Restriction map for
pFN21AE0385
(Circular) (Six-base) MAPSORT of: pFN21AE0385.seq Check: 6699 from: 1 to: 7000
pFN21AE0385 7000 bp
With 182 enzymes: SgfI *
February 7, 2012 16:05 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4810 278
Size: 53 83 186 936 3274 2468
Fragments arranged by size:
3274 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2691 4343 1937
Size: 754 1652 4594
Fragments arranged by size:
4594 1652 754
Acc65I G'GTAC_C
Cuts at: 3119 4321 3119
Size: 1202 5798
AclI AA'CG_TT
Cuts at: 1563 5127 5500 1563
Size: 3564 373 3063
Fragments arranged by size:
3564 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2562 3162 5063 6447 6715 1632
Size: 930 600 1901 1384 268 1917
Fragments arranged by size:
1917 1901 1384 930 600 268
AfeI AGC'GCT
Cuts at: 2083 2083
Size: 7000
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6803
AflIII A'CryG_T
Cuts at: 1203 3028 3564 3787 5899 6876 1203
Size: 1825 536 223 2112 977 1327
Fragments arranged by size:
2112 1825 1327 977 536 223
AgeI A'CCGG_T
Cuts at: 5831 5831
Size: 7000
AhdI GACnn_n'nnGTC
Cuts at: 3327 5729 3327
Size: 2402 4598
AleI CACnn'nnGTG
Cuts at: 1166 2326 2616 1166
Size: 1160 290 5550
Fragments arranged by size:
5550 1160 290
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7000
AlwNI CAG_nnn'CTG
Cuts at: 2306 4743 6315 2306
Size: 2437 1572 2991
Fragments arranged by size:
2991 2437 1572
ApaLI G'TGCA_C
Cuts at: 5057 6213 5057
Size: 1156 5844
ApoI r'AATT_y
Cuts at: 1493 2123 2956 3231 3459 4309 4535 4687 6796
Size: 630 833 275 228 850 226 152 2109
Cuts at: 6796 1493
Size: 1697
Fragments arranged by size:
2109 1697 850 833 630 275 228 226
152
AseI AT'TA_AT
Cuts at: 160 1032 3477 3899 4022 5554 160
Size: 872 2445 422 123 1532 1606
Fragments arranged by size:
2445 1606 1532 872 422 123
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7000
AvaI C'yCGr_G
Cuts at: 1870 1943 4325 1870
Size: 73 2382 4545
Fragments arranged by size:
4545 2382 73
AvrII C'CTAG_G
Cuts at: 3203 3203
Size: 7000
BamHI G'GATC_C
Cuts at: 2918 4330 4697 2918
Size: 1412 367 5221
Fragments arranged by size:
5221 1412 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3119 4321 5776 618
Size: 325 217 627 1332 1202 1455 1842
Fragments arranged by size:
1842 1455 1332 1202 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4319 1391
Size: 2928 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2705 928
Size: 971 806 5223
Fragments arranged by size:
5223 971 806
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5191 5225 1903
Size: 34 3254 34 3678
Fragments arranged by size:
3678 3254 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3838 4892 6102 3838
Size: 1054 1210 4736
Fragments arranged by size:
4736 1210 1054
BclI T'GATC_A
Cuts at: 1548 3679 1548
Size: 2131 4869
BfrBI ATG'CAT
Cuts at: 3703 3703
Size: 7000
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2046 5611 136
Size: 107 122 71 631 605 374 3565 1525
Fragments arranged by size:
3565 1525 631 605 374 122 107 71
BglII A'GATC_T
Cuts at: 3258 6995 3258
Size: 3737 3263
BlpI GC'TnA_GC
Cuts at: 4417 4417
Size: 7000
Bme1580I G_kGCm'C
Cuts at: 1790 2756 5061 6217 1790
Size: 966 2305 1156 2573
Fragments arranged by size:
2573 2305 1156 966
BmgBI CAC'GTC
Cuts at: 1318 2029 3031 1318
Size: 711 1002 5287
Fragments arranged by size:
5287 1002 711
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4731 5689 442
Size: 416 53 83 399 24 3314 958 1753
Fragments arranged by size:
3314 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7000
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3265 3265
Size: 7000
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2454 3060 3531 4176 5660 1239
Size: 1215 606 471 645 1484 2579
Fragments arranged by size:
2579 1484 1215 645 606 471
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3257 3642 5125 5990 6288 3257
Size: 385 1483 865 298 3969
Fragments arranged by size:
3969 1483 865 385 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4029 5663 882
Size: 617 2530 1634 2219
Fragments arranged by size:
2530 2219 1634 617
BsaAI yAC'GTr
Cuts at: 493 1204 2866 4791 493
Size: 711 1662 1925 2702
Fragments arranged by size:
2702 1925 1662 711
BsaBI GATnn'nnATC
Cuts at: 4024 6994 4024
Size: 2970 4030
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4051 4807 5189 5824
Size: 53 83 186 936 2518 756 382 635
Cuts at: 5824 275
Size: 1451
Fragments arranged by size:
2518 1451 936 756 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5433 5831 6105 6252 6663 5433
Size: 398 274 147 411 5770
Fragments arranged by size:
5770 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2789 2819 3033 3036 3063
Size: 30 165 30 1386 30 214 3 27
Cuts at: 3063 3066 1178
Size: 3 5112
Fragments arranged by size:
5112 1386 214 165 30 30 30 27
3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 2451 3057 3254 1186
Size: 838 427 606 197 4932
Fragments arranged by size:
4932 838 606 427 197
BseYI C'CCAG_C
Cuts at: 2078 2306 6203 6699 2078
Size: 228 3897 496 2379
Fragments arranged by size:
3897 2379 496 228
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6427
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3451 4450 5211 6239 1540
Size: 275 186 1450 999 761 1028 2301
Fragments arranged by size:
2301 1450 1028 999 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2385 2563 2911 2944 3023 5061 5146 6217 2385
Size: 178 348 33 79 2038 85 1071 3168
Fragments arranged by size:
3168 2038 1071 348 178 85 79 33
BsmI GAATG_Cn'
Cuts at: 3766 4511 4604 3766
Size: 745 93 6162
Fragments arranged by size:
6162 745 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2385 2563 2756 2911 2944 3023 4319
Size: 399 595 178 193 155 33 79 1296
Cuts at: 4319 5061 5146 6217 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1296 1071 742 595 399 193 178
155 85 79 33
BspEI T'CCGG_A
Cuts at: 6663 6663
Size: 7000
BspHI T'CATG_A
Cuts at: 2551 2847 4231 4889 6619 2551
Size: 296 1384 658 1730 2932
Fragments arranged by size:
2932 1730 1384 658 296
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4120 4355 844
Size: 3276 235 3489
Fragments arranged by size:
3489 3276 235
BsrBI CCG'CTC
Cuts at: 1394 1823 4887 1394
Size: 429 3064 3507
Fragments arranged by size:
3507 3064 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2586 5495 5669 65
Size: 1183 1338 2909 174 1396
Fragments arranged by size:
2909 1396 1338 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2036 2065 5644 5831 1700
Size: 110 226 29 3579 187 2869
Fragments arranged by size:
3579 2869 226 187 110 29
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7000
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7000
BssSI C'ACGA_G
Cuts at: 5060 6072 5060
Size: 1012 5988
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7000
BstBI TT'CG_AA
Cuts at: 2148 5804 2148
Size: 3656 3344
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7000
BstXI CCAn_nnnn'nTGG
Cuts at: 3331 3647 3725 3331
Size: 316 78 6606
Fragments arranged by size:
6606 316 78
BstYI r'GATC_y
Cuts at: 1972 2918 3258 3740 4330 4697 5082 5099 6551
Size: 946 340 482 590 367 385 17 1452
Cuts at: 6551 6637 6649 6995 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 946 590 482 385 367 346
340 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2756 3646 3718 3736 513
Size: 552 939 752 890 72 18 3777
Fragments arranged by size:
3777 939 890 752 552 72 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3847 487
Size: 683 56 2621 3640
Fragments arranged by size:
3640 2621 683 56
BtsI GCAGTG_nn'
Cuts at: 4516 5310 5330 4516
Size: 794 20 6186
Fragments arranged by size:
6186 794 20
ClaI AT'CG_AT
Cuts at: 1554 3197 1554
Size: 1643 5357
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4161 4196 529
Size: 35 3597 35 3333
Fragments arranged by size:
3597 3333 35 35
DraI TTT'AAA
Cuts at: 2293 4304 2293
Size: 2011 4989
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6007 809
Size: 507 4691 1802
Fragments arranged by size:
4691 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2034 3448 4447
Size: 54 1425 50 275 18 204 1414 999
Cuts at: 4447 5336 6965 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1414 999 889 275 204 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3448 4447 1537
Size: 275 1636 999 4090
Fragments arranged by size:
4090 1636 999 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4930 1353
Size: 3577 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5587 5961 6107 387
Size: 1064 138 3998 374 146 1280
Fragments arranged by size:
3998 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2454 2562 3060 3162 3531 4176 5063
Size: 393 822 108 498 102 369 645 887
Cuts at: 5063 5660 6447 6715 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 887 822 787 645 597 498 393
369 268 108 102
EcoRI G'AATT_C
Cuts at: 4309 4687 4309
Size: 378 6622
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3140 3140
Size: 7000
FseI GG_CCGG'CC
Cuts at: 2040 2040
Size: 7000
FspI TGC'GCA
Cuts at: 5506 5506
Size: 7000
HaeII r_GCGC'y
Cuts at: 1678 2085 6147 1678
Size: 407 4062 2531
Fragments arranged by size:
4062 2531 407
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3313 3345 3529 3561 3846
Size: 32 135 32 1741 32 184 32 285
Cuts at: 3846 3878 5641 5673 5715 5747 1373
Size: 32 1763 32 42 32 2626
Fragments arranged by size:
2626 1763 1741 285 184 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2692 3570 4344 4590 669
Size: 988 281 754 878 774 246 3079
Fragments arranged by size:
3079 988 878 774 754 281 246
HindIII A'AGCT_T
Cuts at: 748 2718 748
Size: 1970 5030
HpaI GTT'AAC
Cuts at: 3570 4590 3570
Size: 1020 5980
KpnI G_GTAC'C
Cuts at: 3123 4325 3123
Size: 1202 5798
MfeI C'AATT_G
Cuts at: 4599 4599
Size: 7000
MluI A'CGCG_T
Cuts at: 6876 6876
Size: 7000
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3565 6114 6298 1305
Size: 2260 2549 184 2007
Fragments arranged by size:
2549 2260 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5178
Fragments arranged by size:
5178 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2326 2556 2616 3563 3639 3645 3702
Size: 648 1160 230 60 947 76 6 57
Cuts at: 3702 3723 3818 4958 5317 5476 518
Size: 21 95 1140 359 159 2042
Fragments arranged by size:
2042 1160 1140 947 648 359 230 159
95 76 60 57 21 6
MspA1I CmG'CkG
Cuts at: 1606 4416 5093 6241 6486 1606
Size: 2810 677 1148 245 2120
Fragments arranged by size:
2810 2120 1148 677 245
NaeI GCC'GGC
Cuts at: 2038 2038
Size: 7000
NcoI C'CATG_G
Cuts at: 513 1065 2004 2756 3646 3718 513
Size: 552 939 752 890 72 3795
Fragments arranged by size:
3795 939 890 752 552 72
NdeI CA'TA_TG
Cuts at: 387 2155 2571 3699 4206 387
Size: 1768 416 1128 507 3181
Fragments arranged by size:
3181 1768 1128 507 416
NgoMIV G'CCGG_C
Cuts at: 2036 2036
Size: 7000
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7000
NotI GC'GGCC_GC
Cuts at: 4447 4447
Size: 7000
NsiI A_TGCA'T
Cuts at: 3705 3705
Size: 7000
NspI r_CATG'y
Cuts at: 1132 1229 2300 3361 3568 3791 4358 5903 1132
Size: 97 1071 1061 207 223 567 1545 2229
Fragments arranged by size:
2229 1545 1071 1061 567 223 207 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7000
PciI A'CATG_T
Cuts at: 3564 3787 5899 3564
Size: 223 2112 4665
Fragments arranged by size:
4665 2112 223
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7000
PfoI T'CCnGG_A
Cuts at: 2853 2853
Size: 7000
PmeI GTTT'AAAC
Cuts at: 4304 4304
Size: 7000
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5069 5101 6582 6614 5069
Size: 32 1481 32 5455
Fragments arranged by size:
5455 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7000
PsiI TTA'TAA
Cuts at: 3159 4570 3159
Size: 1411 5589
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3819 3851 3819
Size: 32 6968
PstI C_TGCA'G
Cuts at: 830 4352 830
Size: 3522 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7000
SalI G'TCGA_C
Cuts at: 1936 2690 4342 1936
Size: 754 1652 4594
Fragments arranged by size:
4594 1652 754
SbfI CC_TGCA'GG
Cuts at: 4352 4352
Size: 7000
ScaI AGT'ACT
Cuts at: 2637 4222 5248 6960 2637
Size: 1585 1026 1712 2677
Fragments arranged by size:
2677 1712 1585 1026
SfcI C'TryA_G
Cuts at: 826 1045 4348 5483 6164 6355 826
Size: 219 3303 1135 681 191 1471
Fragments arranged by size:
3303 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7000
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7000
SmaI CCC'GGG
Cuts at: 1872 4327 1872
Size: 2455 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3236 3657 5104 6005 6267 820
Size: 197 926 1293 421 1447 901 262 1553
Fragments arranged by size:
1553 1447 1293 926 901 421 262 197
SnaBI TAC'GTA
Cuts at: 493 4791 493
Size: 4298 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7000
SphI G_CATG'C
Cuts at: 1132 4358 1132
Size: 3226 3774
SspI AAT'ATT
Cuts at: 5 52 2289 3903 4924 5
Size: 47 2237 1614 1021 2081
Fragments arranged by size:
2237 2081 1614 1021 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7000
StyI C'CwwG_G
Cuts at: 513 1065 2004 2059 2756 3203 3646 3718 4439
Size: 552 939 55 697 447 443 72 721
Cuts at: 4439 513
Size: 3074
Fragments arranged by size:
3074 939 721 697 552 447 443 72
55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2298 3158 5027 5044 5197 5382 5827
Size: 1255 718 860 1869 17 153 185 445
Cuts at: 5827 325
Size: 1498
Fragments arranged by size:
1869 1498 1255 860 718 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2635 4123 4220
Size: 274 80 33 51 1443 658 1488 97
Cuts at: 4220 5246 6958 96
Size: 1026 1712 138
Fragments arranged by size:
1712 1488 1443 1026 658 274 138 97
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3036 3068 6960 6992 3036
Size: 32 3892 32 3044
Fragments arranged by size:
3892 3044 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7000
XbaI T'CTAG_A
Cuts at: 4336 4336
Size: 7000
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7000
XmaI C'CCGG_G
Cuts at: 1870 4325 1870
Size: 2455 4545
XmnI GAAnn'nnTTC
Cuts at: 5129 5129
Size: 7000
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4808 276
Size: 53 83 186 936 3274 2468
Fragments arranged by size:
3274 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FalI FseI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PsrI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI BaeI BbeI BbvCI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I
EcoRV FspAI KasI NarI NruI PacI PmlI PpuMI
PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI