Restriction map for
pFN21AE0395
(Circular) (Six-base) MAPSORT of: pFN21AE0395.seq Check: 875 from: 1 to: 8269
pFN21AE0395 8269 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4677 4677
Size: 8269
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6079 278
Size: 53 83 186 936 4543 2468
Fragments arranged by size:
4543 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3540 5612 1937
Size: 1603 2072 4594
Fragments arranged by size:
4594 2072 1603
Acc65I G'GTAC_C
Cuts at: 2562 5590 2562
Size: 3028 5241
AclI AA'CG_TT
Cuts at: 1563 6396 6769 1563
Size: 4833 373 3063
Fragments arranged by size:
4833 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2456 4430 6332 7716 7984 1632
Size: 824 1974 1902 1384 268 1917
Fragments arranged by size:
1974 1917 1902 1384 824 268
AfeI AGC'GCT
Cuts at: 3172 3172
Size: 8269
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8072
AflIII A'CryG_T
Cuts at: 1203 3969 4584 7168 8145 1203
Size: 2766 615 2584 977 1327
Fragments arranged by size:
2766 2584 1327 977 615
AgeI A'CCGG_T
Cuts at: 7100 7100
Size: 8269
AhdI GACnn_n'nnGTC
Cuts at: 4681 6998 4681
Size: 2317 5952
AleI CACnn'nnGTG
Cuts at: 1166 2023 1166
Size: 857 7412
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8269
AlwNI CAG_nnn'CTG
Cuts at: 2671 5359 5422 6012 7584 2671
Size: 2688 63 590 1572 3356
Fragments arranged by size:
3356 2688 1572 590 63
ApaI G_GGCC'C
Cuts at: 3618 3999 4459 4797 3618
Size: 381 460 338 7090
Fragments arranged by size:
7090 460 381 338
ApaLI G'TGCA_C
Cuts at: 5549 6326 7482 5549
Size: 777 1156 6336
Fragments arranged by size:
6336 1156 777
ApoI r'AATT_y
Cuts at: 1493 2106 3509 4419 4957 5391 5410 5578 5804
Size: 613 1403 910 538 434 19 168 226
Cuts at: 5804 5956 8065 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1403 910 613 538 434 226
168 152 19
AseI AT'TA_AT
Cuts at: 160 1032 3206 6823 160
Size: 872 2174 3617 1606
Fragments arranged by size:
3617 2174 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8269
AvaI C'yCGr_G
Cuts at: 1870 1943 5088 5269 5594 1870
Size: 73 3145 181 325 4545
Fragments arranged by size:
4545 3145 325 181 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3300 3333 3300
Size: 33 8236
BamHI G'GATC_C
Cuts at: 2152 5599 5966 2152
Size: 3447 367 4455
Fragments arranged by size:
4455 3447 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2562 5590 7045 618
Size: 325 217 627 775 3028 1455 1842
Fragments arranged by size:
3028 1842 1455 775 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2013 2175 3618 3761 3999 4459 4797 5012
Size: 622 162 1443 143 238 460 338 215
Cuts at: 5012 5588 1391
Size: 576 4072
Fragments arranged by size:
4072 1443 622 576 460 338 238 215
162 143
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2250 2331 928
Size: 971 351 81 6866
Fragments arranged by size:
6866 971 351 81
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2717 2751 6460 6494 1903
Size: 34 780 34 3709 34 3678
Fragments arranged by size:
3709 3678 780 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6161 7371 6161
Size: 1210 7059
BclI T'GATC_A
Cuts at: 1548 4152 4711 1548
Size: 2604 559 5106
Fragments arranged by size:
5106 2604 559
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6880 136
Size: 107 122 71 631 605 5208 1525
Fragments arranged by size:
5208 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4723 8264 4723
Size: 3541 4728
BlpI GC'TnA_GC
Cuts at: 5686 5686
Size: 8269
Bme1580I G_kGCm'C
Cuts at: 1790 3618 3712 3999 4459 4797 5553 6330 7486
Size: 1828 94 287 460 338 756 777 1156
Cuts at: 7486 1790
Size: 2573
Fragments arranged by size:
2573 1828 1156 777 756 460 338 287
94
BmgBI CAC'GTC
Cuts at: 1318 4267 4659 1318
Size: 2949 392 4928
Fragments arranged by size:
4928 2949 392
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2545 3098 4002
Size: 416 53 83 399 24 1128 553 904
Cuts at: 4002 4813 5550 6000 6958 442
Size: 811 737 450 958 1753
Fragments arranged by size:
1753 1128 958 904 811 737 553 450
416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8269
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2032 5158 2032
Size: 3126 5143
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2006 2159 4102 4973 5072 5266 6929 1239
Size: 767 153 1943 871 99 194 1663 2579
Fragments arranged by size:
2579 1943 1663 871 767 194 153 99
Bpu10I CC'TnA_GC
Cuts at: 3459 3459
Size: 8269
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2471 3914 5499 6394 7259 7557 2471
Size: 1443 1585 895 865 298 3183
Fragments arranged by size:
3183 1585 1443 895 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5128 6932 882
Size: 617 3629 1804 2219
Fragments arranged by size:
3629 2219 1804 617
BsaAI yAC'GTr
Cuts at: 493 1204 6060 493
Size: 711 4856 2702
Fragments arranged by size:
4856 2702 711
BsaBI GATnn'nnATC
Cuts at: 8263 8263
Size: 8269
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6076 6458 7093 275
Size: 53 83 186 936 4543 382 635 1451
Fragments arranged by size:
4543 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4998 6702 7100 7374 7521 7932 4998
Size: 1704 398 274 147 411 5335
Fragments arranged by size:
5335 1704 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3612 3642 5248 5278 1178
Size: 30 165 30 2209 30 1606 30 4169
Fragments arranged by size:
4169 2209 1606 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3611 3630 5215 1186
Size: 2425 19 1585 4240
Fragments arranged by size:
4240 2425 1585 19
BseYI C'CCAG_C
Cuts at: 3469 3791 3901 5294 5535 7472 7968 3469
Size: 322 110 1393 241 1937 496 3770
Fragments arranged by size:
3770 1937 1393 496 322 241 110
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2974 3865 4649 4979 1163
Size: 573 1238 891 784 330 4453
Fragments arranged by size:
4453 1238 891 784 573 330
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5036 5719 6480 7508 1540
Size: 275 186 3035 683 761 1028 2301
Fragments arranged by size:
3035 2301 1028 761 683 275 186
BsiHKAI G_wGCw'C
Cuts at: 2175 3167 3640 4542 5553 6330 6415 7486 2175
Size: 992 473 902 1011 777 85 1071 2958
Fragments arranged by size:
2958 1071 1011 992 902 777 473 85
BsmI GAATG_Cn'
Cuts at: 5185 5780 5873 5185
Size: 595 93 7581
Fragments arranged by size:
7581 595 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3827 3827
Size: 8269
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2175 3167 3618 3640 3712 3761
Size: 399 223 162 992 451 22 72 49
Cuts at: 3761 3999 4459 4542 4797 5012 5553 5588 6330
Size: 238 460 83 255 215 541 35 742
Cuts at: 6330 6415 7486 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 992 742 541 460 451 399
255 238 223 215 162 85 83 72
49 35 22
BspEI T'CCGG_A
Cuts at: 7932 7932
Size: 8269
BspHI T'CATG_A
Cuts at: 6158 7888 6158
Size: 1730 6539
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4677 5624 844
Size: 3833 947 3489
Fragments arranged by size:
3833 3489 947
BsrBI CCG'CTC
Cuts at: 1394 1823 2568 6156 1394
Size: 429 745 3588 3507
Fragments arranged by size:
3588 3507 745 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2289 2640 3870 6764 6938 65
Size: 1183 1041 351 1230 2894 174 1396
Fragments arranged by size:
2894 1396 1230 1183 1041 351 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2202 2559 4245 4757 6913 7100 1700
Size: 110 392 357 1686 512 2156 187 2869
Fragments arranged by size:
2869 2156 1686 512 392 357 187 110
BsrGI T'GTAC_A
Cuts at: 96 4587 5424 96
Size: 4491 837 2941
Fragments arranged by size:
4491 2941 837
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8269
BssSI C'ACGA_G
Cuts at: 6329 7341 6329
Size: 1012 7257
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8269
BstBI TT'CG_AA
Cuts at: 7073 7073
Size: 8269
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8269
BstXI CCAn_nnnn'nTGG
Cuts at: 4103 4103
Size: 8269
BstYI r'GATC_y
Cuts at: 1972 2152 2667 4348 4723 5599 5966 6351 6368
Size: 180 515 1681 375 876 367 385 17
Cuts at: 6368 7820 7906 7918 8264 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1681 1452 876 515 385 375 367
346 180 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2272 3858 4012 4213 513
Size: 552 939 268 1586 154 201 4569
Fragments arranged by size:
4569 1586 939 552 268 201 154
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2742 487
Size: 683 56 1516 6014
Fragments arranged by size:
6014 1516 683 56
BtsI GCAGTG_nn'
Cuts at: 2652 3913 5100 5785 6579 6599 2652
Size: 1261 1187 685 794 20 4322
Fragments arranged by size:
4322 1261 1187 794 685 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8269
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4131 4166 529
Size: 35 3567 35 4632
Fragments arranged by size:
4632 3567 35 35
DraI TTT'AAA
Cuts at: 3046 5573 3046
Size: 2527 5742
DraIII CAC_nnn'GTG
Cuts at: 2042 4945 2042
Size: 2903 5366
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4403 7276 809
Size: 507 3087 2873 1802
Fragments arranged by size:
3087 2873 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4074 4216 4247
Size: 54 1425 50 275 18 2244 142 31
Cuts at: 4247 5033 5716 6605 8234 8
Size: 786 683 889 1629 43
Fragments arranged by size:
2244 1629 1425 889 786 683 275 142
54 50 43 31 18
EagI C'GGCC_G
Cuts at: 1537 1812 5033 5716 1537
Size: 275 3221 683 4090
Fragments arranged by size:
4090 3221 683 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2184 2782 2943 4290 6199 1353
Size: 831 598 161 1347 1909 3423
Fragments arranged by size:
3423 1909 1347 831 598 161
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5211 6856 7230 7376 387
Size: 1064 138 3622 1645 374 146 1280
Fragments arranged by size:
3622 1645 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2173 2173
Size: 8269
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2006 2159 2456 4102 4430 4973 5072
Size: 393 374 153 297 1646 328 543 99
Cuts at: 5072 5266 6332 6929 7716 7984 1239
Size: 194 1066 597 787 268 1524
Fragments arranged by size:
1646 1524 1066 787 597 543 393 374
328 297 268 194 153 99
EcoNI CCTnn'n_nnAGG
Cuts at: 2407 2507 2407
Size: 100 8169
EcoO109I rG'GnC_Cy
Cuts at: 3614 3946 4455 4456 4765 4793 4794 4895 5470
Size: 332 509 1 309 28 1 101 575
Cuts at: 5470 3614
Size: 6413
Fragments arranged by size:
6413 575 509 332 309 101 28 1
1
EcoRI G'AATT_C
Cuts at: 5578 5956 5578
Size: 378 7891
FspI TGC'GCA
Cuts at: 6775 6775
Size: 8269
HaeII r_GCGC'y
Cuts at: 1678 3174 7416 1678
Size: 1496 4242 2531
Fragments arranged by size:
4242 2531 1496
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2582 2614 2852 2884 3392
Size: 32 135 32 1010 32 238 32 508
Cuts at: 3392 3424 4484 4516 4667 4699 5398 5430 6910
Size: 32 1060 32 151 32 699 32 1480
Cuts at: 6910 6942 6984 7016 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1480 1060 1010 699 508 238 151
135 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2451 5613 5859 669
Size: 988 281 513 3162 246 3079
Fragments arranged by size:
3162 3079 988 513 281 246
HindIII A'AGCT_T
Cuts at: 748 2318 4085 5213 748
Size: 1570 1767 1128 3804
Fragments arranged by size:
3804 1767 1570 1128
HpaI GTT'AAC
Cuts at: 5859 5859
Size: 8269
KpnI G_GTAC'C
Cuts at: 2566 5594 2566
Size: 3028 5241
MfeI C'AATT_G
Cuts at: 5868 5868
Size: 8269
MluI A'CGCG_T
Cuts at: 8145 8145
Size: 8269
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2841 3600 7383 7567 1305
Size: 1536 759 3783 184 2007
Fragments arranged by size:
3783 2007 1536 759 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4076 4218 10
Size: 54 1425 343 2244 142 4061
Fragments arranged by size:
4061 2244 1425 343 142 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2023 2645 3135 4886 6227 6586 6745
Size: 648 857 622 490 1751 1341 359 159
Cuts at: 6745 518
Size: 2042
Fragments arranged by size:
2042 1751 1341 857 648 622 490 359
159
MspA1I CmG'CkG
Cuts at: 1606 2179 3817 3901 4359 5422 5539 5685 6362
Size: 573 1638 84 458 1063 117 146 677
Cuts at: 6362 7510 7755 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1638 1148 1063 677 573 458 245
146 117 84
NaeI GCC'GGC
Cuts at: 2204 4247 2204
Size: 2043 6226
NcoI C'CATG_G
Cuts at: 513 1065 2004 2272 3858 4012 4213 513
Size: 552 939 268 1586 154 201 4569
Fragments arranged by size:
4569 1586 939 552 268 201 154
NdeI CA'TA_TG
Cuts at: 387 3217 4233 5464 387
Size: 2830 1016 1231 3192
Fragments arranged by size:
3192 2830 1231 1016
NgoMIV G'CCGG_C
Cuts at: 2202 4245 2202
Size: 2043 6226
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8269
NotI GC'GGCC_GC
Cuts at: 5716 5716
Size: 8269
NspI r_CATG'y
Cuts at: 1132 1229 3973 4477 4588 5627 7172 1132
Size: 97 2744 504 111 1039 1545 2229
Fragments arranged by size:
2744 2229 1545 1039 504 111 97
PasI CC'CwG_GG
Cuts at: 1348 2254 2632 3674 1348
Size: 906 378 1042 5943
Fragments arranged by size:
5943 1042 906 378
PciI A'CATG_T
Cuts at: 3969 4584 7168 3969
Size: 615 2584 5070
Fragments arranged by size:
5070 2584 615
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8269
PfoI T'CCnGG_A
Cuts at: 2586 5252 2586
Size: 2666 5603
PmeI GTTT'AAAC
Cuts at: 5573 5573
Size: 8269
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6338 6370 7851 7883 6338
Size: 32 1481 32 6724
Fragments arranged by size:
6724 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3946 4765 5470 3946
Size: 819 705 6745
Fragments arranged by size:
6745 819 705
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8269
PsiI TTA'TAA
Cuts at: 2991 5839 2991
Size: 2848 5421
PspOMI G'GGCC_C
Cuts at: 3614 3995 4455 4793 3614
Size: 381 460 338 7090
Fragments arranged by size:
7090 460 381 338
PstI C_TGCA'G
Cuts at: 830 4364 5316 5621 830
Size: 3534 952 305 3478
Fragments arranged by size:
3534 3478 952 305
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8269
PvuII CAG'CTG
Cuts at: 2179 3901 5422 5539 2179
Size: 1722 1521 117 4909
Fragments arranged by size:
4909 1722 1521 117
SacI G_AGCT'C
Cuts at: 2175 2175
Size: 8269
SalI G'TCGA_C
Cuts at: 1936 5611 1936
Size: 3675 4594
SbfI CC_TGCA'GG
Cuts at: 5621 5621
Size: 8269
ScaI AGT'ACT
Cuts at: 2555 5318 6517 8229 2555
Size: 2763 1199 1712 2595
Fragments arranged by size:
2763 2595 1712 1199
SexAI A'CCwGG_T
Cuts at: 2328 2328
Size: 8269
SfcI C'TryA_G
Cuts at: 826 1045 4360 4525 4719 5312 5451 5617 6752
Size: 219 3315 165 194 593 139 166 1135
Cuts at: 6752 7433 7624 826
Size: 681 191 1471
Fragments arranged by size:
3315 1471 1135 681 593 219 194 191
166 165 139
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8269
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8269
SmaI CCC'GGG
Cuts at: 1872 5090 5596 1872
Size: 3218 506 4545
Fragments arranged by size:
4545 3218 506
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2486 3929 5514 6373 7274 7536
Size: 197 926 543 1443 1585 859 901 262
Cuts at: 7536 820
Size: 1553
Fragments arranged by size:
1585 1553 1443 926 901 859 543 262
197
SnaBI TAC'GTA
Cuts at: 493 6060 493
Size: 5567 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8269
SphI G_CATG'C
Cuts at: 1132 5627 1132
Size: 4495 3774
SrfI GCCC'GGGC
Cuts at: 5090 5090
Size: 8269
SspI AAT'ATT
Cuts at: 5 52 6193 5
Size: 47 6141 2081
Fragments arranged by size:
6141 2081 47
StuI AGG'CCT
Cuts at: 1517 2402 5141 1517
Size: 885 2739 4645
Fragments arranged by size:
4645 2739 885
StyI C'CwwG_G
Cuts at: 513 1065 2004 2116 2272 2403 2847 3568 3666
Size: 552 939 112 156 131 444 721 98
Cuts at: 3666 3858 4012 4213 5115 5708 513
Size: 192 154 201 902 593 3074
Fragments arranged by size:
3074 939 902 721 593 552 444 201
192 156 154 131 112 98
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4894 5001 6296 6313 6466 6651 7096
Size: 1255 3314 107 1295 17 153 185 445
Cuts at: 7096 325
Size: 1498
Fragments arranged by size:
3314 1498 1295 1255 445 185 153 107
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2439 2553 2673
Size: 274 80 33 51 1443 462 114 120
Cuts at: 2673 4587 5316 5424 5558 6515 8227 96
Size: 1914 729 108 134 957 1712 138
Fragments arranged by size:
1914 1712 1443 957 729 462 274 138
134 120 114 108 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3612 3644 4172 4204 8229 8261 3612
Size: 32 528 32 4025 32 3620
Fragments arranged by size:
4025 3620 528 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2330 4888 1316
Size: 1014 2558 4697
Fragments arranged by size:
4697 2558 1014
XbaI T'CTAG_A
Cuts at: 5605 5605
Size: 8269
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4211 5563 4211
Size: 1352 6917
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8269
XmaI C'CCGG_G
Cuts at: 1870 5088 5594 1870
Size: 3218 506 4545
Fragments arranged by size:
4545 3218 506
XmnI GAAnn'nnTTC
Cuts at: 2791 6398 2791
Size: 3607 4662
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6077 276
Size: 53 83 186 936 4543 2468
Fragments arranged by size:
4543 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BbeI BbvCI BfrBI BsiWI BstZ17I
Bsu36I EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI
SapI SfiI SfoI SwaI