Restriction map for
pFN21AE0392
(Circular) (Six-base) MAPSORT of: pFN21AE0392.seq Check: 260 from: 1 to: 7498
pFN21AE0392 7498 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4193 4742 4193
Size: 549 6949
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5308 278
Size: 53 83 186 936 3772 2468
Fragments arranged by size:
3772 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3302 4686 4841 1937
Size: 1365 1384 155 4594
Fragments arranged by size:
4594 1384 1365 155
Acc65I G'GTAC_C
Cuts at: 4819 4819
Size: 7498
AclI AA'CG_TT
Cuts at: 1563 2766 5625 5998 1563
Size: 1203 2859 373 3063
Fragments arranged by size:
3063 2859 1203 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2490 4029 4206 4406 5561 6945 7213 1632
Size: 858 1539 177 200 1155 1384 268 1917
Fragments arranged by size:
1917 1539 1384 1155 858 268 200 177
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7301
AflIII A'CryG_T
Cuts at: 1203 3152 6397 7374 1203
Size: 1949 3245 977 1327
Fragments arranged by size:
3245 1949 1327 977
AgeI A'CCGG_T
Cuts at: 6329 6329
Size: 7498
AhdI GACnn_n'nnGTC
Cuts at: 6227 6227
Size: 7498
AleI CACnn'nnGTG
Cuts at: 1166 2751 3725 1166
Size: 1585 974 4939
Fragments arranged by size:
4939 1585 974
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7498
AlwNI CAG_nnn'CTG
Cuts at: 3595 5241 6813 3595
Size: 1646 1572 4280
Fragments arranged by size:
4280 1646 1572
ApaI G_GGCC'C
Cuts at: 3898 3898
Size: 7498
ApaLI G'TGCA_C
Cuts at: 3498 5555 6711 3498
Size: 2057 1156 4285
Fragments arranged by size:
4285 2057 1156
ApoI r'AATT_y
Cuts at: 1493 2887 4807 5033 5185 7294 1493
Size: 1394 1920 226 152 2109 1697
Fragments arranged by size:
2109 1920 1697 1394 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6052 160
Size: 872 5020 1606
Fragments arranged by size:
5020 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7498
AvaI C'yCGr_G
Cuts at: 1870 1943 2172 2738 3088 4063 4823 1870
Size: 73 229 566 350 975 760 4545
Fragments arranged by size:
4545 975 760 566 350 229 73
BamHI G'GATC_C
Cuts at: 3540 4828 5195 3540
Size: 1288 367 5843
Fragments arranged by size:
5843 1288 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2011 2167 2249 3736 4475
Size: 325 217 627 224 156 82 1487 739
Cuts at: 4475 4508 4607 4613 4819 6274 618
Size: 33 99 6 206 1455 1842
Fragments arranged by size:
1842 1487 1455 739 627 325 224 217
206 156 99 82 33 6
BanII G_rGCy'C
Cuts at: 1391 2289 3898 4592 4817 1391
Size: 898 1609 694 225 4072
Fragments arranged by size:
4072 1609 898 694 225
BbeI G_GCGC'C
Cuts at: 2015 2171 2015
Size: 156 7342
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3489 4105 928
Size: 971 1590 616 4321
Fragments arranged by size:
4321 1590 971 616
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5689 5723 1903
Size: 34 3752 34 3678
Fragments arranged by size:
3752 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2698 5390 6600 2698
Size: 2692 1210 3596
Fragments arranged by size:
3596 2692 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7498
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 6109 136
Size: 107 122 71 631 605 337 4100 1525
Fragments arranged by size:
4100 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 2049 2297 7493 2049
Size: 248 5196 2054
Fragments arranged by size:
5196 2054 248
BlpI GC'TnA_GC
Cuts at: 4915 4915
Size: 7498
Bme1580I G_kGCm'C
Cuts at: 1790 3502 3898 4668 5559 6715 1790
Size: 1712 396 770 891 1156 2573
Fragments arranged by size:
2573 1712 1156 891 770 396
BmgBI CAC'GTC
Cuts at: 1318 2548 2833 1318
Size: 1230 285 5983
Fragments arranged by size:
5983 1230 285
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2391 4639 5229
Size: 416 53 83 399 24 974 2248 590
Cuts at: 5229 6187 442
Size: 958 1753
Fragments arranged by size:
2248 1753 974 958 590 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 4142 1056
Size: 3086 4412
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3932 3932
Size: 7498
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2030 2191 2342 6158 1239
Size: 791 161 151 3816 2579
Fragments arranged by size:
3816 2579 791 161 151
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3417 3815 3902 4022 4130 5623 6488 6786 3417
Size: 398 87 120 108 1493 865 298 4129
Fragments arranged by size:
4129 1493 865 398 298 120 108 87
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2987 3576 3612 4279 4404 4462 6161
Size: 617 1488 589 36 667 125 58 1699
Cuts at: 6161 882
Size: 2219
Fragments arranged by size:
2219 1699 1488 667 617 589 125 58
36
BsaAI yAC'GTr
Cuts at: 493 1204 2749 4663 5289 493
Size: 711 1545 1914 626 2702
Fragments arranged by size:
2702 1914 1545 711 626
BsaBI GATnn'nnATC
Cuts at: 3335 7492 3335
Size: 4157 3341
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2012 2168 4562 5305
Size: 53 83 186 936 479 156 2394 743
Cuts at: 5305 5687 6322 275
Size: 382 635 1451
Fragments arranged by size:
2394 1451 936 743 635 479 382 186
156 83 53
BsaWI w'CCGG_w
Cuts at: 2482 2847 5931 6329 6603 6750 7161 2482
Size: 365 3084 398 274 147 411 2819
Fragments arranged by size:
3084 2819 411 398 365 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4259 4289 1178
Size: 30 165 30 2856 30 4387
Fragments arranged by size:
4387 2856 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2129 2297 3282 3420 1186
Size: 943 168 985 138 5264
Fragments arranged by size:
5264 985 943 168 138
BseYI C'CCAG_C
Cuts at: 4322 4747 6701 7197 4322
Size: 425 1954 496 4623
Fragments arranged by size:
4623 1954 496 425
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3851 1163
Size: 573 2115 4810
Fragments arranged by size:
4810 2115 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4948 5709 6737 1540
Size: 275 186 2947 761 1028 2301
Fragments arranged by size:
2947 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2289 3502 4592 5559 5644 6715 2289
Size: 1213 1090 967 85 1071 3072
Fragments arranged by size:
3072 1213 1090 1071 967 85
BsmI GAATG_Cn'
Cuts at: 3811 5009 5102 3811
Size: 1198 93 6207
Fragments arranged by size:
6207 1198 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2209 2823 3903 4147 2209
Size: 614 1080 244 5560
Fragments arranged by size:
5560 1080 614 244
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2289 3502 3898 4592 4668 4817 5559
Size: 399 499 1213 396 694 76 149 742
Cuts at: 5559 5644 6715 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1213 1071 742 694 499 399 396
149 85 76
BspEI T'CCGG_A
Cuts at: 2482 2847 7161 2482
Size: 365 4314 2819
Fragments arranged by size:
4314 2819 365
BspHI T'CATG_A
Cuts at: 5387 7117 5387
Size: 1730 5768
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3594 4185 4193 4742 4853 844
Size: 2750 591 8 549 111 3489
Fragments arranged by size:
3489 2750 591 549 111 8
BsrBI CCG'CTC
Cuts at: 1394 1823 3744 5385 1394
Size: 429 1921 1641 3507
Fragments arranged by size:
3507 1921 1641 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3643 4708 4765 5993 6167 65
Size: 1183 2395 1065 57 1228 174 1396
Fragments arranged by size:
2395 1396 1228 1183 1065 174 57
BsrFI r'CCGG_y
Cuts at: 1700 1810 4505 4610 6142 6329 1700
Size: 110 2695 105 1532 187 2869
Fragments arranged by size:
2869 2695 1532 187 110 105
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7498
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7498
BssSI C'ACGA_G
Cuts at: 4082 4268 5558 6570 4082
Size: 186 1290 1012 5010
Fragments arranged by size:
5010 1290 1012 186
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4663 794
Size: 3869 3629
BstBI TT'CG_AA
Cuts at: 6302 6302
Size: 7498
BstEII G'GTnAC_C
Cuts at: 1184 2257 2510 2657 3766 1184
Size: 1073 253 147 1109 4916
Fragments arranged by size:
4916 1109 1073 253 147
BstXI CCAn_nnnn'nTGG
Cuts at: 2158 3727 3778 3950 4558 2158
Size: 1569 51 172 608 5098
Fragments arranged by size:
5098 1569 608 172 51
BstYI r'GATC_y
Cuts at: 1972 2049 2297 3540 3687 4828 5195 5580 5597
Size: 77 248 1243 147 1141 367 385 17
Cuts at: 5597 7049 7135 7147 7493 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1243 1141 385 367 346 248
147 86 77 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2262 3949 4284 4617 513
Size: 552 939 258 1687 335 333 3394
Fragments arranged by size:
3394 1687 939 552 335 333 258
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2131 4512 487
Size: 683 56 905 2381 3473
Fragments arranged by size:
3473 2381 905 683 56
BtsI GCAGTG_nn'
Cuts at: 2676 3320 3789 3864 4098 5014 5808 5828 2676
Size: 644 469 75 234 916 794 20 4346
Fragments arranged by size:
4346 916 794 644 469 234 75 20
ClaI AT'CG_AT
Cuts at: 1554 2376 1554
Size: 822 6676
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7463
DraI TTT'AAA
Cuts at: 4802 4802
Size: 7498
DraIII CAC_nnn'GTG
Cuts at: 4225 4225
Size: 7498
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6505 809
Size: 507 5189 1802
Fragments arranged by size:
5189 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3477 3952 3969
Size: 54 1425 50 275 18 1647 475 17
Cuts at: 3969 3996 4095 4945 5834 7463 8
Size: 27 99 850 889 1629 43
Fragments arranged by size:
1647 1629 1425 889 850 475 275 99
54 50 43 27 18 17
EagI C'GGCC_G
Cuts at: 1537 1812 4945 1537
Size: 275 3133 4090
Fragments arranged by size:
4090 3133 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2469 2610 4292 4380 5428 1353
Size: 1116 141 1682 88 1048 3423
Fragments arranged by size:
3423 1682 1116 1048 141 88
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2198 4555 6085 6459 6605 387
Size: 1064 138 609 2357 1530 374 146 1280
Fragments arranged by size:
2357 1530 1280 1064 609 374 146 138
EcoICRI GAG'CTC
Cuts at: 2287 4590 2287
Size: 2303 5195
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2030 2191 2342 2490 4029 4206 4406
Size: 393 398 161 151 148 1539 177 200
Cuts at: 4406 5561 6158 6945 7213 1239
Size: 1155 597 787 268 1524
Fragments arranged by size:
1539 1524 1155 787 597 398 393 268
200 177 161 151 148
EcoO109I rG'GnC_Cy
Cuts at: 2155 3895 4744 2155
Size: 1740 849 4909
Fragments arranged by size:
4909 1740 849
EcoRI G'AATT_C
Cuts at: 2887 4807 5185 2887
Size: 1920 378 5200
Fragments arranged by size:
5200 1920 378
FspI TGC'GCA
Cuts at: 3884 6004 3884
Size: 2120 5378
FspAI rTGC'GCAy
Cuts at: 3884 3884
Size: 7498
HaeII r_GCGC'y
Cuts at: 1678 2015 2171 4632 6645 1678
Size: 337 156 2461 2013 2531
Fragments arranged by size:
2531 2461 2013 337 156
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2184 2216 2852 2884 3113
Size: 32 135 32 612 32 636 32 229
Cuts at: 3113 3145 3399 3431 3779 3811 4574 4606 6139
Size: 32 254 32 348 32 763 32 1533
Cuts at: 6139 6171 6213 6245 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1533 763 636 612 348 254 229
135 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3219 4842 5088 669
Size: 988 281 1281 1623 246 3079
Fragments arranged by size:
3079 1623 1281 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2472 748
Size: 1724 5774
HpaI GTT'AAC
Cuts at: 5088 5088
Size: 7498
KasI G'GCGC_C
Cuts at: 2011 2167 2011
Size: 156 7342
KpnI G_GTAC'C
Cuts at: 4823 4823
Size: 7498
MfeI C'AATT_G
Cuts at: 5097 5097
Size: 7498
MluI A'CGCG_T
Cuts at: 7374 7374
Size: 7498
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6612 6796 1305
Size: 5307 184 2007
Fragments arranged by size:
5307 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3954 10
Size: 54 1425 343 2122 3554
Fragments arranged by size:
3554 2122 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2751 3123 3725 4538 5456 5815 5974
Size: 648 1585 372 602 813 918 359 159
Cuts at: 5974 518
Size: 2042
Fragments arranged by size:
2042 1585 918 813 648 602 372 359
159
MspA1I CmG'CkG
Cuts at: 1606 2247 2264 3592 4100 4286 4482 4724 4914
Size: 641 17 1328 508 186 196 242 190
Cuts at: 4914 5591 6739 6984 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1328 1148 677 641 508 245 242
196 190 186 17
NaeI GCC'GGC
Cuts at: 4612 4612
Size: 7498
NarI GG'CG_CC
Cuts at: 2012 2168 2012
Size: 156 7342
NcoI C'CATG_G
Cuts at: 513 1065 2004 3949 4617 513
Size: 552 939 1945 668 3394
Fragments arranged by size:
3394 1945 939 668 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7498
NgoMIV G'CCGG_C
Cuts at: 4610 4610
Size: 7498
NheI G'CTAG_C
Cuts at: 1052 4138 1052
Size: 3086 4412
NotI GC'GGCC_GC
Cuts at: 4945 4945
Size: 7498
NruI TCG'CGA
Cuts at: 2610 2610
Size: 7498
NspI r_CATG'y
Cuts at: 1132 1229 3156 4856 6401 1132
Size: 97 1927 1700 1545 2229
Fragments arranged by size:
2229 1927 1700 1545 97
PasI CC'CwG_GG
Cuts at: 1348 4597 1348
Size: 3249 4249
PciI A'CATG_T
Cuts at: 3152 6397 3152
Size: 3245 4253
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4754 1108
Size: 3646 3852
PfoI T'CCnGG_A
Cuts at: 3072 3942 3072
Size: 870 6628
PmeI GTTT'AAAC
Cuts at: 4802 4802
Size: 7498
PmlI CAC'GTG
Cuts at: 4663 4663
Size: 7498
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2082 2114 5567 5599 7080 7112 2082
Size: 32 3453 32 1481 32 2468
Fragments arranged by size:
3453 2468 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2155 2155
Size: 7498
PshAI GACnn'nnGTC
Cuts at: 1613 3798 1613
Size: 2185 5313
PsiI TTA'TAA
Cuts at: 5068 5068
Size: 7498
PspOMI G'GGCC_C
Cuts at: 3894 3894
Size: 7498
PstI C_TGCA'G
Cuts at: 830 2508 3591 4257 4659 4850 830
Size: 1678 1083 666 402 191 3478
Fragments arranged by size:
3478 1678 1083 666 402 191
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7498
PvuII CAG'CTG
Cuts at: 3592 4724 3592
Size: 1132 6366
SacI G_AGCT'C
Cuts at: 2289 4592 2289
Size: 2303 5195
SacII CC_GC'GG
Cuts at: 2265 4287 2265
Size: 2022 5476
SalI G'TCGA_C
Cuts at: 1936 4840 1936
Size: 2904 4594
SbfI CC_TGCA'GG
Cuts at: 2508 4850 2508
Size: 2342 5156
ScaI AGT'ACT
Cuts at: 5746 7458 5746
Size: 1712 5786
SexAI A'CCwGG_T
Cuts at: 3762 3762
Size: 7498
SfcI C'TryA_G
Cuts at: 826 1045 2504 2772 3303 3587 3820 4253 4655
Size: 219 1459 268 531 284 233 433 402
Cuts at: 4655 4846 5981 6662 6853 826
Size: 191 1135 681 191 1471
Fragments arranged by size:
1471 1459 1135 681 531 433 402 284
268 233 219 191 191
SfoI GGC'GCC
Cuts at: 2013 2169 2013
Size: 156 7342
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7498
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7498
SmaI CCC'GGG
Cuts at: 1872 2174 3090 4825 1872
Size: 302 916 1735 4545
Fragments arranged by size:
4545 1735 916 302
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3432 3830 3917 4037 4145 5602
Size: 197 926 1489 398 87 120 108 1457
Cuts at: 5602 6503 6765 820
Size: 901 262 1553
Fragments arranged by size:
1553 1489 1457 926 901 398 262 197
120 108 87
SnaBI TAC'GTA
Cuts at: 493 5289 493
Size: 4796 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7498
SphI G_CATG'C
Cuts at: 1132 4856 1132
Size: 3724 3774
SspI AAT'ATT
Cuts at: 5 52 2913 5422 5
Size: 47 2861 2509 2081
Fragments arranged by size:
2861 2509 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7498
StyI C'CwwG_G
Cuts at: 513 1065 2004 2151 2253 3949 4617 4937 513
Size: 552 939 147 102 1696 668 320 3074
Fragments arranged by size:
3074 1696 939 668 552 320 147 102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2410 3367 5525 5542 5695 5880 6325
Size: 1255 830 957 2158 17 153 185 445
Cuts at: 6325 325
Size: 1498
Fragments arranged by size:
2158 1498 1255 957 830 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5744 7456 96
Size: 274 80 33 51 1443 3767 1712 138
Fragments arranged by size:
3767 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3342 3374 4259 4291 7458 7490 3342
Size: 32 885 32 3167 32 3350
Fragments arranged by size:
3350 3167 885 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3677 1316
Size: 2361 5137
XbaI T'CTAG_A
Cuts at: 2332 2602 2923 4834 2332
Size: 270 321 1911 4996
Fragments arranged by size:
4996 1911 321 270
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2975 2975
Size: 7498
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7498
XmaI C'CCGG_G
Cuts at: 1870 2172 3088 4823 1870
Size: 302 916 1735 4545
Fragments arranged by size:
4545 1735 916 302
XmnI GAAnn'nnTTC
Cuts at: 2094 5627 2094
Size: 3533 3965
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5306 276
Size: 53 83 186 936 3772 2468
Fragments arranged by size:
3772 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BstZ17I Bsu36I EcoNI EcoRV FalI FseI NsiI
PacI PspXI PsrI RsrII SanDI SapI SfiI SrfI
SwaI