Restriction map for
pFN21AE0418
(Circular) (Six-base) MAPSORT of: pFN21AE0418.seq Check: 1014 from: 1 to: 5974
pFN21AE0418 5974 bp
With 182 enzymes: SgfI *
October 20, 2009 10:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3784 278
Size: 53 83 186 936 2248 2468
Fragments arranged by size:
2468 2248 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3317 1937
Size: 1380 4594
Acc65I G'GTAC_C
Cuts at: 3295 3295
Size: 5974
AclI AA'CG_TT
Cuts at: 1563 4101 4474 1563
Size: 2538 373 3063
Fragments arranged by size:
3063 2538 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2592 2732 2902 2926 4037 5421 5689 1632
Size: 960 140 170 24 1111 1384 268 1917
Fragments arranged by size:
1917 1384 1111 960 268 170 140 24
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5777
AflIII A'CryG_T
Cuts at: 1203 4873 5850 1203
Size: 3670 977 1327
Fragments arranged by size:
3670 1327 977
AgeI A'CCGG_T
Cuts at: 4805 4805
Size: 5974
AhdI GACnn_n'nnGTC
Cuts at: 4703 4703
Size: 5974
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5974
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5974
AlwNI CAG_nnn'CTG
Cuts at: 3153 3717 5289 3153
Size: 564 1572 3838
Fragments arranged by size:
3838 1572 564
ApaI G_GGCC'C
Cuts at: 2071 2754 2071
Size: 683 5291
ApaLI G'TGCA_C
Cuts at: 4031 5187 4031
Size: 1156 4818
ApoI r'AATT_y
Cuts at: 1493 2309 3283 3509 3661 5770 1493
Size: 816 974 226 152 2109 1697
Fragments arranged by size:
2109 1697 974 816 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4528 160
Size: 872 3496 1606
Fragments arranged by size:
3496 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5974
AvaI C'yCGr_G
Cuts at: 1870 1943 2303 2500 3299 1870
Size: 73 360 197 799 4545
Fragments arranged by size:
4545 799 360 197 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2966 2999 2966
Size: 33 5941
BamHI G'GATC_C
Cuts at: 2226 3304 3671 2226
Size: 1078 367 4529
Fragments arranged by size:
4529 1078 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3295 4750 618
Size: 325 217 627 1508 1455 1842
Fragments arranged by size:
1842 1508 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2071 2754 2810 3293 1391
Size: 680 683 56 483 4072
Fragments arranged by size:
4072 683 680 483 56
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2580 2693 928
Size: 971 681 113 4209
Fragments arranged by size:
4209 971 681 113
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2004 2038 2105 2139 4165 4199 1903
Size: 34 67 34 67 34 2026 34 3678
Fragments arranged by size:
3678 2026 67 67 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3866 5076 3866
Size: 1210 4764
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5974
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2672 4585 136
Size: 107 122 71 631 605 1000 1913 1525
Fragments arranged by size:
1913 1525 1000 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5969 5969
Size: 5974
BlpI GC'TnA_GC
Cuts at: 3391 3391
Size: 5974
Bme1580I G_kGCm'C
Cuts at: 1790 2071 2304 2754 4035 5191 1790
Size: 281 233 450 1281 1156 2573
Fragments arranged by size:
2573 1281 1156 450 281 233
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5974
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3705 4663 442
Size: 416 53 83 399 24 2288 958 1753
Fragments arranged by size:
2288 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5974
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2829 3154 2829
Size: 325 5649
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2876 2992 3206 4634 1239
Size: 1637 116 214 1428 2579
Fragments arranged by size:
2579 1637 1428 214 116
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2498 2819 4099 4964 5262 2498
Size: 321 1280 865 298 3210
Fragments arranged by size:
3210 1280 865 321 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2273 2323 4637 882
Size: 617 774 50 2314 2219
Fragments arranged by size:
2314 2219 774 617 50
BsaAI yAC'GTr
Cuts at: 493 1204 2299 3765 493
Size: 711 1095 1466 2702
Fragments arranged by size:
2702 1466 1095 711
BsaBI GATnn'nnATC
Cuts at: 5968 5968
Size: 5974
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2202 2830 3781 4163
Size: 53 83 186 936 669 628 951 382
Cuts at: 4163 4798 275
Size: 635 1451
Fragments arranged by size:
1451 951 936 669 635 628 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4407 4805 5079 5226 5637 4407
Size: 398 274 147 411 4744
Fragments arranged by size:
4744 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2127 2157 1178
Size: 30 165 30 724 30 4995
Fragments arranged by size:
4995 724 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2480 2802 1186
Size: 1294 322 4358
Fragments arranged by size:
4358 1294 322
BseYI C'CCAG_C
Cuts at: 2447 2538 5177 5673 2447
Size: 91 2639 496 2748
Fragments arranged by size:
2748 2639 496 91
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5401
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2035 3424 4185 5213 1540
Size: 275 186 34 1389 761 1028 2301
Fragments arranged by size:
2301 1389 1028 761 275 186 34
BsiHKAI G_wGCw'C
Cuts at: 4035 4120 5191 4035
Size: 85 1071 4818
Fragments arranged by size:
4818 1071 85
BsmI GAATG_Cn'
Cuts at: 3100 3485 3578 3100
Size: 385 93 5496
Fragments arranged by size:
5496 385 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2837 2837
Size: 5974
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2071 2304 2754 2810 3293 4035 4120
Size: 399 281 233 450 56 483 742 85
Cuts at: 4120 5191 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 483 450 399 281 233
85 56
BspEI T'CCGG_A
Cuts at: 5637 5637
Size: 5974
BspHI T'CATG_A
Cuts at: 3863 5593 3863
Size: 1730 4244
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3329 844
Size: 2485 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2138 3861 1394
Size: 429 315 1723 3507
Fragments arranged by size:
3507 1723 429 315
BsrDI GCAATG_nn'
Cuts at: 65 1248 4469 4643 65
Size: 1183 3221 174 1396
Fragments arranged by size:
3221 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3019 4618 4805 1700
Size: 110 1209 1599 187 2869
Fragments arranged by size:
2869 1599 1209 187 110
BsrGI T'GTAC_A
Cuts at: 96 3078 96
Size: 2982 2992
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5974
BssSI C'ACGA_G
Cuts at: 2402 2668 4034 5046 2402
Size: 266 1366 1012 3330
Fragments arranged by size:
3330 1366 1012 266
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5974
BstBI TT'CG_AA
Cuts at: 4778 4778
Size: 5974
BstEII G'GTnAC_C
Cuts at: 1184 2143 1184
Size: 959 5015
BstXI CCAn_nnnn'nTGG
Cuts at: 2164 2164
Size: 5974
BstYI r'GATC_y
Cuts at: 1972 2226 2991 3304 3671 4056 4073 5525 5611
Size: 254 765 313 367 385 17 1452 86
Cuts at: 5611 5623 5969 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 765 385 367 346 313 254
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2130 2580 2883 3226 3270 513
Size: 552 939 126 450 303 343 44 3217
Fragments arranged by size:
3217 939 552 450 343 303 126 44
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5235
Fragments arranged by size:
5235 683 56
BtsI GCAGTG_nn'
Cuts at: 2022 2331 3490 4284 4304 2022
Size: 309 1159 794 20 3692
Fragments arranged by size:
3692 1159 794 309 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5974
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5939
DraI TTT'AAA
Cuts at: 3278 3278
Size: 5974
DraIII CAC_nnn'GTG
Cuts at: 2878 2878
Size: 5974
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2417 2582 4981 809
Size: 507 1101 165 2399 1802
Fragments arranged by size:
2399 1802 1101 507 165
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2032 2082 2154
Size: 54 1425 50 275 18 202 50 72
Cuts at: 2154 2166 2293 2364 2886 3421 4310 5939 8
Size: 12 127 71 522 535 889 1629 43
Fragments arranged by size:
1629 1425 889 535 522 275 202 127
72 71 54 50 50 43 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 2032 3421 1537
Size: 275 220 1389 4090
Fragments arranged by size:
4090 1389 275 220
EarI CTCTTCn'nnn_
Cuts at: 1353 2643 3904 1353
Size: 1290 1261 3423
Fragments arranged by size:
3423 1290 1261
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2654 2692 4561 4935 5081 387
Size: 1064 138 1065 38 1869 374 146 1280
Fragments arranged by size:
1869 1280 1065 1064 374 146 138 38
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2592 2732 2876 2902 2926 2992 3206
Size: 393 960 140 144 26 24 66 214
Cuts at: 3206 4037 4634 5421 5689 1239
Size: 831 597 787 268 1524
Fragments arranged by size:
1524 960 831 787 597 393 268 214
144 140 66 26 24
EcoO109I rG'GnC_Cy
Cuts at: 2068 2410 2556 2610 2750 2923 2068
Size: 342 146 54 140 173 5119
Fragments arranged by size:
5119 342 173 146 140 54
EcoRI G'AATT_C
Cuts at: 2309 3283 3661 2309
Size: 974 378 4622
Fragments arranged by size:
4622 974 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2539 2539
Size: 5974
FspI TGC'GCA
Cuts at: 4480 4480
Size: 5974
HaeII r_GCGC'y
Cuts at: 1678 5121 1678
Size: 3443 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3179 3211 4615 4647 4689
Size: 32 135 32 1607 32 1404 32 42
Cuts at: 4689 4721 1373
Size: 32 2626
Fragments arranged by size:
2626 1607 1404 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3318 3564 669
Size: 988 281 1380 246 3079
Fragments arranged by size:
3079 1380 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5974
HpaI GTT'AAC
Cuts at: 3564 3564
Size: 5974
KpnI G_GTAC'C
Cuts at: 3299 3299
Size: 5974
MfeI C'AATT_G
Cuts at: 3573 3573
Size: 5974
MluI A'CGCG_T
Cuts at: 5850 5850
Size: 5974
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5088 5272 1305
Size: 3783 184 2007
Fragments arranged by size:
3783 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2168 2366 2888 10
Size: 54 1425 343 336 198 522 3096
Fragments arranged by size:
3096 1425 522 343 336 198 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2741 3059 3080 3932 4291 4450 518
Size: 648 1575 318 21 852 359 159 2042
Fragments arranged by size:
2042 1575 852 648 359 318 159 21
MspA1I CmG'CkG
Cuts at: 1606 2198 2239 2339 2538 2854 3390 4067 5215
Size: 592 41 100 199 316 536 677 1148
Cuts at: 5215 5460 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 592 536 316 245 199
100 41
NaeI GCC'GGC
Cuts at: 3021 3021
Size: 5974
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4483
Fragments arranged by size:
4483 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5974
NgoMIV G'CCGG_C
Cuts at: 3019 3019
Size: 5974
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5974
NotI GC'GGCC_GC
Cuts at: 3421 3421
Size: 5974
NspI r_CATG'y
Cuts at: 1132 1229 3332 4877 1132
Size: 97 2103 1545 2229
Fragments arranged by size:
2229 2103 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2064 2927 1348
Size: 716 863 4395
Fragments arranged by size:
4395 863 716
PciI A'CATG_T
Cuts at: 4873 4873
Size: 5974
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2362 2746 3140 1108
Size: 1254 384 394 3942
Fragments arranged by size:
3942 1254 394 384
PmeI GTTT'AAAC
Cuts at: 3278 3278
Size: 5974
PmlI CAC'GTG
Cuts at: 2299 2299
Size: 5974
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2091 2123 4043 4075 5556 5588 2091
Size: 32 1920 32 1481 32 2477
Fragments arranged by size:
2477 1920 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2410 2556 2610 2923 2410
Size: 146 54 313 5461
Fragments arranged by size:
5461 313 146 54
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5974
PsiI TTA'TAA
Cuts at: 2592 3544 2592
Size: 952 5022
PspOMI G'GGCC_C
Cuts at: 2067 2750 2067
Size: 683 5291
PstI C_TGCA'G
Cuts at: 830 2721 3326 830
Size: 1891 605 3478
Fragments arranged by size:
3478 1891 605
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5974
PvuII CAG'CTG
Cuts at: 2538 2538
Size: 5974
SalI G'TCGA_C
Cuts at: 1936 3316 1936
Size: 1380 4594
SanDI GG'GwC_CC
Cuts at: 2556 2923 2556
Size: 367 5607
SbfI CC_TGCA'GG
Cuts at: 3326 3326
Size: 5974
ScaI AGT'ACT
Cuts at: 4222 5934 4222
Size: 1712 4262
SfcI C'TryA_G
Cuts at: 826 1045 2533 2717 3322 4457 5138 5329 826
Size: 219 1488 184 605 1135 681 191 1471
Fragments arranged by size:
1488 1471 1135 681 605 219 191 184
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5974
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5974
SmaI CCC'GGG
Cuts at: 1872 2305 3301 1872
Size: 433 996 4545
Fragments arranged by size:
4545 996 433
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2513 2834 4078 4979 5241 820
Size: 197 926 570 321 1244 901 262 1553
Fragments arranged by size:
1553 1244 926 901 570 321 262 197
SnaBI TAC'GTA
Cuts at: 493 3765 493
Size: 3272 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5974
SphI G_CATG'C
Cuts at: 1132 3332 1132
Size: 2200 3774
SspI AAT'ATT
Cuts at: 5 52 3898 5
Size: 47 3846 2081
Fragments arranged by size:
3846 2081 47
StuI AGG'CCT
Cuts at: 1517 2091 1517
Size: 574 5400
StyI C'CwwG_G
Cuts at: 513 1065 2004 2169 3413 513
Size: 552 939 165 1244 3074
Fragments arranged by size:
3074 1244 939 552 165
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2438 4001 4018 4171 4356 4801 325
Size: 1255 858 1563 17 153 185 445 1498
Fragments arranged by size:
1563 1498 1255 858 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3078 4220 5932
Size: 274 80 33 51 1443 1101 1142 1712
Cuts at: 5932 96
Size: 138
Fragments arranged by size:
1712 1443 1142 1101 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2127 2159 2852 2864 2884 2896 5934 5966 2127
Size: 32 693 12 20 12 3038 32 2135
Fragments arranged by size:
3038 2135 693 32 32 20 12 12
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5974
XbaI T'CTAG_A
Cuts at: 3310 3310
Size: 5974
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2137 2374 3167 2137
Size: 237 793 4944
Fragments arranged by size:
4944 793 237
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5974
XmaI C'CCGG_G
Cuts at: 1870 2303 3299 1870
Size: 433 996 4545
Fragments arranged by size:
4545 996 433
XmnI GAAnn'nnTTC
Cuts at: 2727 3213 4103 2727
Size: 486 890 4598
Fragments arranged by size:
4598 890 486
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3782 276
Size: 53 83 186 936 2248 2468
Fragments arranged by size:
2468 2248 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI
Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI
FspAI KasI NarI NruI NsiI PacI PfoI PspXI
PsrI RsrII SacI SacII SapI SexAI SfiI SfoI
SrfI SwaI