Restriction map for
pFN21AE0387
(Circular) (Six-base) MAPSORT of: pFN21AE0387.seq Check: 2159 from: 1 to: 6514
pFN21AE0387 6514 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3743 3743
Size: 6514
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4324 278
Size: 53 83 186 936 2788 2468
Fragments arranged by size:
2788 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2176 2553 3857 1937
Size: 239 377 1304 4594
Fragments arranged by size:
4594 1304 377 239
Acc65I G'GTAC_C
Cuts at: 3835 3835
Size: 6514
AclI AA'CG_TT
Cuts at: 1563 2189 3813 4641 5014 1563
Size: 626 1624 828 373 3063
Fragments arranged by size:
3063 1624 828 626 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2237 2545 2600 4577 5961 6229 1632
Size: 605 308 55 1977 1384 268 1917
Fragments arranged by size:
1977 1917 1384 605 308 268 55
AfeI AGC'GCT
Cuts at: 2420 2420
Size: 6514
AflII C'TTAA_G
Cuts at: 820 1017 3173 820
Size: 197 2156 4161
Fragments arranged by size:
4161 2156 197
AflIII A'CryG_T
Cuts at: 1203 5413 6390 1203
Size: 4210 977 1327
Fragments arranged by size:
4210 1327 977
AgeI A'CCGG_T
Cuts at: 5345 5345
Size: 6514
AhdI GACnn_n'nnGTC
Cuts at: 5243 5243
Size: 6514
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6514
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6514
AlwNI CAG_nnn'CTG
Cuts at: 2215 3766 4257 5829 2215
Size: 1551 491 1572 2900
Fragments arranged by size:
2900 1572 1551 491
ApaLI G'TGCA_C
Cuts at: 4571 5727 4571
Size: 1156 5358
ApoI r'AATT_y
Cuts at: 1493 3347 3800 3823 4049 4201 6310 1493
Size: 1854 453 23 226 152 2109 1697
Fragments arranged by size:
2109 1854 1697 453 226 152 23
AseI AT'TA_AT
Cuts at: 160 1032 5068 160
Size: 872 4036 1606
Fragments arranged by size:
4036 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6514
AvaI C'yCGr_G
Cuts at: 1870 1943 3839 1870
Size: 73 1896 4545
Fragments arranged by size:
4545 1896 73
BamHI G'GATC_C
Cuts at: 2892 3844 4211 2892
Size: 952 367 5195
Fragments arranged by size:
5195 952 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2117 2146 2397 3835 5290
Size: 325 217 627 330 29 251 1438 1455
Cuts at: 5290 618
Size: 1842
Fragments arranged by size:
1842 1455 1438 627 330 325 251 217
29
BanII G_rGCy'C
Cuts at: 1391 2168 3369 3446 3833 1391
Size: 777 1201 77 387 4072
Fragments arranged by size:
4072 1201 777 387 77
BbeI G_GCGC'C
Cuts at: 2150 2150
Size: 6514
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2290 2601 928
Size: 971 391 311 4841
Fragments arranged by size:
4841 971 391 311
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4705 4739 1903
Size: 34 2768 34 3678
Fragments arranged by size:
3678 2768 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4406 5616 4406
Size: 1210 5304
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6514
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2684 5125 136
Size: 107 122 71 631 605 1012 2441 1525
Fragments arranged by size:
2441 1525 1012 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3024 6509 3024
Size: 3485 3029
BlpI GC'TnA_GC
Cuts at: 2256 3931 2256
Size: 1675 4839
Bme1580I G_kGCm'C
Cuts at: 1790 2795 3135 3407 4575 5731 1790
Size: 1005 340 272 1168 1156 2573
Fragments arranged by size:
2573 1168 1156 1005 340 272
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6514
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2610 2700 2881
Size: 416 53 83 399 24 1193 90 181
Cuts at: 2881 4245 5203 442
Size: 1364 958 1753
Fragments arranged by size:
1753 1364 1193 958 416 399 181 90
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6514
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2271 2271
Size: 6514
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2447 2508 2889 3085 3544 3564 5174 1239
Size: 1208 61 381 196 459 20 1610 2579
Fragments arranged by size:
2579 1610 1208 459 381 196 61 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4639 5504 5802 4639
Size: 865 298 5351
Fragments arranged by size:
5351 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3540 5177 882
Size: 617 2041 1637 2219
Fragments arranged by size:
2219 2041 1637 617
BsaAI yAC'GTr
Cuts at: 493 1204 4305 493
Size: 711 3101 2702
Fragments arranged by size:
3101 2702 711
BsaBI GATnn'nnATC
Cuts at: 2897 6508 2897
Size: 3611 2903
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2147 4321 4703 5338
Size: 53 83 186 936 614 2174 382 635
Cuts at: 5338 275
Size: 1451
Fragments arranged by size:
2174 1451 936 635 614 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2244 4947 5345 5619 5766 6177 2244
Size: 2703 398 274 147 411 2581
Fragments arranged by size:
2703 2581 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6289
Fragments arranged by size:
6289 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2477 1186
Size: 1291 5223
BseYI C'CCAG_C
Cuts at: 2671 2927 3254 3552 3756 5717 6213 2671
Size: 256 327 298 204 1961 496 2972
Fragments arranged by size:
2972 1961 496 327 298 256 204
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3438 1163
Size: 573 1702 4239
Fragments arranged by size:
4239 1702 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3150 3964 4725 5753 1540
Size: 275 186 1149 814 761 1028 2301
Fragments arranged by size:
2301 1149 1028 814 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2511 2577 4575 4660 5731 2511
Size: 66 1998 85 1071 3294
Fragments arranged by size:
3294 1998 1071 85 66
BsmI GAATG_Cn'
Cuts at: 2673 4025 4118 2673
Size: 1352 93 5069
Fragments arranged by size:
5069 1352 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2168 2511 2577 2795 3135 3369 3407
Size: 399 378 343 66 218 340 234 38
Cuts at: 3407 3446 3833 4575 4660 5731 1391
Size: 39 387 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 387 378 343 340
234 218 85 66 39 38
BspEI T'CCGG_A
Cuts at: 2244 6177 2244
Size: 3933 2581
BspHI T'CATG_A
Cuts at: 3726 4403 6133 3726
Size: 677 1730 4107
Fragments arranged by size:
4107 1730 677
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3348 3496 3743 3869 844
Size: 2504 148 247 126 3489
Fragments arranged by size:
3489 2504 247 148 126
BsrBI CCG'CTC
Cuts at: 1394 1823 2104 4401 1394
Size: 429 281 2297 3507
Fragments arranged by size:
3507 2297 429 281
BsrDI GCAATG_nn'
Cuts at: 65 1248 2763 5009 5183 65
Size: 1183 1515 2246 174 1396
Fragments arranged by size:
2246 1515 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2149 2957 3228 5158 5345 1700
Size: 110 339 808 271 1930 187 2869
Fragments arranged by size:
2869 1930 808 339 271 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6514
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6514
BssSI C'ACGA_G
Cuts at: 4574 5586 4574
Size: 1012 5502
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2849 3699 794
Size: 2055 850 3609
Fragments arranged by size:
3609 2055 850
BstBI TT'CG_AA
Cuts at: 2632 5318 2632
Size: 2686 3828
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6514
BstYI r'GATC_y
Cuts at: 1972 2892 3024 3390 3720 3844 4211 4596 4613
Size: 920 132 366 330 124 367 385 17
Cuts at: 4613 6065 6151 6163 6509 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 920 385 367 366 346 330
132 124 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2973 3360 3478 513
Size: 552 1908 387 118 3549
Fragments arranged by size:
3549 1908 552 387 118
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5775
Fragments arranged by size:
5775 683 56
BtsI GCAGTG_nn'
Cuts at: 3709 3767 4030 4824 4844 3709
Size: 58 263 794 20 5379
Fragments arranged by size:
5379 794 263 58 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6514
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6479
DraI TTT'AAA
Cuts at: 3818 3818
Size: 6514
DraIII CAC_nnn'GTG
Cuts at: 2506 2723 2506
Size: 217 6297
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5521 809
Size: 507 4205 1802
Fragments arranged by size:
4205 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2955 3147 3183
Size: 54 1425 50 275 18 1125 192 36
Cuts at: 3183 3961 4850 6479 8
Size: 778 889 1629 43
Fragments arranged by size:
1629 1425 1125 889 778 275 192 54
50 43 36 18
EagI C'GGCC_G
Cuts at: 1537 1812 3147 3961 1537
Size: 275 1335 814 4090
Fragments arranged by size:
4090 1335 814 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4444 1353
Size: 3091 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2026 5101 5475 5621 387
Size: 1064 138 437 3075 374 146 1280
Fragments arranged by size:
3075 1280 1064 437 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2237 2447 2508 2545 2600 2889 3085
Size: 393 605 210 61 37 55 289 196
Cuts at: 3085 3544 3564 4577 5174 5961 6229 1239
Size: 459 20 1013 597 787 268 1524
Fragments arranged by size:
1524 1013 787 605 597 459 393 289
268 210 196 61 55 37 20
EcoNI CCTnn'n_nnAGG
Cuts at: 3100 3100
Size: 6514
EcoO109I rG'GnC_Cy
Cuts at: 2661 2676 3142 3337 3435 2661
Size: 15 466 195 98 5740
Fragments arranged by size:
5740 466 195 98 15
EcoRI G'AATT_C
Cuts at: 3823 4201 3823
Size: 378 6136
EcoRV GAT'ATC
Cuts at: 2392 2392
Size: 6514
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3589 3589
Size: 6514
FspI TGC'GCA
Cuts at: 5020 5020
Size: 6514
HaeII r_GCGC'y
Cuts at: 1678 2045 2093 2150 2422 3229 5661 1678
Size: 367 48 57 272 807 2432 2531
Fragments arranged by size:
2531 2432 807 367 272 57 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2302 2331 2334 2351 2363
Size: 32 135 32 730 29 3 17 12
Cuts at: 2363 2383 2462 2494 3519 3551 5155 5187 5229
Size: 20 79 32 1025 32 1604 32 42
Cuts at: 5229 5261 1373
Size: 32 2626
Fragments arranged by size:
2626 1604 1025 730 135 79 42 32
32 32 32 32 32 29 20 17
12 3
HincII GTy'rAC
Cuts at: 669 1657 1938 2177 2554 2737 3858 4104 669
Size: 988 281 239 377 183 1121 246 3079
Fragments arranged by size:
3079 1121 988 377 281 246 239 183
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6514
HpaI GTT'AAC
Cuts at: 4104 4104
Size: 6514
KasI G'GCGC_C
Cuts at: 2146 2146
Size: 6514
KpnI G_GTAC'C
Cuts at: 3839 3839
Size: 6514
MfeI C'AATT_G
Cuts at: 4113 4113
Size: 6514
MluI A'CGCG_T
Cuts at: 6390 6390
Size: 6514
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5628 5812 1305
Size: 4323 184 2007
Fragments arranged by size:
4323 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4692
Fragments arranged by size:
4692 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4472 4831 4990 518
Size: 648 3306 359 159 2042
Fragments arranged by size:
3306 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3254 3640 3690 3930 4607 5755 6000 1606
Size: 1648 386 50 240 677 1148 245 2120
Fragments arranged by size:
2120 1648 1148 677 386 245 240 50
NaeI GCC'GGC
Cuts at: 2151 3230 2151
Size: 1079 5435
NarI GG'CG_CC
Cuts at: 2147 2147
Size: 6514
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5962
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6514
NgoMIV G'CCGG_C
Cuts at: 2149 3228 2149
Size: 1079 5435
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6514
NotI GC'GGCC_GC
Cuts at: 3961 3961
Size: 6514
NspI r_CATG'y
Cuts at: 1132 1229 3505 3872 5417 1132
Size: 97 2276 367 1545 2229
Fragments arranged by size:
2276 2229 1545 367 97
PasI CC'CwG_GG
Cuts at: 1348 2123 1348
Size: 775 5739
PciI A'CATG_T
Cuts at: 5413 5413
Size: 6514
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6514
PmeI GTTT'AAAC
Cuts at: 3818 3818
Size: 6514
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4583 4615 6096 6128 4583
Size: 32 1481 32 4969
Fragments arranged by size:
4969 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2661 3142 3337 2661
Size: 481 195 5838
Fragments arranged by size:
5838 481 195
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6514
PsiI TTA'TAA
Cuts at: 4084 4084
Size: 6514
PstI C_TGCA'G
Cuts at: 830 2034 2916 3379 3704 3866 830
Size: 1204 882 463 325 162 3478
Fragments arranged by size:
3478 1204 882 463 325 162
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6514
PvuII CAG'CTG
Cuts at: 3640 3690 3640
Size: 50 6464
SalI G'TCGA_C
Cuts at: 1936 2175 2552 3856 1936
Size: 239 377 1304 4594
Fragments arranged by size:
4594 1304 377 239
SbfI CC_TGCA'GG
Cuts at: 2034 3866 2034
Size: 1832 4682
ScaI AGT'ACT
Cuts at: 4762 6474 4762
Size: 1712 4802
SfcI C'TryA_G
Cuts at: 826 1045 2030 2912 3118 3375 3700 3862 4997
Size: 219 985 882 206 257 325 162 1135
Cuts at: 4997 5678 5869 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 985 882 681 325 257 219
206 191 162
SfoI GGC'GCC
Cuts at: 2148 2148
Size: 6514
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6514
SgrAI Cr'CCGG_yG
Cuts at: 1700 2149 1700
Size: 449 6065
SmaI CCC'GGG
Cuts at: 1872 3841 1872
Size: 1969 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3173 4618 5519 5781 820
Size: 197 926 1230 1445 901 262 1553
Fragments arranged by size:
1553 1445 1230 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4305 493
Size: 3812 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6514
SphI G_CATG'C
Cuts at: 1132 3872 1132
Size: 2740 3774
SspI AAT'ATT
Cuts at: 5 52 4438 5
Size: 47 4386 2081
Fragments arranged by size:
4386 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6514
StyI C'CwwG_G
Cuts at: 513 1065 3438 3953 513
Size: 552 2373 515 3074
Fragments arranged by size:
3074 2373 552 515
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2315 2415 3770 4541 4558 4711 4896
Size: 1255 735 100 1355 771 17 153 185
Cuts at: 4896 5341 325
Size: 445 1498
Fragments arranged by size:
1498 1355 1255 771 735 445 185 153
100 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2545 2880 3213
Size: 274 80 33 51 1443 568 335 333
Cuts at: 3213 4760 6472 96
Size: 1547 1712 138
Fragments arranged by size:
1712 1547 1443 568 335 333 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2969 3001 6474 6506 2969
Size: 32 3473 32 2977
Fragments arranged by size:
3473 2977 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6514
XbaI T'CTAG_A
Cuts at: 3850 3850
Size: 6514
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3251 3680 3251
Size: 429 6085
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6514
XmaI C'CCGG_G
Cuts at: 1870 3839 1870
Size: 1969 4545
XmnI GAAnn'nnTTC
Cuts at: 2987 4643 2987
Size: 1656 4858
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4322 276
Size: 53 83 186 936 2788 2468
Fragments arranged by size:
2788 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI FseI FspAI
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SrfI
SwaI