Restriction map for
pFN21AE0423
(Circular) (Six-base) MAPSORT of: pFN21AE0423.seq Check: 9548 from: 1 to: 5410
pFN21AE0423 5410 bp
With 182 enzymes: SgfI *
October 20, 2009 10:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3220 278
Size: 53 83 186 936 1684 2468
Fragments arranged by size:
2468 1684 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2753 1937
Size: 816 4594
Acc65I G'GTAC_C
Cuts at: 2731 2731
Size: 5410
AclI AA'CG_TT
Cuts at: 1563 3537 3910 1563
Size: 1974 373 3063
Fragments arranged by size:
3063 1974 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3473 4857 5125 1632
Size: 1841 1384 268 1917
Fragments arranged by size:
1917 1841 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5213
AflIII A'CryG_T
Cuts at: 1203 4309 5286 1203
Size: 3106 977 1327
Fragments arranged by size:
3106 1327 977
AgeI A'CCGG_T
Cuts at: 4241 4241
Size: 5410
AhdI GACnn_n'nnGTC
Cuts at: 4139 4139
Size: 5410
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5410
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5410
AlwNI CAG_nnn'CTG
Cuts at: 2272 3153 4725 2272
Size: 881 1572 2957
Fragments arranged by size:
2957 1572 881
ApaLI G'TGCA_C
Cuts at: 3467 4623 3467
Size: 1156 4254
ApoI r'AATT_y
Cuts at: 1493 2719 2945 3097 5206 1493
Size: 1226 226 152 2109 1697
Fragments arranged by size:
2109 1697 1226 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3964 160
Size: 872 2932 1606
Fragments arranged by size:
2932 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5410
AvaI C'yCGr_G
Cuts at: 1870 1943 2072 2289 2735 1870
Size: 73 129 217 446 4545
Fragments arranged by size:
4545 446 217 129 73
BamHI G'GATC_C
Cuts at: 2740 3107 2740
Size: 367 5043
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2250 2484 2525 2731 4186
Size: 325 217 627 463 234 41 206 1455
Cuts at: 4186 618
Size: 1842
Fragments arranged by size:
1842 1455 627 463 325 234 217 206
41
BanII G_rGCy'C
Cuts at: 1391 2409 2573 2729 1391
Size: 1018 164 156 4072
Fragments arranged by size:
4072 1018 164 156
BbeI G_GCGC'C
Cuts at: 2254 2254
Size: 5410
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4439
BbvCI CC'TCA_GC
Cuts at: 2139 2477 2639 2139
Size: 338 162 4910
Fragments arranged by size:
4910 338 162
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3601 3635 1903
Size: 34 1664 34 3678
Fragments arranged by size:
3678 1664 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3302 4512 3302
Size: 1210 4200
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5410
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4021 136
Size: 107 122 71 631 605 2349 1525
Fragments arranged by size:
2349 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5405 5405
Size: 5410
BlpI GC'TnA_GC
Cuts at: 2827 2827
Size: 5410
Bme1580I G_kGCm'C
Cuts at: 1790 2208 3471 4627 1790
Size: 418 1263 1156 2573
Fragments arranged by size:
2573 1263 1156 418
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5410
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3141 4099 442
Size: 416 53 83 399 24 1724 958 1753
Fragments arranged by size:
1753 1724 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5410
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2510 4070 1239
Size: 1271 1560 2579
Fragments arranged by size:
2579 1560 1271
Bpu10I CC'TnA_GC
Cuts at: 2139 2477 2572 2639 2139
Size: 338 95 67 4910
Fragments arranged by size:
4910 338 95 67
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3535 4400 4698 3535
Size: 865 298 4247
Fragments arranged by size:
4247 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2648 4073 882
Size: 617 1149 1425 2219
Fragments arranged by size:
2219 1425 1149 617
BsaAI yAC'GTr
Cuts at: 493 1204 2515 3201 493
Size: 711 1311 686 2702
Fragments arranged by size:
2702 1311 711 686
BsaBI GATnn'nnATC
Cuts at: 5404 5404
Size: 5410
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2251 2433 3217 3599
Size: 53 83 186 936 718 182 784 382
Cuts at: 3599 4234 275
Size: 635 1451
Fragments arranged by size:
1451 936 784 718 635 382 186 182
83 53
BsaWI w'CCGG_w
Cuts at: 3843 4241 4515 4662 5073 3843
Size: 398 274 147 411 4180
Fragments arranged by size:
4180 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2690 2720 1178
Size: 30 165 30 1287 30 3868
Fragments arranged by size:
3868 1287 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2051 2606 1186
Size: 865 555 3990
Fragments arranged by size:
3990 865 555
BseYI C'CCAG_C
Cuts at: 2121 2521 4613 5109 2121
Size: 400 2092 496 2422
Fragments arranged by size:
2422 2092 496 400
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2345 1163
Size: 573 609 4228
Fragments arranged by size:
4228 609 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2860 3621 4649 1540
Size: 275 186 859 761 1028 2301
Fragments arranged by size:
2301 1028 859 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2573 2669 3471 3556 4627 2573
Size: 96 802 85 1071 3356
Fragments arranged by size:
3356 1071 802 96 85
BsmI GAATG_Cn'
Cuts at: 2921 3014 2921
Size: 93 5317
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2208 2409 2573 2669 2729 3471 3556
Size: 399 418 201 164 96 60 742 85
Cuts at: 3556 4627 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 418 399 201 164 96
85 60
BspEI T'CCGG_A
Cuts at: 5073 5073
Size: 5410
BspHI T'CATG_A
Cuts at: 3299 5029 3299
Size: 1730 3680
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2765 844
Size: 1921 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3297 1394
Size: 429 1474 3507
Fragments arranged by size:
3507 1474 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2023 2133 3905 4079 65
Size: 1183 775 110 1772 174 1396
Fragments arranged by size:
1772 1396 1183 775 174 110
BsrFI r'CCGG_y
Cuts at: 1700 1810 2247 4054 4241 1700
Size: 110 437 1807 187 2869
Fragments arranged by size:
2869 1807 437 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5410
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5410
BssSI C'ACGA_G
Cuts at: 3470 4482 3470
Size: 1012 4398
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2518 794
Size: 1724 3686
BstBI TT'CG_AA
Cuts at: 4214 4214
Size: 5410
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5410
BstYI r'GATC_y
Cuts at: 1972 2740 3107 3492 3509 4961 5047 5059 5405
Size: 768 367 385 17 1452 86 12 346
Cuts at: 5405 1972
Size: 1977
Fragments arranged by size:
1977 1452 768 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2104 2209 513
Size: 552 1039 105 3714
Fragments arranged by size:
3714 1039 552 105
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4671
Fragments arranged by size:
4671 683 56
BtsI GCAGTG_nn'
Cuts at: 2050 2926 3720 3740 2050
Size: 876 794 20 3720
Fragments arranged by size:
3720 876 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5410
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5375
DraI TTT'AAA
Cuts at: 2714 2714
Size: 5410
DraIII CAC_nnn'GTG
Cuts at: 2518 2518
Size: 5410
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4417 809
Size: 507 3101 1802
Fragments arranged by size:
3101 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2175 2466 2857
Size: 54 1425 50 275 18 345 291 391
Cuts at: 2857 3746 5375 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 391 345 291 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2857 1537
Size: 275 1045 4090
Fragments arranged by size:
4090 1045 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2181 2336 3340 1353
Size: 828 155 1004 3423
Fragments arranged by size:
3423 1004 828 155
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3997 4371 4517 387
Size: 1064 138 2408 374 146 1280
Fragments arranged by size:
2408 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2571 2571
Size: 5410
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2510 3473 4070 4857 5125 1239
Size: 393 878 963 597 787 268 1524
Fragments arranged by size:
1524 963 878 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2642 2642
Size: 5410
EcoO109I rG'GnC_Cy
Cuts at: 2032 2191 2452 2599 2635 2032
Size: 159 261 147 36 4807
Fragments arranged by size:
4807 261 159 147 36
EcoRI G'AATT_C
Cuts at: 2719 3097 2719
Size: 378 5032
FspI TGC'GCA
Cuts at: 3916 3916
Size: 5410
HaeII r_GCGC'y
Cuts at: 1678 2254 4557 1678
Size: 576 2303 2531
Fragments arranged by size:
2531 2303 576
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2270 2302 2690 2722 4051
Size: 32 135 32 698 32 388 32 1329
Cuts at: 4051 4083 4125 4157 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1329 698 388 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2754 3000 669
Size: 988 281 816 246 3079
Fragments arranged by size:
3079 988 816 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5410
HpaI GTT'AAC
Cuts at: 3000 3000
Size: 5410
KasI G'GCGC_C
Cuts at: 2250 2250
Size: 5410
KpnI G_GTAC'C
Cuts at: 2735 2735
Size: 5410
MfeI C'AATT_G
Cuts at: 3009 3009
Size: 5410
MluI A'CGCG_T
Cuts at: 5286 5286
Size: 5410
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2397 2721 4524 4708 1305
Size: 1092 324 1803 184 2007
Fragments arranged by size:
2007 1803 1092 324 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2177 10
Size: 54 1425 343 345 3243
Fragments arranged by size:
3243 1425 345 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2462 3368 3727 3886 518
Size: 648 1296 906 359 159 2042
Fragments arranged by size:
2042 1296 906 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2826 3503 4651 4896 1606
Size: 1220 677 1148 245 2120
Fragments arranged by size:
2120 1220 1148 677 245
NarI GG'CG_CC
Cuts at: 2251 2251
Size: 5410
NcoI C'CATG_G
Cuts at: 513 1065 2104 2209 513
Size: 552 1039 105 3714
Fragments arranged by size:
3714 1039 552 105
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5410
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5410
NotI GC'GGCC_GC
Cuts at: 2857 2857
Size: 5410
NruI TCG'CGA
Cuts at: 2589 2589
Size: 5410
NspI r_CATG'y
Cuts at: 1132 1229 2047 2336 2768 4313 1132
Size: 97 818 289 432 1545 2229
Fragments arranged by size:
2229 1545 818 432 289 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5410
PciI A'CATG_T
Cuts at: 4309 4309
Size: 5410
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5410
PmeI GTTT'AAAC
Cuts at: 2714 2714
Size: 5410
PmlI CAC'GTG
Cuts at: 2515 2515
Size: 5410
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3479 3511 4992 5024 3479
Size: 32 1481 32 3865
Fragments arranged by size:
3865 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2032 2191 2452 2635 2032
Size: 159 261 183 4807
Fragments arranged by size:
4807 261 183 159
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5410
PsiI TTA'TAA
Cuts at: 2980 2980
Size: 5410
PstI C_TGCA'G
Cuts at: 830 2762 830
Size: 1932 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5410
SacI G_AGCT'C
Cuts at: 2573 2573
Size: 5410
SalI G'TCGA_C
Cuts at: 1936 2752 1936
Size: 816 4594
SbfI CC_TGCA'GG
Cuts at: 2762 2762
Size: 5410
ScaI AGT'ACT
Cuts at: 3658 5370 3658
Size: 1712 3698
SfcI C'TryA_G
Cuts at: 826 1045 2758 3893 4574 4765 826
Size: 219 1713 1135 681 191 1471
Fragments arranged by size:
1713 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2252 2252
Size: 5410
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5410
SgrAI Cr'CCGG_yG
Cuts at: 1700 2247 1700
Size: 547 4863
SmaI CCC'GGG
Cuts at: 1872 2737 1872
Size: 865 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2289 3514 4415 4677 820
Size: 197 926 346 1225 901 262 1553
Fragments arranged by size:
1553 1225 926 901 346 262 197
SnaBI TAC'GTA
Cuts at: 493 3201 493
Size: 2708 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5410
SphI G_CATG'C
Cuts at: 1132 2768 1132
Size: 1636 3774
SspI AAT'ATT
Cuts at: 5 52 3334 5
Size: 47 3282 2081
Fragments arranged by size:
3282 2081 47
StuI AGG'CCT
Cuts at: 1517 2285 2582 2693 1517
Size: 768 297 111 4234
Fragments arranged by size:
4234 768 297 111
StyI C'CwwG_G
Cuts at: 513 1065 2104 2209 2849 513
Size: 552 1039 105 640 3074
Fragments arranged by size:
3074 1039 640 552 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2246 2513 3437 3454 3607 3792 4237
Size: 1255 666 267 924 17 153 185 445
Cuts at: 4237 325
Size: 1498
Fragments arranged by size:
1498 1255 924 666 445 267 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3656 5368 96
Size: 274 80 33 51 1443 1679 1712 138
Fragments arranged by size:
1712 1679 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2354 2386 5370 5402 2354
Size: 32 2984 32 2362
Fragments arranged by size:
2984 2362 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5410
XbaI T'CTAG_A
Cuts at: 2746 2746
Size: 5410
XhoI C'TCGA_G
Cuts at: 1943 2289 1943
Size: 346 5064
XmaI C'CCGG_G
Cuts at: 1870 2735 1870
Size: 865 4545
XmnI GAAnn'nnTTC
Cuts at: 3539 3539
Size: 5410
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3218 276
Size: 53 83 186 936 1684 2468
Fragments arranged by size:
2468 1684 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NruI NspI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PstI PvuI SacI
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FalI
FseI FspAI NaeI NgoMIV NsiI PacI PfoI PspOMI
PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI
SfiI SrfI SwaI XcmI