORT of: pFN21AE0425.seq Check: 8049 from: 1 to: 5824
pFN21AE0425 5824 bp
With 182 enzymes: SgfI *
October 20, 2009 10:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3117 3117
Size: 5824
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3634 278
Size: 53 83 186 936 2098 2468
Fragments arranged by size:
2468 2098 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3167 1937
Size: 1230 4594
Acc65I G'GTAC_C
Cuts at: 2638 3145 2638
Size: 507 5317
AclI AA'CG_TT
Cuts at: 1563 3951 4324 1563
Size: 2388 373 3063
Fragments arranged by size:
3063 2388 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2502 3887 5271 5539 1632
Size: 870 1385 1384 268 1917
Fragments arranged by size:
1917 1385 1384 870 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5627
AflIII A'CryG_T
Cuts at: 1203 4723 5700 1203
Size: 3520 977 1327
Fragments arranged by size:
3520 1327 977
AgeI A'CCGG_T
Cuts at: 4655 4655
Size: 5824
AhdI GACnn_n'nnGTC
Cuts at: 4553 4553
Size: 5824
AleI CACnn'nnGTG
Cuts at: 1166 2720 1166
Size: 1554 4270
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5824
AlwNI CAG_nnn'CTG
Cuts at: 2176 2275 3567 5139 2176
Size: 99 1292 1572 2861
Fragments arranged by size:
2861 1572 1292 99
ApaLI G'TGCA_C
Cuts at: 3881 5037 3881
Size: 1156 4668
ApoI r'AATT_y
Cuts at: 1493 2578 3133 3359 3511 5620 1493
Size: 1085 555 226 152 2109 1697
Fragments arranged by size:
2109 1697 1085 555 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4378 160
Size: 872 3346 1606
Fragments arranged by size:
3346 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5824
AvaI C'yCGr_G
Cuts at: 1870 1943 2769 3149 1870
Size: 73 826 380 4545
Fragments arranged by size:
4545 826 380 73
BamHI G'GATC_C
Cuts at: 3154 3521 3154
Size: 367 5457
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2375 2558 2638 3145 4600
Size: 325 217 627 588 183 80 507 1455
Cuts at: 4600 618
Size: 1842
Fragments arranged by size:
1842 1455 627 588 507 325 217 183
80
BanII G_rGCy'C
Cuts at: 1391 2141 2352 2820 3143 1391
Size: 750 211 468 323 4072
Fragments arranged by size:
4072 750 468 323 211
BbeI G_GCGC'C
Cuts at: 2562 2562
Size: 5824
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2490 928
Size: 971 591 4262
Fragments arranged by size:
4262 971 591
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2113 2147 3083 3117 4015 4049 1903
Size: 34 176 34 936 34 898 34 3678
Fragments arranged by size:
3678 936 898 176 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3716 4926 3716
Size: 1210 4614
BclI T'GATC_A
Cuts at: 1548 2943 1548
Size: 1395 4429
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2607 3038 4435
Size: 107 122 71 631 605 935 431 1397
Cuts at: 4435 136
Size: 1525
Fragments arranged by size:
1525 1397 935 631 605 431 122 107
71
BglII A'GATC_T
Cuts at: 2532 5819 2532
Size: 3287 2537
BlpI GC'TnA_GC
Cuts at: 3241 3241
Size: 5824
Bme1580I G_kGCm'C
Cuts at: 1790 3885 5041 1790
Size: 2095 1156 2573
Fragments arranged by size:
2573 2095 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5824
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2792 2905 3555
Size: 416 53 83 399 24 1375 113 650
Cuts at: 3555 4513 442
Size: 958 1753
Fragments arranged by size:
1753 1375 958 650 416 399 113 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5824
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2782 2782
Size: 5824
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4484 1239
Size: 3245 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3949 4814 5112 3949
Size: 865 298 4661
Fragments arranged by size:
4661 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2028 4487 882
Size: 617 529 2459 2219
Fragments arranged by size:
2459 2219 617 529
BsaAI yAC'GTr
Cuts at: 493 1204 3615 493
Size: 711 2411 2702
Fragments arranged by size:
2702 2411 711
BsaBI GATnn'nnATC
Cuts at: 2942 5818 2942
Size: 2876 2948
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2460 2559 3631 4013
Size: 53 83 186 936 927 99 1072 382
Cuts at: 4013 4648 275
Size: 635 1451
Fragments arranged by size:
1451 1072 936 927 635 382 186 99
83 53
BsaWI w'CCGG_w
Cuts at: 4257 4655 4929 5076 5487 4257
Size: 398 274 147 411 4594
Fragments arranged by size:
4594 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5599
Fragments arranged by size:
5599 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5824
BseYI C'CCAG_C
Cuts at: 2281 2408 5027 5523 2281
Size: 127 2619 496 2582
Fragments arranged by size:
2619 2582 496 127
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3113 1163
Size: 573 1377 3874
Fragments arranged by size:
3874 1377 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3274 4035 5063 1540
Size: 275 186 1273 761 1028 2301
Fragments arranged by size:
2301 1273 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2352 3885 3970 5041 2352
Size: 1533 85 1071 3135
Fragments arranged by size:
3135 1533 1071 85
BsmI GAATG_Cn'
Cuts at: 2086 3335 3428 2086
Size: 1249 93 4482
Fragments arranged by size:
4482 1249 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2141 2352 2820 3143 3885 3970 5041
Size: 399 351 211 468 323 742 85 1071
Cuts at: 5041 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 468 399 351 323 211
85
BspEI T'CCGG_A
Cuts at: 5487 5487
Size: 5824
BspHI T'CATG_A
Cuts at: 3713 5443 3713
Size: 1730 4094
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3117 3179 844
Size: 2273 62 3489
Fragments arranged by size:
3489 2273 62
BsrBI CCG'CTC
Cuts at: 1394 1823 3711 1394
Size: 429 1888 3507
Fragments arranged by size:
3507 1888 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4319 4493 65
Size: 1183 3071 174 1396
Fragments arranged by size:
3071 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4468 4655 1700
Size: 110 2658 187 2869
Fragments arranged by size:
2869 2658 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5824
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5824
BssSI C'ACGA_G
Cuts at: 3884 4896 3884
Size: 1012 4812
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5824
BstBI TT'CG_AA
Cuts at: 4628 4628
Size: 5824
BstEII G'GTnAC_C
Cuts at: 1184 2252 1184
Size: 1068 4756
BstYI r'GATC_y
Cuts at: 1972 2272 2532 3154 3521 3906 3923 5375 5461
Size: 300 260 622 367 385 17 1452 86
Cuts at: 5461 5473 5819 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 622 385 367 346 300 260
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2737 3042 3066 513
Size: 552 939 733 305 24 3271
Fragments arranged by size:
3271 939 733 552 305 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5085
Fragments arranged by size:
5085 683 56
BtsI GCAGTG_nn'
Cuts at: 2443 3340 4134 4154 2443
Size: 897 794 20 4113
Fragments arranged by size:
4113 897 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5824
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5789
DraI TTT'AAA
Cuts at: 3128 3128
Size: 5824
DraIII CAC_nnn'GTG
Cuts at: 2164 2786 2164
Size: 622 5202
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2839 4831 809
Size: 507 1523 1992 1802
Fragments arranged by size:
1992 1802 1523 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3271 4160 5789
Size: 54 1425 50 275 18 1441 889 1629
Cuts at: 5789 8
Size: 43
Fragments arranged by size:
1629 1441 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3271 1537
Size: 275 1459 4090
Fragments arranged by size:
4090 1459 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3754 1353
Size: 2401 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2588 4411 4785 4931 387
Size: 1064 138 999 1823 374 146 1280
Fragments arranged by size:
1823 1280 1064 999 374 146 138
EcoICRI GAG'CTC
Cuts at: 2350 2350
Size: 5824
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2502 3887 4484 5271 5539 1239
Size: 393 870 1385 597 787 268 1524
Fragments arranged by size:
1524 1385 870 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2651 2651
Size: 5824
EcoO109I rG'GnC_Cy
Cuts at: 2626 2872 2626
Size: 246 5578
EcoRI G'AATT_C
Cuts at: 3133 3511 3133
Size: 378 5446
EcoRV GAT'ATC
Cuts at: 2209 2467 2209
Size: 258 5566
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2368 2599 2368
Size: 231 5593
FspI TGC'GCA
Cuts at: 4330 4330
Size: 5824
HaeII r_GCGC'y
Cuts at: 1678 2562 4971 1678
Size: 884 2409 2531
Fragments arranged by size:
2531 2409 884
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2025 2057 2178 2210 4465
Size: 32 135 32 453 32 121 32 2255
Cuts at: 4465 4497 4539 4571 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2255 453 135 121 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2101 3168 3414 669
Size: 988 281 163 1067 246 3079
Fragments arranged by size:
3079 1067 988 281 246 163
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5824
HpaI GTT'AAC
Cuts at: 2101 3414 2101
Size: 1313 4511
KasI G'GCGC_C
Cuts at: 2558 2558
Size: 5824
KpnI G_GTAC'C
Cuts at: 2642 3149 2642
Size: 507 5317
MfeI C'AATT_G
Cuts at: 3423 3423
Size: 5824
MluI A'CGCG_T
Cuts at: 5700 5700
Size: 5824
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4938 5122 1305
Size: 3633 184 2007
Fragments arranged by size:
3633 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4002
Fragments arranged by size:
4002 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2720 2981 3782 4141 4300 518
Size: 648 1554 261 801 359 159 2042
Fragments arranged by size:
2042 1554 801 648 359 261 159
MspA1I CmG'CkG
Cuts at: 1606 2962 3021 3240 3917 5065 5310 1606
Size: 1356 59 219 677 1148 245 2120
Fragments arranged by size:
2120 1356 1148 677 245 219 59
NarI GG'CG_CC
Cuts at: 2559 2559
Size: 5824
NcoI C'CATG_G
Cuts at: 513 1065 2004 2737 3042 3066 513
Size: 552 939 733 305 24 3271
Fragments arranged by size:
3271 939 733 552 305 24
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5824
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5824
NotI GC'GGCC_GC
Cuts at: 3271 3271
Size: 5824
NspI r_CATG'y
Cuts at: 1132 1229 3182 4727 1132
Size: 97 1953 1545 2229
Fragments arranged by size:
2229 1953 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5824
PciI A'CATG_T
Cuts at: 4723 4723
Size: 5824
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5824
PmeI GTTT'AAAC
Cuts at: 3128 3128
Size: 5824
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3893 3925 5406 5438 3893
Size: 32 1481 32 4279
Fragments arranged by size:
4279 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2626 2626
Size: 5824
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5824
PsiI TTA'TAA
Cuts at: 3394 3394
Size: 5824
PstI C_TGCA'G
Cuts at: 830 2264 2967 3176 830
Size: 1434 703 209 3478
Fragments arranged by size:
3478 1434 703 209
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5824
PvuII CAG'CTG
Cuts at: 2962 3021 2962
Size: 59 5765
SacI G_AGCT'C
Cuts at: 2352 2352
Size: 5824
SalI G'TCGA_C
Cuts at: 1936 3166 1936
Size: 1230 4594
SanDI GG'GwC_CC
Cuts at: 2626 2626
Size: 5824
SbfI CC_TGCA'GG
Cuts at: 3176 3176
Size: 5824
ScaI AGT'ACT
Cuts at: 4072 5784 4072
Size: 1712 4112
SfcI C'TryA_G
Cuts at: 826 1045 2260 2963 3016 3172 4307 4988 5179
Size: 219 1215 703 53 156 1135 681 191
Cuts at: 5179 826
Size: 1471
Fragments arranged by size:
1471 1215 1135 703 681 219 191 156
53
SfoI GGC'GCC
Cuts at: 2560 2560
Size: 5824
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5824
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5824
SmaI CCC'GGG
Cuts at: 1872 3151 1872
Size: 1279 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3928 4829 5091 820
Size: 197 926 1985 901 262 1553
Fragments arranged by size:
1985 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3615 493
Size: 3122 2702
SpeI A'CTAG_T
Cuts at: 152 3058 152
Size: 2906 2918
SphI G_CATG'C
Cuts at: 1132 3182 1132
Size: 2050 3774
SspI AAT'ATT
Cuts at: 5 52 3748 5
Size: 47 3696 2081
Fragments arranged by size:
3696 2081 47
StuI AGG'CCT
Cuts at: 1517 2859 1517
Size: 1342 4482
StyI C'CwwG_G
Cuts at: 513 1065 2004 2621 2737 3042 3066 3263 513
Size: 552 939 617 116 305 24 197 3074
Fragments arranged by size:
3074 939 617 552 305 197 116 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2240 2774 2887 2995 3851 3868 4021
Size: 1255 660 534 113 108 856 17 153
Cuts at: 4021 4206 4651 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 856 660 534 445 185 153
113 108 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4070 5782 96
Size: 274 80 33 51 1443 2093 1712 138
Fragments arranged by size:
2093 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5784 5816 5784
Size: 32 5792
Tth111I GACn'n_nGTC
Cuts at: 1316 2039 1316
Size: 723 5101
XbaI T'CTAG_A
Cuts at: 3160 3160
Size: 5824
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3040 3040
Size: 5824
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5824
XmaI C'CCGG_G
Cuts at: 1870 3149 1870
Size: 1279 4545
XmnI GAAnn'nnTTC
Cuts at: 2487 2579 3953 2487
Size: 92 1374 4358
Fragments arranged by size:
4358 1374 92
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3632 276
Size: 53 83 186 936 2098 2468
Fragments arranged by size:
2468 2098 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I FseI FspAI
NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SacII SapI SexAI SfiI SrfI
SwaI