Restriction map for
pFN21AE0382
(Circular) (Six-base) MAPSORT of: pFN21AE0382.seq Check: 9813 from: 1 to: 6835
pFN21AE0382 6835 bp
With 182 enzymes: SgfI *
April 15, 2010 09:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2454 4645 278
Size: 53 83 186 936 918 2191 2468
Fragments arranged by size:
2468 2191 936 918 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2040 2454 4178 1937
Size: 103 414 1724 4594
Fragments arranged by size:
4594 1724 414 103
Acc65I G'GTAC_C
Cuts at: 3626 4156 3626
Size: 530 6305
AclI AA'CG_TT
Cuts at: 1563 4962 5335 1563
Size: 3399 373 3063
Fragments arranged by size:
3399 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2128 4898 6282 6550 1632
Size: 496 2770 1384 268 1917
Fragments arranged by size:
2770 1917 1384 496 268
AfeI AGC'GCT
Cuts at: 2275 2275
Size: 6835
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6638
AflIII A'CryG_T
Cuts at: 1203 5734 6711 1203
Size: 4531 977 1327
Fragments arranged by size:
4531 1327 977
AgeI A'CCGG_T
Cuts at: 5666 5666
Size: 6835
AhdI GACnn_n'nnGTC
Cuts at: 5564 5564
Size: 6835
AleI CACnn'nnGTG
Cuts at: 1166 2183 3932 1166
Size: 1017 1749 4069
Fragments arranged by size:
4069 1749 1017
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3950 1184
Size: 2766 4069
AlwNI CAG_nnn'CTG
Cuts at: 3082 3520 3529 3610 4578 6150 3082
Size: 438 9 81 968 1572 3767
Fragments arranged by size:
3767 1572 968 438 81 9
ApaI G_GGCC'C
Cuts at: 3410 3576 3410
Size: 166 6669
ApaLI G'TGCA_C
Cuts at: 2863 3600 4892 6048 2863
Size: 737 1292 1156 3650
Fragments arranged by size:
3650 1292 1156 737
ApoI r'AATT_y
Cuts at: 1493 4011 4144 4370 4522 6631 1493
Size: 2518 133 226 152 2109 1697
Fragments arranged by size:
2518 2109 1697 226 152 133
AseI AT'TA_AT
Cuts at: 160 1032 5389 160
Size: 872 4357 1606
Fragments arranged by size:
4357 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6835
AvaI C'yCGr_G
Cuts at: 1870 1943 2226 2247 2258 3503 3567 4160 1870
Size: 73 283 21 11 1245 64 593 4545
Fragments arranged by size:
4545 1245 593 283 73 64 21 11
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3491 3524 3491
Size: 33 6802
BamHI G'GATC_C
Cuts at: 3356 4165 4532 3356
Size: 809 367 5659
Fragments arranged by size:
5659 809 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3547 3626 4156 5611 618
Size: 325 217 627 1760 79 530 1455 1842
Fragments arranged by size:
1842 1760 1455 627 530 325 217 79
BanII G_rGCy'C
Cuts at: 1391 2054 3410 3576 4132 4154 1391
Size: 663 1356 166 556 22 4072
Fragments arranged by size:
4072 1356 663 556 166 22
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2839 3370 928
Size: 971 940 531 4393
Fragments arranged by size:
4393 971 940 531
BbvCI CC'TCA_GC
Cuts at: 3858 3858
Size: 6835
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5026 5060 1903
Size: 34 3089 34 3678
Fragments arranged by size:
3678 3089 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3056 3492 3548 4727 5937 3056
Size: 436 56 1179 1210 3954
Fragments arranged by size:
3954 1210 1179 436 56
BclI T'GATC_A
Cuts at: 1548 2691 1548
Size: 1143 5692
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5446 136
Size: 107 122 71 631 605 3774 1525
Fragments arranged by size:
3774 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2367 3049 6830 2367
Size: 682 3781 2372
Fragments arranged by size:
3781 2372 682
BlpI GC'TnA_GC
Cuts at: 2138 4252 2138
Size: 2114 4721
Bme1580I G_kGCm'C
Cuts at: 1790 2867 3410 3576 3604 4896 6052 1790
Size: 1077 543 166 28 1292 1156 2573
Fragments arranged by size:
2573 1292 1156 1077 543 166 28
BmgBI CAC'GTC
Cuts at: 1318 2659 3019 1318
Size: 1341 360 5134
Fragments arranged by size:
5134 1341 360
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2740 4566 5524
Size: 416 53 83 399 24 1323 1826 958
Cuts at: 5524 442
Size: 1753
Fragments arranged by size:
1826 1753 1323 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6835
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2761 2761
Size: 6835
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2038 2290 2786 3464 3664 3839 4040 5495
Size: 799 252 496 678 200 175 201 1455
Cuts at: 5495 1239
Size: 2579
Fragments arranged by size:
2579 1455 799 678 496 252 201 200
175
Bpu10I CC'TnA_GC
Cuts at: 2881 3858 2881
Size: 977 5858
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2051 4960 5825 6123 2051
Size: 2909 865 298 2763
Fragments arranged by size:
2909 2763 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2186 5498 882
Size: 617 687 3312 2219
Fragments arranged by size:
3312 2219 687 617
BsaAI yAC'GTr
Cuts at: 493 1204 2955 4626 493
Size: 711 1751 1671 2702
Fragments arranged by size:
2702 1751 1671 711
BsaBI GATnn'nnATC
Cuts at: 6829 6829
Size: 6835
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2091 2451 4642 5024
Size: 53 83 186 936 558 360 2191 382
Cuts at: 5024 5659 275
Size: 635 1451
Fragments arranged by size:
2191 1451 936 635 558 382 360 186
83 53
BsaWI w'CCGG_w
Cuts at: 2195 3042 5268 5666 5940 6087 6498 2195
Size: 847 2226 398 274 147 411 2532
Fragments arranged by size:
2532 2226 847 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3674 3704 3990 4020 1178
Size: 30 165 30 2271 30 286 30 3993
Fragments arranged by size:
3993 2271 286 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3380 3689 1186
Size: 2194 309 4332
Fragments arranged by size:
4332 2194 309
BseYI C'CCAG_C
Cuts at: 2218 2404 2646 2841 3096 3403 4124 6038 6534
Size: 186 242 195 255 307 721 1914 496
Cuts at: 6534 2218
Size: 2519
Fragments arranged by size:
2519 1914 721 496 307 255 242 195
186
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2692 3910 1163
Size: 573 956 1218 4088
Fragments arranged by size:
4088 1218 956 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4285 5046 6074 1540
Size: 275 186 2284 761 1028 2301
Fragments arranged by size:
2301 2284 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2054 2073 2867 3604 4896 4981 6052 2054
Size: 19 794 737 1292 85 1071 2837
Fragments arranged by size:
2837 1292 1071 794 737 85 19
BsmI GAATG_Cn'
Cuts at: 4346 4439 4346
Size: 93 6742
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2054 2073 2867 3410 3576 3604 4132
Size: 399 264 19 794 543 166 28 528
Cuts at: 4132 4154 4896 4981 6052 1391
Size: 22 742 85 1071 2174
Fragments arranged by size:
2174 1071 794 742 543 528 399 264
166 85 28 22 19
BspEI T'CCGG_A
Cuts at: 3042 6498 3042
Size: 3456 3379
BspHI T'CATG_A
Cuts at: 2521 3381 4724 6454 2521
Size: 860 1343 1730 2902
Fragments arranged by size:
2902 1730 1343 860
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2052 2466 3067 3528 4190 844
Size: 1208 414 601 461 662 3489
Fragments arranged by size:
3489 1208 662 601 461 414
BsrBI CCG'CTC
Cuts at: 1394 1823 3697 4722 1394
Size: 429 1874 1025 3507
Fragments arranged by size:
3507 1874 1025 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5330 5504 65
Size: 1183 4082 174 1396
Fragments arranged by size:
4082 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5479 5666 1700
Size: 110 3669 187 2869
Fragments arranged by size:
3669 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6835
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6835
BssSI C'ACGA_G
Cuts at: 4895 5907 4895
Size: 1012 5823
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3529 3655 794
Size: 2735 126 3974
Fragments arranged by size:
3974 2735 126
BstBI TT'CG_AA
Cuts at: 5639 5639
Size: 6835
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6835
BstXI CCAn_nnnn'nTGG
Cuts at: 3583 3583
Size: 6835
BstYI r'GATC_y
Cuts at: 1972 2367 2595 3049 3356 3436 4165 4532 4917
Size: 395 228 454 307 80 729 367 385
Cuts at: 4917 4934 6386 6472 6484 6830 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 729 454 395 385 367 346
307 228 86 80 17 12
Bsu36I CC'TnA_GG
Cuts at: 3363 3363
Size: 6835
BtgI C'CryG_G
Cuts at: 513 1065 2004 2113 513
Size: 552 939 109 5235
Fragments arranged by size:
5235 939 552 109
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3748 487
Size: 683 56 2522 3574
Fragments arranged by size:
3574 2522 683 56
BtsI GCAGTG_nn'
Cuts at: 2785 2804 3115 4351 5145 5165 2785
Size: 19 311 1236 794 20 4455
Fragments arranged by size:
4455 1236 794 311 20 19
ClaI AT'CG_AT
Cuts at: 1554 2949 1554
Size: 1395 5440
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6800
DraI TTT'AAA
Cuts at: 4139 4139
Size: 6835
DraIII CAC_nnn'GTG
Cuts at: 2392 2929 3655 2392
Size: 537 726 5572
Fragments arranged by size:
5572 726 537
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3787 4112 5842 809
Size: 507 2471 325 1730 1802
Fragments arranged by size:
2471 1802 1730 507 325
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2116 2344 2631
Size: 54 1425 50 275 18 286 228 287
Cuts at: 2631 2784 3238 3492 3692 3993 4282 5171 6800
Size: 153 454 254 200 301 289 889 1629
Cuts at: 6800 8
Size: 43
Fragments arranged by size:
1629 1425 889 454 301 289 287 286
275 254 228 200 153 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4282 1537
Size: 275 2470 4090
Fragments arranged by size:
4090 2470 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2562 3771 4765 1353
Size: 1209 1209 994 3423
Fragments arranged by size:
3423 1209 1209 994
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5422 5796 5942 387
Size: 1064 138 3833 374 146 1280
Fragments arranged by size:
3833 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2052 2052
Size: 6835
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2038 2128 2290 2786 3464 3664 3839
Size: 393 406 90 162 496 678 200 175
Cuts at: 3839 4040 4898 5495 6282 6550 1239
Size: 201 858 597 787 268 1524
Fragments arranged by size:
1524 858 787 678 597 496 406 393
268 201 200 175 162 90
EcoNI CCTnn'n_nnAGG
Cuts at: 3643 3643
Size: 6835
EcoO109I rG'GnC_Cy
Cuts at: 2223 3407 3517 3572 3607 3976 2223
Size: 1184 110 55 35 369 5082
Fragments arranged by size:
5082 1184 369 110 55 35
EcoRI G'AATT_C
Cuts at: 4144 4522 4144
Size: 378 6457
EcoRV GAT'ATC
Cuts at: 3811 3811
Size: 6835
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2333 3438 4126 2333
Size: 1105 688 5042
Fragments arranged by size:
5042 1105 688
FspI TGC'GCA
Cuts at: 5341 5341
Size: 6835
HaeII r_GCGC'y
Cuts at: 1678 2277 3322 3875 5982 1678
Size: 599 1045 553 2107 2531
Fragments arranged by size:
2531 2107 1045 599 553
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2285 2317 2599 2631 2966
Size: 32 135 32 713 32 282 32 335
Cuts at: 2966 2998 3870 3902 3990 4022 5476 5508 5550
Size: 32 872 32 88 32 1454 32 42
Cuts at: 5550 5582 1373
Size: 32 2626
Fragments arranged by size:
2626 1454 872 713 335 282 135 88
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2041 2985 4179 4425 669
Size: 988 281 103 944 1194 246 3079
Fragments arranged by size:
3079 1194 988 944 281 246 103
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6835
HpaI GTT'AAC
Cuts at: 4425 4425
Size: 6835
KpnI G_GTAC'C
Cuts at: 3630 4160 3630
Size: 530 6305
MfeI C'AATT_G
Cuts at: 4434 4434
Size: 6835
MluI A'CGCG_T
Cuts at: 6711 6711
Size: 6835
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2322 2915 5949 6133 1305
Size: 1017 593 3034 184 2007
Fragments arranged by size:
3034 2007 1017 593 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2346 2786 3240 3494 10
Size: 54 1425 343 514 440 454 254 3351
Fragments arranged by size:
3351 1425 514 454 440 343 254 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2183 2870 3299 3621 3752 3905 3932
Size: 648 1017 687 429 322 131 153 27
Cuts at: 3932 4793 5152 5311 518
Size: 861 359 159 2042
Fragments arranged by size:
2042 1017 861 687 648 429 359 322
159 153 131 27
MspA1I CmG'CkG
Cuts at: 1606 2115 2404 2650 3478 3526 3924 4251 4928
Size: 509 289 246 828 48 398 327 677
Cuts at: 4928 6076 6321 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 828 677 509 398 327 289
246 245 48
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5344
Fragments arranged by size:
5344 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6835
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6835
NotI GC'GGCC_GC
Cuts at: 4282 4282
Size: 6835
NspI r_CATG'y
Cuts at: 1132 1229 4193 5738 1132
Size: 97 2964 1545 2229
Fragments arranged by size:
2964 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6835
PciI A'CATG_T
Cuts at: 5734 5734
Size: 6835
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6835
PfoI T'CCnGG_A
Cuts at: 2054 3432 3441 2054
Size: 1378 9 5448
Fragments arranged by size:
5448 1378 9
PmeI GTTT'AAAC
Cuts at: 4139 4139
Size: 6835
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4904 4936 6417 6449 4904
Size: 32 1481 32 5290
Fragments arranged by size:
5290 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2223 3517 3976 2223
Size: 1294 459 5082
Fragments arranged by size:
5082 1294 459
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6835
PsiI TTA'TAA
Cuts at: 4405 4405
Size: 6835
PspOMI G'GGCC_C
Cuts at: 3406 3572 3406
Size: 166 6669
PstI C_TGCA'G
Cuts at: 830 2049 2481 2724 3483 3525 4187 830
Size: 1219 432 243 759 42 662 3478
Fragments arranged by size:
3478 1219 759 662 432 243 42
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6835
PvuII CAG'CTG
Cuts at: 3478 3526 3924 3478
Size: 48 398 6389
Fragments arranged by size:
6389 398 48
SacI G_AGCT'C
Cuts at: 2054 2054
Size: 6835
SacII CC_GC'GG
Cuts at: 2116 2116
Size: 6835
SalI G'TCGA_C
Cuts at: 1936 2039 4177 1936
Size: 103 2138 4594
Fragments arranged by size:
4594 2138 103
SanDI GG'GwC_CC
Cuts at: 2223 2223
Size: 6835
SbfI CC_TGCA'GG
Cuts at: 4187 4187
Size: 6835
ScaI AGT'ACT
Cuts at: 5083 6795 5083
Size: 1712 5123
SfcI C'TryA_G
Cuts at: 826 1045 2045 2477 2720 2792 3479 3521 3709
Size: 219 1000 432 243 72 687 42 188
Cuts at: 3709 4183 5318 5999 6190 826
Size: 474 1135 681 191 1471
Fragments arranged by size:
1471 1135 1000 687 681 474 432 243
219 191 188 72 42
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6835
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6835
SmaI CCC'GGG
Cuts at: 1872 2249 2260 4162 1872
Size: 377 11 1902 4545
Fragments arranged by size:
4545 1902 377 11
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2030 4939 5840 6102 820
Size: 197 926 87 2909 901 262 1553
Fragments arranged by size:
2909 1553 926 901 262 197 87
SnaBI TAC'GTA
Cuts at: 493 2955 4626 493
Size: 2462 1671 2702
Fragments arranged by size:
2702 2462 1671
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6835
SphI G_CATG'C
Cuts at: 1132 4193 1132
Size: 3061 3774
SspI AAT'ATT
Cuts at: 5 52 4759 5
Size: 47 4707 2081
Fragments arranged by size:
4707 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6835
StyI C'CwwG_G
Cuts at: 513 1065 2004 2380 2432 3229 3979 4274 513
Size: 552 939 376 52 797 750 295 3074
Fragments arranged by size:
3074 939 797 750 552 376 295 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2979 3575 4862 4879 5032 5217 5662
Size: 1255 1399 596 1287 17 153 185 445
Cuts at: 5662 325
Size: 1498
Fragments arranged by size:
1498 1399 1287 1255 596 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5081 6793 96
Size: 274 80 33 51 1443 3104 1712 138
Fragments arranged by size:
3104 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2764 2796 3573 3605 3674 3706 6795 6827 2764
Size: 32 777 32 69 32 3089 32 2772
Fragments arranged by size:
3089 2772 777 69 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2450 1316
Size: 1134 5701
XbaI T'CTAG_A
Cuts at: 4171 4171
Size: 6835
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 3583 3904 2011
Size: 1572 321 4942
Fragments arranged by size:
4942 1572 321
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6835
XmaI C'CCGG_G
Cuts at: 1870 2247 2258 4160 1870
Size: 377 11 1902 4545
Fragments arranged by size:
4545 1902 377 11
XmnI GAAnn'nnTTC
Cuts at: 3638 4964 3638
Size: 1326 5509
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2452 4643 276
Size: 53 83 186 936 918 2191 2468
Fragments arranged by size:
2468 2191 936 918 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SacII SalI
SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BbeI BfrBI BsiWI BsmBI
BstZ17I FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspXI PsrI RsrII SapI SexAI
SfiI SfoI SrfI SwaI