Restriction map for
pFN21AE0404
(Circular) (Six-base) MAPSORT of: pFN21AE0404.seq Check: 8189 from: 1 to: 5527
pFN21AE0404 5527 bp
With 182 enzymes: SgfI *
October 20, 2009 10:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2534 3337 278
Size: 53 83 186 936 998 803 2468
Fragments arranged by size:
2468 998 936 803 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2870 1937
Size: 933 4594
Acc65I G'GTAC_C
Cuts at: 2848 2848
Size: 5527
AclI AA'CG_TT
Cuts at: 1563 3654 4027 1563
Size: 2091 373 3063
Fragments arranged by size:
3063 2091 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3590 4974 5242 1632
Size: 1958 1384 268 1917
Fragments arranged by size:
1958 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5330
AflIII A'CryG_T
Cuts at: 1203 2059 4426 5403 1203
Size: 856 2367 977 1327
Fragments arranged by size:
2367 1327 977 856
AgeI A'CCGG_T
Cuts at: 4358 4358
Size: 5527
AhdI GACnn_n'nnGTC
Cuts at: 2494 4256 2494
Size: 1762 3765
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5527
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5527
AlwNI CAG_nnn'CTG
Cuts at: 2620 2701 3270 4842 2620
Size: 81 569 1572 3305
Fragments arranged by size:
3305 1572 569 81
ApaLI G'TGCA_C
Cuts at: 3584 4740 3584
Size: 1156 4371
ApoI r'AATT_y
Cuts at: 1493 2195 2456 2836 3062 3214 5323 1493
Size: 702 261 380 226 152 2109 1697
Fragments arranged by size:
2109 1697 702 380 261 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4081 160
Size: 872 3049 1606
Fragments arranged by size:
3049 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5527
AvaI C'yCGr_G
Cuts at: 1870 1943 2852 1870
Size: 73 909 4545
Fragments arranged by size:
4545 909 73
BamHI G'GATC_C
Cuts at: 2857 3224 2857
Size: 367 5160
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2848 4303 618
Size: 325 217 627 1061 1455 1842
Fragments arranged by size:
1842 1455 1061 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2352 2846 1391
Size: 961 494 4072
Fragments arranged by size:
4072 961 494
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4556
BbvCI CC'TCA_GC
Cuts at: 2684 2684
Size: 5527
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3718 3752 1903
Size: 34 50 34 1697 34 3678
Fragments arranged by size:
3678 1697 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3419 4629 3419
Size: 1210 4317
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5527
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4138 136
Size: 107 122 71 631 605 2466 1525
Fragments arranged by size:
2466 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5522 5522
Size: 5527
BlpI GC'TnA_GC
Cuts at: 2052 2944 2052
Size: 892 4635
Bme1580I G_kGCm'C
Cuts at: 1790 3588 4744 1790
Size: 1798 1156 2573
Fragments arranged by size:
2573 1798 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5527
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3258 4216 442
Size: 416 53 83 399 24 1841 958 1753
Fragments arranged by size:
1841 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5527
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2272 2366 2606 4187 1239
Size: 1033 94 240 1581 2579
Fragments arranged by size:
2579 1581 1033 240 94
Bpu10I CC'TnA_GC
Cuts at: 2684 2684
Size: 5527
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3652 4517 4815 3652
Size: 865 298 4364
Fragments arranged by size:
4364 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4190 882
Size: 617 2691 2219
Fragments arranged by size:
2691 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3318 493
Size: 711 2114 2702
Fragments arranged by size:
2702 2114 711
BsaBI GATnn'nnATC
Cuts at: 5521 5521
Size: 5527
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2531 2600 3334 3716
Size: 53 83 186 936 998 69 734 382
Cuts at: 3716 4351 275
Size: 635 1451
Fragments arranged by size:
1451 998 936 734 635 382 186 83
69 53
BsaWI w'CCGG_w
Cuts at: 2014 2032 3960 4358 4632 4779 5190 2014
Size: 18 1928 398 274 147 411 2351
Fragments arranged by size:
2351 1928 411 398 274 147 18
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5302
Fragments arranged by size:
5302 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5527
BseYI C'CCAG_C
Cuts at: 2662 2797 4730 5226 2662
Size: 135 1933 496 2963
Fragments arranged by size:
2963 1933 496 135
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4954
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2977 3738 4766 1540
Size: 275 186 976 761 1028 2301
Fragments arranged by size:
2301 1028 976 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2352 2379 3588 3673 4744 2352
Size: 27 1209 85 1071 3135
Fragments arranged by size:
3135 1209 1071 85 27
BsmI GAATG_Cn'
Cuts at: 3038 3131 3038
Size: 93 5434
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2352 2379 2846 3588 3673 4744 1391
Size: 399 562 27 467 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 562 467 399 85 27
BspEI T'CCGG_A
Cuts at: 2014 5190 2014
Size: 3176 2351
BspHI T'CATG_A
Cuts at: 3416 5146 3416
Size: 1730 3797
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2499 2569 2882 844
Size: 1655 70 313 3489
Fragments arranged by size:
3489 1655 313 70
BsrBI CCG'CTC
Cuts at: 1394 1823 3414 1394
Size: 429 1591 3507
Fragments arranged by size:
3507 1591 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2695 2808 4022 4196 65
Size: 1183 1447 113 1214 174 1396
Fragments arranged by size:
1447 1396 1214 1183 174 113
BsrFI r'CCGG_y
Cuts at: 1700 1810 2576 4171 4358 1700
Size: 110 766 1595 187 2869
Fragments arranged by size:
2869 1595 766 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5527
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5527
BssSI C'ACGA_G
Cuts at: 3587 4599 3587
Size: 1012 4515
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5527
BstBI TT'CG_AA
Cuts at: 4331 4331
Size: 5527
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5527
BstYI r'GATC_y
Cuts at: 1972 2436 2782 2857 3224 3609 3626 5078 5164
Size: 464 346 75 367 385 17 1452 86
Cuts at: 5164 5176 5522 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 464 385 367 346 346 86
75 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2391 2740 513
Size: 552 1326 349 3300
Fragments arranged by size:
3300 1326 552 349
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4788
Fragments arranged by size:
4788 683 56
BtsI GCAGTG_nn'
Cuts at: 2091 3043 3837 3857 2091
Size: 952 794 20 3761
Fragments arranged by size:
3761 952 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5527
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5492
DraI TTT'AAA
Cuts at: 2831 2831
Size: 5527
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4534 809
Size: 507 3218 1802
Fragments arranged by size:
3218 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2974 3863 5492
Size: 54 1425 50 275 18 1144 889 1629
Cuts at: 5492 8
Size: 43
Fragments arranged by size:
1629 1425 1144 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2974 1537
Size: 275 1162 4090
Fragments arranged by size:
4090 1162 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2805 3457 1353
Size: 1452 652 3423
Fragments arranged by size:
3423 1452 652
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4114 4488 4634 387
Size: 1064 138 2525 374 146 1280
Fragments arranged by size:
2525 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2350 2350
Size: 5527
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2272 2366 2606 3590 4187 4974 5242
Size: 393 640 94 240 984 597 787 268
Cuts at: 5242 1239
Size: 1524
Fragments arranged by size:
1524 984 787 640 597 393 268 240
94
EcoO109I rG'GnC_Cy
Cuts at: 2488 2680 2488
Size: 192 5335
EcoRI G'AATT_C
Cuts at: 2195 2456 2836 3214 2195
Size: 261 380 378 4508
Fragments arranged by size:
4508 380 378 261
FspI TGC'GCA
Cuts at: 4033 4033
Size: 5527
HaeII r_GCGC'y
Cuts at: 1678 2023 4674 1678
Size: 345 2651 2531
Fragments arranged by size:
2651 2531 345
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2049 2081 2480 2512 4168
Size: 32 135 32 477 32 399 32 1656
Cuts at: 4168 4200 4242 4274 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1656 477 399 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2871 3117 669
Size: 988 281 933 246 3079
Fragments arranged by size:
3079 988 933 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5527
HpaI GTT'AAC
Cuts at: 3117 3117
Size: 5527
KpnI G_GTAC'C
Cuts at: 2852 2852
Size: 5527
MfeI C'AATT_G
Cuts at: 3126 3126
Size: 5527
MluI A'CGCG_T
Cuts at: 5403 5403
Size: 5527
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4641 4825 1305
Size: 3336 184 2007
Fragments arranged by size:
3336 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3705
Fragments arranged by size:
3705 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2072 3485 3844 4003 518
Size: 648 906 1413 359 159 2042
Fragments arranged by size:
2042 1413 906 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2095 2344 2652 2943 3620 4768 5013 1606
Size: 489 249 308 291 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 489 308 291 249 245
NaeI GCC'GGC
Cuts at: 2578 2578
Size: 5527
NcoI C'CATG_G
Cuts at: 513 1065 2391 513
Size: 552 1326 3649
Fragments arranged by size:
3649 1326 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5527
NgoMIV G'CCGG_C
Cuts at: 2576 2576
Size: 5527
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5527
NotI GC'GGCC_GC
Cuts at: 2974 2974
Size: 5527
NspI r_CATG'y
Cuts at: 1132 1229 2063 2885 4430 1132
Size: 97 834 822 1545 2229
Fragments arranged by size:
2229 1545 834 822 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5527
PciI A'CATG_T
Cuts at: 2059 4426 2059
Size: 2367 3160
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2620 1108
Size: 1512 4015
PfoI T'CCnGG_A
Cuts at: 2460 2460
Size: 5527
PmeI GTTT'AAAC
Cuts at: 2831 2831
Size: 5527
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3596 3628 5109 5141 3596
Size: 32 1481 32 3982
Fragments arranged by size:
3982 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2488 2488
Size: 5527
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5527
PsiI TTA'TAA
Cuts at: 3097 3097
Size: 5527
PstI C_TGCA'G
Cuts at: 830 2879 830
Size: 2049 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5527
PvuII CAG'CTG
Cuts at: 2095 2652 2095
Size: 557 4970
SacI G_AGCT'C
Cuts at: 2352 2352
Size: 5527
SalI G'TCGA_C
Cuts at: 1936 2869 1936
Size: 933 4594
SapI GCTCTTCn'nnn_
Cuts at: 2805 2805
Size: 5527
SbfI CC_TGCA'GG
Cuts at: 2879 2879
Size: 5527
ScaI AGT'ACT
Cuts at: 3775 5487 3775
Size: 1712 3815
SfcI C'TryA_G
Cuts at: 826 1045 2875 4010 4691 4882 826
Size: 219 1830 1135 681 191 1471
Fragments arranged by size:
1830 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5527
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5527
SmaI CCC'GGG
Cuts at: 1872 2854 1872
Size: 982 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3631 4532 4794 820
Size: 197 926 1688 901 262 1553
Fragments arranged by size:
1688 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3318 493
Size: 2825 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5527
SphI G_CATG'C
Cuts at: 1132 2885 1132
Size: 1753 3774
SspI AAT'ATT
Cuts at: 5 52 2153 3451 5
Size: 47 2101 1298 2081
Fragments arranged by size:
2101 2081 1298 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5527
StyI C'CwwG_G
Cuts at: 513 1065 2391 2565 2966 513
Size: 552 1326 174 401 3074
Fragments arranged by size:
3074 1326 552 401 174
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3554 3571 3724 3909 4354 325
Size: 1255 1974 17 153 185 445 1498
Fragments arranged by size:
1974 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2646 3773 5485
Size: 274 80 33 51 1443 669 1127 1712
Cuts at: 5485 96
Size: 138
Fragments arranged by size:
1712 1443 1127 669 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2072 2104 2615 2647 5487 5519 2072
Size: 32 511 32 2840 32 2080
Fragments arranged by size:
2840 2080 511 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2533 1316
Size: 1217 4310
XbaI T'CTAG_A
Cuts at: 2863 2863
Size: 5527
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5527
XmaI C'CCGG_G
Cuts at: 1870 2852 1870
Size: 982 4545
XmnI GAAnn'nnTTC
Cuts at: 3656 3656
Size: 5527
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2532 3335 276
Size: 53 83 186 936 998 803 2468
Fragments arranged by size:
2468 998 936 803 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII
SanDI SexAI SfiI SfoI SrfI SwaI XcmI