Restriction map for
pFN21AE0422
(Circular) (Six-base) MAPSORT of: pFN21AE0422.seq Check: 6574 from: 1 to: 5884
pFN21AE0422 5884 bp
With 182 enzymes: SgfI *
October 20, 2009 10:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3141 3694 278
Size: 53 83 186 936 1605 553 2468
Fragments arranged by size:
2468 1605 936 553 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3227 1937
Size: 1290 4594
Acc65I G'GTAC_C
Cuts at: 3205 3205
Size: 5884
AclI AA'CG_TT
Cuts at: 1563 4011 4384 1563
Size: 2448 373 3063
Fragments arranged by size:
3063 2448 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2033 2218 2366 2591 3144 3947 5331 5599
Size: 401 185 148 225 553 803 1384 268
Cuts at: 5599 1632
Size: 1917
Fragments arranged by size:
1917 1384 803 553 401 268 225 185
148
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5687
AflIII A'CryG_T
Cuts at: 1203 4783 5760 1203
Size: 3580 977 1327
Fragments arranged by size:
3580 1327 977
AgeI A'CCGG_T
Cuts at: 4715 4715
Size: 5884
AhdI GACnn_n'nnGTC
Cuts at: 4613 4613
Size: 5884
AleI CACnn'nnGTG
Cuts at: 1166 2244 1166
Size: 1078 4806
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3051 1184
Size: 1867 4017
AlwNI CAG_nnn'CTG
Cuts at: 3627 5199 3627
Size: 1572 4312
ApaI G_GGCC'C
Cuts at: 2876 2876
Size: 5884
ApaLI G'TGCA_C
Cuts at: 2270 2660 3941 5097 2270
Size: 390 1281 1156 3057
Fragments arranged by size:
3057 1281 1156 390
ApoI r'AATT_y
Cuts at: 1493 3193 3419 3571 5680 1493
Size: 1700 226 152 2109 1697
Fragments arranged by size:
2109 1700 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4438 160
Size: 872 3406 1606
Fragments arranged by size:
3406 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5884
AvaI C'yCGr_G
Cuts at: 1870 1943 2054 2110 3209 1870
Size: 73 111 56 1099 4545
Fragments arranged by size:
4545 1099 111 73 56
BamHI G'GATC_C
Cuts at: 3214 3581 3214
Size: 367 5517
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2093 3205 4660 618
Size: 325 217 627 306 1112 1455 1842
Fragments arranged by size:
1842 1455 1112 627 325 306 217
BanII G_rGCy'C
Cuts at: 1391 2211 2876 3203 1391
Size: 820 665 327 4072
Fragments arranged by size:
4072 820 665 327
BbeI G_GCGC'C
Cuts at: 2097 2097
Size: 5884
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2224 928
Size: 971 325 4588
Fragments arranged by size:
4588 971 325
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2032 2066 2375 2409 2935 2969 4075
Size: 34 95 34 309 34 526 34 1106
Cuts at: 4075 4109 1903
Size: 34 3678
Fragments arranged by size:
3678 1106 526 309 95 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3776 4986 3776
Size: 1210 4674
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5884
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2045 2318 2460
Size: 107 122 71 631 605 373 273 142
Cuts at: 2460 3179 4495 136
Size: 719 1316 1525
Fragments arranged by size:
1525 1316 719 631 605 373 273 142
122 107 71
BglII A'GATC_T
Cuts at: 5879 5879
Size: 5884
BlpI GC'TnA_GC
Cuts at: 3301 3301
Size: 5884
Bme1580I G_kGCm'C
Cuts at: 1790 2274 2664 2876 3116 3945 5101 1790
Size: 484 390 212 240 829 1156 2573
Fragments arranged by size:
2573 1156 829 484 390 240 212
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5884
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2693 3615 4573
Size: 416 53 83 399 24 1276 922 958
Cuts at: 4573 442
Size: 1753
Fragments arranged by size:
1753 1276 958 922 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5884
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2125 2125
Size: 5884
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2555 2687 4544 1239
Size: 1316 132 1857 2579
Fragments arranged by size:
2579 1857 1316 132
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3005 4009 4874 5172 3005
Size: 1004 865 298 3717
Fragments arranged by size:
3717 1004 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4547 882
Size: 617 3048 2219
Fragments arranged by size:
3048 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2275 3675 493
Size: 711 1071 1400 2702
Fragments arranged by size:
2702 1400 1071 711
BsaBI GATnn'nnATC
Cuts at: 5878 5878
Size: 5884
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2094 3138 3691 4073
Size: 53 83 186 936 561 1044 553 382
Cuts at: 4073 4708 275
Size: 635 1451
Fragments arranged by size:
1451 1044 936 635 561 553 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3094 4317 4715 4989 5136 5547 3094
Size: 1223 398 274 147 411 3431
Fragments arranged by size:
3431 1223 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2125 2155 1178
Size: 30 165 30 722 30 4907
Fragments arranged by size:
4907 722 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5884
BseYI C'CCAG_C
Cuts at: 2796 2888 5087 5583 2796
Size: 92 2199 496 3097
Fragments arranged by size:
3097 2199 496 92
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3011 1163
Size: 573 1275 4036
Fragments arranged by size:
4036 1275 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2025 2658 3169 3334 4095 5123
Size: 275 186 24 633 511 165 761 1028
Cuts at: 5123 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 633 511 275 186 165
24
BsiHKAI G_wGCw'C
Cuts at: 2055 2211 2274 2664 3945 4030 5101 2055
Size: 156 63 390 1281 85 1071 2838
Fragments arranged by size:
2838 1281 1071 390 156 85 63
BsmI GAATG_Cn'
Cuts at: 3395 3488 3395
Size: 93 5791
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2055 2211 2274 2664 2876 3116 3203
Size: 399 265 156 63 390 212 240 87
Cuts at: 3203 3945 4030 5101 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 390 265 240 212
156 87 85 63
BspEI T'CCGG_A
Cuts at: 3094 5547 3094
Size: 2453 3431
BspHI T'CATG_A
Cuts at: 2676 3773 5503 2676
Size: 1097 1730 3057
Fragments arranged by size:
3057 1730 1097
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2382 3239 844
Size: 1538 857 3489
Fragments arranged by size:
3489 1538 857
BsrBI CCG'CTC
Cuts at: 1394 1823 2637 3771 1394
Size: 429 814 1134 3507
Fragments arranged by size:
3507 1134 814 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2314 2601 4379 4553 65
Size: 1183 1066 287 1778 174 1396
Fragments arranged by size:
1778 1396 1183 1066 287 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2030 2942 4528 4715 1700
Size: 110 220 912 1586 187 2869
Fragments arranged by size:
2869 1586 912 220 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5884
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5884
BssSI C'ACGA_G
Cuts at: 2099 3944 4956 2099
Size: 1845 1012 3027
Fragments arranged by size:
3027 1845 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5884
BstBI TT'CG_AA
Cuts at: 4688 4688
Size: 5884
BstEII G'GTnAC_C
Cuts at: 1184 2173 2467 1184
Size: 989 294 4601
Fragments arranged by size:
4601 989 294
BstXI CCAn_nnnn'nTGG
Cuts at: 2782 2992 2782
Size: 210 5674
BstYI r'GATC_y
Cuts at: 1972 3214 3581 3966 3983 5435 5521 5533 5879
Size: 1242 367 385 17 1452 86 12 346
Cuts at: 5879 1972
Size: 1977
Fragments arranged by size:
1977 1452 1242 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2979 3069 3174 513
Size: 552 939 975 90 105 3223
Fragments arranged by size:
3223 975 939 552 105 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2703 2953 487
Size: 683 56 1477 250 3418
Fragments arranged by size:
3418 1477 683 250 56
BtsI GCAGTG_nn'
Cuts at: 3400 4194 4214 3400
Size: 794 20 5070
Fragments arranged by size:
5070 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5884
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5849
DraI TTT'AAA
Cuts at: 3188 3188
Size: 5884
DraIII CAC_nnn'GTG
Cuts at: 2173 2173
Size: 5884
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4891 809
Size: 507 3575 1802
Fragments arranged by size:
3575 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2319 2655 2902
Size: 54 1425 50 275 18 489 336 247
Cuts at: 2902 2982 2994 3166 3331 4220 5849 8
Size: 80 12 172 165 889 1629 43
Fragments arranged by size:
1629 1425 889 489 336 275 247 172
165 80 54 50 43 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 2655 3166 3331 1537
Size: 275 843 511 165 4090
Fragments arranged by size:
4090 843 511 275 165
EarI CTCTTCn'nnn_
Cuts at: 1353 2259 2289 3814 1353
Size: 906 30 1525 3423
Fragments arranged by size:
3423 1525 906 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2315 3134 4471 4845 4991 387
Size: 1064 138 726 819 1337 374 146 1280
Fragments arranged by size:
1337 1280 1064 819 726 374 146 138
EcoICRI GAG'CTC
Cuts at: 2209 2209
Size: 5884
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2033 2218 2366 2555 2591 2687 3144
Size: 393 401 185 148 189 36 96 457
Cuts at: 3144 3947 4544 5331 5599 1239
Size: 803 597 787 268 1524
Fragments arranged by size:
1524 803 787 597 457 401 393 268
189 185 148 96 36
EcoO109I rG'GnC_Cy
Cuts at: 2104 2872 2104
Size: 768 5116
EcoRI G'AATT_C
Cuts at: 3193 3571 3193
Size: 378 5506
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2248 2248
Size: 5884
FspI TGC'GCA
Cuts at: 2600 4390 2600
Size: 1790 4094
HaeII r_GCGC'y
Cuts at: 1678 2012 2040 2097 5031 1678
Size: 334 28 57 2934 2531
Fragments arranged by size:
2934 2531 334 57 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2809 2841 4525 4557 4599
Size: 32 135 32 1237 32 1684 32 42
Cuts at: 4599 4631 1373
Size: 32 2626
Fragments arranged by size:
2626 1684 1237 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2845 3228 3474 669
Size: 988 281 907 383 246 3079
Fragments arranged by size:
3079 988 907 383 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5884
HpaI GTT'AAC
Cuts at: 3474 3474
Size: 5884
KasI G'GCGC_C
Cuts at: 2093 2093
Size: 5884
KpnI G_GTAC'C
Cuts at: 3209 3209
Size: 5884
MfeI C'AATT_G
Cuts at: 3483 3483
Size: 5884
MluI A'CGCG_T
Cuts at: 5760 5760
Size: 5884
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4998 5182 1305
Size: 3693 184 2007
Fragments arranged by size:
3693 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2321 2904 2996 10
Size: 54 1425 343 489 583 92 2898
Fragments arranged by size:
2898 1425 583 489 343 92 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2244 2544 2759 2774 2816 3033 3842
Size: 648 1078 300 215 15 42 217 809
Cuts at: 3842 4201 4360 518
Size: 359 159 2042
Fragments arranged by size:
2042 1078 809 648 359 300 217 215
159 42 15
MspA1I CmG'CkG
Cuts at: 1606 2782 3000 3300 3977 5125 5370 1606
Size: 1176 218 300 677 1148 245 2120
Fragments arranged by size:
2120 1176 1148 677 300 245 218
NaeI GCC'GGC
Cuts at: 2032 2032
Size: 5884
NarI GG'CG_CC
Cuts at: 2094 2094
Size: 5884
NcoI C'CATG_G
Cuts at: 513 1065 2004 3069 513
Size: 552 939 1065 3328
Fragments arranged by size:
3328 1065 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5884
NgoMIV G'CCGG_C
Cuts at: 2030 2030
Size: 5884
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5884
NotI GC'GGCC_GC
Cuts at: 3331 3331
Size: 5884
NspI r_CATG'y
Cuts at: 1132 1229 2222 2247 3242 4787 1132
Size: 97 993 25 995 1545 2229
Fragments arranged by size:
2229 1545 995 993 97 25
PasI CC'CwG_GG
Cuts at: 1348 2079 2186 1348
Size: 731 107 5046
Fragments arranged by size:
5046 731 107
PciI A'CATG_T
Cuts at: 4783 4783
Size: 5884
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2192 1108
Size: 1084 4800
PfoI T'CCnGG_A
Cuts at: 2502 3132 2502
Size: 630 5254
PmeI GTTT'AAAC
Cuts at: 3188 3188
Size: 5884
PmlI CAC'GTG
Cuts at: 2275 2275
Size: 5884
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3953 3985 5466 5498 3953
Size: 32 1481 32 4339
Fragments arranged by size:
4339 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2104 2104
Size: 5884
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5884
PsiI TTA'TAA
Cuts at: 3454 3454
Size: 5884
PspOMI G'GGCC_C
Cuts at: 2872 2872
Size: 5884
PspXI vC'TCGA_Gb
Cuts at: 2110 2110
Size: 5884
PstI C_TGCA'G
Cuts at: 830 2714 3236 830
Size: 1884 522 3478
Fragments arranged by size:
3478 1884 522
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5884
PvuII CAG'CTG
Cuts at: 2782 3000 2782
Size: 218 5666
SacI G_AGCT'C
Cuts at: 2211 2211
Size: 5884
SalI G'TCGA_C
Cuts at: 1936 3226 1936
Size: 1290 4594
SbfI CC_TGCA'GG
Cuts at: 3236 3236
Size: 5884
ScaI AGT'ACT
Cuts at: 4132 5844 4132
Size: 1712 4172
SfcI C'TryA_G
Cuts at: 826 1045 2710 3232 4367 5048 5239 826
Size: 219 1665 522 1135 681 191 1471
Fragments arranged by size:
1665 1471 1135 681 522 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3179 3179
Size: 5884
SfoI GGC'GCC
Cuts at: 2095 2095
Size: 5884
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5884
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5884
SmaI CCC'GGG
Cuts at: 1872 3211 1872
Size: 1339 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2110 3020 3988 4889 5151 820
Size: 197 926 167 910 968 901 262 1553
Fragments arranged by size:
1553 968 926 910 901 262 197 167
SnaBI TAC'GTA
Cuts at: 493 3675 493
Size: 3182 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5884
SphI G_CATG'C
Cuts at: 1132 3242 1132
Size: 2110 3774
SspI AAT'ATT
Cuts at: 5 52 3808 5
Size: 47 3756 2081
Fragments arranged by size:
3756 2081 47
StuI AGG'CCT
Cuts at: 1517 2880 1517
Size: 1363 4521
StyI C'CwwG_G
Cuts at: 513 1065 2004 2583 3069 3323 513
Size: 552 939 579 486 254 3074
Fragments arranged by size:
3074 939 579 552 486 254
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3911 3928 4081 4266 4711 325
Size: 1255 2331 17 153 185 445 1498
Fragments arranged by size:
2331 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2523 4130 5842
Size: 274 80 33 51 1443 546 1607 1712
Cuts at: 5842 96
Size: 138
Fragments arranged by size:
1712 1607 1443 546 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2264 2296 5844 5876 2264
Size: 32 3548 32 2272
Fragments arranged by size:
3548 2272 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5884
XbaI T'CTAG_A
Cuts at: 3220 3220
Size: 5884
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2779 2779
Size: 5884
XhoI C'TCGA_G
Cuts at: 1943 2110 1943
Size: 167 5717
XmaI C'CCGG_G
Cuts at: 1870 3209 1870
Size: 1339 4545
XmnI GAAnn'nnTTC
Cuts at: 4013 4013
Size: 5884
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3139 3692 276
Size: 53 83 186 936 1605 553 2468
Fragments arranged by size:
2468 1605 936 553 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FseI
FspAI NruI NsiI PacI PsrI RsrII SacII SanDI
SapI SexAI SrfI SwaI