Restriction map for
pFN21AE0406
(Circular) (Six-base) MAPSORT of: pFN21AE0406.seq Check: 426 from: 1 to: 5728
pFN21AE0406 5728 bp
With 182 enzymes: SgfI *
October 20, 2009 10:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2396 2396
Size: 5728
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3538 278
Size: 53 83 186 936 2002 2468
Fragments arranged by size:
2468 2002 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3071 1937
Size: 1134 4594
Acc65I G'GTAC_C
Cuts at: 3049 3049
Size: 5728
AclI AA'CG_TT
Cuts at: 1563 3855 4228 1563
Size: 2292 373 3063
Fragments arranged by size:
3063 2292 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2345 2365 3791 5175 5443 1632
Size: 713 20 1426 1384 268 1917
Fragments arranged by size:
1917 1426 1384 713 268 20
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5531
AflIII A'CryG_T
Cuts at: 1203 2346 4627 5604 1203
Size: 1143 2281 977 1327
Fragments arranged by size:
2281 1327 1143 977
AgeI A'CCGG_T
Cuts at: 4559 4559
Size: 5728
AhdI GACnn_n'nnGTC
Cuts at: 4457 4457
Size: 5728
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5728
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5728
AlwNI CAG_nnn'CTG
Cuts at: 2481 3471 5043 2481
Size: 990 1572 3166
Fragments arranged by size:
3166 1572 990
ApaI G_GGCC'C
Cuts at: 2391 2391
Size: 5728
ApaLI G'TGCA_C
Cuts at: 3785 4941 3785
Size: 1156 4572
ApoI r'AATT_y
Cuts at: 1493 2276 2485 2582 3037 3263 3415 5524 1493
Size: 783 209 97 455 226 152 2109 1697
Fragments arranged by size:
2109 1697 783 455 226 209 152 97
AseI AT'TA_AT
Cuts at: 160 1032 4282 160
Size: 872 3250 1606
Fragments arranged by size:
3250 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5728
AvaI C'yCGr_G
Cuts at: 1870 1943 3053 1870
Size: 73 1110 4545
Fragments arranged by size:
4545 1110 73
BamHI G'GATC_C
Cuts at: 3058 3425 3058
Size: 367 5361
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2396 3049 4504 618
Size: 325 217 627 609 653 1455 1842
Fragments arranged by size:
1842 1455 653 627 609 325 217
BanII G_rGCy'C
Cuts at: 1391 2391 3047 1391
Size: 1000 656 4072
Fragments arranged by size:
4072 1000 656
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2371 2810 928
Size: 971 472 439 3846
Fragments arranged by size:
3846 971 472 439
BbvCI CC'TCA_GC
Cuts at: 2401 2401
Size: 5728
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3919 3953 1903
Size: 34 1982 34 3678
Fragments arranged by size:
3678 1982 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3620 4830 3620
Size: 1210 4518
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5728
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4339 136
Size: 107 122 71 631 605 337 2330 1525
Fragments arranged by size:
2330 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5723 5723
Size: 5728
BlpI GC'TnA_GC
Cuts at: 3145 3145
Size: 5728
Bme1580I G_kGCm'C
Cuts at: 1790 2391 2454 3789 4945 1790
Size: 601 63 1335 1156 2573
Fragments arranged by size:
2573 1335 1156 601 63
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5728
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2128 3459 4417
Size: 416 53 83 399 24 711 1331 958
Cuts at: 4417 442
Size: 1753
Fragments arranged by size:
1753 1331 958 711 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5728
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2989 2989
Size: 5728
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4388 1239
Size: 3149 2579
Bpu10I CC'TnA_GC
Cuts at: 2401 2401
Size: 5728
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3853 4718 5016 3853
Size: 865 298 4565
Fragments arranged by size:
4565 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2026 4391 882
Size: 617 527 2365 2219
Fragments arranged by size:
2365 2219 617 527
BsaAI yAC'GTr
Cuts at: 493 1204 3519 493
Size: 711 2315 2702
Fragments arranged by size:
2702 2315 711
BsaBI GATnn'nnATC
Cuts at: 2532 5722 2532
Size: 3190 2538
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3535 3917 4552 275
Size: 53 83 186 936 2002 382 635 1451
Fragments arranged by size:
2002 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2590 4161 4559 4833 4980 5391 2590
Size: 1571 398 274 147 411 2927
Fragments arranged by size:
2927 1571 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2112 2142 1178
Size: 30 165 30 709 30 4764
Fragments arranged by size:
4764 709 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2099 1186
Size: 913 4815
BseYI C'CCAG_C
Cuts at: 2255 2418 4931 5427 2255
Size: 163 2513 496 2556
Fragments arranged by size:
2556 2513 496 163
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2265 1163
Size: 573 529 4626
Fragments arranged by size:
4626 573 529
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3178 3939 4967 1540
Size: 275 186 1177 761 1028 2301
Fragments arranged by size:
2301 1177 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3789 3874 4945 3789
Size: 85 1071 4572
Fragments arranged by size:
4572 1071 85
BsmI GAATG_Cn'
Cuts at: 3239 3332 3239
Size: 93 5635
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2391 2454 3047 3789 3874 4945 1391
Size: 399 601 63 593 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 601 593 399 85 63
BspEI T'CCGG_A
Cuts at: 2590 5391 2590
Size: 2801 2927
BspHI T'CATG_A
Cuts at: 2799 3617 5347 2799
Size: 818 1730 3180
Fragments arranged by size:
3180 1730 818
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2396 3083 844
Size: 1552 687 3489
Fragments arranged by size:
3489 1552 687
BsrBI CCG'CTC
Cuts at: 1394 1823 3615 1394
Size: 429 1792 3507
Fragments arranged by size:
3507 1792 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4223 4397 65
Size: 1183 2975 174 1396
Fragments arranged by size:
2975 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4372 4559 1700
Size: 110 2562 187 2869
Fragments arranged by size:
2869 2562 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5728
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5728
BssSI C'ACGA_G
Cuts at: 3788 4800 3788
Size: 1012 4716
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5728
BstBI TT'CG_AA
Cuts at: 4532 4532
Size: 5728
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5728
BstXI CCAn_nnnn'nTGG
Cuts at: 2141 2878 2141
Size: 737 4991
BstYI r'GATC_y
Cuts at: 1972 3058 3425 3810 3827 5279 5365 5377 5723
Size: 1086 367 385 17 1452 86 12 346
Cuts at: 5723 1972
Size: 1977
Fragments arranged by size:
1977 1452 1086 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2982 2982
Size: 5728
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4237
Fragments arranged by size:
4237 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4989
Fragments arranged by size:
4989 683 56
BtsI GCAGTG_nn'
Cuts at: 2452 2692 2718 3244 4038 4058 2452
Size: 240 26 526 794 20 4122
Fragments arranged by size:
4122 794 526 240 26 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5728
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2667 2702 529
Size: 35 2103 35 3555
Fragments arranged by size:
3555 2103 35 35
DraI TTT'AAA
Cuts at: 3032 3032
Size: 5728
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4735 809
Size: 507 3419 1802
Fragments arranged by size:
3419 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2837 3175 4064
Size: 54 1425 50 275 18 1007 338 889
Cuts at: 4064 5693 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1007 889 338 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3175 1537
Size: 275 1363 4090
Fragments arranged by size:
4090 1363 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3658 1353
Size: 2305 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4315 4689 4835 387
Size: 1064 138 2726 374 146 1280
Fragments arranged by size:
2726 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2345 2365 3791 4388 5175 5443 1239
Size: 393 713 20 1426 597 787 268 1524
Fragments arranged by size:
1524 1426 787 713 597 393 268 20
EcoNI CCTnn'n_nnAGG
Cuts at: 2042 2042
Size: 5728
EcoO109I rG'GnC_Cy
Cuts at: 2387 2885 2387
Size: 498 5230
EcoRI G'AATT_C
Cuts at: 2485 3037 3415 2485
Size: 552 378 4798
Fragments arranged by size:
4798 552 378
FspI TGC'GCA
Cuts at: 4234 4234
Size: 5728
HaeII r_GCGC'y
Cuts at: 1678 4875 1678
Size: 3197 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2797 2829 2847 2879 4369
Size: 32 135 32 1225 32 18 32 1490
Cuts at: 4369 4401 4443 4475 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1490 1225 135 42 32 32 32
32 32 32 18
HincII GTy'rAC
Cuts at: 669 1657 1938 3072 3318 669
Size: 988 281 1134 246 3079
Fragments arranged by size:
3079 1134 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5728
HpaI GTT'AAC
Cuts at: 3318 3318
Size: 5728
KpnI G_GTAC'C
Cuts at: 3053 3053
Size: 5728
MfeI C'AATT_G
Cuts at: 3327 3327
Size: 5728
MluI A'CGCG_T
Cuts at: 5604 5604
Size: 5728
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4842 5026 1305
Size: 3537 184 2007
Fragments arranged by size:
3537 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2839 10
Size: 54 1425 343 1007 2899
Fragments arranged by size:
2899 1425 1007 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3686 4045 4204 518
Size: 648 2520 359 159 2042
Fragments arranged by size:
2520 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2330 3144 3821 4969 5214 1606
Size: 724 814 677 1148 245 2120
Fragments arranged by size:
2120 1148 814 724 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4237
Fragments arranged by size:
4237 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5728
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5728
NotI GC'GGCC_GC
Cuts at: 3175 3175
Size: 5728
NspI r_CATG'y
Cuts at: 1132 1229 2350 3086 4631 1132
Size: 97 1121 736 1545 2229
Fragments arranged by size:
2229 1545 1121 736 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5728
PciI A'CATG_T
Cuts at: 2346 4627 2346
Size: 2281 3447
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2846 1108
Size: 1738 3990
PfoI T'CCnGG_A
Cuts at: 2901 2901
Size: 5728
PmeI GTTT'AAAC
Cuts at: 3032 3032
Size: 5728
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3797 3829 5310 5342 3797
Size: 32 1481 32 4183
Fragments arranged by size:
4183 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2885 2885
Size: 5728
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5728
PsiI TTA'TAA
Cuts at: 2321 3298 2321
Size: 977 4751
PspOMI G'GGCC_C
Cuts at: 2387 2387
Size: 5728
PstI C_TGCA'G
Cuts at: 830 2710 3080 830
Size: 1880 370 3478
Fragments arranged by size:
3478 1880 370
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5728
PvuII CAG'CTG
Cuts at: 2330 2330
Size: 5728
SalI G'TCGA_C
Cuts at: 1936 3070 1936
Size: 1134 4594
SbfI CC_TGCA'GG
Cuts at: 3080 3080
Size: 5728
ScaI AGT'ACT
Cuts at: 3976 5688 3976
Size: 1712 4016
SexAI A'CCwGG_T
Cuts at: 2313 2313
Size: 5728
SfcI C'TryA_G
Cuts at: 826 1045 2238 2373 2706 3076 4211 4892 5083
Size: 219 1193 135 333 370 1135 681 191
Cuts at: 5083 826
Size: 1471
Fragments arranged by size:
1471 1193 1135 681 370 333 219 191
135
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5728
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5728
SmaI CCC'GGG
Cuts at: 1872 3055 1872
Size: 1183 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3832 4733 4995 820
Size: 197 926 1889 901 262 1553
Fragments arranged by size:
1889 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3519 493
Size: 3026 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5728
SphI G_CATG'C
Cuts at: 1132 3086 1132
Size: 1954 3774
SspI AAT'ATT
Cuts at: 5 52 3025 3652 5
Size: 47 2973 627 2081
Fragments arranged by size:
2973 2081 627 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5728
StyI C'CwwG_G
Cuts at: 513 1065 2004 3167 513
Size: 552 939 1163 3074
Fragments arranged by size:
3074 1163 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3755 3772 3925 4110 4555 325
Size: 1255 2175 17 153 185 445 1498
Fragments arranged by size:
2175 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3974 5686 96
Size: 274 80 33 51 1443 1997 1712 138
Fragments arranged by size:
1997 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5688 5720 5688
Size: 32 5696
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5728
XbaI T'CTAG_A
Cuts at: 2962 3064 2962
Size: 102 5626
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2215 2767 2215
Size: 552 5176
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5728
XmaI C'CCGG_G
Cuts at: 1870 3053 1870
Size: 1183 4545
XmnI GAAnn'nnTTC
Cuts at: 3857 3857
Size: 5728
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3536 276
Size: 53 83 186 936 2002 2468
Fragments arranged by size:
2468 2002 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspXI PsrI RsrII SacI SacII SanDI SapI SfiI
SfoI SrfI SwaI