Restriction map for
pFN21AE0403
(Circular) (Six-base) MAPSORT of: pFN21AE0403.seq Check: 6258 from: 1 to: 5344
pFN21AE0403 5344 bp
With 182 enzymes: SgfI *
October 20, 2009 10:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3154 278
Size: 53 83 186 936 1618 2468
Fragments arranged by size:
2468 1618 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2687 1937
Size: 750 4594
Acc65I G'GTAC_C
Cuts at: 2665 2665
Size: 5344
AclI AA'CG_TT
Cuts at: 1563 3471 3844 1563
Size: 1908 373 3063
Fragments arranged by size:
3063 1908 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2246 3407 4791 5059 1632
Size: 614 1161 1384 268 1917
Fragments arranged by size:
1917 1384 1161 614 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5147
AflIII A'CryG_T
Cuts at: 1203 4243 5220 1203
Size: 3040 977 1327
Fragments arranged by size:
3040 1327 977
AgeI A'CCGG_T
Cuts at: 4175 4175
Size: 5344
AhdI GACnn_n'nnGTC
Cuts at: 4073 4073
Size: 5344
AleI CACnn'nnGTG
Cuts at: 1166 2615 1166
Size: 1449 3895
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2438 1184
Size: 1254 4090
AlwNI CAG_nnn'CTG
Cuts at: 2162 3087 4659 2162
Size: 925 1572 2847
Fragments arranged by size:
2847 1572 925
ApaLI G'TGCA_C
Cuts at: 3401 4557 3401
Size: 1156 4188
ApoI r'AATT_y
Cuts at: 1493 2168 2653 2879 3031 5140 1493
Size: 675 485 226 152 2109 1697
Fragments arranged by size:
2109 1697 675 485 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3898 160
Size: 872 2866 1606
Fragments arranged by size:
2866 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5344
AvaI C'yCGr_G
Cuts at: 1870 1943 2669 1870
Size: 73 726 4545
Fragments arranged by size:
4545 726 73
BamHI G'GATC_C
Cuts at: 2098 2674 3041 2098
Size: 576 367 4401
Fragments arranged by size:
4401 576 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2414 2665 4120 618
Size: 325 217 627 627 251 1455 1842
Fragments arranged by size:
1842 1455 627 627 325 251 217
BanII G_rGCy'C
Cuts at: 1391 2663 1391
Size: 1272 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4373
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3535 3569 1903
Size: 34 1598 34 3678
Fragments arranged by size:
3678 1598 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2402 2546 3236 4446 2402
Size: 144 690 1210 3300
Fragments arranged by size:
3300 1210 690 144
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5344
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3955 136
Size: 107 122 71 631 605 2283 1525
Fragments arranged by size:
2283 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5339 5339
Size: 5344
BlpI GC'TnA_GC
Cuts at: 2761 2761
Size: 5344
Bme1580I G_kGCm'C
Cuts at: 1790 3405 4561 1790
Size: 1615 1156 2573
Fragments arranged by size:
2573 1615 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5344
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3075 4033 442
Size: 416 53 83 399 24 1658 958 1753
Fragments arranged by size:
1753 1658 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5344
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4004 1239
Size: 2765 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2624 3469 4334 4632 2624
Size: 845 865 298 3336
Fragments arranged by size:
3336 865 845 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4007 882
Size: 617 2508 2219
Fragments arranged by size:
2508 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3135 493
Size: 711 1931 2702
Fragments arranged by size:
2702 1931 711
BsaBI GATnn'nnATC
Cuts at: 5338 5338
Size: 5344
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3151 3533 4168 275
Size: 53 83 186 936 1618 382 635 1451
Fragments arranged by size:
1618 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3777 4175 4449 4596 5007 3777
Size: 398 274 147 411 4114
Fragments arranged by size:
4114 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2267 2297 1178
Size: 30 165 30 864 30 4225
Fragments arranged by size:
4225 864 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2285 1186
Size: 1099 4245
BseYI C'CCAG_C
Cuts at: 4547 5043 4547
Size: 496 4848
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4771
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2794 3555 4583 1540
Size: 275 186 793 761 1028 2301
Fragments arranged by size:
2301 1028 793 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3405 3490 4561 3405
Size: 85 1071 4188
Fragments arranged by size:
4188 1071 85
BsmI GAATG_Cn'
Cuts at: 2855 2948 2855
Size: 93 5251
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2663 3405 3490 4561 1391
Size: 399 873 742 85 1071 2174
Fragments arranged by size:
2174 1071 873 742 399 85
BspEI T'CCGG_A
Cuts at: 5007 5007
Size: 5344
BspHI T'CATG_A
Cuts at: 2319 3233 4963 2319
Size: 914 1730 2700
Fragments arranged by size:
2700 1730 914
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2699 844
Size: 1855 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3231 1394
Size: 429 1408 3507
Fragments arranged by size:
3507 1408 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3839 4013 65
Size: 1183 2591 174 1396
Fragments arranged by size:
2591 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2511 3988 4175 1700
Size: 110 701 1477 187 2869
Fragments arranged by size:
2869 1477 701 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5344
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5344
BssSI C'ACGA_G
Cuts at: 3404 4416 3404
Size: 1012 4332
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2240 794
Size: 1446 3898
BstBI TT'CG_AA
Cuts at: 4148 4148
Size: 5344
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5344
BstYI r'GATC_y
Cuts at: 1972 2098 2674 3041 3426 3443 4895 4981 4993
Size: 126 576 367 385 17 1452 86 12
Cuts at: 4993 5339 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 576 385 367 346 126 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2517 2517
Size: 5344
BtgI C'CryG_G
Cuts at: 513 1065 2004 2068 2475 513
Size: 552 939 64 407 3382
Fragments arranged by size:
3382 939 552 407 64
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4605
Fragments arranged by size:
4605 683 56
BtsI GCAGTG_nn'
Cuts at: 2092 2860 3654 3674 2092
Size: 768 794 20 3762
Fragments arranged by size:
3762 794 768 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5344
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5309
DraI TTT'AAA
Cuts at: 2648 2648
Size: 5344
DraIII CAC_nnn'GTG
Cuts at: 2313 2313
Size: 5344
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4351 809
Size: 507 3035 1802
Fragments arranged by size:
3035 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2791 3680 5309
Size: 54 1425 50 275 18 961 889 1629
Cuts at: 5309 8
Size: 43
Fragments arranged by size:
1629 1425 961 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2791 1537
Size: 275 979 4090
Fragments arranged by size:
4090 979 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3274 1353
Size: 1921 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3931 4305 4451 387
Size: 1064 138 2342 374 146 1280
Fragments arranged by size:
2342 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2246 3407 4004 4791 5059 1239
Size: 393 614 1161 597 787 268 1524
Fragments arranged by size:
1524 1161 787 614 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2060 2290 2060
Size: 230 5114
EcoRI G'AATT_C
Cuts at: 2653 3031 2653
Size: 378 4966
EcoRV GAT'ATC
Cuts at: 2331 2331
Size: 5344
FspI TGC'GCA
Cuts at: 3850 3850
Size: 5344
HaeII r_GCGC'y
Cuts at: 1678 4491 1678
Size: 2813 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3985 4017 4059 4091 1373
Size: 32 135 32 2413 32 42 32 2626
Fragments arranged by size:
2626 2413 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2688 2934 669
Size: 988 281 750 246 3079
Fragments arranged by size:
3079 988 750 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5344
HpaI GTT'AAC
Cuts at: 2934 2934
Size: 5344
KpnI G_GTAC'C
Cuts at: 2669 2669
Size: 5344
MfeI C'AATT_G
Cuts at: 2943 2943
Size: 5344
MluI A'CGCG_T
Cuts at: 5220 5220
Size: 5344
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4458 4642 1305
Size: 3153 184 2007
Fragments arranged by size:
3153 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3522
Fragments arranged by size:
3522 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2615 3302 3661 3820 518
Size: 648 1449 687 359 159 2042
Fragments arranged by size:
2042 1449 687 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2542 2760 3437 4585 4830 1606
Size: 936 218 677 1148 245 2120
Fragments arranged by size:
2120 1148 936 677 245 218
NcoI C'CATG_G
Cuts at: 513 1065 2004 2068 2475 513
Size: 552 939 64 407 3382
Fragments arranged by size:
3382 939 552 407 64
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5344
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5344
NotI GC'GGCC_GC
Cuts at: 2791 2791
Size: 5344
NspI r_CATG'y
Cuts at: 1132 1229 2702 4247 1132
Size: 97 1473 1545 2229
Fragments arranged by size:
2229 1545 1473 97
PasI CC'CwG_GG
Cuts at: 1348 2287 1348
Size: 939 4405
PciI A'CATG_T
Cuts at: 4243 4243
Size: 5344
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5344
PfoI T'CCnGG_A
Cuts at: 2592 2592
Size: 5344
PmeI GTTT'AAAC
Cuts at: 2648 2648
Size: 5344
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3413 3445 4926 4958 3413
Size: 32 1481 32 3799
Fragments arranged by size:
3799 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5344
PsiI TTA'TAA
Cuts at: 2914 2914
Size: 5344
PstI C_TGCA'G
Cuts at: 830 2087 2696 830
Size: 1257 609 3478
Fragments arranged by size:
3478 1257 609
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5344
SalI G'TCGA_C
Cuts at: 1936 2686 1936
Size: 750 4594
SbfI CC_TGCA'GG
Cuts at: 2696 2696
Size: 5344
ScaI AGT'ACT
Cuts at: 3592 5304 3592
Size: 1712 3632
SfcI C'TryA_G
Cuts at: 826 1045 2083 2692 3827 4508 4699 826
Size: 219 1038 609 1135 681 191 1471
Fragments arranged by size:
1471 1135 1038 681 609 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5344
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5344
SmaI CCC'GGG
Cuts at: 1872 2671 1872
Size: 799 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2639 3448 4349 4611 820
Size: 197 926 696 809 901 262 1553
Fragments arranged by size:
1553 926 901 809 696 262 197
SnaBI TAC'GTA
Cuts at: 493 3135 493
Size: 2642 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5344
SphI G_CATG'C
Cuts at: 1132 2702 1132
Size: 1570 3774
SspI AAT'ATT
Cuts at: 5 52 3268 5
Size: 47 3216 2081
Fragments arranged by size:
3216 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5344
StyI C'CwwG_G
Cuts at: 513 1065 2004 2068 2475 2505 2783 513
Size: 552 939 64 407 30 278 3074
Fragments arranged by size:
3074 939 552 407 278 64 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2101 2143 3371 3388 3541 3726 4171
Size: 1255 521 42 1228 17 153 185 445
Cuts at: 4171 325
Size: 1498
Fragments arranged by size:
1498 1255 1228 521 445 185 153 42
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2280 3590 5302
Size: 274 80 33 51 1443 303 1310 1712
Cuts at: 5302 96
Size: 138
Fragments arranged by size:
1712 1443 1310 303 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2034 2066 2455 2487 5304 5336 2034
Size: 32 389 32 2817 32 2042
Fragments arranged by size:
2817 2042 389 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5344
XbaI T'CTAG_A
Cuts at: 2680 2680
Size: 5344
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2425 2546 2425
Size: 121 5223
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5344
XmaI C'CCGG_G
Cuts at: 1870 2669 1870
Size: 799 4545
XmnI GAAnn'nnTTC
Cuts at: 3473 3473
Size: 5344
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3152 276
Size: 53 83 186 936 1618 2468
Fragments arranged by size:
2468 1618 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI