Restriction map for
pFN21AE0388
(Circular) (Six-base) MAPSORT of: pFN21AE0388.seq Check: 8468 from: 1 to: 6535
pFN21AE0388 6535 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4345 278
Size: 53 83 186 936 2809 2468
Fragments arranged by size:
2809 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3878 1937
Size: 1941 4594
Acc65I G'GTAC_C
Cuts at: 3856 3856
Size: 6535
AclI AA'CG_TT
Cuts at: 1563 4662 5035 1563
Size: 3099 373 3063
Fragments arranged by size:
3099 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2520 2598 4598 5982 6250 1632
Size: 888 78 2000 1384 268 1917
Fragments arranged by size:
2000 1917 1384 888 268 78
AfeI AGC'GCT
Cuts at: 3577 3577
Size: 6535
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6338
AflIII A'CryG_T
Cuts at: 1203 2681 5434 6411 1203
Size: 1478 2753 977 1327
Fragments arranged by size:
2753 1478 1327 977
AgeI A'CCGG_T
Cuts at: 5366 5366
Size: 6535
AhdI GACnn_n'nnGTC
Cuts at: 5264 5264
Size: 6535
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6535
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6535
AlwNI CAG_nnn'CTG
Cuts at: 2854 3423 3787 4278 5850 2854
Size: 569 364 491 1572 3539
Fragments arranged by size:
3539 1572 569 491 364
ApaI G_GGCC'C
Cuts at: 2059 2074 2190 2059
Size: 15 116 6404
Fragments arranged by size:
6404 116 15
ApaLI G'TGCA_C
Cuts at: 4592 5748 4592
Size: 1156 5379
ApoI r'AATT_y
Cuts at: 1493 2323 2536 2548 2563 3129 3844 4070 4222
Size: 830 213 12 15 566 715 226 152
Cuts at: 4222 6331 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 830 715 566 226 213 152
15 12
AseI AT'TA_AT
Cuts at: 160 1032 5089 160
Size: 872 4057 1606
Fragments arranged by size:
4057 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6535
AvaI C'yCGr_G
Cuts at: 1870 1943 2050 3860 1870
Size: 73 107 1810 4545
Fragments arranged by size:
4545 1810 107 73
BamHI G'GATC_C
Cuts at: 3865 4232 3865
Size: 367 6168
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3856 5311 618
Size: 325 217 627 2069 1455 1842
Fragments arranged by size:
2069 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2059 2074 2190 3854 1391
Size: 668 15 116 1664 4072
Fragments arranged by size:
4072 1664 668 116 15
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3167 928
Size: 971 1268 4296
Fragments arranged by size:
4296 1268 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4726 4760 1903
Size: 34 50 34 2705 34 3678
Fragments arranged by size:
3678 2705 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4427 5637 4427
Size: 1210 5325
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6535
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3824 5146 136
Size: 107 122 71 631 605 2152 1322 1525
Fragments arranged by size:
2152 1525 1322 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6530 6530
Size: 6535
BlpI GC'TnA_GC
Cuts at: 3952 3952
Size: 6535
Bme1580I G_kGCm'C
Cuts at: 1790 2059 2074 2190 4596 5752 1790
Size: 269 15 116 2406 1156 2573
Fragments arranged by size:
2573 2406 1156 269 116 15
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6535
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2307 4266 5224
Size: 416 53 83 399 24 890 1959 958
Cuts at: 5224 442
Size: 1753
Fragments arranged by size:
1959 1753 958 890 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6535
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3021 3021
Size: 6535
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3457 5195 1239
Size: 2218 1738 2579
Fragments arranged by size:
2579 2218 1738
Bpu10I CC'TnA_GC
Cuts at: 2207 2207
Size: 6535
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2956 4660 5525 5823 2956
Size: 1704 865 298 3668
Fragments arranged by size:
3668 1704 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5198 882
Size: 617 3699 2219
Fragments arranged by size:
3699 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3643 4326 493
Size: 711 2439 683 2702
Fragments arranged by size:
2702 2439 711 683
BsaBI GATnn'nnATC
Cuts at: 6529 6529
Size: 6535
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4342 4724 5359 275
Size: 53 83 186 936 2809 382 635 1451
Fragments arranged by size:
2809 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4968 5366 5640 5787 6198 4968
Size: 398 274 147 411 5305
Fragments arranged by size:
5305 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2156 2186 1178
Size: 30 165 30 753 30 5527
Fragments arranged by size:
5527 753 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3779 1186
Size: 2593 3942
BseYI C'CCAG_C
Cuts at: 2023 2189 2371 3699 3747 5738 6234 2023
Size: 166 182 1328 48 1991 496 2324
Fragments arranged by size:
2324 1991 1328 496 182 166 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2315 2736 1163
Size: 573 579 421 4962
Fragments arranged by size:
4962 579 573 421
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2033 3985 4746 5774 1540
Size: 275 186 32 1952 761 1028 2301
Fragments arranged by size:
2301 1952 1028 761 275 186 32
BsiHKAI G_wGCw'C
Cuts at: 4596 4681 5752 4596
Size: 85 1071 5379
Fragments arranged by size:
5379 1071 85
BsmI GAATG_Cn'
Cuts at: 4046 4139 4046
Size: 93 6442
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2059 2074 2190 3854 4596 4681 5752
Size: 399 269 15 116 1664 742 85 1071
Cuts at: 5752 1391
Size: 2174
Fragments arranged by size:
2174 1664 1071 742 399 269 116 85
15
BspEI T'CCGG_A
Cuts at: 6198 6198
Size: 6535
BspHI T'CATG_A
Cuts at: 3618 4424 6154 3618
Size: 806 1730 3999
Fragments arranged by size:
3999 1730 806
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3890 844
Size: 3046 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4422 1394
Size: 429 2599 3507
Fragments arranged by size:
3507 2599 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2406 5030 5204 65
Size: 1183 1158 2624 174 1396
Fragments arranged by size:
2624 1396 1183 1158 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2226 5179 5366 1700
Size: 110 416 2953 187 2869
Fragments arranged by size:
2953 2869 416 187 110
BsrGI T'GTAC_A
Cuts at: 96 2656 96
Size: 2560 3975
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6535
BssSI C'ACGA_G
Cuts at: 4595 5607 4595
Size: 1012 5523
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6535
BstBI TT'CG_AA
Cuts at: 5339 5339
Size: 6535
BstEII G'GTnAC_C
Cuts at: 1184 3686 1184
Size: 2502 4033
BstXI CCAn_nnnn'nTGG
Cuts at: 3534 3534
Size: 6535
BstYI r'GATC_y
Cuts at: 1972 3073 3757 3865 4232 4617 4634 6086 6172
Size: 1101 684 108 367 385 17 1452 86
Cuts at: 6172 6184 6530 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1101 684 385 367 346 108
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2066 2899 2066
Size: 833 5702
BtgI C'CryG_G
Cuts at: 513 1065 2059 2153 2181 3049 3234 3489 513
Size: 552 994 94 28 868 185 255 3559
Fragments arranged by size:
3559 994 868 552 255 185 94 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5796
Fragments arranged by size:
5796 683 56
BtsI GCAGTG_nn'
Cuts at: 4051 4845 4865 4051
Size: 794 20 5721
Fragments arranged by size:
5721 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6535
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6500
DraI TTT'AAA
Cuts at: 2569 3839 2569
Size: 1270 5265
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5542 809
Size: 507 4226 1802
Fragments arranged by size:
4226 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2030 3381 3666
Size: 54 1425 50 275 18 200 1351 285
Cuts at: 3666 3982 4871 6500 8
Size: 316 889 1629 43
Fragments arranged by size:
1629 1425 1351 889 316 285 275 200
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2030 3982 1537
Size: 275 218 1952 4090
Fragments arranged by size:
4090 1952 275 218
EarI CTCTTCn'nnn_
Cuts at: 1353 4465 1353
Size: 3112 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5122 5496 5642 387
Size: 1064 138 3533 374 146 1280
Fragments arranged by size:
3533 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2520 2598 3457 4598 5195 5982 6250
Size: 393 888 78 859 1141 597 787 268
Cuts at: 6250 1239
Size: 1524
Fragments arranged by size:
1524 1141 888 859 787 597 393 268
78
EcoNI CCTnn'n_nnAGG
Cuts at: 2210 2210
Size: 6535
EcoO109I rG'GnC_Cy
Cuts at: 2055 2070 2071 2186 3825 2055
Size: 15 1 115 1639 4765
Fragments arranged by size:
4765 1639 115 15 1
EcoRI G'AATT_C
Cuts at: 3844 4222 3844
Size: 378 6157
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2950 2950
Size: 6535
FspI TGC'GCA
Cuts at: 5041 5041
Size: 6535
HaeII r_GCGC'y
Cuts at: 1678 2136 3579 5682 1678
Size: 458 1443 2103 2531
Fragments arranged by size:
2531 2103 1443 458
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2156 2188 2836 2868 5176
Size: 32 135 32 584 32 648 32 2308
Cuts at: 5176 5208 5250 5282 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2308 648 584 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2419 3879 4125 669
Size: 988 281 481 1460 246 3079
Fragments arranged by size:
3079 1460 988 481 281 246
HindIII A'AGCT_T
Cuts at: 748 3120 748
Size: 2372 4163
HpaI GTT'AAC
Cuts at: 4125 4125
Size: 6535
KpnI G_GTAC'C
Cuts at: 3860 3860
Size: 6535
MfeI C'AATT_G
Cuts at: 4134 4134
Size: 6535
MluI A'CGCG_T
Cuts at: 2681 6411 2681
Size: 3730 2805
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2693 3515 5649 5833 1305
Size: 1388 822 2134 184 2007
Fragments arranged by size:
2134 2007 1388 822 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3383 10
Size: 54 1425 343 1551 3162
Fragments arranged by size:
3162 1551 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4493 4852 5011 518
Size: 648 3327 359 159 2042
Fragments arranged by size:
3327 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2061 2089 2116 2155 2183 3787 3951 4628
Size: 455 28 27 39 28 1604 164 677
Cuts at: 4628 5776 6021 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1604 1148 677 455 245 164 39
28 28 27
NaeI GCC'GGC
Cuts at: 2228 2228
Size: 6535
NcoI C'CATG_G
Cuts at: 513 1065 3049 3234 3489 513
Size: 552 1984 185 255 3559
Fragments arranged by size:
3559 1984 552 255 185
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6535
NgoMIV G'CCGG_C
Cuts at: 2226 2226
Size: 6535
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6535
NotI GC'GGCC_GC
Cuts at: 3982 3982
Size: 6535
NspI r_CATG'y
Cuts at: 1132 1229 2348 2663 3893 5438 1132
Size: 97 1119 315 1230 1545 2229
Fragments arranged by size:
2229 1545 1230 1119 315 97
PasI CC'CwG_GG
Cuts at: 1348 3820 1348
Size: 2472 4063
PciI A'CATG_T
Cuts at: 5434 5434
Size: 6535
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6535
PmeI GTTT'AAAC
Cuts at: 3839 3839
Size: 6535
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4604 4636 6117 6149 4604
Size: 32 1481 32 4990
Fragments arranged by size:
4990 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6535
PsiI TTA'TAA
Cuts at: 4105 4105
Size: 6535
PspOMI G'GGCC_C
Cuts at: 2055 2070 2186 2055
Size: 15 116 6404
Fragments arranged by size:
6404 116 15
PstI C_TGCA'G
Cuts at: 830 2172 3887 830
Size: 1342 1715 3478
Fragments arranged by size:
3478 1715 1342
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6535
PvuII CAG'CTG
Cuts at: 3787 3787
Size: 6535
SacII CC_GC'GG
Cuts at: 2062 2156 2184 2062
Size: 94 28 6413
Fragments arranged by size:
6413 94 28
SalI G'TCGA_C
Cuts at: 1936 3877 1936
Size: 1941 4594
SbfI CC_TGCA'GG
Cuts at: 3887 3887
Size: 6535
ScaI AGT'ACT
Cuts at: 4783 6495 4783
Size: 1712 4823
SexAI A'CCwGG_T
Cuts at: 3336 3336
Size: 6535
SfcI C'TryA_G
Cuts at: 826 1045 2168 3883 5018 5699 5890 826
Size: 219 1123 1715 1135 681 191 1471
Fragments arranged by size:
1715 1471 1135 1123 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6535
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6535
SmaI CCC'GGG
Cuts at: 1872 2052 3862 1872
Size: 180 1810 4545
Fragments arranged by size:
4545 1810 180
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2935 4639 5540 5802 820
Size: 197 926 992 1704 901 262 1553
Fragments arranged by size:
1704 1553 992 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4326 493
Size: 3833 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6535
SphI G_CATG'C
Cuts at: 1132 2348 3893 1132
Size: 1216 1545 3774
Fragments arranged by size:
3774 1545 1216
SspI AAT'ATT
Cuts at: 5 52 2724 2742 4459 5
Size: 47 2672 18 1717 2081
Fragments arranged by size:
2672 2081 1717 47 18
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6535
StyI C'CwwG_G
Cuts at: 513 1065 2355 2619 2815 3049 3234 3489 3974
Size: 552 1290 264 196 234 185 255 485
Cuts at: 3974 513
Size: 3074
Fragments arranged by size:
3074 1290 552 485 264 255 234 196
185
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2225 2852 4562 4579 4732 4917 5362
Size: 1255 645 627 1710 17 153 185 445
Cuts at: 5362 325
Size: 1498
Fragments arranged by size:
1710 1498 1255 645 627 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2480 2656 3016
Size: 274 80 33 51 1443 503 176 360
Cuts at: 3016 4781 6493 96
Size: 1765 1712 138
Fragments arranged by size:
1765 1712 1443 503 360 274 176 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2367 2399 6495 6527 2367
Size: 32 4096 32 2375
Fragments arranged by size:
4096 2375 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6535
XbaI T'CTAG_A
Cuts at: 3871 3871
Size: 6535
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6535
XmaI C'CCGG_G
Cuts at: 1870 2050 3860 1870
Size: 180 1810 4545
Fragments arranged by size:
4545 1810 180
XmnI GAAnn'nnTTC
Cuts at: 4664 4664
Size: 6535
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4343 276
Size: 53 83 186 936 2809 2468
Fragments arranged by size:
2809 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI
KasI NarI NruI NsiI PacI PfoI PmlI PpuMI
PspXI PsrI RsrII SacI SanDI SapI SfiI SfoI
SrfI SwaI XcmI