Restriction map for
pFN21AE0399
(Circular) (Six-base) MAPSORT of: pFN21AE0399.seq Check: 8558 from: 1 to: 8020
pFN21AE0399 8020 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5272 5272
Size: 8020
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5830 278
Size: 53 83 186 936 4294 2468
Fragments arranged by size:
4294 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3634 5363 1937
Size: 1697 1729 4594
Fragments arranged by size:
4594 1729 1697
Acc65I G'GTAC_C
Cuts at: 4189 5197 5341 4189
Size: 1008 144 6868
Fragments arranged by size:
6868 1008 144
AclI AA'CG_TT
Cuts at: 1563 6147 6520 1563
Size: 4584 373 3063
Fragments arranged by size:
4584 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2243 2558 4051 4353 4530 4865 6083 7467
Size: 611 315 1493 302 177 335 1218 1384
Cuts at: 7467 7735 1632
Size: 268 1917
Fragments arranged by size:
1917 1493 1384 1218 611 335 315 302
268 177
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7823
AflIII A'CryG_T
Cuts at: 1203 3492 3946 4535 6919 7896 1203
Size: 2289 454 589 2384 977 1327
Fragments arranged by size:
2384 2289 1327 977 589 454
AgeI A'CCGG_T
Cuts at: 2665 6851 2665
Size: 4186 3834
AhdI GACnn_n'nnGTC
Cuts at: 6749 6749
Size: 8020
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8020
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4674 1184
Size: 3490 4530
AlwNI CAG_nnn'CTG
Cuts at: 4619 5763 7335 4619
Size: 1144 1572 5304
Fragments arranged by size:
5304 1572 1144
ApaI G_GGCC'C
Cuts at: 2117 4305 2117
Size: 2188 5832
ApaLI G'TGCA_C
Cuts at: 6077 7233 6077
Size: 1156 6864
ApoI r'AATT_y
Cuts at: 1493 3478 3754 4021 4584 5329 5555 5707 7816
Size: 1985 276 267 563 745 226 152 2109
Cuts at: 7816 1493
Size: 1697
Fragments arranged by size:
2109 1985 1697 745 563 276 267 226
152
AseI AT'TA_AT
Cuts at: 160 1032 6574 160
Size: 872 5542 1606
Fragments arranged by size:
5542 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8020
AvaI C'yCGr_G
Cuts at: 1870 1943 2059 4173 5078 5193 5345 1870
Size: 73 116 2114 905 115 152 4545
Fragments arranged by size:
4545 2114 905 152 116 115 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2718 2751 3117 3150 2718
Size: 33 366 33 7588
Fragments arranged by size:
7588 366 33 33
BamHI G'GATC_C
Cuts at: 4258 5350 5717 4258
Size: 1092 367 6561
Fragments arranged by size:
6561 1092 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2048 2351 4189 4962 5197
Size: 325 217 627 261 303 1838 773 235
Cuts at: 5197 5341 6796 618
Size: 144 1455 1842
Fragments arranged by size:
1842 1838 1455 773 627 325 303 261
235 217 144
BanII G_rGCy'C
Cuts at: 1391 2060 2117 2141 4305 5339 1391
Size: 669 57 24 2164 1034 4072
Fragments arranged by size:
4072 2164 1034 669 57 24
BbeI G_GCGC'C
Cuts at: 2052 2355 2052
Size: 303 7717
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3126 928
Size: 971 1227 5822
Fragments arranged by size:
5822 1227 971
BbvCI CC'TCA_GC
Cuts at: 4630 5308 4630
Size: 678 7342
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6211 6245 1903
Size: 34 4274 34 3678
Fragments arranged by size:
4274 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4477 5235 5912 7122 4477
Size: 758 677 1210 5375
Fragments arranged by size:
5375 1210 758 677
BclI T'GATC_A
Cuts at: 1548 3292 1548
Size: 1744 6276
BfrBI ATG'CAT
Cuts at: 3368 3975 3368
Size: 607 7413
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2015 4634 6631
Size: 107 122 71 631 605 343 2619 1997
Cuts at: 6631 136
Size: 1525
Fragments arranged by size:
2619 1997 1525 631 605 343 122 107
71
BglII A'GATC_T
Cuts at: 3100 8015 3100
Size: 4915 3105
BlpI GC'TnA_GC
Cuts at: 5437 5437
Size: 8020
Bme1580I G_kGCm'C
Cuts at: 1790 2117 4305 4965 6081 7237 1790
Size: 327 2188 660 1116 1156 2573
Fragments arranged by size:
2573 2188 1156 1116 660 327
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8020
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4569 5751 6709
Size: 416 53 83 399 24 3152 1182 958
Cuts at: 6709 442
Size: 1753
Fragments arranged by size:
3152 1753 1182 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3054 1056
Size: 1998 6022
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2367 3438 3573 3920 3929 4295 6680 1239
Size: 1128 1071 135 347 9 366 2385 2579
Fragments arranged by size:
2579 2385 1128 1071 366 347 135 9
Bpu10I CC'TnA_GC
Cuts at: 4630 5308 4630
Size: 678 7342
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4037 6145 7010 7308 4037
Size: 2108 865 298 4749
Fragments arranged by size:
4749 2108 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2656 3809 3878 6683 882
Size: 617 1157 1153 69 2805 2219
Fragments arranged by size:
2805 2219 1157 1153 617 69
BsaAI yAC'GTr
Cuts at: 493 1204 3495 5811 493
Size: 711 2291 2316 2702
Fragments arranged by size:
2702 2316 2291 711
BsaBI GATnn'nnATC
Cuts at: 3276 8014 3276
Size: 4738 3282
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2049 2352 5827 6209
Size: 53 83 186 936 516 303 3475 382
Cuts at: 6209 6844 275
Size: 635 1451
Fragments arranged by size:
3475 1451 936 635 516 382 303 186
83 53
BsaWI w'CCGG_w
Cuts at: 2665 6453 6851 7125 7272 7683 2665
Size: 3788 398 274 147 411 3002
Fragments arranged by size:
3788 3002 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2135 2165 3201 3231 3806
Size: 30 165 30 732 30 1036 30 575
Cuts at: 3806 3836 1178
Size: 30 5362
Fragments arranged by size:
5362 1036 732 575 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2864 3645 3654 4178 4199 1186
Size: 1678 781 9 524 21 5007
Fragments arranged by size:
5007 1678 781 524 21 9
BseYI C'CCAG_C
Cuts at: 2154 2179 2237 5040 7223 7719 2154
Size: 25 58 2803 2183 496 2455
Fragments arranged by size:
2803 2455 2183 496 58 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2270 2504 1163
Size: 573 534 234 6679
Fragments arranged by size:
6679 573 534 234
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5470 6231 7259 1540
Size: 275 186 3469 761 1028 2301
Fragments arranged by size:
3469 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3415 4867 6081 6166 7237 3415
Size: 1452 1214 85 1071 4198
Fragments arranged by size:
4198 1452 1214 1071 85
BsmI GAATG_Cn'
Cuts at: 3977 5531 5624 3977
Size: 1554 93 6373
Fragments arranged by size:
6373 1554 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2138 2138
Size: 8020
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2060 2117 2141 3415 4305 4867 4965
Size: 399 270 57 24 1274 890 562 98
Cuts at: 4965 5339 6081 6166 7237 1391
Size: 374 742 85 1071 2174
Fragments arranged by size:
2174 1274 1071 890 742 562 399 374
270 98 85 57 24
BspEI T'CCGG_A
Cuts at: 7683 7683
Size: 8020
BspHI T'CATG_A
Cuts at: 5909 7639 5909
Size: 1730 6290
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5272 5375 844
Size: 4428 103 3489
Fragments arranged by size:
4428 3489 103
BsrBI CCG'CTC
Cuts at: 1394 1823 5907 1394
Size: 429 4084 3507
Fragments arranged by size:
4084 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3651 4287 6515 6689 65
Size: 1183 2403 636 2228 174 1396
Fragments arranged by size:
2403 2228 1396 1183 636 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2665 6664 6851 1700
Size: 110 855 3999 187 2869
Fragments arranged by size:
3999 2869 855 187 110
BsrGI T'GTAC_A
Cuts at: 96 3915 96
Size: 3819 4201
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8020
BssSI C'ACGA_G
Cuts at: 4239 6080 7092 4239
Size: 1841 1012 5167
Fragments arranged by size:
5167 1841 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4142 4619 4807 5089 794
Size: 3348 477 188 282 3725
Fragments arranged by size:
3725 3348 477 282 188
BstBI TT'CG_AA
Cuts at: 6824 6824
Size: 8020
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8020
BstXI CCAn_nnnn'nTGG
Cuts at: 3115 3646 4654 5135 3115
Size: 531 1008 481 6000
Fragments arranged by size:
6000 1008 531 481
BstYI r'GATC_y
Cuts at: 1972 2767 3100 3223 3277 4258 5350 5717 6102
Size: 795 333 123 54 981 1092 367 385
Cuts at: 6102 6119 7571 7657 7669 8015 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1092 981 795 385 367 346
333 123 86 54 17 12
Bsu36I CC'TnA_GG
Cuts at: 3726 3726
Size: 8020
BtgI C'CryG_G
Cuts at: 513 1065 2004 2052 2789 3639 513
Size: 552 939 48 737 850 4894
Fragments arranged by size:
4894 939 850 737 552 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7281
Fragments arranged by size:
7281 683 56
BtsI GCAGTG_nn'
Cuts at: 3528 3602 4668 5536 6330 6350 3528
Size: 74 1066 868 794 20 5198
Fragments arranged by size:
5198 1066 868 794 74 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8020
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4182 4217 529
Size: 35 3618 35 4332
Fragments arranged by size:
4332 3618 35 35
DraI TTT'AAA
Cuts at: 5324 5324
Size: 8020
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7027 809
Size: 507 5711 1802
Fragments arranged by size:
5711 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2118 4894
Size: 54 1425 50 275 18 177 111 2776
Cuts at: 4894 5467 6356 7985 8
Size: 573 889 1629 43
Fragments arranged by size:
2776 1629 1425 889 573 275 177 111
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5467 1537
Size: 275 3655 4090
Fragments arranged by size:
4090 3655 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2913 2938 3023 3420 3859 4329 4506 4743
Size: 1560 25 85 397 439 470 177 237
Cuts at: 4743 5950 1353
Size: 1207 3423
Fragments arranged by size:
3423 1560 1207 470 439 397 237 177
85 25
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2422 5271 6607 6981 7127 387
Size: 1064 138 833 2849 1336 374 146 1280
Fragments arranged by size:
2849 1336 1280 1064 833 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2243 2367 2558 3438 3573 3920 3929
Size: 393 611 124 191 880 135 347 9
Cuts at: 3929 4051 4295 4353 4530 4865 6083 6680 7467
Size: 122 244 58 177 335 1218 597 787
Cuts at: 7467 7735 1239
Size: 268 1524
Fragments arranged by size:
1524 1218 880 787 611 597 393 347
335 268 244 191 177 135 124 122
58 9
EcoNI CCTnn'n_nnAGG
Cuts at: 3204 3285 3204
Size: 81 7939
EcoO109I rG'GnC_Cy
Cuts at: 2113 3650 3904 4006 4301 4931 5189 5275 2113
Size: 1537 254 102 295 630 258 86 4858
Fragments arranged by size:
4858 1537 630 295 258 254 102 86
EcoRI G'AATT_C
Cuts at: 4021 5329 5707 4021
Size: 1308 378 6334
Fragments arranged by size:
6334 1308 378
EcoRV GAT'ATC
Cuts at: 2563 2563
Size: 8020
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3071 3173 3071
Size: 102 7918
FspI TGC'GCA
Cuts at: 6526 6526
Size: 8020
HaeII r_GCGC'y
Cuts at: 1678 2052 2355 7167 1678
Size: 374 303 4812 2531
Fragments arranged by size:
4812 2531 374 303
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2135 2167 2496 2528 2585
Size: 32 135 32 563 32 329 32 57
Cuts at: 2585 2617 2878 2910 3677 3709 3806 3838 4475
Size: 32 261 32 767 32 97 32 637
Cuts at: 4475 4507 4966 4998 6661 6693 6735 6767 1373
Size: 32 459 32 1663 32 42 32 2626
Fragments arranged by size:
2626 1663 767 637 563 459 329 261
135 97 57 42 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3310 3622 5364 5610 669
Size: 988 281 1372 312 1742 246 3079
Fragments arranged by size:
3079 1742 1372 988 312 281 246
HindIII A'AGCT_T
Cuts at: 748 2607 4319 748
Size: 1859 1712 4449
Fragments arranged by size:
4449 1859 1712
HpaI GTT'AAC
Cuts at: 5610 5610
Size: 8020
KasI G'GCGC_C
Cuts at: 2048 2351 2048
Size: 303 7717
KpnI G_GTAC'C
Cuts at: 4193 5201 5345 4193
Size: 1008 144 6868
Fragments arranged by size:
6868 1008 144
MfeI C'AATT_G
Cuts at: 2491 5619 2491
Size: 3128 4892
MluI A'CGCG_T
Cuts at: 7896 7896
Size: 8020
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3029 3518 3921 3931 7134 7318 1305
Size: 1724 489 403 10 3203 184 2007
Fragments arranged by size:
3203 2007 1724 489 403 184 10
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4896 10
Size: 54 1425 343 3064 3134
Fragments arranged by size:
3134 3064 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2211 2742 3644 3672 5978 6337 6496
Size: 648 1045 531 902 28 2306 359 159
Cuts at: 6496 518
Size: 2042
Fragments arranged by size:
2306 2042 1045 902 648 531 359 159
28
MspA1I CmG'CkG
Cuts at: 1606 2015 2042 2158 2899 4284 4619 4634 4843
Size: 409 27 116 741 1385 335 15 209
Cuts at: 4843 5312 5436 6113 7261 7506 1606
Size: 469 124 677 1148 245 2120
Fragments arranged by size:
2120 1385 1148 741 677 469 409 335
245 209 124 116 27 15
NarI GG'CG_CC
Cuts at: 2049 2352 2049
Size: 303 7717
NcoI C'CATG_G
Cuts at: 513 1065 2004 2789 3639 513
Size: 552 939 785 850 4894
Fragments arranged by size:
4894 939 850 785 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8020
NheI G'CTAG_C
Cuts at: 1052 3050 1052
Size: 1998 6022
NotI GC'GGCC_GC
Cuts at: 5467 5467
Size: 8020
NsiI A_TGCA'T
Cuts at: 3370 3977 3370
Size: 607 7413
NspI r_CATG'y
Cuts at: 1132 1229 2291 3950 4539 5092 5164 5224 5378
Size: 97 1062 1659 589 553 72 60 154
Cuts at: 5378 6923 1132
Size: 1545 2229
Fragments arranged by size:
2229 1659 1545 1062 589 553 154 97
72 60
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8020
PciI A'CATG_T
Cuts at: 3946 4535 6919 3946
Size: 589 2384 5047
Fragments arranged by size:
5047 2384 589
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2130 1108
Size: 1022 6998
PfoI T'CCnGG_A
Cuts at: 2265 3897 4179 2265
Size: 1632 282 6106
Fragments arranged by size:
6106 1632 282
PmeI GTTT'AAAC
Cuts at: 5324 5324
Size: 8020
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6089 6121 7602 7634 6089
Size: 32 1481 32 6475
Fragments arranged by size:
6475 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3650 4006 4931 5189 5275 3650
Size: 356 925 258 86 6395
Fragments arranged by size:
6395 925 356 258 86
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8020
PsiI TTA'TAA
Cuts at: 3384 5590 3384
Size: 2206 5814
PspOMI G'GGCC_C
Cuts at: 2113 4301 2113
Size: 2188 5832
PstI C_TGCA'G
Cuts at: 830 4147 4663 5269 5372 830
Size: 3317 516 606 103 3478
Fragments arranged by size:
3478 3317 606 516 103
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8020
PvuII CAG'CTG
Cuts at: 2158 4284 4619 4843 5312 2158
Size: 2126 335 224 469 4866
Fragments arranged by size:
4866 2126 469 335 224
SalI G'TCGA_C
Cuts at: 1936 5362 1936
Size: 3426 4594
SanDI GG'GwC_CC
Cuts at: 5275 5275
Size: 8020
SapI GCTCTTCn'nnn_
Cuts at: 2913 3420 4743 2913
Size: 507 1323 6190
Fragments arranged by size:
6190 1323 507
SbfI CC_TGCA'GG
Cuts at: 5372 5372
Size: 8020
ScaI AGT'ACT
Cuts at: 3156 4096 6268 7980 3156
Size: 940 2172 1712 3196
Fragments arranged by size:
3196 2172 1712 940
SfcI C'TryA_G
Cuts at: 826 1045 2420 3216 4143 4659 5265 5368 6503
Size: 219 1375 796 927 516 606 103 1135
Cuts at: 6503 7184 7375 826
Size: 681 191 1471
Fragments arranged by size:
1471 1375 1135 927 796 681 606 516
219 191 103
SfoI GGC'GCC
Cuts at: 2050 2353 2050
Size: 303 7717
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8020
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8020
SmaI CCC'GGG
Cuts at: 1872 5080 5195 5347 1872
Size: 3208 115 152 4545
Fragments arranged by size:
4545 3208 152 115
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4016 6124 7025 7287 820
Size: 197 926 2073 2108 901 262 1553
Fragments arranged by size:
2108 2073 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5811 493
Size: 5318 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8020
SphI G_CATG'C
Cuts at: 1132 5164 5378 1132
Size: 4032 214 3774
Fragments arranged by size:
4032 3774 214
SrfI GCCC'GGGC
Cuts at: 5080 5080
Size: 8020
SspI AAT'ATT
Cuts at: 5 52 5944 5
Size: 47 5892 2081
Fragments arranged by size:
5892 2081 47
StuI AGG'CCT
Cuts at: 1517 2085 1517
Size: 568 7452
StyI C'CwwG_G
Cuts at: 513 1065 2004 2789 3108 3313 3639 4036 5459
Size: 552 939 785 319 205 326 397 1423
Cuts at: 5459 513
Size: 3074
Fragments arranged by size:
3074 1423 939 785 552 397 326 319
205
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 6047 6064 6217 6402 6847 325
Size: 1255 4467 17 153 185 445 1498
Fragments arranged by size:
4467 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3154 3915 4094
Size: 274 80 33 51 1443 1177 761 179
Cuts at: 4094 4225 6266 7978 96
Size: 131 2041 1712 138
Fragments arranged by size:
2041 1712 1443 1177 761 274 179 138
131 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3016 3048 7980 8012 3016
Size: 32 4932 32 3024
Fragments arranged by size:
4932 3024 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2149 1316
Size: 833 7187
XbaI T'CTAG_A
Cuts at: 3226 4786 5356 3226
Size: 1560 570 5890
Fragments arranged by size:
5890 1560 570
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2230 2787 4270 2230
Size: 557 1483 5980
Fragments arranged by size:
5980 1483 557
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8020
XmaI C'CCGG_G
Cuts at: 1870 5078 5193 5345 1870
Size: 3208 115 152 4545
Fragments arranged by size:
4545 3208 152 115
XmnI GAAnn'nnTTC
Cuts at: 6149 6149
Size: 8020
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5828 276
Size: 53 83 186 936 4294 2468
Fragments arranged by size:
4294 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BplI BsiWI BstZ17I DraIII
EcoICRI FseI FspAI NaeI NgoMIV NruI PacI PmlI
PspXI PsrI RsrII SacI SacII SexAI SfiI SwaI