Restriction map for
pFN21AE0396
(Circular) (Six-base) MAPSORT of: pFN21AE0396.seq Check: 5002 from: 1 to: 7825
pFN21AE0396 7825 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3465 3465
Size: 7825
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5635 278
Size: 53 83 186 936 4099 2468
Fragments arranged by size:
4099 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5168 1937
Size: 3231 4594
Acc65I G'GTAC_C
Cuts at: 5146 5146
Size: 7825
AclI AA'CG_TT
Cuts at: 1563 5952 6325 1563
Size: 4389 373 3063
Fragments arranged by size:
4389 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2833 3272 4001 4785 4961 4988 5888 7272
Size: 1201 439 729 784 176 27 900 1384
Cuts at: 7272 7540 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1201 900 784 729 439 268
176 27
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7628
AflIII A'CryG_T
Cuts at: 1203 2608 3693 6724 7701 1203
Size: 1405 1085 3031 977 1327
Fragments arranged by size:
3031 1405 1327 1085 977
AgeI A'CCGG_T
Cuts at: 6656 6656
Size: 7825
AhdI GACnn_n'nnGTC
Cuts at: 2347 3279 6554 2347
Size: 932 3275 3618
Fragments arranged by size:
3618 3275 932
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7825
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2945 1184
Size: 1761 6064
AlwNI CAG_nnn'CTG
Cuts at: 2938 3220 3499 4435 4714 4759 5568 7140 2938
Size: 282 279 936 279 45 809 1572 3623
Fragments arranged by size:
3623 1572 936 809 282 279 279 45
ApaI G_GGCC'C
Cuts at: 4331 4753 4331
Size: 422 7403
ApaLI G'TGCA_C
Cuts at: 4581 5882 7038 4581
Size: 1301 1156 5368
Fragments arranged by size:
5368 1301 1156
ApoI r'AATT_y
Cuts at: 1493 2975 3803 4948 5134 5360 5512 7621 1493
Size: 1482 828 1145 186 226 152 2109 1697
Fragments arranged by size:
2109 1697 1482 1145 828 226 186 152
AseI AT'TA_AT
Cuts at: 160 1032 6379 160
Size: 872 5347 1606
Fragments arranged by size:
5347 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7825
AvaI C'yCGr_G
Cuts at: 1870 1943 2397 3392 4053 4214 5150 1870
Size: 73 454 995 661 161 936 4545
Fragments arranged by size:
4545 995 936 661 454 161 73
AvrII C'CTAG_G
Cuts at: 4996 4996
Size: 7825
BamHI G'GATC_C
Cuts at: 5155 5522 5155
Size: 367 7458
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2262 3793 4110 4299 4832
Size: 325 217 627 475 1531 317 189 533
Cuts at: 4832 5146 6601 618
Size: 314 1455 1842
Fragments arranged by size:
1842 1531 1455 627 533 475 325 317
314 217 189
BanII G_rGCy'C
Cuts at: 1391 2822 2847 3393 4331 4669 4689 4753 5144
Size: 1431 25 546 938 338 20 64 391
Cuts at: 5144 1391
Size: 4072
Fragments arranged by size:
4072 1431 938 546 391 338 64 25
20
BbeI G_GCGC'C
Cuts at: 2266 3797 4114 2266
Size: 1531 317 5977
Fragments arranged by size:
5977 1531 317
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2980 3093 3457 3611 928
Size: 971 1081 113 364 154 5142
Fragments arranged by size:
5142 1081 971 364 154 113
BbvCI CC'TCA_GC
Cuts at: 2681 3433 2681
Size: 752 7073
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4482 4516 6016 6050 1903
Size: 34 2545 34 1500 34 3678
Fragments arranged by size:
3678 2545 1500 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5717 6927 5717
Size: 1210 6615
BclI T'GATC_A
Cuts at: 1548 4777 1548
Size: 3229 4596
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4037 6436 136
Size: 107 122 71 631 605 2365 2399 1525
Fragments arranged by size:
2399 2365 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7820 7820
Size: 7825
BlpI GC'TnA_GC
Cuts at: 4596 4799 5242 4596
Size: 203 443 7179
Fragments arranged by size:
7179 443 203
Bme1580I G_kGCm'C
Cuts at: 1790 4126 4302 4331 4585 4753 4837 5886 7042
Size: 2336 176 29 254 168 84 1049 1156
Cuts at: 7042 1790
Size: 2573
Fragments arranged by size:
2573 2336 1156 1049 254 176 168 84
29
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7825
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3609 4072 5556
Size: 416 53 83 399 24 2192 463 1484
Cuts at: 5556 6514 442
Size: 958 1753
Fragments arranged by size:
2192 1753 1484 958 463 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7825
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2218 2218
Size: 7825
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2768 2795 2858 3698 4889 4910 6485 1239
Size: 1529 27 63 840 1191 21 1575 2579
Fragments arranged by size:
2579 1575 1529 1191 840 63 27 21
Bpu10I CC'TnA_GC
Cuts at: 2681 3433 2681
Size: 752 7073
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3320 4289 4810 5950 6815 7113 3320
Size: 969 521 1140 865 298 4032
Fragments arranged by size:
4032 1140 969 865 521 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2146 2394 3422 6488 882
Size: 617 647 248 1028 3066 2219
Fragments arranged by size:
3066 2219 1028 647 617 248
BsaAI yAC'GTr
Cuts at: 493 1204 3694 4283 5616 493
Size: 711 2490 589 1333 2702
Fragments arranged by size:
2702 2490 1333 711 589
BsaBI GATnn'nnATC
Cuts at: 3947 7819 3947
Size: 3872 3953
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2263 3794 4111 5632
Size: 53 83 186 936 730 1531 317 1521
Cuts at: 5632 6014 6649 275
Size: 382 635 1451
Fragments arranged by size:
1531 1521 1451 936 730 635 382 317
186 83 53
BsaWI w'CCGG_w
Cuts at: 4233 6258 6656 6930 7077 7488 4233
Size: 2025 398 274 147 411 4570
Fragments arranged by size:
4570 2025 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3503 3533 4148 4178 4656
Size: 30 165 30 2100 30 615 30 478
Cuts at: 4656 4686 1178
Size: 30 4317
Fragments arranged by size:
4317 2100 615 478 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2051 2054 2131 2855 4271 4907 5096 1186
Size: 865 3 77 724 1416 636 189 3915
Fragments arranged by size:
3915 1416 865 724 636 189 77 3
BseYI C'CCAG_C
Cuts at: 2370 2392 2821 3705 4636 7028 7524 2370
Size: 22 429 884 931 2392 496 2671
Fragments arranged by size:
2671 2392 931 884 496 429 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2924 3425 3632 4164 4263 1163
Size: 573 1188 501 207 532 99 4725
Fragments arranged by size:
4725 1188 573 532 501 207 99
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3198 3288 5275 6036 7064 1540
Size: 275 186 1197 90 1987 761 1028 2301
Fragments arranged by size:
2301 1987 1197 1028 761 275 186 90
BsiHKAI G_wGCw'C
Cuts at: 2093 2847 2924 3060 4062 4522 4585 5886 5971
Size: 754 77 136 1002 460 63 1301 85
Cuts at: 5971 7042 2093
Size: 1071 2876
Fragments arranged by size:
2876 1301 1071 1002 754 460 136 85
77 63
BsmI GAATG_Cn'
Cuts at: 3262 5336 5429 3262
Size: 2074 93 5658
Fragments arranged by size:
5658 2074 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2729 2729
Size: 7825
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2093 2822 2847 2924 3060 3393 4062
Size: 399 303 729 25 77 136 333 669
Cuts at: 4062 4126 4302 4331 4522 4585 4669 4689 4753
Size: 64 176 29 191 63 84 20 64
Cuts at: 4753 4837 5144 5886 5971 7042 1391
Size: 84 307 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 729 669 399 333 307
303 191 176 136 85 84 84 77
64 64 63 29 25 20
BspEI T'CCGG_A
Cuts at: 4233 7488 4233
Size: 3255 4570
BspHI T'CATG_A
Cuts at: 3385 5714 7444 3385
Size: 2329 1730 3766
Fragments arranged by size:
3766 2329 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2412 3255 3465 3555 5180 844
Size: 1568 843 210 90 1625 3489
Fragments arranged by size:
3489 1625 1568 843 210 90
BsrBI CCG'CTC
Cuts at: 1394 1823 2479 4261 5712 1394
Size: 429 656 1782 1451 3507
Fragments arranged by size:
3507 1782 1451 656 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6320 6494 65
Size: 1183 5072 174 1396
Fragments arranged by size:
5072 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2415 3003 6469 6656 1700
Size: 110 605 588 3466 187 2869
Fragments arranged by size:
3466 2869 605 588 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7825
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7825
BssSI C'ACGA_G
Cuts at: 5885 6897 5885
Size: 1012 6813
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2938 3568 3687 3857 4210 4435 794
Size: 2144 630 119 170 353 225 4184
Fragments arranged by size:
4184 2144 630 353 225 170 119
BstBI TT'CG_AA
Cuts at: 6629 6629
Size: 7825
BstEII G'GTnAC_C
Cuts at: 1184 3271 1184
Size: 2087 5738
BstYI r'GATC_y
Cuts at: 1972 5155 5522 5907 5924 7376 7462 7474 7820
Size: 3183 367 385 17 1452 86 12 346
Cuts at: 7820 1972
Size: 1977
Fragments arranged by size:
3183 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3305 3371 3305
Size: 66 7759
BtgI C'CryG_G
Cuts at: 513 1065 2004 2568 2688 4380 4590 4659 513
Size: 552 939 564 120 1692 210 69 3679
Fragments arranged by size:
3679 1692 939 564 552 210 120 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2299 487
Size: 683 56 1073 6013
Fragments arranged by size:
6013 1073 683 56
BtsI GCAGTG_nn'
Cuts at: 2917 3020 3058 3184 4327 4525 4653 4831 5341
Size: 103 38 126 1143 198 128 178 510
Cuts at: 5341 6135 6155 2917
Size: 794 20 4587
Fragments arranged by size:
4587 1143 794 510 198 178 128 126
103 38 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7825
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3858 3893 529
Size: 35 3294 35 4461
Fragments arranged by size:
4461 3294 35 35
DraI TTT'AAA
Cuts at: 5129 5129
Size: 7825
DraIII CAC_nnn'GTG
Cuts at: 3857 3945 4187 4338 3857
Size: 88 242 151 7344
Fragments arranged by size:
7344 242 151 88
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2692 6832 809
Size: 507 1376 4140 1802
Fragments arranged by size:
4140 1802 1376 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2256 2417 2673
Size: 54 1425 50 275 18 426 161 256
Cuts at: 2673 2860 3021 3285 3924 4677 5272 6161 7790
Size: 187 161 264 639 753 595 889 1629
Cuts at: 7790 8
Size: 43
Fragments arranged by size:
1629 1425 889 753 639 595 426 275
264 256 187 161 161 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3285 5272 1537
Size: 275 1473 1987 4090
Fragments arranged by size:
4090 1987 1473 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2007 2810 2852 3230 3896 4527 5755 1353
Size: 654 803 42 378 666 631 1228 3423
Fragments arranged by size:
3423 1228 803 666 654 631 378 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3404 3416 6412 6786 6932 387
Size: 1064 138 1815 12 2996 374 146 1280
Fragments arranged by size:
2996 1815 1280 1064 374 146 138 12
EcoICRI GAG'CTC
Cuts at: 2845 2845
Size: 7825
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2768 2795 2833 2858 3272 3698 4001
Size: 393 1136 27 38 25 414 426 303
Cuts at: 4001 4785 4889 4910 4961 4988 5888 6485 7272
Size: 784 104 21 51 27 900 597 787
Cuts at: 7272 7540 1239
Size: 268 1524
Fragments arranged by size:
1524 1136 900 787 784 597 426 414
393 303 268 104 51 38 27 27
25 21
EcoNI CCTnn'n_nnAGG
Cuts at: 3236 5015 3236
Size: 1779 6046
EcoO109I rG'GnC_Cy
Cuts at: 2148 2197 2374 3519 3611 3673 4066 4099 4328
Size: 49 177 1145 92 62 393 33 229
Cuts at: 4328 4749 4756 5020 2148
Size: 421 7 264 4953
Fragments arranged by size:
4953 1145 421 393 264 229 177 92
62 49 33 7
EcoRI G'AATT_C
Cuts at: 3803 5134 5512 3803
Size: 1331 378 6116
Fragments arranged by size:
6116 1331 378
FspI TGC'GCA
Cuts at: 6331 6331
Size: 7825
HaeII r_GCGC'y
Cuts at: 1678 2266 3382 3797 4114 6972 1678
Size: 588 1116 415 317 2858 2531
Fragments arranged by size:
2858 2531 1116 588 415 317
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2040 2072 2333 2365 2366
Size: 32 135 32 468 32 261 32 1
Cuts at: 2366 2398 3191 3223 3265 3297 3503 3535 4388
Size: 32 793 32 42 32 206 32 853
Cuts at: 4388 4420 6466 6498 6540 6572 1373
Size: 32 2046 32 42 32 2626
Fragments arranged by size:
2626 2046 853 793 468 261 206 135
42 42 32 32 32 32 32 32
32 32 32 32 32 1
HincII GTy'rAC
Cuts at: 669 1657 1938 3132 4311 5089 5169 5415 669
Size: 988 281 1194 1179 778 80 246 3079
Fragments arranged by size:
3079 1194 1179 988 778 281 246 80
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7825
HpaI GTT'AAC
Cuts at: 3132 5415 3132
Size: 2283 5542
KasI G'GCGC_C
Cuts at: 2262 3793 4110 2262
Size: 1531 317 5977
Fragments arranged by size:
5977 1531 317
KpnI G_GTAC'C
Cuts at: 5150 5150
Size: 7825
MfeI C'AATT_G
Cuts at: 3895 4535 5424 3895
Size: 640 889 6296
Fragments arranged by size:
6296 889 640
MluI A'CGCG_T
Cuts at: 7701 7701
Size: 7825
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6939 7123 1305
Size: 5634 184 2007
Fragments arranged by size:
5634 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2258 2675 2862 3023 3926
Size: 54 1425 343 426 417 187 161 903
Cuts at: 3926 10
Size: 3909
Fragments arranged by size:
3909 1425 903 426 417 343 187 161
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2445 2868 4421 5783 6142 6301 518
Size: 648 1279 423 1553 1362 359 159 2042
Fragments arranged by size:
2042 1553 1362 1279 648 423 359 159
MspA1I CmG'CkG
Cuts at: 1606 2125 2935 3000 3010 3290 4128 4178 4393
Size: 519 810 65 10 280 838 50 215
Cuts at: 4393 4432 4661 5241 5918 7066 7311 1606
Size: 39 229 580 677 1148 245 2120
Fragments arranged by size:
2120 1148 838 810 677 580 519 280
245 229 215 65 50 39 10
NaeI GCC'GGC
Cuts at: 3005 3005
Size: 7825
NarI GG'CG_CC
Cuts at: 2263 3794 4111 2263
Size: 1531 317 5977
Fragments arranged by size:
5977 1531 317
NcoI C'CATG_G
Cuts at: 513 1065 2004 4590 513
Size: 552 939 2586 3748
Fragments arranged by size:
3748 2586 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7825
NgoMIV G'CCGG_C
Cuts at: 3003 3003
Size: 7825
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7825
NotI GC'GGCC_GC
Cuts at: 5272 5272
Size: 7825
NspI r_CATG'y
Cuts at: 1132 1229 2461 2612 2661 3578 3685 4208 4611
Size: 97 1232 151 49 917 107 523 403
Cuts at: 4611 5183 6728 1132
Size: 572 1545 2229
Fragments arranged by size:
2229 1545 1232 917 572 523 403 151
107 97 49
PasI CC'CwG_GG
Cuts at: 1348 2830 1348
Size: 1482 6343
PciI A'CATG_T
Cuts at: 2608 6724 2608
Size: 4116 3709
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2233 3607 4675 4759 4843 1108
Size: 1125 1374 1068 84 84 4090
Fragments arranged by size:
4090 1374 1125 1068 84 84
PfoI T'CCnGG_A
Cuts at: 2172 2772 2970 4905 5043 2172
Size: 600 198 1935 138 4954
Fragments arranged by size:
4954 1935 600 198 138
PmeI GTTT'AAAC
Cuts at: 5129 5129
Size: 7825
PmlI CAC'GTG
Cuts at: 4283 4283
Size: 7825
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3143 3175 5057 5089 5894 5926 7407 7439 3143
Size: 32 1882 32 805 32 1481 32 3529
Fragments arranged by size:
3529 1882 1481 805 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2148 2197 2374 3519 3611 2148
Size: 49 177 1145 92 6362
Fragments arranged by size:
6362 1145 177 92 49
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7825
PsiI TTA'TAA
Cuts at: 5395 5395
Size: 7825
PspOMI G'GGCC_C
Cuts at: 4327 4749 4327
Size: 422 7403
PstI C_TGCA'G
Cuts at: 830 2409 2934 2959 3015 3240 3510 3561 3996
Size: 1579 525 25 56 225 270 51 435
Cuts at: 3996 4365 4392 5019 5105 5177 830
Size: 369 27 627 86 72 3478
Fragments arranged by size:
3478 1579 627 525 435 369 270 225
86 72 56 51 27 25
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7825
PvuII CAG'CTG
Cuts at: 2125 3000 3010 4178 4393 4432 2125
Size: 875 10 1168 215 39 5518
Fragments arranged by size:
5518 1168 875 215 39 10
RsrII CG'GwC_CG
Cuts at: 3403 3403
Size: 7825
SacI G_AGCT'C
Cuts at: 2847 2847
Size: 7825
SacII CC_GC'GG
Cuts at: 4662 4662
Size: 7825
SalI G'TCGA_C
Cuts at: 1936 5167 1936
Size: 3231 4594
SanDI GG'GwC_CC
Cuts at: 2148 2374 3519 3611 2148
Size: 226 1145 92 6362
Fragments arranged by size:
6362 1145 226 92
SapI GCTCTTCn'nnn_
Cuts at: 2810 2852 3230 4527 2810
Size: 42 378 1297 6108
Fragments arranged by size:
6108 1297 378 42
SbfI CC_TGCA'GG
Cuts at: 5019 5177 5019
Size: 158 7667
ScaI AGT'ACT
Cuts at: 6073 7785 6073
Size: 1712 6113
SfcI C'TryA_G
Cuts at: 826 1045 2405 2930 2955 3011 3236 3506 3534
Size: 219 1360 525 25 56 225 270 28
Cuts at: 3534 3557 3992 4361 4388 5015 5101 5173 6308
Size: 23 435 369 27 627 86 72 1135
Cuts at: 6308 6989 7180 826
Size: 681 191 1471
Fragments arranged by size:
1471 1360 1135 681 627 525 435 369
270 225 219 191 86 72 56 28
27 25 23
SfoI GGC'GCC
Cuts at: 2264 3795 4112 2264
Size: 1531 317 5977
Fragments arranged by size:
5977 1531 317
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7825
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7825
SmaI CCC'GGG
Cuts at: 1872 3394 4055 4216 5152 1872
Size: 1522 661 161 936 4545
Fragments arranged by size:
4545 1522 936 661 161
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3335 4304 4789 5929 6830 7092
Size: 197 926 1392 969 485 1140 901 262
Cuts at: 7092 820
Size: 1553
Fragments arranged by size:
1553 1392 1140 969 926 901 485 262
197
SnaBI TAC'GTA
Cuts at: 493 5616 493
Size: 5123 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7825
SphI G_CATG'C
Cuts at: 1132 3578 4208 4611 5183 1132
Size: 2446 630 403 572 3774
Fragments arranged by size:
3774 2446 630 572 403
SspI AAT'ATT
Cuts at: 5 52 5749 5
Size: 47 5697 2081
Fragments arranged by size:
5697 2081 47
StuI AGG'CCT
Cuts at: 1517 3956 4118 1517
Size: 2439 162 5224
Fragments arranged by size:
5224 2439 162
StyI C'CwwG_G
Cuts at: 513 1065 2004 3601 4444 4590 4770 4842 4996
Size: 552 939 1597 843 146 180 72 154
Cuts at: 4996 5264 513
Size: 268 3074
Fragments arranged by size:
3074 1597 939 843 552 268 180 154
146 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2406 2888 3212 4400 4506 4727 4763
Size: 1255 826 482 324 1188 106 221 36
Cuts at: 4763 4820 5852 5869 6022 6207 6652 325
Size: 57 1032 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1188 1032 826 482 445 324
221 185 153 106 57 36 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3312 6071 7783
Size: 274 80 33 51 1443 1335 2759 1712
Cuts at: 7783 96
Size: 138
Fragments arranged by size:
2759 1712 1443 1335 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4148 4180 4656 4688 7785 7817 4148
Size: 32 476 32 3097 32 4156
Fragments arranged by size:
4156 3097 476 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2276 3122 3615 1316
Size: 960 846 493 5526
Fragments arranged by size:
5526 960 846 493
XbaI T'CTAG_A
Cuts at: 5077 5161 5077
Size: 84 7741
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2266 2833 2266
Size: 567 7258
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7825
XmaI C'CCGG_G
Cuts at: 1870 3392 4053 4214 5150 1870
Size: 1522 661 161 936 4545
Fragments arranged by size:
4545 1522 936 661 161
XmnI GAAnn'nnTTC
Cuts at: 3807 5069 5954 3807
Size: 1262 885 5678
Fragments arranged by size:
5678 1262 885
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5633 276
Size: 53 83 186 936 4099 2468
Fragments arranged by size:
4099 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BfrBI BsiWI BstXI BstZ17I
EcoRV FalI FseI FspAI NruI NsiI PacI PspXI
PsrI SexAI SfiI SrfI SwaI