Restriction map for
pFN21AE0409
(Circular) (Six-base) MAPSORT of: pFN21AE0409.seq Check: 3481 from: 1 to: 5290
pFN21AE0409 5290 bp
With 182 enzymes: SgfI *
October 20, 2009 10:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3100 278
Size: 53 83 186 936 1564 2468
Fragments arranged by size:
2468 1564 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2197 2633 1937
Size: 260 436 4594
Fragments arranged by size:
4594 436 260
Acc65I G'GTAC_C
Cuts at: 2611 2611
Size: 5290
AclI AA'CG_TT
Cuts at: 1563 3417 3790 1563
Size: 1854 373 3063
Fragments arranged by size:
3063 1854 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2148 2478 3353 4737 5005 1632
Size: 516 330 875 1384 268 1917
Fragments arranged by size:
1917 1384 875 516 330 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5093
AflIII A'CryG_T
Cuts at: 1203 4189 5166 1203
Size: 2986 977 1327
Fragments arranged by size:
2986 1327 977
AgeI A'CCGG_T
Cuts at: 4121 4121
Size: 5290
AhdI GACnn_n'nnGTC
Cuts at: 4019 4019
Size: 5290
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5290
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5290
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2150 2182 2150
Size: 32 5258
AlwNI CAG_nnn'CTG
Cuts at: 2363 3033 4605 2363
Size: 670 1572 3048
Fragments arranged by size:
3048 1572 670
ApaI G_GGCC'C
Cuts at: 2239 2239
Size: 5290
ApaLI G'TGCA_C
Cuts at: 3347 4503 3347
Size: 1156 4134
ApoI r'AATT_y
Cuts at: 1493 2112 2599 2825 2977 5086 1493
Size: 619 487 226 152 2109 1697
Fragments arranged by size:
2109 1697 619 487 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3844 160
Size: 872 2812 1606
Fragments arranged by size:
2812 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5290
AvaI C'yCGr_G
Cuts at: 1870 1943 2615 1870
Size: 73 672 4545
Fragments arranged by size:
4545 672 73
BamHI G'GATC_C
Cuts at: 2620 2987 2620
Size: 367 4923
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2578 2611 4066 618
Size: 325 217 627 791 33 1455 1842
Fragments arranged by size:
1842 1455 791 627 325 217 33
BanII G_rGCy'C
Cuts at: 1391 2031 2239 2609 1391
Size: 640 208 370 4072
Fragments arranged by size:
4072 640 370 208
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2521 928
Size: 971 622 3697
Fragments arranged by size:
3697 971 622
BbvCI CC'TCA_GC
Cuts at: 2348 2348
Size: 5290
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3481 3515 1903
Size: 34 1544 34 3678
Fragments arranged by size:
3678 1544 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3182 4392 3182
Size: 1210 4080
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5290
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3901 136
Size: 107 122 71 631 605 2229 1525
Fragments arranged by size:
2229 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5285 5285
Size: 5290
BlpI GC'TnA_GC
Cuts at: 2078 2707 2078
Size: 629 4661
Bme1580I G_kGCm'C
Cuts at: 1790 2239 3351 4507 1790
Size: 449 1112 1156 2573
Fragments arranged by size:
2573 1156 1112 449
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5290
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2233 3021 3979
Size: 416 53 83 399 24 816 788 958
Cuts at: 3979 442
Size: 1753
Fragments arranged by size:
1753 958 816 788 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5290
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2047 2047
Size: 5290
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3950 1239
Size: 2711 2579
Bpu10I CC'TnA_GC
Cuts at: 2348 2348
Size: 5290
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2288 2558 3415 4280 4578 2288
Size: 270 857 865 298 3000
Fragments arranged by size:
3000 865 857 298 270
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3953 882
Size: 617 2454 2219
Fragments arranged by size:
2454 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3081 493
Size: 711 1877 2702
Fragments arranged by size:
2702 1877 711
BsaBI GATnn'nnATC
Cuts at: 5284 5284
Size: 5290
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3097 3479 4114 275
Size: 53 83 186 936 1564 382 635 1451
Fragments arranged by size:
1564 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3723 4121 4395 4542 4953 3723
Size: 398 274 147 411 4060
Fragments arranged by size:
4060 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5065
Fragments arranged by size:
5065 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2039 1186
Size: 853 4437
BseYI C'CCAG_C
Cuts at: 4493 4989 4493
Size: 496 4794
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2039 1163
Size: 573 303 4414
Fragments arranged by size:
4414 573 303
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2740 3501 4529 1540
Size: 275 186 739 761 1028 2301
Fragments arranged by size:
2301 1028 761 739 275 186
BsiHKAI G_wGCw'C
Cuts at: 2031 3351 3436 4507 2031
Size: 1320 85 1071 2814
Fragments arranged by size:
2814 1320 1071 85
BsmI GAATG_Cn'
Cuts at: 2801 2894 2801
Size: 93 5197
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2031 2239 2609 3351 3436 4507 1391
Size: 399 241 208 370 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 370 241 208 85
BspEI T'CCGG_A
Cuts at: 4953 4953
Size: 5290
BspHI T'CATG_A
Cuts at: 2305 3179 4909 2305
Size: 874 1730 2686
Fragments arranged by size:
2686 1730 874
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2645 844
Size: 1801 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2085 3177 1394
Size: 429 262 1092 3507
Fragments arranged by size:
3507 1092 429 262
BsrDI GCAATG_nn'
Cuts at: 65 1248 3785 3959 65
Size: 1183 2537 174 1396
Fragments arranged by size:
2537 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3934 4121 1700
Size: 110 2124 187 2869
Fragments arranged by size:
2869 2124 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5290
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5290
BssSI C'ACGA_G
Cuts at: 2063 3350 4362 2063
Size: 1287 1012 2991
Fragments arranged by size:
2991 1287 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5290
BstBI TT'CG_AA
Cuts at: 4094 4094
Size: 5290
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5290
BstYI r'GATC_y
Cuts at: 1972 2620 2987 3372 3389 4841 4927 4939 5285
Size: 648 367 385 17 1452 86 12 346
Cuts at: 5285 1972
Size: 1977
Fragments arranged by size:
1977 1452 648 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2406 513
Size: 552 939 402 3397
Fragments arranged by size:
3397 939 552 402
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4551
Fragments arranged by size:
4551 683 56
BtsI GCAGTG_nn'
Cuts at: 2374 2380 2806 3600 3620 2374
Size: 6 426 794 20 4044
Fragments arranged by size:
4044 794 426 20 6
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5290
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5255
DraI TTT'AAA
Cuts at: 2594 2594
Size: 5290
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4297 809
Size: 507 2981 1802
Fragments arranged by size:
2981 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2040 2737 3626
Size: 54 1425 50 275 18 210 697 889
Cuts at: 3626 5255 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 697 275 210 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2737 1537
Size: 275 925 4090
Fragments arranged by size:
4090 925 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3220 1353
Size: 1867 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 3877 4251 4397 387
Size: 1064 138 435 1853 374 146 1280
Fragments arranged by size:
1853 1280 1064 435 374 146 138
EcoICRI GAG'CTC
Cuts at: 2029 2029
Size: 5290
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2148 2478 3353 3950 4737 5005 1239
Size: 393 516 330 875 597 787 268 1524
Fragments arranged by size:
1524 875 787 597 516 393 330 268
EcoO109I rG'GnC_Cy
Cuts at: 2058 2235 2058
Size: 177 5113
EcoRI G'AATT_C
Cuts at: 2112 2599 2977 2112
Size: 487 378 4425
Fragments arranged by size:
4425 487 378
FspI TGC'GCA
Cuts at: 3796 3796
Size: 5290
HaeII r_GCGC'y
Cuts at: 1678 4437 1678
Size: 2759 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3931 3963 4005 4037 1373
Size: 32 135 32 2359 32 42 32 2626
Fragments arranged by size:
2626 2359 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2229 2634 2880 669
Size: 988 281 291 405 246 3079
Fragments arranged by size:
3079 988 405 291 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5290
HpaI GTT'AAC
Cuts at: 2880 2880
Size: 5290
KpnI G_GTAC'C
Cuts at: 2615 2615
Size: 5290
MfeI C'AATT_G
Cuts at: 2889 2889
Size: 5290
MluI A'CGCG_T
Cuts at: 5166 5166
Size: 5290
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2271 4404 4588 1305
Size: 966 2133 184 2007
Fragments arranged by size:
2133 2007 966 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3468
Fragments arranged by size:
3468 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2405 3248 3607 3766 518
Size: 648 1239 843 359 159 2042
Fragments arranged by size:
2042 1239 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2706 3383 4531 4776 1606
Size: 1100 677 1148 245 2120
Fragments arranged by size:
2120 1148 1100 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2406 513
Size: 552 939 402 3397
Fragments arranged by size:
3397 939 552 402
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5290
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5290
NotI GC'GGCC_GC
Cuts at: 2737 2737
Size: 5290
NspI r_CATG'y
Cuts at: 1132 1229 2648 4193 1132
Size: 97 1419 1545 2229
Fragments arranged by size:
2229 1545 1419 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5290
PciI A'CATG_T
Cuts at: 4189 4189
Size: 5290
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2363 1108
Size: 1255 4035
PmeI GTTT'AAAC
Cuts at: 2594 2594
Size: 5290
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3359 3391 4872 4904 3359
Size: 32 1481 32 3745
Fragments arranged by size:
3745 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5290
PsiI TTA'TAA
Cuts at: 2860 2860
Size: 5290
PspOMI G'GGCC_C
Cuts at: 2235 2235
Size: 5290
PstI C_TGCA'G
Cuts at: 830 2057 2642 830
Size: 1227 585 3478
Fragments arranged by size:
3478 1227 585
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5290
SacI G_AGCT'C
Cuts at: 2031 2031
Size: 5290
SalI G'TCGA_C
Cuts at: 1936 2632 1936
Size: 696 4594
SbfI CC_TGCA'GG
Cuts at: 2642 2642
Size: 5290
ScaI AGT'ACT
Cuts at: 3538 5250 3538
Size: 1712 3578
SexAI A'CCwGG_T
Cuts at: 2200 2200
Size: 5290
SfcI C'TryA_G
Cuts at: 826 1045 2053 2552 2638 3773 4454 4645 826
Size: 219 1008 499 86 1135 681 191 1471
Fragments arranged by size:
1471 1135 1008 681 499 219 191 86
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5290
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5290
SmaI CCC'GGG
Cuts at: 1872 2617 1872
Size: 745 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2267 2573 3394 4295 4557 820
Size: 197 926 324 306 821 901 262 1553
Fragments arranged by size:
1553 926 901 821 324 306 262 197
SnaBI TAC'GTA
Cuts at: 493 3081 493
Size: 2588 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5290
SphI G_CATG'C
Cuts at: 1132 2648 1132
Size: 1516 3774
SspI AAT'ATT
Cuts at: 5 52 3214 5
Size: 47 3162 2081
Fragments arranged by size:
3162 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5290
StyI C'CwwG_G
Cuts at: 513 1065 2004 2406 2729 513
Size: 552 939 402 323 3074
Fragments arranged by size:
3074 939 552 402 323
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2023 3317 3334 3487 3672 4117 325
Size: 1255 443 1294 17 153 185 445 1498
Fragments arranged by size:
1498 1294 1255 445 443 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2384 3536 5248
Size: 274 80 33 51 1443 407 1152 1712
Cuts at: 5248 96
Size: 138
Fragments arranged by size:
1712 1443 1152 407 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5250 5282 5250
Size: 32 5258
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5290
XbaI T'CTAG_A
Cuts at: 2626 2626
Size: 5290
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2360 2360
Size: 5290
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5290
XmaI C'CCGG_G
Cuts at: 1870 2615 1870
Size: 745 4545
XmnI GAAnn'nnTTC
Cuts at: 3419 3419
Size: 5290
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3098 276
Size: 53 83 186 936 1564 2468
Fragments arranged by size:
2468 1564 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI SacI SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII
SacII SanDI SapI SfiI SfoI SrfI SwaI